2.An experimental study of vascularization monitoring of tissue engineered bone in the rhesus
Xue-Ming WANG ; Guo-Xian PEI ; Dan JIN ; Kuanhai WEI ; Shan JIANG ; Guanghui TANG ;
Chinese Journal of Orthopaedic Trauma 2004;0(06):-
Objective To compare four methods of monitoring vascularization of tissue engineered bone in the rhesus so as to find our the best. Methods Twenty-five lower limbs of 13 rhesuses were used in this study to make models of tibial diaphyseal defect of 20mm which were to be fixed with an AO reconstruction plate of 7 holes. The monkeys were randomly divided into five groups according to defect filling materials: group A:?-tricalcium phosphate (?-TCP) and bone marrow stromal cells (BMSCs) and blood vessel bundles; group B:?-TCP and blood vessel bundles; group C:?-TCP and BMSCs; group D:?-TCP; group E: blank. Perfusion weighted MR imaging (PWMR), X-ray, radionuclide imaging and histological examinations were carried out at weeks 4, 8, 12 postop- eratively. The maximum slope rates of the single intensity-time curve (SS_(max)) and values of baseline (Sl_(?))were calculated at the same time points. Transmittances of the X-ray films were assessed. Ratios between isotope counts in region of interest (ROI) were calculated. Chinese ink perfusion and calculation of blood vessel areas were done for histological examinations, Results Compared with other groups, the SS_(max) in group A was the highest at weeks 4, 8, 12 postoperatively. In group A, the SS_(max) at week eight was significantly higher than that at week four (P= 0. 003), and the SS_(max) and transmittance of X-ray were negatively related at week 12 after operation (rs=-0. 892, P=0. 042), but the SS_(max) and blood vessel area were positively related (rs=0. 894, P=0.041)Conclusions PWMR can be a sensitive, quantitative, noninvasive and non-radiant method to monitor vascularization of tissue engineered bone, because SS_(max) of the single intensity-time curve of PWMR can reflect the most accurately the process of vascularization of tissue engineered bone.
3.Clinical study of Qinggan Huatan Huoxue Recipe on the treatment of non-alcoholic steatohepatitis.
Qiang PEI ; Xiao-Su WANG ; Xian-Bo WANG
Chinese Journal of Integrated Traditional and Western Medicine 2012;32(1):29-31
OBJECTIVETo observe the therapeutic effects of Qinggan Huatan Huoxue Recipe (QHHR) on patients with non-alcoholic steatohepatitis (NASH).
METHODSOne hundred and fifty NASH patients were randomly assigned to the treatment group (78 cases) and the control group (72 cases). QHHR was given to patients in the treatment groups, while Danning Tablet was given to those in the control group. The therapeutic course for all was three months. Before and after treatment changes of clinical symptoms and physical signs, liver imageology, liver functions, blood lipids, and insulin resistance index (IRI) were observed.
RESULTSCompared with before treatment, obvious improvement of clinical symptoms, weight, body mass index (BMI), liver functions, blood lipids, and integral of liver ultrasound B was obtained in the two groups (P < 0.05). The IRI of the treatment group was significantly reduced after treatment (P < 0.05). Better effects were obtained in lowering the body weight, BMI, alanine aminotransferase (ALT), gamma glutamyltransferase (gamma-GT), triglyceride (TG), total cholesterol (TC), integral of liver ultrasound B, and the total effective rate (P < 0.05).
CONCLUSIONSQHHR had definite effects on NASH. Its therapeutic effects were better than Danning Tablet.
Adolescent ; Adult ; Aged ; Drugs, Chinese Herbal ; therapeutic use ; Fatty Liver ; drug therapy ; Female ; Humans ; Male ; Middle Aged ; Non-alcoholic Fatty Liver Disease ; Phytotherapy ; Young Adult
4.The effect of hypoxia-early reoxygenation on persistent sodium current in single ventricular myocytes of guinea pig.
Xian-Pei WANG ; Ji-Hua MA ; Pei-Hua ZHANG ; Jun LI
Chinese Journal of Applied Physiology 2006;22(1):16-20
AIMTo investigate the effect of hypoxia/early reoxygenation on persistent sodium current (I(Na.P)) in single ventricular myocytes of guinea pig and discuss its role and significance during this pathological condition.
METHODSThe whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia/reoxygenation model.
RESULTS(1) With 0.5 Hz, 1 Hz and 2 Hz pulse frequency, the current density gap between the first and the eighth pulse of I(Na.P) was (0.021 +/- 0.014) pA/ pF, (0.097 +/- 0.014) pA/pF and (0.133 +/- 0.024) pA/pF (P < 0.01) respectively. (2) Depolarization with membrane holding potential of -150 - -80 mV respectively, I(Na.P) density attenuated gradually. (3) The amplitude of I(Na.P) was increased consistently with the prolongation of hypoxia time during hypoxia. (4) I(Na.P) was (0.500 +/- 0.125) pA/pF, (1.294 +/- 0.321) pA/pF and (0.988 +/- 0.189) pA/pF (P < 0.01, vs normoxia, respectively) during normoxia, hypoxia after 15 min and reoxygenation after 5 min, respectively.
