Objective To explore relativity between serum cortisol and post-stroke depression in old patients with stroke. Methods Serum cortisols of 41 stroke patients were examined two weeks after stroke, and they were all assessed through Hamilton Depression Scale and then happening rates of post-stroke depression observed in both normal cortisol group and enhanced cortisol group were tested through χ2 test. Meanwhile relativity between serum cortisol level and post-stroke depression were analysised. Results Happening rates of post-stroke depression had an evidently difference between both normal cortisol group and enhanced cortisol group(χ2=4.11;P <0.05). Mark of normal cortisol group was 15.29±7.28, enhanced cortisol group was 25.85±10.81, both groups had an evidently difference( P <0.01). Serum cortisols were not obviously relative to depression degree in normal cortisol group( r =0.295;P =0.194) while they were obviously relative in enhanced cortisol group( r =0.702;P =0.001). Conclusion Enhanced cortisol could forecast higher possibility of depression after the old post-stroke, and detecting cortisol could offer theory foundation for prevention and cure of post-stroke depression.

Objective To explore the effect of erythropoietin(EPO) on Bcl-xl expression in hippocampal CA1 subregion and cognizing function in rats with cerebral ischemia-reperfusion(IR).Methods Male SD rats were randomly separated into sham-operated group,IR group and EPO group,and the IR models were made.At 3 h before IR models made,rHu-EPO and saline were injected into rat lateral ventricles in EPO group and IR group,respectively,with help of stereotaxic coordinates.Bcl-xl protein expression of Hippocampal CA1 subregion was detected by immunohistochemistry at 24 h after operation.The learning and memory abilities were examined by electricity maze at 4 weeks after operation.Results Compared to sham-operated group,Bcl-xl protein expression in EPO group and IR group were obviously decreased,but EPO group was significantly increased than IR group(all P

Objective To explore the protecting mechanism of erythropoietin (EPO) on learning and memory of Alzheimer disease (AD) rats. Methods The AD model was made by injected into rat hippocampal CA1 subregion with amyloid beta-protein(Aβ). Male Spraque-Dawley rats were as the experimental objects, which were randomly separated into 3 groups including Sham, Saline control and EPO treatment. After Aβ was injected into rats hippocampal CA1 subregion ,saline or EPO was respectively injected into the lateral ventricle of rats,with help of stereotaxic coordinates, upon the designed conditions. Hippocampal CA1 subregion Bcl-xl expression changes were observed 24 hours after the operation, and learning and memory abilities were checked 4 weeks after the operation. Results 24 hours after the operation Bcl-xl expression in the EPO group and the Saline group was less than the Sham control ,while Bcl-xl positive cells( 100.42 ± 12.43/field) in the EPO group were more than in the Saline group( 82.06 ± 19.68/field ) (P ＜ 0. 05 ). 4 weeks after the operation learning ability in the EPO group ( 20.38 ± 5.88 ) was better than Saline group ( 25.50 - 3.25 ) (P ＜ 0. 05 ), and memory ability in the EPO group (4.75 ± 1.75 ) was better than the Saline group(2.88 ± 1.55 )(P ＜ 0.05 ). Conclusion EPO could improve the learning and memory abilities in the model rats,and it could be related with EPO restraining Bcl-xl expression decreasing.

