As a new method of modern teaching,the computer assisted instruction(CAI) is widely used in every field in modern education. In this paper,the author explores the existed problems and the necessary qualities of using CAI for college teachers in microbiology experimental teaching.

Objective To investigate the trend of blood lipid in female elderly with aging, and further discuss the strate?gies of blood lipid regulation therapy and prevent and control cardiovascular disease during senile period. Methods Physi?cal examination data of 847 elderly female cadres (in 1 837 females) were analyzed to investigate the general level and abnor?mality features of blood lipid in this elderly female group. Results The levels of total cholesterol (TC), triacylglycerol (TG), low density lipoprotein-cholesterol (LDL-C) and body mass index (BMI) were significantly higher in elderly group than those of non-elderly group (P<0.01). The levels of TC, TG, LDL-C, LDL-C/HDL-C, TG/HDL-C, and non-HDL-C increased with aging, and then decreased at the same age group, and reached the peak value at 60 to 69 years old. The level of HDL-C showed a reverse pattern, decreased with aging first and then increased at the same group, and reached the bottom at 60 to 69 years old. Conclusion In this elderly female cadre population, the level of blood lipid changes regularly with aging, indicat?ing the high risk of cardiovascular disease.

Objective To investigate the expression of FSCN1 mRNA in breast carcinoma and benign breast diseases and its relationship to clinicopathological characteristics of breast cancer. Methods Sixty-three cases of breast cancer and thirty cases of benign breast diseases were analyzed by in situ hybridization. Results The expression rates of FSCN1 mRNA in breast cancer tissue and benign breast lesions were 65.1% and 90.0% , respectively (P < 0.05). No relation was found between the expression of FSCN1 mRNA and histopathologic grading, ER, PR, karyokinesis figure, patients′ age, tumor size, lymph node metastasis, TNM stage and c-erbB-2 expression in breast cancer. Survival rates of breast cancer patients with carcinoma expressing FSCN1 mRNA were 92.3% , while those patients with tumors which was negative for FSCN1 mRNA were 82.2%, but there was no significance difference. A significant correlation was observed between FSCN1 mRNA and its protein expression (rs = 0.315, P < 0.05). Conclusion FSCN1 is downregulated in breast carcinoma which may be associated with breast carcinogenesis.

AIM: To observe the humoral immune response in adult rhesus monkey induced by A? 1-15 vaccine. METHODS: 5 adult male rhesus monkeys were injected intramuscularly with A? 1-15 vac cine at baseline and at week 2, 6, 10, 14, 18, 22. The titer and IgG isotypes of the antibody against A? 1-42 in the serum were measured with ELISA. The specificity of the antibody against A? 1-42 was determined by Wester n blotting. The A? plaques in Tg2576 transgenic mouse brain were stained with t he antisera using immunohistochemistry method. RESULTS: At the eighth week after the vaccination, antibody against A? 1-42 bega n to develop significantly i n the serum. The titers of the antibody increased following vaccine boosted and reached 1: 3 840 at the twenty-fourth week, then decreased after the terminat ion o f inocunation. The IgG1 was accounted for the highest level in the antisera pool . The antibody against A? 1-42 showed high specificity. The A? plaques in Tg2576 transgenic mouse brain were labeled with the antisera. CONCLUSION: A? 1-15 vacci ne could induce vigorously specific humoral immune responses in adult rhesus mon key.

Objective To study whether inoculating complete or C terminal fragment of A? 42 vaccine could induce normal rats to produce high titer of antibody against A? 42 or not. Methods The COOH\|terminals(36\|42) of beta\|Amyloid 42 were coupled with different carriers to construct subunit vaccine,which been inoculated in normal SD rats sa did the complete beta\|Amyloid 42.Antibodies against A? 42 of the serum and supernatant of homogenated brain were assessed by Western blottting and indirect ELISA.The morphology of brain,liver,spleen and kidney were observed in paraffin section by HE staining. Results After the second inoculation finished,all experiment groups began to develop antibody against A? 42 ,and the titer raising with inoculating times.After four inoculations,The titers of antibody against A? 42 in every experiment group were over than 1∶10?000.Meantime,low titer of antibody against A? 42 could also be assessed in supernatant of homogenated brain.No morphological damages could be detected in the brain,liver,spleen and kidney of the experiment animals.Conclusion\ Normal SD rats can be induced to develop high titer of antibody against A? 42 after inoculating A? 36\|42 vaccine and A? 1\|42 vaccine.There were no morphological damages in several important organs.\;[

