Objective To establish and test the reliability and validity of the disease related psychological pressure scale of pregnant women with chronic HBV infection,so as to provide mental stress assessment tool for the pregnant women.Methods Item pool was developed after the interview and comprehensively retrieved literatures,then after expert review and rebuilt,the scale items were fixed.Totals of 368 pregnant women with chronic HBV infection disease were investigated and the reliability and validity of scale were test.Results The scale had 5 factors and 24 items,and the correlation coefficient between each factor score and total score was 0.712 - 0.894.The correlation coefficient among each factor was 0.409 - 0.631 which was significantly lower than that between each factor score and total score ( P ＜ 0.01 ).The cumulative contribution rate of 5 factors was 64.055％,and tes-retest reliability of 5 factors was 0.856,0.887,0.828,0.813.The Cronbach' s α of internal consistency of 5 factors was 0.788 - 0.865,and the Cronbach' s α of scale was 0.932.Conclusions The scale has good reliability and validity,and can be used for measuring the disease related pregnancy psychological pressure for chronic HBV infection pregnant women.

Adolescent ; Adult ; Aged ; Bronchi ; Child ; Female ; Foreign Bodies ; surgery ; Humans ; Male ; Middle Aged ; Surgical Mesh ; Trachea ; Young Adult

Adult ; Atypical Hemolytic Uremic Syndrome ; etiology ; Female ; Glomerulonephritis, IGA ; complications ; Humans ; Puerperal Disorders ; etiology

The megakaryocyte and platelet lineage may be one of the major sites of human cytomegalovirus (HCMV) infection. However, whether HCMV aggravates apoptosis in normal megakaryocytes was not well investigated. Megakaryocytic cell line CHRF-288-11 and HCMV AD 169 strain were co-cultured in this study. PCR was used to detect the direct infection of the cells by HCMV IEA expression. The apoptotic cells were analyzed by morphologic observation, DNA ladder formation, annexin V/PI and PI assay with flow cytometry. The results showed that HCMV significantly inhibited the growth of CHRF cells in three different concentrations of viral infection groups (10(-3), 10(-2), 10(-1)). The viability levels in each infection groups were 77%, 73% and 68% respectively after incubation for 7 days, compared with 98% in the control group. Using annexin V/PI with flow cytometry, it was shown that the percentages of apoptotic cells viral infection in groups (10(-3), 10(-2), 10(-1)) were (21.3 +/- 2.49)%, (25.8 +/- 3.65)% and (31.4 +/- 3.91)% at 7 days after infection, while the control was (3.68 +/- 1.47)%. The apoptotic cells were further confirmed by morphologic observation and DNA ladder formation. Furthermore, PCR detection also showed the direct infection by identification of HCMV IEA expression in CHRF cells. This study suggested that HCMV could directly infect megakaryocytes and aggravated apoptosis in HCMV-infected megakaryocytes.
Apoptosis ; Cell Survival ; Cells, Cultured ; Cytomegalovirus ; pathogenicity ; DNA, Viral ; analysis ; Humans ; Megakaryocytes ; cytology ; virology ; Polymerase Chain Reaction

Objective To analyze the relation between ~(99)Tc~m-DTPA renal dynamic imaging and pathological changes in patients with lupus nephritis (LN).Methods Ten normal control and 29 patients with LN underwent ~(99)Tc~m-DTPA renal dynamic imaging.The LN patients were divided into two groups:silent LN (SLN) group,18 patients;and obvious LN (OLN) group,11 patients.For each case,glomerular filtration rate (GFR),peak time (t_p),half excretion time (t_(1/2)) and the excretion rate at 20 min (R_(20)) were calculated.Assessment of renal function on the scintigraphic images was evaluated by nuclear medicine physicians.The t-test,Fisher'exact probability and R×C association were used for data analysis.Results There were significant differences between normal people and two goups of LN in tp(t=5.3,9.3,both P＜0.05),t_(1/2)(t=6.9,12.0,both P＜0.05)and R_(20)(t=10.1,12.1,both P＜0.05).As to GFR,there was significant decrease in OLN patients(t=4.1,P＜0.05),but not in SLN patients(t=1.7,P＞0.05).Diagnoses of renal function by renal dynamic imaging were compared with the renal pathological changes (r=0.2273,P＜0.05).Conclusions ~(99)Tc~m-DTPA renal dynamic imaging is useful for evaluation of the early stage renal function for LN patients and to diagnose LN patients with no symptom of renal impairment.It may help to assess the degree of renal parenchymal damage while obviating the need for renal biopsy in these patients.

Objective To discuss the risk factors of invasive fungal infection in preterm infants and conduct corresponding nursing intervention.Methods Retrospective analysis was used to investigate 1 146 preterm infants in Neonatal Intensive Care Unit (NICU) of the risk factors of invasive fungal infection.Results There were 66 preterm infants who had invasive fungal infection,with the rate of 5.76％,and it happened in (15.9 ± 4.8) days during hospitalization.Preterm infants with different gestational age,birth weight,time for intravenous nutrition,hospitalization period and mother having complication,asphyxiation,mechanical ventilation,parenteral nutrition,broad spectrum antibiotics or not had different rates of invasive fungal infection (P ＜ 0.05).Multivariate Logistic regression analysis showed that low birth weight,mechanical ventilation,broad spectrum antibiotics and intravenous nutrition for more than one week were the individual risk factors of invasive fungal infection in preterm infants (P ＜ 0.05).Conclusions Risk factors of invasive fungal infection in preterm infants include low birth weight,mechanical ventilation,broad spectrum antibiotics and intravenous nutrition for more than one week.Corresponding intervention methods should be taken to reduce the rate of invasive fungal infections.

