Death-associated protein kinase (DAPK) is a muhidomain calcium/calmodulin (CaM)-de- pendent Ser/Thr protein kinase with an important role in apoptosis regulation and tumor suppression. DAPK may serve as a diagnostic and prognostic tool to evaluate disease severity, progression, metastatic rate, and re- currence, and may serve as a target for therapeutic intervention.

Objective To investigate the efficacy of acupoint injection of Astragalus on contents of SOD,HYP and MDA in skin of aging mice in-duced by D-galactose. Methods The mice were randomly divided into normal group,model group,Astragalus group,and saline group(n=15). Except for the normal control group,the mice of other groups were treated with continuous subcutaneous injection of D-galactose to establish mice ag-ing model. The Astragalus group mice was injected with 0.01mL Astragalus in Sanzuli acupoint per day,The saline group mice received an injection of same amount of saline. SOD,HYP and MDA changes in the skin tissue and serum were evaluated after 21 days. Results All the index in the skin tissue and serum showed significant difference(P<0.01)between model group mice and normal control group. Compared with model group,SOD activity and MDA content in the skin tissue and serum of the Astragalus group mice were significantly raised,HYP content was significantly reduced (P<0.01). There also were significant differences of these index between the saline group mice and the model group(P<0.05),but the effect was not as good as the Astragalus group mice. Conclusion Acupoint Injection of Astragalus can prevent and protect against skin aging induced by D-galactose.

Objective There is a significant increase of squamous cell carcinoma antigen ( SCCA) in patients with common skin diseases .The aim of this study was to investigate the difference expression of serum SCCA in patients with common skin diseases . Methods A total of 497 patients with 14 kinds of common skin diseases from the Department of Dermatology were recruited , and the serum SCCA was measured . Results The positive rates of SCCA in 116 patients with psoriasis and 37 patients with erythroderma were 90 .5% and 100%, respectively .The common skin diseases with positive rate of SCCA greater than 60% includes eczema (61.7%), generalized dermatitis (65.1%),pemphigus (61.1%), bullous pemphigoid (62.2%).The SCCA values in pustulosa (1.76 ±1.22 ng/mL) and arthropathica psoriasis (1.69 ±1.05 ng/mL) were decreased compared with pustular (2.86 ±1.46 ng/mL) and arthropathica psoriasis (3.56 ±1.84 ng/mL) (P<0.05). Conclusion The SCCA value may have a relationship with the increase of the performance of keratinization and desquamation in the skin diseases , and it could be used as a valuable indicator for the diagnosis and treatment in patients with cutaneous diseases .

Hemoglobinopathy is a kind of hereditary hematonosis caused by the disproportion among normal hemoglobins or formation of abnormal hemoglobins.Hemoglobin analysis plays an important role in screening and diagnosing hemoglobinopathy.Emerging methods have been applied continuously to hemoglobinometry since the early 20th century.Nowadays,agarose gel electrophoresis,high performance liquid chromatography and capillary electrophoresis are widely used with mass spectrometry as the latest applied method.All these methods have their own features.Appropriate combination of these methods will lead to more scientific and reliable results.

Eight-year medical education is to train medical personnel with high research abi lity and innovative ability, while the experimental teaching, as an important part of physiology teaching, plays an important role in training students in observation, problem-solving skills and practical ability. This article mainly discusses our exploration and practice of teaching in physiology experiment for eight-year program medical students from teaching contents and organization of class teaching, in order to fully increase the studying enthusiasms and meet the scientific mind of these students.

Objective To investigate the role of voltage-gated potassium channel in the human skin squamous cell carcinoma A431 cells and human keratinocyte HaCaT cells. Methods MTT assay was performed to detect the effect of different concentrations of tetraethylammonium (TEA) on the proliferation of cultured A431 cells and HaCaT cells. Besides, enzyme-linked immunosorbent assay (ELISA) was conducted to detect the expression of HERG channel protein in A431 cells, HaCaT cells and normal human skin tissue.Results TEA inhibited the proliferation of A431 cells and HaCaT cells in a dose- and time-dependent manner.After treated with TEA (≥ 10 mmol/L) for 24 or more hours, the proliferation of A431 cells and HaCaT cells was obviously suppressed. A significant difference was observed in the average concentration of HERG channel protein between A431 cells, HaCaT cells and normal human skin tissue (49.7114 ± 3.55696 pg/ml, 35.7471 ±4.14696 pg/ml, 36.8857 ± 3.47810 pg/ml, all P < 0.05). Conclusions The block of voltage-gated potassium channel could inhibit the proliferation of A431 cells and HaCaT cells, and the expression of voltage-gated potassium channel seems to be higher in human skin squamous cell carcinoma cells.