The potamon that were sold by Mengla market were examined positive for two species of paragonimus metacercariae andexcyted metac ercariae, this is the first report in Yunnan Province． The potamon that were sold by cities market from other suburbs may cause epdemic or outbreak of paragonimiasis, there is of momentous significance in epidemiology．

By analyzing data from a questionnaire survey on bilingual teaching, we evalueted the results of bilingual teaching for seven-year medical students and the main problems of bilingual teaching and solutions accordingly. An uneven English level on the part of teachers and students and imperfect materials etc. affect the overall results of bilingual teaching. Therefore persistent efforts need to be made in enhancing the teachers' English level, improving teaching methods and compiling proper textbooks so as to genuinely improve the bilingual teaching program.

Objective To approach rehabilitation nursing measures in using the allogeneic ten-don to re-establish the anterior cruciate ligament of knee through the tibia twin tunnel under arthroscopy.Methods Mental nursing and direct functional exercises was given to 30 patients who received this kind of operation from November,2006 to July,2007,and functional exercises of flection and extention of knee joint by steps,functional exercises of muscle force of quadficeps muscle and back muscle group of legs and the correct using of artificial brace of knee joint.Results Range of joint motion could reach 95°after 2 weeks and 125°after 4 weeks postoperation.Score of joint function(Lysholm)could reach(74.8±5.7)after 6 weeks,(80.0±2.3)after 9 weeks,(91.8±3.4)after 12 weeks, and(94.5±2.2)after 1 year.Conclusion Selective perioperative rehabilitation nursing instruction is the important guarantee of function recovery.

Objective To explore the role of Foxp3+ T cells (Tregs) in liver injury induced by chronic intermittent hypoxia. Methods Thirty-two male Wister rats were divided into four groups:control group (A), high-fat diet group (B), intermittent hypoxia group (C), and high-fat diet and intermittent hypoxia group (D). After 4 weeks, blood samples were collected and livers were surgically removed. Using the standard automatic clinical analyzer to test serum levels of total cholesterol (TC), low density lipoprotein cholesterol (LDL- C), alanina aminotransferase (ALT) and aspartato aminotransferase (AST). The MDA content of liver tissue was measured by colorimetrc method. The levels of TNF-αand IL-1βwere measured by radiommunoassay, and the expression of Foxp3 protein was measured by Western blotting technique. Results Serum levels of TC and LDL-C were significantly higher in B group than those of A, C and D groups, and which were higher in D group than those of A and C groups (P<0.05). There were no significant differences in serum levels of TC and LDL-C between A group and C group. Serum levels of ALT, AST, MDA, TNF-αand IL-1βwere significantly higher in C group than those of A group, and which were significantly higher in D group than those of A, B and C groups (P<0.05). There were no significant differences in these indicators between A group and B group, and between B group and C group. Foxp3 protein expression in liver was significantly lower in D group than that of other groups (P<0.05). Conclusion Foxp3+T regulatory cells involve in the regulation of hepatic injury induced by chronic intermittent hypoxia on the basis of a high-fat diet, and which may play an important role in this process of protective immune response.

Objective Human sperm hyaluronidase in the acrosome is a key enzyme during fertilization.To study and establish a modified substrate film method to improve the diagnotic and treatment of male infertility and investigate the influence of sperm hyaluronidase on male fertility.Methods According to the biochemical feature of sperm hyaluronidase that can dissolve the hyaluronic acid in matrixes,the modified sodium hyaluronate-Gelatin membrane was used as substrate to demonstrate the sperm hyaluronidase activity by incubation and staining.70 human semen samples were selected,categorized and detected for hyaluronidase activity according to the resuh of routine clinical semen examination.The average sperm hyaluronidase activity was statistically anyliezed between the fertile group and infertile group.Results Under general light microscope,the clear and bright digestion circle around the sperm head can be observed against the background of deep purple-blue stained substrate at the positive-reaction area.The amounts of positive reaction and the diameter of the bright circle are positively related to the activity of sperm hyaluronidase.The average hyaluronidase reaction rate and diameter of normal fertile group were 70.84% and 78.17 ?m;What about the infertile group A were 60.02% and 76.92 ?m;The infertile group B were 29.11% and 8.22 ?m; There was statistical difference of HYD activity between the infertile group.B and the fertile group(P