Adult ; Antibodies ; blood ; Antiviral Agents ; therapeutic use ; DNA, Viral ; blood ; Female ; Hepatitis B Surface Antigens ; blood ; Hepatitis B e Antigens ; blood ; Hepatitis B virus ; drug effects ; Hepatitis B, Chronic ; blood ; drug therapy ; virology ; Humans ; Interferons ; immunology ; therapeutic use ; Liver Function Tests ; Male ; Middle Aged ; Young Adult

Objective： The circadian rhythm of DNA synthesis in nasopharyngeal carcinoma (NPC) cells was investigated so that to provide necessary data for making clinical chrono-chemotherapy schedule of NPC. Methods： Fifteen BALB/c mice were synchronized with an alternative lighting regimen with 12 hours for light and 12 hours for dark (12∶ 12 LD) at least 3 weeks. Human nasopharyngeal poorly differentiated squamous cell carcinoma(CNE-2) cells were implanted into both flanks of each mouse. Ten days after transplantation, sampling from tumor was conducted in the 3, 9, 15, 21 hours after light onset (HALO). Single cell suspension was obtained and stained with propidium iodide. The cellular DNA content was measured by flow cytometry. Data were documented by ANOVA and Cosinor analysis. Results： The proportion of tumor cells in Phage G1,S,G2-M varied according to circadian time with statistical significance. The distribution curves of Phage G1 and Phage G2-M fit for Cosinor changes. Conclusion： DNA synthesis of human nasopharyngeal poorly differentiated squamous cell carcinoma(CNE-2) cells trausplanted in nude mice varies according to circadian rhythm.

OBJECTIVETo investigate the effects of Xiezhuo Chubi Decoction (XZCBD) on the microRNA expression patterns of kidney in mice with hyperuricemia.

METHODSSixty Kunming male mice were randomly divided into the high-, medium-, and low-dose XZCBD groups, benzbromarone group, model group, and control group. Except the control group, all mice were established with yeast method combined with uricase inhibition method to build hyperuricemia model, and the corresponding drugs (37.5 g/kg, 18.75 g/kg, 9.375 g/kg, and 0.02 g/kg per day) were administrated on the 7th day. On the 22nd day, the blood uric acid concentration was detected, and microRNA with obvious changes in kidney was screened with qRT-PCR.

RESULTSThe uric acid in the model group was higher than that in the control group, and the levels of the uric acid were reduced after being treated with XZCBD; the differences among groups were significant (P<0.05). Compared with the control group, 32 kinds of microRNA expression changes were detected on the 15th day after being treated with high-dose XZCBD by microRNA expression profile screening. Among them, miR-34a could inhibit the expression of human urate anion exthanger 1, and miR-146a might have inhibited the inflammatory reaction.

CONCLUSIONXZCBD could significantly reduce the serum uric acid level; its effect on hyperuricemia might be through affecting microRNA expressions.

Animals ; Base Sequence ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Gene Expression Profiling ; Gene Expression Regulation ; drug effects ; Hyperuricemia ; blood ; drug therapy ; genetics ; Kidney ; drug effects ; metabolism ; pathology ; Male ; Mice ; MicroRNAs ; genetics ; Molecular Sequence Data ; Organic Anion Transporters ; genetics ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Uric Acid ; blood

OBJECTIVETo construct cDNA subtracted libraries from gastric dysplasia and further screen differentially expressed genes.

METHODSRelatively pure dysplasia and normal tissue were procured by manual microdissection, and amplified by cDNA-PCR, which was used to carry on for suppression subtractive hybridization (SSH). Subtracted cDNA fragments were linked with vector, cloned, screened, sequenced, and made homologous search. Differentially expressed fragments were verified by dot hybridization.

RESULTSTwo subtracted cDNA libraries were constructed. Among 26 sequenced clones, 15 fragments corresponded to known genes, 3 fragments were known EST and 8 fragments were unknown EST (GenBank BQ164614-BQ164616, BQ291516-BQ291520). Fifteen fragments were verified to be differentially expressed in gastric dysplasia.

CONCLUSIONSSubtracted cDNA libraries from gastric dysplasia are constructed using combination of microdissection-cDNA PCR and SSH setup in our laboratory. Some fragments have been screened and verified to help to search for novel associated genes with gastric carcinogenesis.

Cloning, Molecular ; DNA, Complementary ; genetics ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Gene Library ; Humans ; Microdissection ; Nucleic Acid Hybridization ; methods ; Polymerase Chain Reaction ; Precancerous Conditions ; genetics ; pathology ; Sequence Analysis, DNA ; Stomach Neoplasms ; genetics ; pathology