1.Clinical study on mild cognitive impairment converting to dementia
Chinese Journal of Neurology 1999;0(06):-
Objective To study the natural history of mild cognitive impairment (MCI) progress to dementia and to evaluate the efficacy of acetylcholinesterase inhibitor (AChEI) donepezil on MCI.Methods Ninty-eight patients with MCI including amnestic MCI and non-amnestic MCI were retrospectively analyzed. The patients with donepezil or not were separately divided to two groups while they were matched by sex, age,degree of MCI and possession of the ApoE?4 allele.The rate of conversion from MCI to dementia, measured with the Mini-Mental State Examination (MMSE),Alzheimer's Disease Assessment Scale- Cognitive section(ADAS-Cog) and Wechsler Memory Scale-Revised (WMS-R),was compared between the two groups.Results In aMCI group,the rate of conversion dropped by 15.1% and 8.3% (P0.05).Conclusion Donepezil could postpone the conversion of MCI to dementia.
2.Effects of repeated intraperitoneal dexmedetomidine on cognitive function in rats with chronic cerebral ischemia
Fujuan HE ; Chengyao WANG ; Shuyue XIAN ; Mian PENG ; Yang XU
Chinese Journal of Anesthesiology 2012;(10):1208-1210
Objective To investigate the effects of repeated intraperitoneal dexmedetomidine on the cognitive function in rats with chronic cerebral ischemia.Methods Forty-eight male Sprague-Dawley rats,aged 3-4months,weighing 250-300 g,were randomly divided into 4 groups (n =12 each) ∶ sham operation group (group S),chronic cerebral ischemia group (group IS),dexmedetomidine treatment 1 group (group DXM1) and dexmedetomidine treatment 2 group (group DXM2).Dexmedetomidine 5 μg/kg was injected intraperitoneally at 30 min before occlusion of bilateral common carotid arteries and 3,12,24 and 48 h after occlusion in group DXM1,and at 3,12,24 and 48 h after occlusion in group DXM2.The cognitive function was assessed by Morris water maze 2 weeks after occlusion.The apoptosis was examined by TUNEL.The expression of Bcl-2 protein in hippocampus was detected by Western blot.Results Compared with group S,the escape latency was significantly prolonged from 2nd day to 5th day after the place navigation test in group IS and on 2nd day after Morris water maze test in groups DXM1 and DXM2,and the time of staying in 1 st quadrant was significantly shortened,the apoptotic rate was increased,and the expression of Bcl-2 was up-regulated in groups IS,DXM1 and DXM2 (P < 0.05).Compared with group IS,the escape latency was significantly shortened from 3rd day to 5th day after the place navigation
3.Determination of fibrinogen content in cryoprecipitated antihemophilic factor by fluorescence quenching method
Jing LAN ; Na FENG ; Wenjuan ZHANG ; Jin DING ; Peng PENG ; Na LIU ; Xin CAO ; Hua XU
Chinese Journal of Blood Transfusion 2022;35(12):1208-1212
【Objective】 To establish a new method for the determination of fibrinogen content in cryoprecipitated antihemophilic factor. 【Methods】 Fibrinogen (Fib) could bind with sheep anti-human fibrinogen (anti-Fib) specifically and further form antigen-antibody complex. When the Fib was present in the solution, the fluorescence of fluorescein isothiocyanate (FITC) labeled on the anti-Fib (FITC-anti-Fib) was quenched due to the formation of immune complex. The fluorescence quenching degree of FITC-anti-Fib was positively correlated with Fib concentration (cFib) in a certain concentration range. 【Results】 The linear relationship between fluorescence quenching degree [(I
4.Determination of suspended erythrocyte volume range and establishment of its internal control standard
Jing LAN ; Hua XU ; Xin CAO ; Jiawei LI ; Jin DING ; Peng PENG ; Na LIU ; Na FENG
Chinese Journal of Blood Transfusion 2023;36(3):263-265
【Objective】 To determine the volume range of suspended erythrocyte and establish its internal control standard. 【Methods】 The theoretical value of suspended erythrocyte volume was calculated according to the screening criteria of healthy blood donors and Quality Requirements for Whole Blood and Blood Components. A total of 2 410 bags of 1 U and 2 U suspended erythrocyte were randomly selected and weighed, and the volume range were formulated by
5.Cause analysis, prevention, and treatment of postoperative restlessness after general anesthesia in children with cleft palate.
