Adolescent ; Adult ; Brain Neoplasms ; diagnosis ; metabolism ; pathology ; surgery ; Female ; Follow-Up Studies ; Humans ; Magnetic Resonance Imaging ; Male ; Meningeal Neoplasms ; diagnosis ; metabolism ; pathology ; surgery ; Meningioma ; diagnosis ; metabolism ; pathology ; surgery ; Middle Aged ; Mucin-1 ; metabolism ; Neoplasm Recurrence, Local ; Reoperation ; Retrospective Studies ; Tomography, X-Ray Computed ; Vimentin ; metabolism ; Young Adult

DNA, Ribosomal ; genetics ; Genome, Plant ; Phylogeny ; Polymerase Chain Reaction ; methods ; Polymorphism, Single Nucleotide ; genetics ; RNA, Ribosomal, 5S ; genetics ; Sequence Homology, Nucleic Acid ; Species Specificity ; Valerian ; classification ; genetics

OBJECTIVETo establish a quantitative method for determination of synephrine and N-methyltyramine in Citri Reticulatae.

METHODSamples were extracted with 30% methanol. ODS column was used with methanol-water-sodium dodecyl sulfate (55:45:0.1) as mobile phase. Detection wavelength was 285 nm.

RESULTSynephrine and N-methyltyramine in sample solution were well separated. Linearity of synephrine was good (r = 0.9999) in range of 0.35-11.24 microg. The average recovery was 97.1%, and RSD of repeatability was 1.9%.

CONCLUSIONThis method can be used for quality control of Citri Reticulatae.

Chromatography, High Pressure Liquid ; Citrus ; chemistry ; Fruit ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control ; Seasons ; Synephrine ; analysis ; Tyramine ; analogs & derivatives ; analysis

OBJECTIVETo establish the quality standard of yinhuang langxiao granule.

METHODThe Panax notoginseng, Arnebia euchromq, Cornus officinalis, Lonicera japonica, Artemisiae annua were identified by TLC. The content of chrysophanol in granule was determined by HPLC.

RESULTSpots of samples on TLC can be well separated and the method had good specificity. The average recovery of chrysophanol was 98.7% and RSD was 0.67%.

CONCLUSIONAn accurate, simple and effective quality-controlling method has been developed, which can be used for quality control for yinhuang langxiao granule.

Anthraquinones ; analysis ; Artemisia ; chemistry ; Boraginaceae ; chemistry ; Chromatography, High Pressure Liquid ; Chromatography, Thin Layer ; Cornus ; chemistry ; Drug Combinations ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Lonicera ; chemistry ; Panax ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control ; Rheum ; chemistry

Objective To evaluate the value of transesophageal echocardiography (TEE) in transthoracic minimally invasive device closure of ventricular septal defect(VSD).Methods A total of 164 cases of VSD were recruited as candidates to receive transthoracic minimally invasive device closure between January 2007 and October 2010,including 138 perimembranous VSDs,3 muscular VSDs and 23 supracristal VSDs.Among these groups,85 male patients were included.Four-champer view,five-champer view,left ventricular long-axis view,short-axis view and right ventricular inflow view were detected to evaluate the availability of device closure.Results A total of 152 cases(92.7%)were successfully closed with a device.All the patients were followed up more than 3 months arranged with a standard protocol.No complete atrioventricular block (CAVB) or associated valvular complications were observed.Three of the five cases with traced residual shunt after device closure closed spontaneously.Conclusion TEE plays an important role in transthoracic minimally invasive device closure of ventricular septal defect,which has been proved by the good follow-up results without CAVB and associated valvular complications.

Objective To investigate the molecular epidemiologic characteristics and genotypes of norovirus in children less than 5 years of age in Lulong area from 2008 to 2009.Methods 325 stool specimens and epidemiological data from hospitalized children with diarrhea less than 5 years of age were collected.Rotavirus was detected by using the ELISA kit.Norovirus,adenovirus and astrovirus were detected by multiple reverse transcription-polymerase chain reaction (RT-PCR).Partial norovirus strains were sequenced and the tree was conducted by using the phylogenetic analyses.Results Norovirus was detected in 37out of 325 (11.3％ ) specimens,ranked only second to rotavirus (48.6％),and higher than adenovirus (6.5 ％ ) and astrovirus (4.3％).Norovirus predominantly infected children less than 2 years of age and the season peak of norovirus occurred in November.Phylogenetic analysis showed that the predominant strain was the GⅡ.4/2006b variant.Interestingly,a novel unreported GⅡ-4 variant was found in this study.Conclusion Norovirus was one of the most important pathogens causing acute gastroenteritis from 2008 to 2009 in Lulong area.The GⅡ.4/2006b vairant was still the predominant strain.It is important to keep on monitoring the novel GⅡ.4 variant.

