OBJECTIVE: To investigate the correlation between C-erbB-2 and angiogenesis of breast cancer and its clinical significance.METHODS: The expression of C-erbB-2,VEGF(vascular endothelial growth factor) and MVD(microvessel density) in breast cancer tissues were detected by two-step immunohistochemical method.RESULTS: For the 50 breast cancer specimens,the positive rate was 44 %(22/50) for C-erbB-2 vs.64%(32/50) for VEGF,and the MVD value was 25.96?11.59.The positive expression rates of C-erbB-2 and VEGF and MVD value in patients with lymph node metastasis of breast cancer were all higher than in those without lymph node metastasis of breast cancer(P0.05).C-erbB-2 expression was positively correlated with VEGF expression(P

Objective To sum up the experience of copper needle embolization for huge subcutaneous cavernous hemangioma. Methods Eighteen cases of huge hemangioma, located at the face and neck (3 cases), the upper limbs (5 cases), or the lower limbs and buttocks (10 cases), respectively, were treated from 1996 to 2003. Needles made from copper wire 1~3 mm in diameter were inserted into the lesions, through the skin directly or under the guidance of color Doppler ultrasonography. The copper needles were left in place for 1 week, with or without 4~6 V direct current circulated for 5~10 min twice. Results The therapeutic outcomes included: the shrinking and sclerotization of the hemangioma with the disappearance of postural changes (18 cases), the decrease of circumference of the involved limb (13 cases), the change of the lesion skin from blue-purple to light or normal in color (4 cases), the subsidence or disappearance of swelling and heavy feeling of the involved limb (5 cases), and the regression of the lesion and the reduction of blood flow under color ultrasonography (14 cases). Conclusions Copper needle embolization is a simple, effective and relatively less invasive method for the treatment of huge subcutaneous cavernous hemangioma that is difficult to be resected. It at times may be the only applicable option for children.

Objective:To establish an HPLC method for the determination of methoxsalen and dexamethasone acetate in compound methoxsalen lotions. Methods:An HPLC method was used. The determination was performed on a Sunfire C18 (250 mm × 4. 6 mm,5μm ) column with the mobile phase of acetonitrile-water ( 60∶40 ) at the flow rate of 1. 0 ml·min-1 . The column temperature was kept at 30℃, the detection wavelength was set at 240nm and the injection volume was 20μl. Results:Methoxsalen and dexamethasone acetate showed good linear relationship within the range of 12.71-101.68 mg·L-1(r=0.999 9) and 10.03-80.25 mg·L-1(r=0. 999 7) with the average recovery of 99. 32%(RSD=0. 11%, n=9 ) and 99. 63%(RSD=0. 20%,n=9) , respectively. Conclu-sion:The method is simple, accurate and repeatable, and suitable for the determination of methoxsalen and dexamethasone acetate in compound methoxsalen lotions.

Objective To investigate the correlation between plasma homocysteine level and impaired glucose tolerance(IGT) patients in peripheral neuropathy.Methods 80 patients with IGT were selected according to the results of routine nerve conduction test,including 40 patients associated with peripheral neuropathy (IGT-PN),and 40 patients without peripheral neuropathy (IGT-NPN).Besides,40 healthy subjects were selected as control.Plasma homocysteine levels were measured in the three groups by enzyme rate method.The severity of neuropathy was scored and graded by the Toronto Clinical Scoring System (TCSS).Results Plasma homocysteine levels were significantly higher in the all IGT groups than those in the control group.The plasma homocysteine level in the IGT-PN group (14.2±2.7) μmol/L was significantly higher than that in the IGT-NPN group (12.3±2.6) μmol/L (P＜0.05).Regression analysis showed that plasma homocysteine level had independent effects on IGT with peripheral neuropathy.Plasma homocysteine level was positively correlated with TCSS score.Conclusions Plasma homocysteine may play an important role in the pathogenesis of peripheral neuropathy in patients with IGT,and their level may be associated with the severity of peripheral neuropathy.

0.05).The addition of sIL-6R to group SFT6 restored UCB cells expansion to a higher extent than in group SFT,SFT6 and SFTs(P0.05).Conclusion:The IL-6/sIl-6R in combination with SCF,FL and TPO could enhance the expansion of cord blood CD34+ cells and their expression of CD49d,CD62L and CXCR4.

