Objective:To study the effects of combination of arsenic trioxide and octreotide on the proliferation and apoptosis of hepatocellular carcinoma cells(HCC),and to explore the mechanism of their action on liver cancer.Methods:Heptocellular carcinoma cell SMMC-7721 was treated with arsenic trioxide and octreotide.Cell survival rate was determined by MTT assay;cell cycle and the apoptosis index were determined by flow cytometry (FCM);the morphological changes were observed by transmission electron microscope.Results:The combination of arsenic trioxide and octreotide significantly inhibited the growth of SMMC-7721 and the concentration of each drug in combination is 2.022?mol/L and 8.088?mol/L that was less than single use of arsenic trioxide (3.707?mol/L)and octreotide (22.637?mol/L).The combination of two drugs can arrest HCC in S phases and induce apoptosis.Conclusion:Arsenic trioxide can enhance the antiproliferative effect of octreotide to human HCC and induce apoptosis.

Objective To investigate the chnical effect of levonorgestrel-releasing intrauterine system(LNG-IUS) in patients with adenomyosis.Methods A total of eighty patients with adenomyosis were enrolled and underwent LNG-IUS insertion.The degree of dysmenorrhea,amount of menorrhea,size of the uterus,endometrial thickness,hemoglobin as well as hormonal changes and adverse reaction before treatment and after treatment for 3,6,12 months were compared.Results After treatment for3,6,12months,the amount of menorrhea was reduced [(52.4 ± 8.4)％,(22.6 ± 9.8)％,(14.8 ± 8.6)％ vs.(100.0 ±0.0)％],the scores of dysmenorrhea was reduced [(1.2 ± 0.5),(0.8 ± 0.4),(0.3 ± 0.2) scores vs.(2.4 ±0.6) scores],the level of hemoglobin was increased [(104.4 ± 11.4),(109.2 ± 12.6),(118.8 ± 13.4) g/L vs.(88.6 ± 16.5) g/L],the endometrial thickness was thinner [(6.3 ± 1.4),(3.6 ± 0.8),(1.8 ± 0.5) mm vs.(9.2 ± 2.8) mm],the size of the uterus was reduced [(156.8 ± 56.8),(142.4 ± 48.9),(126.6 ± 36.5)cm3 vs.(172.6 ± 62.5) cm3],there was significant difference compared with that before treatment (P ＜ 0.05).After treatment for 3,6,12 months,the levels of folhcle-stimulating hormone,luteinizing hormone and estradiol had no significant difference compared with those before treatment (P ＞ 0.05).After treatment for 3,6,12 months,the level of cancer antigen 125 was significantly reduced compared with that before treatment [(34.6 ± 11.6),(16.6 ± 9.8),(11.7 ± 6.4) kU/L vs.(62.4 ± 24.3) kU/L].Conclusiom The LNG-IUS has exact therapeutic effect in treatment of adenomyosis,and has little influence on ovarian function.It is worth of using in clinic.

Objective To explore the clinical efficacy of ethinylestradiol and cyproterone acetate (Diane-35) combined with mefformin in patients with polycystic ovary syndrome (PCOS).Methods One hundred and sixty patients with PCOS were divided into control group and observation group by random digits table method with 80 cases each.Patients in the control group were given Diane-35 only,while patients in the observation group were given Diane-35 combined with metformin.The triglycerides (TG),total cholesterol (TC),low density lipoprotein cholesterol (LDL-C),fasting insulin (FINS),fasting blood glucose (FBG),follicle-stimulating hormone (FSH),luteinizing hormone (LH) and testosterone (T) of patients in the two groups were measured before treatment and after treatment 6 months,the rates of pregnancy and abortion were recorded.Results TC,LDL-C,FINS,FBG,FSH,LHt and T of both the 2 groups after treatment decreased obviously than before treatment,and TG in observation group decreased obviously than before treatment,there were statistical differences (P ＜ 0.05).There was no statistical difference in TG in control group between before treatment and after treatment (P ＞ 0.05).After treatment,TG,FINS,FBG,LH in observation group were lower than those in control group [(1.0 ± 0.2) mmol/L vs.(1.6 ± 0.8) mmol/L,(15.2 ± 6.3) mU/L vs.(18.9 ± 6.2) mU/L,(4.5 ± 1.1) mmol/L vs.(5.1 ± 0.9) mmol/L,(6.3 ± 3.0) U/L vs.(7.4 ± 2.9) U/L],there were statistical differences (P ＜ 0.05).After treatment,the rate of pregnancy in observation group was higher than that in control group [61.2％(49/80) vs.42.5％(34/80)],there was statistical difference (x2 =5.633,P＜ 0.05).The rote of abortion in observation group was lower than that in control group [6.1％(3/49) vs.17.6％(6/34)],but there was no statistical difference (x2 =1.694,P ＞ 0.05).Conclusion The clinical effect of Diane-35 combined with metformin in patients with PCOS is better than Diane-35 only.

0. 05). Conclusion :Cordyceps sinensis can inhibit the hepatic fibrosis,and at the same time,doesn't improve the hepatic glycogen synthesis.