Objective: This study aimed to translate the English version of the Perceived Competence Scale for Disaster Mental Health Workforce (PCS-DMHW) into Chinese, and to test its reliability and validity in Chinese mental health workers. Methods: With the consent of Professor Choi, Keimyung University, Korea, and the authorization of the scale, the English version of PCS-DMHW was translated, retranslated and culturally debugged to form the Chinese version of PCS-DMHW. The general information questionnaire and the Chinese version of PCS-DMHW scale were used to investigate 706 mental health workforce from 9 tertiary hospitals in Sichuan province in China from March 24, 2020 to April 14, 2020. The Cronbach’s α coefficient was used to evaluate the internal consistency reliability of the scale, and the test-retest correlation coefficient r was used to evaluate the test-retest reliability of the scale. The content validity indexes (CVI) and exploratory factor analysis (EFA) was used separately for evaluating the content validity and structure validity of the scale. Results: The Cronbach’s α coefficient of the Chinese version of PCS-DMHW total scale, individual competences and organizational competences subscale was 0.978, 0.956, and 0.964, respectively. The test-retest reliability of the total scale, individual competences and organizational competences subscale was 0.949, 0.932 and 0.927, respectively. The item-level CVI of all scale were ranged from 0.833–1.000, the scale-level CVI (S-CVI)/universal agreement of the total scale, individual competences and organizational competences subscale was 0.833, 0.875, and 0.857, respectively, and the S-CVI/average was 0.972, 0.979, and 0.976, respectively. EFA showed that two principal components were extracted from the subscale of individual competences and organizational competences. Conclusion The Chinese version of PCS-DMHW has good reliability and validity, and can be widely used in China.

Objective: To construct a scientific, practical and objective quality evaluation system for evaluating clinical probation teaching effects of Psychiatry Nursing in undergraduate students. Methods: Protocol of the system was initially made via literature analysis method and experts interview method; indexes were screened via Delphi method; and the evaluation system was formed by comprehensively considering experts' suggestions. The data was analyzed by using SPSS 19.0. Results: All expert authority coefficients were more than 0.8; expert activeness coefficients in two rounds were 100% and 88.3%, respectively; and expert coordination coefficient was 0.235 (P<0.01). The designed teaching evaluation system contained three first-level indexes, seven second-level indexes and forty-one third-level indexes. Conclusion The drafted teaching evaluation system for evaluating clinical probation teaching effects of Psychiatric Nursing reflects requirements and characteristics of psychiatric special training during clinical probation and pays attention to cultivate students' humanistic care and clinical practice ability, with high scientificness and reliability, so as to provide objective and quantitative basis for evaluating probation.

Objective To investigate the changes in CB1R expression in various regions of the hippocampus of rats at different time points after status epilepticus.Methods Use lithium chloride-pilocarpine to induce status epilepticus（SE）in rats.At 1 w，2 w，3 w，and 4 w after SE，rat hippocampal slices were taken，and the cannabinoid type 1 receptor （CB1R） immunohistochemical staining was performed to measure the CA1 stratum radiatum，CA-3 stratum lucidum，and stratum moleculare CB1R mean integrated optical density value（MIOD），all values are statistically analyzed. At the same time，hippocampal slices were taken 4 w after SE and stained with NeuN and GFAP to observe the changes of hippocampal neurons and astrocytes.Results In the stratum radiatum of hippocampal CA-1 area，the expression of CB1R at each time point after SE was higher than that of the control（P<0.05），and the expression of CB1R 4 w after SE was higher than 1 w，2 w，3 w after SE （P<0.05）；In the stratum lucidum of hippocampal CA-3 area，the expression of CB1R at each time point after SE was higher than that of the control （P<0.05），and the expression of CB1R 4W after SE was higher than 1 w after SE （P<0.05）；In the hippocampus stratum moleculare，the expression of CB1R at each time point after SE was higher than that of the control（P<0.05），but the expression of CB1R was not statistically different between 1 w，2 w，3 w，and 4 w after SE （P>0.05）. Neurons in the stratum pyramidale of the hippocampal CA-1 area were significantly lost，and astrocytes proliferated and enlarged 4 w after SE.Conclusion Rats had the characteristics of sclerotic hippocampus at 4 w after SE. After SE，the expression of CB1R in stratum radiatum of CA1 area，stratum lucidum of CA3 area，and stratum moleculare of the rat hippocampus increased. In the CA1 area’s stratum radiatum and CA3 area’s stratum lucidum CB1R increased most significantly at 4 w after SE.The stratum moleculare CB1R increased at 1 w after SE and the increase was not obvious later.