Objective:To explore the relationship of perfection of middle school students and parental rearing behavior.Methods:392 middle school students were investigated by FM PS and CFMPS.Results:①There was a significant difference in OR between different gender(t=-3.039,P

Objective To investigate the inhibitory effects of traditional Chinese medicine (TCM) on hypermelanosis and provide experimental evidence for treating skin pigmentary disorders. Methods Five TCMs with strong inhibitory effects on tyrosinase activity were tested. The changes of the number and morphology of melanocytes induced by UVB were observed in the experimental hypermelanosis model (brownish guinea pig). Results Decreased melanocytes and melanin granules were found with the treatment of Poria cocos, Polyporus umbellatus, Cornus officinalis Sieb. et Zucc., Atractylodes macrocephala Koidz., Astoagalus complanatus R. Br. ex Bge. Conclusion There are inhibitory effects on hypermelanosis induced by UVB with the treatment of Poria cocs, Polyporus umbellatus, Cornus officinalis Sieb.et Zucc., Atractylodes macrocephala Koidz., Astoagalus complanatus R. Br.ex Bge.

OBJECTIVE:To observe therapeutic efficacy and safety of telbivudine combined with adefovir dipivoxil in long-term treatment of active hepatitis B cirrhosis. METHODS:76 patients with active hepatitis B cirrhosis in our hospital were ran-domly divided into observation group and control group,with 38 cases in each group. Control group was treated with adefovir dip-ivoxil 10 mg,qd;observation group was additionally treated with telbivudine 600 mg,qd,on the basis of control group. Both groups received 2-year consecutive treatment. TBIL,ALB,ALT,AST,rate of HBV-DNA negative conversion and serum virologi-cal index were compared between 2 groups after treatment,and clinical efficacy and ADR were also compared between 2 groups. RESULTS:After treatment,the levels of AST,ALB,ALT and TBIL in observation group were significantly better than the control group,with statistical significance(P<0.05);the rate of HBV-DNA negative conversion,the decreased of serum virus in observa-tion group were significantly higher than control group after 1 and 2 years treatment,with statistical significance(P<0.05). The to-tal effective rate of observation group was significantly higher than that of control group,with statistically significance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Telbivudine combined with adefovir dipivoxil can significantly improve the liver function of patients with hepatitis B cirrhosis and have good long-term therapeutic efficacy with high safety.

Twelve specimens of genital warts were excised from 12 patients and divided into three parts. One part was untreated, the second and the third part were treated with CO_2 laser and microwave respectively. HPV DNA was amplified and detected in 100% of untreated specimens (HPV16 and HPV11 in six patients each), in 83% and 50% of specimens treated with CO_2 laser and microwave respectively. There was a significant difference in the detection rates between untreated and microwave treated specimens (X~2=4.18, P

Objective To investigate the effect of liraglutide and metformin hydrochioride on overweight diabetic patients because of poor glycemic control.Methods Forty-four overweight patients with poor glycemic control were randomly divided to the control group,the liraglutide group and the metformin hydrochioride group.Patients in control group were given diet and exercise control,in liraglutide group and the metformin hydrochioride group were given subcutaneous injection liraglutide,metformin hydrochioride oral treatment respectively for 12 weeks.Body mass index(BMI),fasting blood glucose (FBG),2-hour postprandial blood glucose (2 hPBG) and glycosylated hemoglobin (HbA1c) were measured.Results Compared with pretreatment,FBG,2 hPBG and HbA1c of patients in liraglutide group and metformin hydrochioride group were lower after treatment,and there was significant difference between the two group and the control group after treatment(P ＜ 0.05).BMI of patients in liraglutide group was (24.61 ± 3.47) kg/m2,lower than of the control group((25.37 ± 4.70) kg/m2,P ＜ 0.05).2 hPBG of patients in the liraglutide group was (7.13 ± 3.85)mtmol/L,lower than that of the metformin hydrochioride group ((8.03 ± 4.33) mtmol/L,P ＜ 0.05).FBG level in metformin hydrochioride group ((6.31 ± 3.45) mmol/L) was lower than that of the liraglutide group ((6.98±2.97) mmol/L),but the difference was not statistically significant(P ＞ 0.05).Conclusion Liraglutide and metformin hydrochioride treatment can effectively reduce weight and blood sugar of the overweight diabetics.The effect of liraglutide to reduce postprandial blood glucose and weight of the patient is more significant than of metformin hydrochioride.