Objective To investigate the effects of photodynamic reaction on cytokines production of HaCat keratinocytes treated with 5-aminolevulinic acid (ALA). Methods The HaCat cells were cocultured in vitro with different concentrations of ALA. Three hours later PpIX levels in HaCat cells were detected, and phtotodynamic reaction was induced by 632.8 nm laser radiation on cells. The cell death was analyzed using a flow cytometer to evaluate reactive intensity. The cytokines contents in supernatant were determined, including interleukin (IL)- lα, IL-6, basic fibroblast growth factor (bFGF), endothelin (ET)-1 and tumor necrosis factor (TNF)-α. Results Different cellular PpIX levels and cell death rates were seen according to ALA concentrations. The cell death rate did not exceed 10% and showed a positive linear relationship with ALA concentration (P＜0.05), when the later was at 0.0-1.6 mmol/L interval. The levels of IL-1α, bFGF and TNF-α increased as ALA concentration ascended at this interval (P＜0.05). No significant correlation was found between ALA concentration and IL-6 or ET-1 level (P＞0.05). Conclusion The photodynamic reactive intensity could be enhanced by increasing ALA concentration. And at certain scope, higher reactive grade might induce higher levels of keratinocyte-derived cytokines.

Objective Diabetic macular edema( DME) is a common complication of diabetic patients.Laser photocoagula-tion therapy is effective in the treatment of DME despite of some patients with unsatisfactory curative effect or recurrence.This study was to explore efficacy and safety of aescin combined with laser photocoagulation in DME treatment. Methods A total of 102 pa-tients with diabetic macular edema in our hospital were randomly divided into control group(51 cases of 67 eyes) and combined treat-ment group (51 cases of 69 eyes).Aescin combined with laser photocoagulation were applied in observation group, and laser photoco-agulation was applied in control group.Observation was made on the changes of intraocular pressure, best corrected visual acuity( BV-CA), central macular thickness(CMT) and the leakage in macular area between the two groups before and after he treatment, along with detection on adverse reactions during the treatment. Results Com-pared with pre-treatment, there were no significant differences be-
tween the two groups in intraocular pressure at 1, 2, 3, 6 months after therapy(P>0.05), and compared with control group in the same time point, the intraocular pressure showed no change significantly in combined treatment group(P>0.05).Compared with con-trol group after therapy, the average value of BVCA in observation group showed significantly decreased at 2, 3, 6 months(Z=2.130, 1.932, 2.283;P=0.037, 0.028, 0.005) while the average value of CMT showed significant increase( Z=1.979, 2.315, 3.08;P=0.045, 0.031, 0.000).Compared with control group at the same time point, the number of patients who suffered from the leakage in macular area significantly decreased in combined treatment group at 2, 3, 6 months(χ2 =4.213, 5.074, 6.625; P=0.040, 0.024, 0.010) .7 patients(13.7%) in combined treatment group suffered from stomach and nausea during the treatment, without any observation of serious adverse reaction. Conclusion Conclusion Aescin combined with laser photocoagulation is effective for the rehabilitation of patients and chconsolidate effect with high safety, which is of great clinical application.

OBJECTIVE:To establish a method for the determination of related substances in Thioctic acid injection. METH-ODS:HPLC was performed on the column of Agilent Eclipse Plus C18 with mobile phase A of 0.005 mol/L potassium dehydroge-nate phosphate solution(adjusted with phosphoric acid to pH 3.0 to 3.1)and B of methanol:aoetonitrile(1∶1,V/V)(gradient elu-tion) at a flow rate of 1.2 ml/min;detection wavelength was 215 nm,column temperature was 35 ℃,and the injection volume was 20 μl. RESULTS:Under the established chromatographic conditions,thioctic acid with impurities and its degradation products were well separated;the linear range of thioctic acid was 0.828-52.992 μg/ml(r=0.999 9);RSDs of precision and stability tests were lower than 3%;recovery of impurity A was 98.45%-105.73%(RSD=2.41%,n=9);the largest single impurity content in mass fraction was 0.152%-0.189%,the total impurity content in mass fraction was 0.487%-0.516%. CONCLUSIONS:The meth-od is simple,accurate,specific and sensitivity,and can be used to determine the related substances of the Thioctic acid injection.

This article was aimed to study enhancing efficacy and reducing toxicity on anti-tumor from total flavonoids and total polysaccharides in Taxus mairei compatibility with taxol. MTT assay was performed to analyze the cell proliferation and apoptosis of 4T1, A549, MCF-7 tumor cells after treatment of total flavonoids and total polysaccharides in T. mairei combined with taxol at different concentrations. Isobolograms was used to investigate the behavior of combination therapy. Therapy experiments were conducted on ICR mice bearing S180 sarcoma. The tumor weight was measured to calculate inhibition rate according to uniform design. Blood cells indicators were detected by blood cell analyzer. The results showed that total polysaccharides and total flavonoids can enhance the inhibition effect on 4T1, A549 cells by taxol. Total polysaccharides can enhance the inhibition effect of taxol to MCF-7 in vitro, and S180 sarcoma-bearing mice in vivo. It reduced the toxic effects of anti-tumor effect of taxol in vivo. It was concluded that taxol can achieve the effect of enhancing efficacy and reducing toxicity through combining with total flavonoids and total polysaccharides in T. mairei.