Purpose:Evaluate the prognostic factors in invasive vulvar cancer.Methods:Fifty one cases with invasive vulvar cancer admitted in our hospital from 1980 to 1999 were retrospectively analyzed.Results:Thirty six patients had squamous cell carcinoma and 27 of them were well differentiated and 6 moderately differentiated and 3 poorly differentiated ,three had adenocarcinoma, three had basal cell carcinoma ,three had leimyosarcoma , six had malignant melanoma. Thirteen cases (25.4%) were in FIGO stage Ⅰ;29 (56.7%) in stage Ⅱ;7(13.1%) in stage Ⅲ and 2(3.9%) in stage Ⅳ. The 5-year survival rates of stage Ⅰ,Ⅱ,ⅢandⅣwere 81.5% , 68.4% , 41.2% and 0.0% (P

Objective:To construct a EGFP(Enhanced green fluorescent protein)-labled euk-aryotic expression plasmid of Fcy::Fur suicide gene and to detect its expression in SKOV3 cell line. Methods:With the technology of gene re-arrangement,Fcy::Fur gene in pORF-Fcy:Fur plasmid was subcloned into pEGFP-N1 vector,with its correctness evaluated by the means of r-estriction enzyme analysis and sequencing.It was transfected into SKOV3 cells with lipofectin,the transient expression of GFP was observed under flu- orescence microscope after 24 hours and detected by Western blot. Results:Correct construction of pEGFP-N1-Fcy::Fur was identi- fied by methods of restriction enzyme analysis and nucleotide sequence determination.A total of 60% transfe-cted cells emitted out green fluorescence under fluorescent microscope after 24 h after transfecti-.on. Fcy::Fur gene expressed by the transfected cells were testified by Western blot. Conclusion:The recombinant eukaryotic expression vectors have been constructed successfully and effec- tive-ly expressed in ovarian cancer cells,which may provide an experimental basis for gene therapy of ovarian cancer.

Objective:To explore the effect of Shenfu Injection (SFI) on expression of Heme oxygenase-1 (HO-1) and iNOS (inducible nitric oxide synthase) in renal failure rats induced by intestinal ischemia-reperfusion (IR) and its possible mechanism. Methods:The model of intestinal ischemia-reperfusion was induced by clamping superior mesenteric artery for one hour and then releasing the arterial clamp for six hours. Wistar rats were randomized into three groups:IR+normal saline group,IR+SFI group and control group (C group). The serum creatinine and blood urea nitrogen were observed respectively. Expression of HO-1 and iNOS in rat kidney tissue was detected by immunohistochemitry and morphometry computer image analysis. The histological change of kidney was observed under light microscope. Results:①Compared with C group,expression of HO-1 and iNOS increased markedly in IR+ normal saline group (P

In the present study, comparison have been made between two kinds of double labelling methods; single side labelling and both sides labelling. To make the different groups be comparable, all sections were continuously cut from the same specimen and all labelling processes were carried out at the same ti- me. The groups were divided as follows: A, one side anti-?-amylase labelling and one side antitrypsin labelling; B, single side labelling of anti-?-amylase and antitrypsin; C, one incubation with anti-?-amylase, followed by two incu- bations with protein A-gold (PAG) complexes of varied size, C1: 7nm and 20nm, C2: 10nm and 20nm, C3: 20nm and 7nm; D, single side labelling of anti- ?-amylase and an unrelated antiserum (antichathepsin D), applying free protein A between two labellings; E, as C, but with free protein A between two PAG incubations; F, as control. Group A and B showed that the two labelling me- thods had almost the same sensitivity.Group C indicated that the interaction of single side labelling were resulted from the combination of the second PAG with the free Fc region of the first antiserum. To decrease the interaction, it was necessary for the second PAG to be much larger than the first one.Group D de- monstrated that the interaction between the second antiserum and the first PAG was very feeble. Group E proved that free protein A could completely prevent the interaction of single side labelling method. The both sides labelling method avoids interaction, but mistakes resulting from the ultrastructural differences on two sides of the sections may happen. Which method to be selected is dependent upon what to be labelled.