CONCLUSIONThese results indicate that I(Na.P) has great significance in arrhythmogenesis and calcium-overload, which causes the following postischemia and post hypoxia myocardial damage.
Animals ; Calcium ; metabolism ; Cell Hypoxia ; Guinea Pigs ; Heart Ventricles ; Membrane Potentials ; Myocytes, Cardiac ; metabolism ; physiology ; Oxygen ; metabolism ; Patch-Clamp Techniques ; Sodium ; metabolism ; Sodium Channels ; metabolism
5.Nitric oxide increases persistent sodium current of ventricular myocytes in guinea pig during normoxia and hypoxia.
Ji-Hua MA ; Xian-Pei WANG ; Pei-Hua ZHANG
Acta Physiologica Sinica 2004;56(5):603-608
Whole cell patch-clamp technique was used to record the changes of persistent sodium current (I(Na.P)) and the effect of administered agents in ventricular myocytes of guinea pig to investigate the essence of I(Na.P) and mechanism of increased I(Na.P) of ventricular myocytes during hypoxia. The results showed: (1) Pro-NO L-arginine(L-Arg) and donor sodium nitroprusside (SNP) increased I(Na.P) in a concentration-dependent manner in normoxia. (2) I(Na.P) increased gradually with the prolongation of hypoxia time. After 15 min of hypoxia, administration of N(G)-nitro-L-arginine methyl ester (L-NAME), a NO synthase inhibitor, could not significantly recover the increased I(Na.P) [(1.344+/-0.320) vs (1.301+/-0.317) pA/pF, P>0.05, n=5]; (3) During hypoxia the perfusion solution with L-NAME decreased the increased I(Na.P), and the difference was significant compared with pure hypoxia [(0.914+/-0.263), n=5 vs (1.344+/-0.320) pA/pF, P<0.05, n=6], whereas the amplitude of I(Na.P) was still larger than that in normoxia [(0.914+/-0.263) vs (0.497+/-0.149) pA/pF, P<0.05, n=5]; (4) Reducing agent dithiothreitiol (DTT) not only recovered the increased I(Na.P) by L-Arg and administered SNP after hypoxia [(1.449+/-0.522) vs (0.414+/-0.067) pA/pF, P<0.01, n=6, and (1.786+/-0.636) vs (0.436+/-0.141) pA/pF, P<0.01, n=5, respectively], but also decreased the I(Na.P) in normoxia [(0.442+/-0.056) vs (0.396+/-0.057) pA/pF, P<0.01, n=6]. Our results suggest that hypoxia increases I(Na.P) of ventricular myocytes, which is induced by raised NO oxidating sodium channel protein in myocardial membrane during hypoxia. The activity of I(Na.P) in normoxia is related to the oxidation state of the channel protein.
Animals
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Cell Hypoxia
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physiology
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Cell Separation
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Female
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Guinea Pigs
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Heart Ventricles
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Male
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Myocardium
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cytology
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Myocytes, Cardiac
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metabolism
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physiology
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Nitric Oxide
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pharmacology
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Nitroprusside
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pharmacology
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Patch-Clamp Techniques
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Sodium Channels
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physiology
6.Effects of telmisartan on voltage-gated Kv1.3 and Kv1.5 potassium channels expressed in Xenopus oocytes.
Mu-Wei LI ; Xian-Pei WANG ; Chuan-Yu GAO ; An-Ruo ZOU
Chinese Journal of Cardiology 2009;37(2):165-168
OBJECTIVETo observe the effects of telmisartan on Kv1.3 and Kv1.5 potassium channels expressed in Xenopus oocytes.
METHODSKv1.3 and Kv1.5 potassium channel currents expressed in Xenopus oocytes were recorded and observed in the absence and presence of telmisartan using standard two-microelectrode voltage clamp techniques.
RESULTSTelmisartan resulted in a concentration- and voltage-dependent inhibition effect on Kv1.3 channel current (IC(50) 2.05 micromol/L)and on Kv1.5 channel current (IC(50) 2.37 micromol/L).
CONCLUSIONSTelmisartan blocks open-state Kv1.3 channel which could be one of the mechanisms related to its immunomodulatory and anti-atherosclerosis effect. Telmisartan also blocks open-state Kv1.5 channel which might partly account for its effect on reducing the incidence of atrial fibrillation.
