Burns ; complications ; Humans ; Multiple Organ Failure ; etiology ; prevention & control

Objective To investigate the expression of microRNA-451 (miR-451) in female invasive ductal carcinoma (IDC) and its roles in tumor cell invasion.Methods Forty five IDC tissues and matched tumor adjacent tissues were collected between January and December in 2014.The expressions of miR-451 in tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR).The relationship between long noncoding RNA taurine regulated genes 1 (lncRNA-TUG1) and clinical features was analyzed by student-t test.miR-451 mimics was transfected into MDA-MB-231 cells.Transwell assay was used to measure cell invasion ability.The expression of c-myc,a potential target of miR-451,and its downstream genes,matrix metalloproteinase (MMP)-2 and MMP-9 were detected by qRT-PCR and Western-blot.Resuts The expression of miR-451 was significantly lower in IDC tissues than in matched tumor adjacent tissues (P ＜ 0.05).Low expression of miR-451 was positively associated with lymphatic metastasis (P ＜ 0.05) and advanced tumor node metastasis (TNM) stage (P ＜ 0.05).Up-regulation of miR-451 in MDA-MB-231 cells could significantly suppress cell invasion ability (P ＜0.05).c-myc expression was down-regulatedwhen miR-451 was transfected (P ＜0.05).As downstream genes of c-myc,the expressions of MMP-2 and MMP-9 were suppressed.Conclusions miR-451 is down-regulated in IDC tissues and associated with cell invasion.miR-451 might inhibit IDC progression by inhibiting c-myc/MMP axis.

OBJECTIVE:To establish a method for identification and determination of illegal adding of chemical substance glibenclamide in traditional Chinese medicine(TCM)for health care,and to provide the reference for the governmental de?partment of drug surveillance and drug inspection.METHODS:TLC and HPLC were employed to isolate and analyze the ex?tract from Qiaoqi capsule suspected of illegal addition of glibenclamide.Glibenclamide was identified by HPLC-DAD spec?trography and ESI-MS technology,and the content determination was made by HPLC.RESULTS:Both capsule sample A and B were detected to contain glibenclamide,the concentrations of which were1.51mg per capsule and0.55mg per capsule respectively.CONCLUSION:The established method is specific,sensitive,and convenient,and can be used efficiently for con?trol and surveillance of illegal adding of chemical substance glibenclamide in TCM for health care.

Objective To explore the clinical effect of IPL combined with tranexamic acid in the treatment of chloasma.Methods 50 patientss with chloasma were selected,and they were randomly divided into two groups by odd-and-even grouping method.The control group received IPL treatment,the study group was given photorejuvenation combined with tranexamic acid treatment.The MASI score,clinical efficacy and adverse reactions were compared between the two groups.Results The MASI score[(8.71 ± 0.82) points] and the total effective rate (92.00％) of the study group were significantly better than those of the control group [(11.35 ± 1.03) points,68.00％,t =3.10,x2 =4.39,all P ＜ 0.05].The incidence rate of adverse reaction betweem the two groups (16.00％,16.00％) had no significant difference (x2 =0.00,P ＞ 0.05).Conclusion Photorejuvenation combined with tranexamic acid has significant clinical effect in the treatment of chloasma.

【Objective】To observe the influence of hot-needle therapy and herbal medicine fuming-washing combined with western medicine for the treatment of rheumatoid arthritis(RA).【Methods】One hundred and twenty-eight RA patients were equally randomized into two groups: group A received hot-needle therapy and herbal medicine fuming-washing combined with western medicine of meloxicam,salazosulfamide,and methotrexate,and group B was treated with western medicine only.The treatment lasted one month.Symptoms and signs of early morning stiffness time,grip strength,joints with tenderness count,tenderness index,auricular rest pain,swollen joints count,swelling index as well as the evaluation by the patients themselves and the doctors were observed before and after treatment.Meanwhile,the changes of rheumatoid factor(RF),C-reactive protein(CRP),erythrocyte sedimentation rate(ESR),the white blood cell(WBC) count and platelet(PLT) count were evaluated before and after treatment.【Results】The total effective rate in group A was 78.1%,which was superior to 53.1% in group B(P