Objective:To make clear which drugs can cause drug eruption.Methods:Clinical analysis was made based on the patients data regarding the types of the drup eruption and the severity of liver impairment.Results:There are four types of drugs which can cause drug eruption:antibiotics (55.3%),antipyretic analgesic(26.6%)，Chinese medicine (9.6%) and biological preparations (8.5%).The drug eruptions usually manifested as unticaria,measles-like eruption,multiform erythma and stable eruption.Conclusion:The main allergic drugs are antibiotics and antipyretic analgesic.However,attention should also be payed to the Chinese medicine and biological preparations.

With the deepening of the lung cancer molecular biology research,small molecular targets antitumor drugs make breakthrough progress,the epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) is one of the most attention drug.A series studies show that EGFR-TKI can enhance the antitumor activity of ionizing radiation.Therefore,EGFR-TKI combined with radiotherapy alone for poor-risk patients appears survival benefit,but can't ignore the lung toxicity.However,there is a big contro-versy that EGFR-TKI combined with chemoradiotherapy for locally advanced NSCLC.

AIM: To observe the dysfunciton of myocardial nuclear calcium transport in rat myocardial injury and the effects of liqustraxin on it. METHODS: The model of myocardial damage was induced by subcutaneous injection of isoproterenol (ISO,5 mg?kg -1?d -1). Myocardial nuclei were purified with sucrose density centrifugation and identified zymologically. The activity of Ca 2+-ATPase was measured zymologically and calcium uptake was assayed with 45Ca 2+ isotope. RESULTS: Compared with the control,the Ca 2+-ATPase activity of myocardial nuclear membrane in ischemia group(exprimental) was decreased by 18.1% (P

Objective To investigate the effect of medial collateral ligament reconstruction using semitendinosus autograft.Methods A total of 12 patients with medial collateral ligament injury received knee examinations.Among them,2 had lateral meniscus injury,and received partial resection of the lateral meniscus;2 had cruciate ligament(CL) injury(anterior CL in one and posterior CL in the other),and underwent bone-ligament-bone reconstruction.Medial collateral ligament reconstruction was performed using the semitendinosus tendon autograft ingrowth in the tibial tunnel.Results Primary healing was achieved in all the cases.In 10 of them,the stability of the knee was regained in 6-month follow-up;stress X-ray examination showed that the medial joint space was smaller than the contralateral one by less than 5 mm,and the position of the interference screw was optimal.Of the 12 patients,11 achieved an active knee flexion between 0? and 120?,the other,who had fracture of the lateral condyle of the femur,had a flexion of 90?.Conclusions Semitendinosus autograft provides sufficient tension for medial collateral ligament reconstruction.The fixation of the knee and the closure of the joint capsule can be achieved by using the method.

Objective To study the clinical effect of small incision cataract extraction in the treatment of acute angle-closure glaucoma with cataract.Methods Fifty-eight patients with acute angle-closure glaucoma combined with cataract were selected,and according to the digital table they were randomly divided into two groups:control group and observation group.The patients in the control group were treated by conventional method.The patients in the observation group were treated with small incision cataract.The changes of intraocular pressure,anterior chamber depth and visual acuity before and after treatment were observed.The incidence of complications and the satisfaction of treatment were compared between the two groups.Results There were no significant differences in IOP,visual acuity and anterior chamber depth between the two groups before treatment(all P>0.05).After treatment,the intraocular pressure,anterior chamber depth and visual acuity of the observation group were (10.2±4.0)mmHg,(3.3±0.5)mm,(0.85±0.08),respectively,which of the control group were (17.5±5.0)mmHg,(3.2±0.5)mm and (0.50±0.06),respectively.The improvement of intraocular pressure and anterior chamber depth of the observation group was more significant than the control group(t=0.120,0.520,1.012,all P<0.05).The satisfaction rate of the observation group(96.6%) was higher than that of the control group(75.8%)(χ2=9.823,P<0.05).There was no significant difference in the incidence of complications between the two groups(P>0.05).Conclusion Small incision cataract extraction is an effective treatment for acute angle-closure glaucoma complicated with cataract,and the postoperative complications are less.The patients are satisfied with the treatment and deserve to be popularized.

[Objective] To observe the clinical effect of Jiedu Qinghuo Drink on children infection mononucleosis(IM). [Method] Randomly divide 54 cases into treatment group 27 cases and control one 27 cases. The control one was given Ganciclovir, the treatment one added with Jiedu Qinghuo Drink. [Result] The total effective rate was 85.19% for treatment group, and 55.56% for control one, the comparison of both had difference. [Conclusion] Jiedu Qinghuo Drink has good cure effect on children IM.