1.Normal sperm morphology and the outcomes of routine in vitro fertilization.
Bing HE ; Jun-ping CHENG ; Qi PAN ; Yan CHI ; Tai-shuai HUANG ; Xian-bao MAO ; Jie QIN ; Wei-hong TAN
National Journal of Andrology 2016;22(1):32-36
OBJECTIVETo explore the application value of morphology assessment of sperm from fresh semen in routine in vitro fertilization (IVF).
METHODSWe analyzed the morphology of the sperm from fresh or optimized semen samples and, based on the sperm morphology of the raw semen, allocated 908 IVF cycles due to the pure tubal factor to different groups: morphologically normal sperm (MNS) ≤ 4%, > 4% - ≤ 15%, and > 15% in Trial 1 and MNS ≤ 1%, > 1% - ≤ 2%, > 2% - ≤ 3%, and > 3%-- ≤ 4% in Trial 2. We compared the rates of fertilization, cleavage, high-quality embryo, -blastocyst formation, and pregnancy among different groups.
RESULTSThe total fertilization rate was significantly lower in the MNS ≤ 4% than in the MNS > 4% - ≤ 15% and >15% groups (74.40% vs 78.61% and 80.03%, P < 0.01). Compared with the MNS ≤ 1%, > 1% - ≤ 2%, and > 2% - ≤ 3% groups, the MNS > 3% - ≤ 4% group showed remarkably increased rates of 2PN normal fertilization (77.23%, 78.97% and 78.99% vs 85.47%, P < 0.01), cleavage (95.71%, 96.01% and 97.27% vs 98.73%, P < 0.05), and blastocyst formation (53.85%, 49.01% and 49.55% vs 63.41%, P < 0.01). No statistically significant differences were observed in the rates of clinical pregnancy, implantation, early abortion, live birth, or malformation at birth among different groups (P > 0.05).
CONCLUSIONMNS ≤ 4% affected the total rate of fertilization while MNS ≤ 3% reduced the rate of normal fertilization in IVF. However, even MNS ≤ 1% did not result in fertilization disorder or failure. Therefore, teratozoospermia alone was not an indicator of ICSI and sperm mor- phology assessment had no obvious value for predicting the rates of embryo quality, clinical pregnancy, and live birth in IVF.
Female ; Fertilization in Vitro ; Humans ; Male ; Pregnancy ; Pregnancy Outcome ; Spermatozoa ; cytology
2.Simultaneous Determination of Eight Saponins in Gynostemma pentaphyllum(Thunb.) Makino by HPLC
Hui-Xuan JIANG ; Can-Wen CHEN ; Mao-Bao XU ; Xian-Tao ZHANG
Journal of Guangzhou University of Traditional Chinese Medicine 2018;35(2):324-328
Objective To establish a high performance liquid chromatography(HPLC) method for the simultaneous determination of eight components in Gynostemma pentaphyllum(Thunb.) Makino (ginsenosides Rb1, Rb3, Rd, Re, F2, and gypenosides A, XLIX and XVII). Methods HPLC was performed on Agilent Eclipse XDB-C18(4.6 mm × 250 mm, 5 μm) chromatographic column with 0.3% formic acid solution (A)- acetonitrile (B) as the mobile phase by gradient elution, flow rate was 1.5 mL·min-1, detection wavelength was 203 nm, and column temperature was set at 50 ℃. Results There was a good linear relationship between peak area and concentration of eight components (r ≥ 0.999) , the recovery was in the range of 97% to 100%. Conclusion The established method is simple, rapid, accurate, reliable, and reproducible, and can be used for the quality control of Gynostemma pentaphyllum(Thunb.) Makino.
3.Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified Gerbera.
Qi-xian LAI ; Zhi-yi BAO ; Zhu-jun ZHU ; Qiong-qiu QIAN ; Bi-zeng MAO
Journal of Zhejiang University. Science. B 2007;8(7):458-464
Leaf senescence is often caused by water deficit and the chimeric gene P(SAG12)-IPT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance (TBARS) and antioxidant enzymes activities were investigated during leaf senescence of P(SAGl2)-IPT modified gerbera induced by osmotic stress compared with the control plant (wild type). Leaf discs were incubated in 20%, 40% (w/v) polyethylene glycol (PEG) 6000 nutrient solution for 20 h under continuous light [130 micromol/(m(2) x s)]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase (SOD), catalases (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and dehydroascorbate reductase (DHAR) were stimulated by PEG treatment. However, the increases were higher in P(SAG12)-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation (TBARS content) was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities of antioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress.