Hao XU ; Xiao Peng MEI ; Li Xian XU
Journal of Dental Anesthesia and Pain Medicine 2017;17(1):13-20
Cleft palate is one of the most common congenital malformations of the oral and maxillofacial region, with an incidence rate of around 0.1%. Early surgical repair is the only method for treatment of a cleft lip and palate. However, because of the use of inhalation anesthesia in children and the physiological characteristics of the cleft palate itself combined with the particularities of cleft palate surgery, the incidence rate of postoperative emergence agitation (EA) in cleft palate surgery is significantly higher than in other types of interventions. The exact mechanism of EA is still unclear. Although restlessness after general anesthesia in children with cleft palate is self-limiting, its effects should be considered by clinicians. In this paper, the related literature on restlessness after surgery involving general anesthesia in recent years is summarized. This paper focuses on induction factors as well as prevention and treatment of postoperative restlessness in children with cleft palate after general anesthesia. The corresponding countermeasures to guide clinical practice are also presented in this paper.
Anesthesia, General*
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Anesthesia, Inhalation
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Child*
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Cleft Lip
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Cleft Palate*
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Dihydroergotamine
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Humans
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Incidence
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Methods
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Palate
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Psychomotor Agitation*
6.The effect of pre- and after-treatment of sevoflurane on central ischemia tolerance and the underlying mechanisms
Hao XU ; Xiao Peng MEI ; Li Xian XU
Journal of Dental Anesthesia and Pain Medicine 2018;18(1):1-8
In recent years, with continuous research efforts targeted at studying the effects of pre- and after-treatment of inhaled anesthetics, significant progress has been made regarding the common clinical use of low concentrations of inhaled sevoflurane and its effect on induced central ischemia tolerance by pre- and post-treatment. In this study, we collected, analyzed, classified, and summarized recent literature regarding the effect of sevoflurane on central ischemia tolerance and its related mechanisms. In addition, we provide a theoretical basis for the clinical application of sevoflurane to protect the central nervous system and other important organs against ischemic injury.
Anesthetics
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Central Nervous System
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Ischemia
7.Determination of methylene blue release in plasma virus inactivation bag by fluorescence energy transfer method
Jing LAN ; Yi GUO ; Jin DING ; Yuan ZHANG ; Na FENG ; Peng PENG ; Na LIU ; Xin CAO ; Hua XU
Chinese Journal of Blood Transfusion 2022;35(3):242-245
【Objective】 To establish a simple, economical and rapid method for the determination of methylene blue (MB) release in virus inactivation bag. 【Methods】 Based on the fluorescence energy transfer between MB and BSA-stabilized gold nanoclusters (BSA-AuNCs), the standard curve of MB determination was established by measuring the fluorescence quenching degree of MB to BSA-AuNCs in different concentrations to conduct the determination of MB release in virus inactivation bag. 【Results】 There was a good linear relationship between the MB concentration (c
8.The relationship between test anxiety and personality, self-esteem in grade one senior high students.
Jin-tong LIU ; Xian-peng MENG ; Qing-zhi XU
Chinese Journal of Preventive Medicine 2006;40(1):50-52
OBJECTIVETo explore the relationship between test anxiety and personality, self-esteem in grade one senior high school students.
METHODSTotally 538 senior high school students of grade one were investigated by Test Anxiety Scale (TAS), Eysenck Personality Questionnaire (EPQ) and Self-Esteem Scale (SES) in a Senior High School in Shandong Province.
