Objective To investigate the college students′understanding of antimicrobial agents knowledge in two colleges of Huainan and provide a scientific basis to further conduct the health education of rational use of anti -microbial drugs among college students .Methods The antimicrobial perception of 2 college students with 10 differ-ent majors were investigated by cluster-sampled with the questionnaires designed by the authors .Results The correct answer rate of antimicrobial agents knowledge was 61.7%in overall college students ,72.3%in pharmaceutical pro-fessional class students,53.4%in non-medical specialty,and the difference was statistically significant (P<0.01). In lower grade students,the rate was 57.2%and 68.1%in high school students.Also the difference was statistically significant (P<0.01).The rate of students who use antimicrobial drugs without guidance reached 84.1%,the rate in pharmaceutical professional class students (88.6%) was higher than that in non-medical professional class students (80.6%) (P<0.01).Conclusion College students are not sufficiently aware of using antibiotics reasonably and adverse reaction .Consequently ,related publicity and education should be strengthened in various forms to improve lev-el of using antibiotics reasonably among college students .