BACKGROUND:Inflammation is an important factor in cervical spondylotic vertebral arteriopathy, bone marrow mesenchymal stem cells have the potential to treat cervical spondylotic vertebral arteriopathy because of the immunomodulatory effects to inhibit inflammation.
OBJECTIVE:To investigate the injury mechanism of vascular injury in the model of cervical spondylotic vertebral arteriopathy and to study the therapeutic effects of bone marrow mesenchymal stem cells on it.
METHODS:Forty Japanese big ear rabbits were randomly divided into four groups:control group, model group, tanshinone group, and stem cellgroup. After modeling, the control and model groups were not given intervention, while the tanshinone and stem cellgroups were injected with tanshinone II A sodium sulfonate solution (10 mL) and bone marrow mesenchymal stem cellsuspension (10 mL) along the ear vein, respectively. After 2 weeks, the routine pathological examination was done to observe the vascular morphological changes, immunofluorescence staining was done to observe the cathepsin B expression in the vertebral artery, and ELISA was used to detect the expression of tumor necrosis factor-αand interleukin-6 in the vertebral artery.
RESULTS AND CONCLUSION:Compared with the model group, the arterial smooth muscle cellhypertrophy and hyperplasia was obviously restrained in stem cellgroup, and vascular endothelial fold was in symmetry, while no significant difference was found between stem cellgroup and tanshinone group. Compared with the model group, expressions of cathepsin B, interleukin 6 and tumor necrosis factor-αexpression were reduced significantly in the stem cellgroup (P<0.05), while there was no difference between the model and tanshinone groups (P>0.05). Inflammatory reaction may be one of mechanisms for vertebral artery damage, and bone marrow mesenchymal stem cells can effectively inhibit inflammation of the vertebral artery and improve vascular remodeling.

Objective_To investigate the potential effect of bone marrow mesenchymal stem cells ( MSCs) on repai-ring the colon epithelial cell,and on the treatment of rats with ulcerative colitis ( UC) .Methods_Monocytes were purified from bone marrow, amplified and identified as MSCs in vitro.Thirty female Wistar rats were randomly di-vided into 3 groups, the normal control, model and MSCs groups (10 rats/group).The rats in model and MSCs groups were induced colitis with trinitro-benzene-sulfonic acid;The rats in normal control group and model groups were injected with 1mL saline via tail vein, while those in MSCs group with 1 mL MSCs suspension.After two weeks, colon tissue samples were analyzed for histopathology,and the colon tissues were made into serial section for determining the distribution of Y chromosome and CK20 double positive cells,analyzing the mRNA levels of CK20,NF-κB, IL-4 by RT-PCR,and assaying colonic NF-κB protein expression with Westen blot,detecting colon tissues IL-4 content with ELISA.Results_Y chromosome and CK20 double positive cells were found in MSCs transplanted colon tissues.The expression of CK20 increased in the colon tissues of UC rats(P<0.01) and in MSCs group in-creased as compared with model group( P<0.01) .The expression of NF-κB increased in the colon tissues of UC rats (P<0.01), but decreased MSCs group as compared with model group (P<0.01).The expression of IL-4 was decreased in the colon tissues of UC rats ( P<0.01) , while in MSCs group it was increased as compared with model group ( P<0.01) .Conclusions_MSCs may have therapeutic efficacy on colitis in rats through differentiating into colon epithelial cells.

Objective: To understand the behavioral characteristics of Chinese college students who have sex with men, and to provide a basis for more targeted health education. Methods: A systematic search of studies related to the high-risk behaviors of MSM of Chinese college students published from January 1, 2015 to November 1, 2018 in the Chinese Journal Full-text database (CNKI), China Biology Medicine database(CBM), PubMed, VIP and Wanfang database was conducted, and relevant data was extracted for Meta-analysis. Results: A total of 12 studies were included, there were 3 839 MSM of college students enrolled in theses study，the results showed that the overall or pooled prevalence of HIV in MSM of college students was 79.1%(95%CI=73.1%-85.0%) in recent years; the combined rate of the number of sexual partners≥2 was 58.9%(95%CI=46.5%-71.3%); the combined rate of condom use for gay sex in the last 6 months was45.3%(95%CI=37.9%-52.7%); the combined rate of condom use for per heterosexual sex was 43.6%(95%CI=29.6%-57.5%); the combined detection rate of HIV was 4.7%(95%CI=2.7%-6.8%). Conclusion The high-risk behavior of MSM among Chinese college students is prominent, and online dating is highly dependent. Therefore, colleges should strengthen the health education of prevention of AIDS.

Objective: To understand HIV/AIDS knowledge, attitude, and awareness of HIV/AIDS policies and regulations among vocational medical students in Sichuan, and to provide scientific basis for AIDS prevention and treatment in higher vocational medical colleges. Methods: A total of 1 458 medical students in a vocational college in Sichuan Province were selected through stratified cluster random sampling and investigated with questionnaire on HIV/AIDS related knowledge, policies and regulations. Results: The average score of AIDS knowledge was(6.63±1.31), and the awareness rate was 82.9%; The average score of attitude towards AIDS was(2.17±0.45), with the highest score being fear and avoidance(2.56±0.61), and the lowest score responsibility perception(1.66±0.58); The total score of HIV/AIDS related policies and regulations was(19.17±2.91). AIDS knowledge was positively correlated with attitude and understanding of policies and regulations(r=-0.24, 0.28, P<0.01) , the higher the knowledge score, the better the attitude and the higher the understanding of policies and regulations. Conclusion HIV/AIDS knowledge of higher vocational medical students has been improved, the fear and avoidance of AIDS is widespread. There are insufficient awareness in AIDS related policies and regulations. Higher vocational medical colleges should be more targeted AIDS health education.

The aims of the present study were to estimate the affinity between 3,5-()-bis(3-methoxy-4-hydroxybenzal)-4-piperidinone hydrochloride (C0818) and heat shock protein 90 (Hsp90) and to investigate the inhibitory effects of this compound on Hsp90 ATPase activity. Fluorescence spectroscopy was used to examine the affinity between varying concentrations of C0818 and Hsp90, N-Hsp90, M-Hsp90 and C-Hsp90. Fluorescence intensities were recorded in the range of 290-510 nm at 293, 303 and 310 K, respectively. A colorimetric assay for inorganic phosphate (based on the formation of a phosphomolybdate complex and the subsequent reaction with malachite green) were used to examine the inhibitory effects of C0818 on Hsp90 ATPase activity. The equilibrium dissociation constantvalue of C0818 was found to be 23.412±0.943 μmol/L. The interaction between C0818 and Hsp90 was driven mainly by electrostatic interactions. C0818 showed the strongest affinity with C-Hsp90. These results conclusively demonstrate the inhibitory activity of C0818 on the activity of Hsp90 ATPase.