Objective To study the effect of arsenic trioxide(As_2O_3) on reactive oxygen species(ROS) of human hepatocellular carcinoma cell(HCC) line.Methods The MTT method was used to evaluate the influence of As_2O_3 on proliferation of HCC.ROS was detected by flow cytometry.Results As_2O_3 could obviously decrease the proliferation rate of HCC cell line in dose-dependent and time-dependent manner.The analysis of flow cytometry showed As_2O_3 could obviously increase ROS in dose-dependent and time-dependent manner as well.Conclusion As_2O_3 can inhibit the proliferation of hepatic cell carcinoma SMMC-7721 cell line significantly,and the anti-tumours mechanism of arsenic trioxide may be that it could induce SMMC-7721 cell apoptosis by increasing ROS level.

Objective:To observe the clinical efficacy of electroacupuncture (EA) combined with tuina for chronic tension-type headache (CTTH). Methods:A total of 97 CTTH cases were randomly allocated into an observation group (n=52) and a control group (n=45). Patients in the observation group were treated with EA plus tuina based on pattern identification, whereas patients in the control group were treated with oral amitriptyline and oryzanol. Patients in both groups were treated for 8 weeks. A follow-up was conducted 3 months after the treatment. The intensity, duration, and frequency of the headache were recorded and compared before and after the treatment. Additionally, the patients' psychological state and quality of life (QOL) were compared between the two groups. Results:There were intra-group statistically significant differences in headache intensity score, headache duration, and headache frequency after the treatment and during the follow-up compared with those before the treatment (allP<0.05); and there were between-group statistically significant differences during the same time frame (allP<0.05). The Hamilton depression scale-17 items (HAMD-17) and Hamilton anxiety scale (HAMA) scores were significantly reduced in both groups after the treatment or during the follow-up (allP<0.01); and there were no between-group statistical significances during the same time frame (allP>0.05). The World Health Organization quality of life-BREF (WHOQOL-BREF) scores were significantly reduced in both groups after the treatment or during the follow-up (allP<0.05); and the scores in the observation group were significantly lower than those in the control group (allP<0.05) during the same time frame. The total effective rate was 88.0% in the observation group, versus 71.4% in the control group, showing a significant difference (P<0.05). Conclusion:EA combined with tuina can significantly decrease the frequency of chronic tension-type headache, alleviate headache intensity, shorten headache duration, and improve the patients' wellness. It is better than amitriptyline plus oryzanol.

Objective To elucidate the neuromechanism of Chinese words comprehension with spatiotemporal pattern of event-related potential (ERP).Methods Fifteen people were recruited during 2011 and 2012.After using Chinese words as stimulating materials,each subject was required to judge whether a target Chinese word belongs to the same class as semantic match or mismatch.EEG was recorded using an ERP system of 19 channels developed.Statistical parametric mapping of t-valus [SPM (t)] was gained from interpolation calculated by each channel's t-value.Results Under two classes,the ERP of 19 channels showed N200,N400 and P600 elements:N200 might be distributed in the bilateral occipital and post-temporal areas,and N400 and P600 might be widely distributed in the double top central-occipital and temporal region.SPM(t) presented differences in multiple regions in 3 stages:during 100-300 ms,the early left hemispheric significant positive deflections 240 ms in left temporoccipital and left frontal regions; during 300-400 ms (the N400 stage),negative deflections in right post-temporal,and both centroparietal and left frontal regions; during 440-580 ms (P600 stage),significant positive deflections in both occipitaloparietal and right temporal regions.Conclusion The positive effects within left temporoccipital and left frontal regions seem to reflect visual word processing and initial syntactic processing; the N400 effects within right post-temporal,both centroparietal and left frontal regions seem to reflect integrate semantic analysis processing,and P600 effects within occipitaloparietal and right temporal regions seem to reflect reanalysis and the integration of semantic and syntactic to obtain the overall meanings of Chinese sentences