Alkyl and Aryl Transferases
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genetics
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Antioxidants
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metabolism
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Arabidopsis Proteins
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genetics
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Ascorbate Peroxidases
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Asteraceae
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genetics
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metabolism
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Carotenoids
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metabolism
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Catalase
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metabolism
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Chlorophyll
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metabolism
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Cysteine Endopeptidases
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genetics
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Genes, Bacterial
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Genes, Plant
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Lipid Peroxidation
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Osmotic Pressure
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Oxidoreductases
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metabolism
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Peroxidase
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metabolism
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Peroxidases
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metabolism
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Plant Leaves
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metabolism
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Plant Proteins
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metabolism
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Plants, Genetically Modified
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Promoter Regions, Genetic
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Solubility
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Superoxide Dismutase
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metabolism
4.Effect of interferon neutralization antibody in the treatment of chronic hepatitis B with interferon.
Xian-rui CHEN ; Hui-liang WANG ; Mei-xiang XUAN ; Xin-gang MAO ; Jian-guo SUN ; Bao-mei WAN
Chinese Journal of Hepatology 2009;17(5):385-386
Adult
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Antibodies
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blood
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Antiviral Agents
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therapeutic use
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DNA, Viral
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blood
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Female
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Hepatitis B Surface Antigens
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blood
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Hepatitis B e Antigens
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blood
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Hepatitis B virus
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drug effects
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Hepatitis B, Chronic
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blood
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drug therapy
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virology
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Humans
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Interferons
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immunology
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therapeutic use
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Liver Function Tests
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Male
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Middle Aged
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Young Adult
5.A method to avoid the fixator failure by using pedicle screw combined vertebroplasty for spine fractures.
Sheng TAO ; Ke-ya MAO ; Bao-wei LIU ; Yan WANG ; Yu-tian LIANG ; Pei-fu TANG ; Hui-xian WANG
Chinese Journal of Surgery 2006;44(16):1098-1100
OBJECTIVETo study a new implant material (carbonated hydroxyapatite, CHA) united pedicle screw to cure spine fracture.
METHODSThirty-two cases of spine compressed fracture were used with pedicle screw fixator and vertebroplasty. Before operation, patients' vertebral body were compressed (46 + 21)% (20% approximately 70%) on average. In operation, broken vertebral body was reposition through pedicle screw technique, then used self-made syringe to inject CHA into anterior and central column of broken vertebral body through pedicle. And all of patients were not given any bone-graft.
RESULTSIn 6 - 26 months followed-up, no immunologic rejection was found about hydroxyapatite, and no any broken of the screws and shafts was found, no loosing and other complications either. All the patients could move in 3 - 5 days after operation. The height of the broken vertebral body were reduced 97% compared with pre-operation. And CHA in vertebral body was degraded gradually, and at the same time it was replace by new bone in vertebral body. After operation, VAS score was 61 +/- 32, and there was significant difference compared with pre-operation.
CONCLUSIONSThe pedicle screw fixation united vertebroplasty is an efficient way to prevent the failure of the treatment of spine fracture.
Adult ; Bone Screws ; Bone Substitutes ; therapeutic use ; Durapatite ; therapeutic use ; Female ; Follow-Up Studies ; Fracture Fixation, Internal ; instrumentation ; methods ; Fractures, Compression ; surgery ; Humans ; Male ; Middle Aged ; Spinal Fractures ; surgery ; Vertebroplasty ; methods
6.Hyperthermia enhanced the killing effect of 5-fluorocytosine on human colon cancer cell line transfected with cytosine deaminase gene.
Jin-mao LI ; Cheng-jin LI ; Da-nian LAI ; Xiao-jun WANG ; Xian-li HE ; Guo-qiang BAO ; Tao WU ; Ji-kai YIN
Chinese Journal of Gastrointestinal Surgery 2006;9(3):234-237
OBJECTIVETo investigate whether hyperthermia can enhance the killing effect of 5- fluorocytosine (5- FC) on human colorectal carcinoma cell lines SW480 transfected with carcinoembryonic antigen (CEA) tissue- specific cytosine deaminase (CD) gene in vitro,and study its mechanism.
METHODSHuman colorectal carcinoma cell lines SW480 transfected with G1CEACDNa were cultured. The proliferated colonies were treated with the combined therapy of 5-FC and hyperthermia at a temperature of 43 degrees C for 30 min. After eight days, MTT was used to calculate the cellular survival rate,to analyze the killing effect of 5-FC combined with hyperthermia on SW480 cells transfected with CD gene. Flow cytometry was performed to analyze the cellular cycle and transmission electron microscope was used to observe the morphologic changes of SW480 cells after thermochemotherapy.