OBJECTIVE: To improve superparamagnetic iron oxide nanoparticles (SPIO-NP) stability, biocompatibility and tumor-targeting and evaluate their tumor targeting in vitro. METHODS: The folic acid-carboxymethylchitosan-superparamagnetic iron oxide nanoparticles (FA-OCMCS-SPIO-NPs) were synthesized by "three-steps": at first, superparamagnetic oxide iron nanoparticles were synthesized by coprecipitation, then, O-carboxymethyl chitosan and folic acid were successfully immobilized on the surface of SPIO-NPs in turn. The fourier transform infrared spectroscopy, X-Ray diffraction were used to confirm their synthesis, meanwhile, transmission electron microscope (TEM), dynamic light scattering (DLS), Zeta-potential measurement and vibrating sample magnetometry (VSM) were applied to characterize their physicochemical properies. Ferrozine assay and Prussian blue staining were used to evaluate their tumor targeting in vitro. RESULTS: X-Ray diffraction showed the crystalline powder of FA-CMCS-SPIO-NPs agree with the standard Fe3O4, Fourier transform infrared results showed O-carboxymethylchitosan and folic acid were covalently modified on the surface of SPIO-NPs successfully, TEM showed all synthesized SPIO-NPs were almost spherical or ellipsoidal. Their sizes were less then 20 nm, dynamic light scattering (DLS), Zeta-potential results demonstrate the intensity particle size distributions of FA-OCMCS-SPIO-NPs were (41.4±0.132)nm, Zeta potential were (-21.36±15)mV. The surface modification may lead to decrese of magnetisms Ferrozine assay and Prussian blue staining results showed FA-OCMCS-SPIO-NPs had good tumor targeting and the tumor targeting had good relations with the amount of FR on surface of tumor cells. CONCLUSION: FA-OCMCS-SPIO-NPs with strong superparamagnetic property, excellent stability, and good folate receptor targeting is successfully synthesized, which demonstrated the potential for tumor MRI diagnose and therapy.

Aim To investigate the effects of Jar-TTA in dual inhibition of glycolysis/oxidative phosphoryla-tion in human esophageal cancer cells and explore the related molecular mechanism. Methods The effect of Jar-TTA on the esophageal cancer cell EC 109 and KYSE-150 viability was examined using MTT assay. The effect of Jar-TTA in apoptosis morphology and mitochondrial membrane potential ( MMP) was observed by a fluorescence microscopy. The apoptosis of cell lines treated with Jar-TTA, the quantitative analysis of MMP falling, as well as the glucose uptake was ana-lyzed by flow cytometry. The mitochondrial OXPHOS and glycolysis of EC 109 cells in response to Jar-TTA were analyzed using a Seahorse XFp extracellular flux analyzer by real-time measurements of the oxygen consumption rate (OCR, indicative of mitochondrial OXPHOS) and extracellular acidification rate(ECAR, indicative of glycolysis). The expression of the proteins related with glycolysis were detected by Western blot. Results Jar-TTA caused strong antiproliferation in EC 109 and KYSE-150 cells in a concentration-dependent manner. 2 , 4 and 8 jimol • L"1 Jar-TTA treat-ments of EC 109 cells for 24 h resulted in a significant increase of early apoptosis population up to (27. 9 ± 6. 1)%, (71.1 ±9.3)% and (65. 0 ±9.5)%, respectively , compared to control treated cells (5. 6 ± 3.2)%. The mitochondrial OXPHOS and glycolysis were significantly inhibited in EC 109 incubated by 4 and 8 p,mol • L"1 Jar-TTA for 2 h. In addition, Jar-TTA induced the drop of MiMP. Furthermore, the glucose uptake and the expression of GLUT4 and LDHA were distinctly inhibited in EC 109 treated by Jar-TTA. Conclusions Jar-TTA induces apoptosis of human e-sophageal cancer cells through dual inhibition of glycolysis and oxidative phosphorylation, which is related with the drop of MMP collapse, the decrease of glucose uptake and the down-regulation of GLUT4 and LDHA in EC 109 treated by Jar-TTA.

Anemia ; blood ; chemically induced ; drug therapy ; etiology ; therapy ; Antineoplastic Agents ; adverse effects ; Blood Transfusion ; Erythropoietin ; blood ; therapeutic use ; Hemoglobins ; metabolism ; Humans ; Neoplasms ; blood ; complications ; drug therapy ; Receptors, Erythropoietin ; blood

Acute Disease ; Case-Control Studies ; Child, Preschool ; Echocardiography, Doppler ; Female ; Heart ; physiopathology ; Humans ; Infant ; Male ; Mucocutaneous Lymph Node Syndrome ; diagnostic imaging ; physiopathology

Objective To investigate the effect of treatment with endostatin combined with siRNA targeting VEGF on rats with collagen-induced arthritis (CIA).Methods Two mg/ml bovinetype Ⅱ collagen was injected into the rat footpad to build up the animal model of CIA.The experimental animal models were treated with endostatin combined with siRNA targeting VEGF 18 days later after immunization and the treatment ended 32 day later.The efficacy was evaluated by the weight,foot and ankle volume of rats.The levels of VEGF in plasma were detected by enzyme-linked immunosorbent assay (ELISA).The VEGF distribution within synovial tissue was detected and examined by immunohistochemical technique.The pathological changes of CIA were evaluated by the pathological changes of the biopsied ankle joints.Student's t test was used to evaluate the experimental data.Results The ELISA test showed that comparing with the model group (17.5±0.3),the endostatin group (15.7±0.3) ng/L and the endostatin combined siRNA targeting VEGF group (14.7±0.5) ng/L showed a significant efficacy in the treatment of CIA in rats (P＜0.05).The endostatin group (135±27) and the endostatin combined siRNA targeting VEGF group (126±71) were different in the number of VEGF in plasma and the VEGF distribution within synovial tissue (P＜0.05),the symptoms of arthritis in these rats were reduced than the model group.Conclusion Endostatin combined siRNA targeting VEGF has good therapeutic effect on rats with CIA.