Objective To screen differentially expressed miRNAs(DEMs)by comparing the expression of miRNAs in serum exosomes between Alzheimer's disease(AD)patients and healthy controls.Methods A total of 71 AD patients admitted to Department of Geriatric Neurology of Xiangya Hospital from March 2017 to August 2018 and another 71 healthy individuals who taking physical examination in the hospital during same period were recruited and assigned into AD and HC groups,respectively.Four AD patients and four healthy subjects were selected for high-throughput second-generation sequencing of exosome miRNAs.The results were analyzed to obtain the DEMs between them,and the top 4 DEMs were finally selected.Then real-time quantitative real-time PCR was applied for all the subjects to detect the expression of the 4 DEMs.Results High-throughput second-generation sequencing detected 775 miRNAs,and 44 DEMs were found with statistical difference between the 2 groups(P＜0.05).Compared with the HC group,34 miRNAs were up-regulated and 10 were down-regulated in the AD group.The top 4 DEMs were miRNA-148a-3p,miRNA-16-5p,miRNA-19b-3p and miRNA-483-5p.MiRNA-148a-3p was significantly up-regulated in the AD group than the HC group(P＜0.01),but there were no significant differences in the expression level in the other 3 DEMs between the 2 groups.ROC curve analysis showed that the area under the curve of miRNA-148a-3p was 0.7113(95％CI:0.622～0.801),with a sensitivity of 71.6％and a specificity of 69.7％.Conclusion Serum exosome miRNA-148a-3p can be used as a biomarker for the diagnosis of AD.

OBJECTIVETo investigate the prevalence of human papillomavirus (HPV) and the HPV genotype distribution in invasive squamous cell carcinoma of the uterine cervix in the Mongolian women in Inner Mongolia autonomy region.

METHODSThe prevalence data of HPV in our department were retrospectively reviewed. INNO-LiPA genotyping technique was used to detect HPV genotypes in the reserved carcinoma tissue specimens.

RESULTSTotally 63 tissue specimens were collected and detected. The prevalence of HPV was 93.7%. The positive rates of HPV among different clinical staging and different pathological grading were not significantly different (P >0.05). The prevalence of HPV16 was not significantly different among different age groups (P>0.05). HPV16 (69.8%), HPV18 (4.8%), HPV31 (4.8%), HPV39 (4.8%), and HPV52 (3.2%) were the 5 dominating HPV genotypes in all cases.

CONCLUSIONSHPV infection is closely correlated with invasive squamous cell carcinoma of the uterine cervix in Mongolia women. HPV16 is the most important genotype in invasive squamous cell carcinoma of the uterine cervix, followed by HPV18, 31, and 39. HPV infection dose not affect the progression and differentiation of invasive squamous cell carcinoma of the uterine cervix.

Adult ; Aged ; Asian Continental Ancestry Group ; Carcinoma, Squamous Cell ; virology ; Female ; Genotype ; Humans ; In Vitro Techniques ; Middle Aged ; Papillomaviridae ; classification ; genetics ; Papillomavirus Infections ; genetics ; virology ; Polymerase Chain Reaction ; Uterine Cervical Neoplasms ; virology

To analyze the gE gene sequence of varicella-zoster virus (VZV) strains of different clades and subclades currently circulating in China. Eighteen skin lesion fluid swabs or skin scab pieces from patients with chickenpox or shingles were obtained from Beijing, Changchun, Lhasa and Urumqi between December 2010 and June 2011. The genotype of the virus strains was determined by a group of single nucleotide polymorphism (SNP) located in 15 ORFs, and the full-length gE genes of 18 strains representing all the clades in the study was amplified by PCR and sequenced. In addition to the synonymous mutations and non-synonymous mutations that were reported in the literature, there were 3 novel non-synonymous mutations (C56T, C1109T, C917A) and 4 new synonymous mutations (C54T, T1075C, T816C, G279A) found in the 8 strains analyzed. We found the VZV strains of clade 5 in Xinjiang for the first time,and the genotypes of some VZV strains circulating in Chagnchun could not be determined by the present methods. The analysis of gE gene sequences,revealed a novel non-synonymous mutations in the e1 and c1 epitopes, corresponding to the amino acid change of serine to tyrosine.
Adolescent ; Adult ; Aged ; Base Sequence ; Chickenpox ; virology ; Child ; China ; Female ; Genotype ; Herpesvirus 3, Human ; classification ; genetics ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; Mutation, Missense ; Open Reading Frames ; Sequence Analysis, DNA ; Viral Envelope Proteins ; genetics ; Viral Proteins ; genetics ; Young Adult

Exosomes are spherical microvesicles(30-100 nm)secreted by a variety of cells and contain a variety of proteins, lipids, mRNAs, and microRNAs.These molecules into exosomes can be transferred to adjacent cells and distant tissues or cells by exosomes to develop full effects.Exosomes-released microRNAs are involved in the pathogenesis of Alzheimer's disease(AD)by regulating the abnormal expression of amyloid β-protein(Aβ)and p-Tau protein, initiating the inflammatory response by interacting with toll-like receptors.In addition, exosomal microRNAs can be used as a potential therapeutic target for AD.Exosomes as good carriers have great research values.This review summarized the literatures related to the role of exosomal microRNAs in the development of AD and the potential therapeutic effect on AD.A large amount of evidence indicates that expression disorder of exosomal microRNA plays an important role in the pathogenesis of AD and can be used as a new potential biomarker and therapeutic target for the diagnosis and treatment of AD.