RESULTSHyperthermia combined with 5-FC had an enhanced killing effect on SW480-CEACD cells than 5-FC alone (P< 0.05, t =2.403, n=9). Flow cytometry revealed that the proportion of S stage cell increased in the group treated with hyperthermia and 5- FC (P< 0.001, t =7.158, n=6). Transmission electron microscope showed apoptosis after thermo- chemotherapy.
CONCLUSIONSHyperthermia can improve the anti- tumor effect of 5- FC on human colorectal carcinoma cell lines SW480 transfected with CD gene, and the cells were blocked at S stage of cellular cycle and apoptosis was induced following thermochemotherapy.
Cell Line, Tumor ; Cytosine Deaminase ; genetics ; Flucytosine ; pharmacology ; Genes, Transgenic, Suicide ; Genetic Therapy ; methods ; Hot Temperature ; Humans
7.The different impact between late-phase of limb ischemia preconditioning and early-phase on patients with percutaneous coronary intervention
rong Jia LIANG ; qiu Liang TANG ; xian Yun CHEN ; bing She ZHANG ; mao Wen FAN ; feng Bao CHEN ; feng Jin CHEN ; ying Xiang LIU ; hui Jin HOU
Chinese Journal of Interventional Cardiology 2017;25(10):568-572
Objective To investigate the difference of late-phase of limb ischemia preconditioning (L-LIP) verse early-phase (E-LIP) on patients with percutaneous coronary intervention (PCI).Methods A total of 160 patients with unstable angina pectoris who were planned to undergo PCI were divided equally into two groups at random.The late-phase of limb ischemia preconditioning group (80 patients) were provided with L-LIP (three 5-minute inflations up to 200mmHg by applying the sphygmomanometer cuff around the right upper arm,followed by 5-min intervals of reperfusion,twice a day) 3 days before PCI.The Earlyphase of limb ischemia preconditioning group (80 patients) were provided with E-LIP (method as above)2 hours before PCI.Comparison of procedural parameters during PCI and the levels of cTnT,CK-MB,hs-CRP were made 24 hours after PCI.Estimation of the rate of adverse events at 1 year between the two groups was evaluated by Kaplan-Meier analysis.Results Compared to the E-LIP group,the rates of angina,arrhythmia and TIMI flow ≤ 2 during PCI were significantly lower in the L-LIP group (all P < 0.05).At 24 hours after PCI,the levels of cTnT and CK-MB were declined more significantly in the L-LIP group[(11.52±2.41) pg/ml vs.(27.53±4.78)pg/ml,P =0.021;(14.11±2.87)Iu/L vs.(30.23±5.17)Iu/L,P =0.032].There was no difference in the level of hs-CRP between the 2 groups [(128±0.71)mg/dl vs.(1.33±0.69)mg/dl,P =0.742].The Kaplan-Meier survival curve showed that the incidence rate of adverse events in the L-LIP group at l year was lower than the E-LIP group (3.75% vs.13.75%,P =0.024).Conclusions L-LIP is more effective to in protecting myocardial cell in patients with unstable angina pectoris undergoing elective PCI and may reduce the rate of future adverse event.
8.Retrospective cohort study on the rate of mother-to-child transmission among mothers infected with human immunodeficiency virus type 1 through blood transfusion
Su-Liang CHEN ; Hong-Ru ZHAO ; Xiao-Mei LI ; Yu-Qi ZHANG ; Guang-Sheng JIN ; Cui-Ying ZHAO ; Yan-Ping ZHANG ; Guang-Yi BAI ; Bao-Jun LI ; Liang LIANG ; Zhi-Qiang CHEN ; Yan-Liang HUI ; Fu-Bao LIU ; Zhi-Xian XU ; Jian-Guo ZHU ; Yu-Rong MAO
Chinese Journal of Epidemiology 2009;30(6):564-566
Objective To study the rate of mother-to-child transmission (MTCT) on HIV-1. Methods All local residents from 8 townships in a region were screened for mothers who had a history of only one blood transfusion and 63 were found HIV-1 positive. A further study on these HIV-1 positive mothers and their children was conducted with the emphasis on the date of receiving blood transfusion, date and type of nationality, history regarding breastfeeding and so on. Sera specimens from 84 children born from 63 HIV-1 positive mothers were screened, using ELISA for HIV-1 antibody, and positive specimens were confirmed by Western-blot. Results The rate of MTCT was 32.1% (27/84) for children with all risk factors related to MTCT. Another 36.8% (7/19) were related to factors on intrauterine, intrapartum and breastfeeding, 35.7% (5/14) to intrapartum and breastfeeding factors, 14.3% (2/14) to intrauterine and intrapartum factors, 37.9% (11/29) to breastfeeding factor alone. By group combination analysis, the MTCT rate was 36.9% (24/65) with breastfeeding, 11.8% (2/17) with artificial feeding, and the former was significantly higher than the latter. Conclusion HIV-1 MTCT rate among mothers caused by a single blood transfusion varied with different risk factors. Breastfeeding played an important role in MTCT, appeared in our study.