RESULTSThe prevalence of test anxiety among all the surveyed students was rated 65.2%. The Psychoticism (P) (51.60 +/- 9.66) or Neuroticism (N) (51.57 +/- 10.75) factor score of EPQ in students with test anxiety was significantly higher than that in students without test anxiety (48.07 +/- 8.62, 45.65 +/- 10.14) (P < 0.001), while the Extroversion or Introversion (E) score (50.76 +/- 11.09) was on the contrary (53.68 +/- 11.60) (P < 0.01). The total score of TAS was significantly positively related to the P (r = 0.14) and N (r = 0.36) factor score and significantly negatively related to the E factor score of EPQ (r = -0.15) (P < 0.001). The prevalence of test anxiety in introversive students (72.3%) was higher than that in extroversive students (53.2%) (P < 0.05), and that in students with unstable emotion (81.4%) and in students with apparent psychoticism (84.1%) were also higher than that in those with stable emotion (41.0%) and in those without psychoticism (57.7%) (P < 0.01). The total score of SES in students with test anxiety (29.12 +/- 4.41) was significantly lower than that in students without test anxiety (30.29 +/- 4.25) (P < 0.01). The total score of TAS was significantly negatively related to the total score of SES (r = -0.23) (P < 0.001).
CONCLUSIONTest anxiety should be related to the personality and self-esteem, and the prevalence of test anxiety in introversive, unstable emotional, apparent psychoticism or low self-esteem students should be higher.
Adolescent ; Anxiety ; psychology ; China ; Educational Measurement ; Female ; Humans ; Individuation ; Male ; Personality ; Self Concept ; Students ; psychology ; Test Anxiety Scale ; statistics & numerical data
9.Detection of weak D antigen by flow cytometry.
Xiao-Ying WU ; Hong-Xian XU ; Wen XIONG ; Chao-Peng SHAO
Journal of Experimental Hematology 2013;21(2):474-477
Flow cytometry was previously applied for analysis of Rh(D) antigen density, therefore it was suggested that the flow cytometry may be used for routine detection of weak D positive phenotypes. This study was purposed to evaluate its practicability. Six weak D positive and 7 DEL individuals were detected by using saline, IAT and absorption/elution test from 2010 to 2011 years. By RHD genotyping, zygosity analysis and sequencing, 3 cases of weak D type 15, 3 cases of partial D type DVI-III and 7 cases of DEL carrying RHD1227A alleles were identified. Taking 2 normal Rh(D)-positive and 2 D-negative samples as controls, all the samples were tested by using flow cytometry, and the median fluorescence intensities were observed as well. The results indicated that all weak D type 15 and partial D type DVI samples were detected to be positive by flow cytometry, as compared with 2 Rh(D)-negative samples (P < 0.05). Seven 7 DEL samples were tested to be negative (P > 0.05), although one of 7 DEL was tested as "±" in IAT and strong positive in absorption/elution. The RHD zygosity analysis showed this DEL individual as RHD(+)/RHD(+) homozygote. It is concluded that the sensitivity of detecting D antigen by flow cytometry is similar to that of IAT, but lower than absorption/elution test. As for detecting weak D or partial D antigens, IAT is easier than flow cytometry; as for identifying DEL, the flow cytometry is not sensitive enough.
Adult
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Alleles
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Blood Donors
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Blood Grouping and Crossmatching
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Flow Cytometry
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methods
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Genotype
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Humans
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Phenotype
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Rh-Hr Blood-Group System
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blood
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genetics
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immunology
10.Mechanism of anti-CXCR4 nanobody inhibiting angiogenesis in pancreatic cancer
Ya-xian LI ; Shu-yi XU ; Yue-jiang ZHENG ; Li-yun PENG ; Jian-wei ZHU ; Ming-yuan WU
Acta Pharmaceutica Sinica 2022;57(11):3331-3338
Tumor