Objective:To explore the significance of elevating cervical flap before vital-dye injection in identifying blue-stained nodes receiving lymphatic drainage in rabbit's tongue, so as to provide raw data for normalizing vital-dye injection in sentinel lymph node biopsy (SLNB) during tongue-carcinoma treatment. Methods: 64 adult rabbits were randomly divided into four groups according to the injecting sites: tongue-tip, tongue-lateral, tongue-center and tongue-root. Four doses of methylene blue-0.1, 0.2, 0.3 and 0.4 ml were chosen and injected into 4 tongues in every group, 2 of them were injected before and after the elevation of cervical flap respectively. Time was recorded according to the time points of colored and discolored lymphatic duct and nodes. Results:①The overall success rate in localizing blue-stained node was 93.75%(60/64). ②In the success cases in group elevating cervical flap just before injecting methylene blue, all experiments had the time records of lymphatics and nodes' turning blue; while the counterpart group did not show any blue staining. ③All the blue stained nodes were found in lymph nodeⅠto Ⅲ local area. Conclusion:t may be dependable to utilize methylene blue as tracer in the tongue carcinoma SLNB. Elevation of cervical flap before injecting methylene blue is necessary and practicable in tongue carcinoma SLNB.

This study deeply analyzes the common problems of three military medical universities in the management of undergraduate extracurricular scientific research, such as lack of communication means, limited online resources, backward laboratory opening and low utilization rate of equipment. We have built a cloud platform management system for undergraduate extracurricular scientific research. This system firstly sets up a teaching resources storage module including videos, PPTs, documents, pictures, electronic materials, question bank, etc. Then four subsystems for different roles of students, mentors, experimental teaching staff and administrators are constructed. Finally, this system realizes independent experiments by students, real-time evaluation by mentors, instrument sharing and efficient management through the seamless connection with the user terminal equipment. And the study also makes evaluation on the present usage.

A total of 1 685 school-age children selected from Hangzhou received lung function testing to evaluate the short-term effects of air pollution on their lung function. The results showed that in every 10 μg/m³ increase of average concentration of PM_2.5 and PM₁₀ on the day of the test and the day before the test,peak expiratory flow (PEF) decreased 0.039 (95%CI: 0.012-0.067) L/s and 0.031 (95% CI:0.011-0.051) L/s,respectively. When the average concentration of SO₂ increased 10 μg/m³ on the day of test and the day prior to the test, PEF and 75% of the forced vital capacity that has not been exhaled (MEF₇₅) decreased 0.437 (95%CI: 0.217-0.658) L/s and 0.396 (95%CI: 0.180-0.613) L/s. After being adjusted for NO₂,with every 10 μg/m³ increase of average concentration of PM_2.5 and PM₁₀ on the day of the test and the day before the test,PEF and MEF₇₅ decreased 0.056 (95%CI: 0.028-0.085), 0.053(95%CI: 0.027-0.081) and 0.047 (95%CI: 0.026-0.068) L/s,0.044 (95%CI: 0.023-0.065) L/s on the day before the test, respectively. The results indicate that air pollution have short-term and lag effects on lung function of school-age children in Hangzhou.