9.Capecitabine combined with cisplatin as first-line therapy in Chinese patients with advanced gastric carcinoma-a phase II clinical study.
Bing HU ; Ji-Ren YU ; Zhao-Zhang WEN ; Yong-Qian SHU ; Bao-Cheng WANG ; Hao-Ran YIN ; Li CHEN ; Yu-Xian BAI ; Jun LIANG ; Li CHEN ; Ying CHENG ; Lin SHEN ; Yun ZHOU ; Hong-Gang ZHANG ; Jie LI ; De-Sen WAN ; Shuang CHEN ; Ting-Zhen JIA ; Mao-Lin JIN
Chinese Journal of Oncology 2008;30(12):940-943
OBJECTIVETo evaluate the effectiveness and safety of the combination chemotherapy of capecitabine (X) with fractionated administration of cisplatin (C) in Chinese patients with advanced gastric cancer (AGC).
METHODS141 patients with AGC were enrolled between July 2002 and August 2004. All patients had measurable tumor according to the criteria of RECIST, Karnofsky performance status > or = 60, adequate bone marrow, renal and hepatic functions. Prior radiotherapy or adjuvant chemotherapy was not permitted. Patients received oral administration of capecitabine at a dose of 1000 mg/m(2) twice a day on D1-D14, and intravenous infusion of fractionated cisplatin at a dose of 20 mg/m(2)/day on D1-D5. The regimen was repeated every 3 weeks, totally for 6 cycles.
RESULTSOf the 141 evaluable patients, there were 104 men and 37 women, with a median age of 54 years (range, 23 - 80 years). Metastases before chemotherapy were detected in lymph nodes (46.8%), liver (40.4%), lung (5.7%) and other area (10.6%). The median treatment duration was 6 cycles (range, 3 - 6 cycles). The objective response rate (RR) was 36.2% (51/141). The median follow-up period was 17.5 months. The median time to progress (TTP) was 9.0 months, and the median overall survival (OS) was 12.0 months. The most common treatment-related adverse events (grade 3/4) were: hand-foot syndrome (HFS) (2.1%), leucopenia (0.7%), abnormal alanine transaminase elevation (2.8%). There was no treatment-related death.
CONCLUSIONCapecitabine combined with fractionated cisplatin is highly effective and well tolerated as a first-line treatment for advanced gastric cancer, with comparable results to 5-Fu plus cisplatin combination therapy.
Adult ; Aged ; Aged, 80 and over ; Antineoplastic Combined Chemotherapy Protocols ; adverse effects ; therapeutic use ; Capecitabine ; Cisplatin ; administration & dosage ; adverse effects ; Deoxycytidine ; administration & dosage ; adverse effects ; analogs & derivatives ; Female ; Fluorouracil ; administration & dosage ; adverse effects ; analogs & derivatives ; Follow-Up Studies ; Foot Dermatoses ; chemically induced ; Hand Dermatoses ; chemically induced ; Humans ; Leukopenia ; chemically induced ; Liver Neoplasms ; drug therapy ; secondary ; Lung Neoplasms ; drug therapy ; secondary ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Staging ; Remission Induction ; Stomach Neoplasms ; drug therapy ; pathology ; Survival Rate ; Vomiting ; chemically induced ; Young Adult
10.Chordoid meningioma: a retrospective study of 17 cases at a single institution.
Hong-da ZHU ; Hong CHEN ; Qing XIE ; Ye GONG ; Ying MAO ; Ping ZHONG ; Xiao-ming CHE ; Chen-chuan JIANG ; Feng-ping HUANG ; Kang ZHENG ; Shi-qi LI ; Yu-xiang GU ; Wei-ming BAO ; Bo-jie YANG ; Jin-song WU ; Yin WANG ; Li-qian XIE ; Ming-zhe ZHENG ; Hai-liang TANG ; Dai-jun WANG ; Xian-cheng CHEN ; Liang-fu ZHOU
Chinese Medical Journal 2013;126(4):789-791
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
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Meningioma
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diagnosis
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Middle Aged
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Retrospective Studies
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Young Adult