Objective To investigate the clinical characteristics and prognosis of young patients with endometrial cancer.Methods Thirty cases with endometrial cancer were selected as our observed group (age less than 40 years old).Forty cases with endometrial cancer were selected as control group and their age were over 40 years old.The information about clinical characteristics,diagnosis,treatment and prognosis of patients in two groups were collected.Results The rates of primary infertility and less than 3 times of pregnancy in observed group were 30.0％ (9/30),56.7％ (17/30) respectively,higher than that in control group (5.0％(2/40) or 30.0％ (12/40)).The differences were statistically significant (x2 =6.183,5.372 respectively,P ＜ 0.05).The positive rate of estrogen receptor(ER) and progesterone receptor(PR) expression were 93.3％ (28/30) and 86.7％ (26/30) in observed group,higher than that in control(42.5％ (17/40),52.5％ (21/40)respectively).The differences were statistically significant (x2 =5.951,4.721 respectively,P ＜ 0.05).Two groups of patients were statistically significant differences in pathological stage,the pathological type of observed group was mainly stage Ⅰ (76.7％ (23/30)),higher than that of control group (47.5 (19/40),and the difference was statistically significant (x2 =5.464,P ＜ 0.05).There was statistically significant difference between the observed and control group on Cervical infiltration and lymph node metastasis(x2 =5.464,1.018respectively,P ＜ 0.05).Conclusion The infeaility and less than 3 times of pregnancy may be the risk factors of younger endometrial cancer and the pathological type of observed group was mainly stage Ⅰ.However the prognosis is similar between the young and the old patients.

The methods for analysis of cosmetics including chemical analysis and instrument analysis, such as spectrophotometry, electrochemical analysis, chromatography (gas chromatography, liquid chromatography and thin-layer chromatography), fluorescence analysis, were summarized in this article in recent years. The prohibited and limited components in cosmetics were determined mainly by chromatography method. The methods for determination of hygienic chemical indexes of cosmetics had been mature. The methods for determination of prohibited and limited components in cosmetics had been studied a little, so should be strengthened more.

Objective:To study the changes of cell cycle phase and cell cycle phase regulation protein during arsenic trioxide induced apoptosis in hepatoma cells,and to explore mechanisms of arsenic trioxide inhibited HCC.Methods:Cultured in vitre,HCC SMMC-7721 were treated 72h with 2?mol/L arsenic trioxide.The cell cycle and apoptosis index were detected by flow cytometry(FCM);the morphological changes were observed by transmission electron microscope.The immunohistochemistry was used to detect the protein expression of p21、cyclin D_1,cyclinA.Results:SMMC-7721 cell cycle was arrested in G_1 and S phase by arsenic trioxide.Compared with control in the course of apoptosis induced by arsenic trioxide p21 protein expressions rose significantly(control:1.128;arsenic trioxide:3.794),while cyclinD_1,cyclinA protein expressions decreased significantly(control 1:3.647,2.833;arsenic trioxide:1.133,1.179).Conclusion:Arsenic trioxide could disturb cell cycle progression of SMMC-7721 and induce apoptosis.The mechanism may relate to that arsenic trioxide up-regulate expression of p21 and down-regulate expression of cyclinD_1,cyclinA.

Objective To evaluate the feasibility of obtaining good quality ultrasound pictures of sutures and anterior fontanelle with three-dimensional and two-dimensional ultrasound. Methods Eighty fetuses at 16-35 weeks of gestation were evaluated with two-dimensional and three-dimensional ultrasound. The sagittal, coronal, lambdoidal and metopic sutures, as well as anterior fontanelle, were inspected. Results The visualization of the sagittal suture of three-dimensional ultrasound was significantly superior to that of two-dimensional ultrasound, while no significant difference in visualization of the metopic, lambdoidal, coronal sutures and anterior fontanelle was found between the two modalities. The visualization of all sutures and anterior fontanelle was significantly superior with three-dimensional ultrasonography to that of two-dimensional ultrasonography before 30 weeks of gestation, whereas no significant difference was found between the two modalities after 30 weeks of gestation. Conclusion Three-dimensional ultrasound can provide more comprehensive information of fetal cranial sutures and anterior fontanelle than two-dimensional ultrasound does. Three-dimensional ultrasound can also supply high visualization of all sutures and anterior fontanelle before 30 weeks of gestation.

Objective To assess the application value of three-dimensional (3D) ultrasound in visualizing fetal cranial sutures and fontanels. Methods One hundred women at different pregnant ages (16-19~+ weeks, 20-24~+ weeks, 25-29~+ weeks, 30-34~+ weeks, respectively) underwent 3D ultrasound, in order to observe the cranial sutures and fontanels of fetuses. Results ①Most cranial sutures and fontanels (70.00%-98.00%) could be visualized with 3D ultrasound. However, the sagittal suture revealed a lower displaying rate (53.00%) than others. ②At different pregnant ages, there was no significant difference of displaying rate of the metopic, coronal, squamoal sutures and the sphenoid, mastoid fontanels between fetuses and there was significant difference of displaying rate of the anterior and post fontanels, sagittal and lambdoid sutures. Conclusion 3D ultrasound can be used as an effective method for visualizing fetal cranial sutures and fontanels.

Object To study the feasibility of flocculation procedure on Bushenhuoxue Decoction, using chitosan instead of alcohol. Methods Using chitosan clarifier to the flocculation procedure of Bushenhuoxue Decoction; selecting the optimum content of chitosan and the density of decoction according to the total extract, and the content of icariin, comparing with the flocculation procedure by alcohol sedimentation. Results Chitosan clarifier could make the decoction clarify as alcohol does. On the retaining of active compounds, the former was better than the later. Conclusion Chitosan can be used to the flocculation procedure of Bushenhuoxue Decoction instead of alcohol.