Animals ; Benzimidazoles ; pharmacology ; Benzoates ; pharmacology ; In Vitro Techniques ; Kv1.3 Potassium Channel ; drug effects ; Kv1.5 Potassium Channel ; drug effects ; Oocytes ; drug effects ; metabolism ; Patch-Clamp Techniques ; Xenopus
8.Clinical application of anterolateral femoral skin flap pedicled with high site cutaneous branches of the transverse branch of lateral circumflex femoral artery
Pei-Ji WANG ; Qi-Rong DONG ; Jian-Zhong QIN ; Kai-Long ZHOU ; Bo JIANG ; Xian-Zhong ZHANG
Chinese Journal of Microsurgery 2000;0(02):-
Objective To report the clinical effect of free transplanting for soft tissue defects pedieled with high site cutaneous branches of the transverse branch of lateral cirumflex femoral artery.Methods Cu- taneous branches of the descending branch of lateral circumflex femoral artery were found small or abscent in 7 patients.The anterolateral femoral skin flap was pedicled with high site cutaneous branches of the transverse branch to repair the soft tissue defects of the arm,hand,leg and foot,rather than with the descending ones. The size of the flap ranged from 15 cm?6 cm to 28 cm?13 cm,with part muscle valve,iliotibal tract and lat- eral femoral cutaneous nerve.The fractures were performed with internal or external fixation.Results All of the anterolateral femoral skin flap survived well postoperatively in the 7 cases and had good appearance and sensation at one stage.The function of the repaired extremities recovered well.Conclusion The anterolat- eral femoral skin flap pedicled with high site cutaneous branches of the transuvrse branch of lateral circumflex femoral artery has many advantages of good blood supply and large size.The flap was secluding,and can be taken with some muscle and lateral femoral cutaneous nerve.When cutaneous branches of the descending branch of lateral circumflex femoral artery is small or abscent,the anterolateral femoral skin flap with high site cutaneous branches of the transverse branch of lateral circumflex femoral artery is an optimal alternative.
9.The Establishment of Scale-up Isolation Procedure of Phycoerythrin and Phycocyanin from Porphyra yezoensis
Chun-Xia LI ; Shu-Xian WU ; Chun-Er CAI ; Qing WANG ; Si-Hong CHEN ; Hui LI ; Pei-Min HE ;
China Biotechnology 2006;0(01):-
The way of "extracting-salting-chromatography" was used to purify the phycoerythrin and phycocyanin from Porphyra yezoensis in process scale-up.First,by comprehensive comparison of efficiency,the Sephadex G-25 was selected from four resins (Sephadex G-25、G-100、S-300 and CL-6B) as the best choice used in crude extract desalting of phycobiliprotein.Then the preparation process of phycobiliprotein was scaled-up with raw material(Porphyra yezoensis) increased from 1g to 20g,and finally to 400g.The results indicated that the yields of purified phycoerythrin and phycocyanin (absorption spectra purity above 3.2) increased during according to process scale-up,with 0.323% phycoerythrin and 0.148% phycocyanin obtained from 400g frozen Porphyra yezoensis blades respectively.It is no doubt that the process involved in the experiment is a potential way for large scale preparation of phycobiliproteins of high purity.
10.Expression of tissue factor in rabbit pulmonary artery in an acute pulmonary embolism model
Jing-Xia ZHANG ; Yong-Li CHEN ; Yu-Ling ZHOU ; Qian-Yu GUO ; Xian-Pei WANG
World Journal of Emergency Medicine 2014;5(2):144-147
BACKGROUND:Tissue factor (TF) is the initiation factor of the extrinsic coagulation pathway, and plays a critical role in the process of thrombosis. This study aimed to investigate the expression of TF and to explore their clinical effect on the pulmonary artery after acute pulmonary thromboembolism. METHODS:Thirty-four Japanese white rabbits (Level II animals) supplied by Tianjin Medical University were randomly assigned into:group A, specimens of the pulmonary artery taken 3 hours after pulmonary embolism (n=8); group B, specimens of the pulmonary artery taken 8 hours after pulmonary embolism (n=8); group C, specimens of the pulmonary artery taken 24 hours after pulmonary embolism (n=8); and control group, pseudo-operations performed without injection of autologous blood clots (n=10). The animal model of pulmonary thrombo-embolism was established by injection of autologous blood clots into the jugular vein through a 5F catheter, and was confirmed by digital subtraction angiography. The mRNA expression of TF in different parts of the pulmonary artery was accessed by RT-PCR. Theq test was used if there was a significant difference in a given continuous variable among the three groups assessed by ANOVA. The experiment equipment was supplied by the State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, the Chinese Academy of Medical Sciences and Peking Union Medical College. RESULTS:The TF expression in the specimen adjacent to emboli was stable at 3, 8 or 24 hours after embolism. The mRNA expression of TF at 3 and 8 hours after embolism was lower in the specimens taken from the distal end of the morbid pulmonary artery than those adjacent to emboli. While at 24 hours after embolism, there were similar mRNA levels in specimens either adjacent or distal to emboli. CONCLUSION:The high level of TF expression in pulmonary artery tissue adjacent to emboli could lead to locally increased coagulation activity, indicating the necessity of initiating anti-coagulation treatment as soon as possible after acute pulmonary embolism.