Objective To study the optimum process for Suanzaoren granules,and to optimize the ethanol-extracting process for jujuboside A in Semen Ziziphi Spinosae.Methods With the extracting rate of jujuboside A as the index,HPLC was used to determine the content of jujuboside A in extracted solution and the extracting conditions were optimized by orthogonal design.Results The optimum extracting condition was adding 8 times amount of 70% ethanol and refluxing for 60 min,then adding 6 times amount of 70% ethanol,refluxing and extracting for 40 min(two times).Conclusion The optimized process is stable and highly efficient.

ObjectiveTo study the missing items in questionnaire cfidentification for TCM constitution.MethodsThe questionnaires were regarded as study objects,which were independently finished by 1322 participants in Changfeng community who were over 45 years and agreed to participate in.The data'was analyzed by SPSS.ResultsMissing items were found in the questionnaires,with a missing rate of 33.74％ on an overall basis.No statistic difference was noted between male and female (x2=0.67,P＞0.05).The rate of missing items increased as participants' age climbing up to the age of 80,and then decreased.Groups characterized with age and gender showed difference in missing items.89.69％ of the incomplete questionnaires had less than 3 missing items,which concentrated in some specific items.ConclusionAttention should be addressed to the fact that missing items were found in questionnaircs of identification for TCM constitution finished independently by residents.

AIM: To examine the changes in immune cells which might be involved in systemic immune dysfunction after severe burns, we detected the sequence of expressed sequence tag in lymphocytes and monocytes of F344 rats post-burn. METHODS: Two pairs of primers were designed depending on the sequence of AI764697.1. SMART RACE technology was employed for amplification of whole sequence. Furthermore, the amplified sequence was testified by Northern blot. RESULTS: A 592bp sequence, which was testified by Northern blot, was suceessfally obtained in our experiments. This product was accepted by GenBank and its accession number is AF244895. CONCLUSION: A cDNA whole sequence in immune cells of F344 rats after severe burns was amplified successfully. More research work should be done to identify the resource, location in genome and function of this product.

Objective: To investigate the expression of angiopoietin-related growth factor (AGF) in obese patients and its relationship with body mass index(BMI), waist circumference(WC), waist-hip ratio(WHR), blood glucose(FBG), plasma insulin(FINS) levels, and HOMA-insulin resistance index(HOMA-IR). Methods: Plasma AGF levels were assayed by ELISA in 40 patients with obesity and 40 normal controls. The relationship of AGF level with BMI, WC, WHR, HbA 1c, blood lipids, FBG, FINS, and HOMA-IR was also analyzed. Results: AGF levels in patients with obesity were significantly increased compared with that in the control group ([177.55±74.09] ng/ml vs [122.37±66.91] ng/ml, P<0.05). We also found that AGF level was positively correlated with Triglyceride(TG), FINS and HOMA-IR(r=0.325, P<0.05; r = 0.451, P<0.01; r = 0.483, P<0.01); and was negatively correlated with high-density lipoprotein cholesterol(HDL, r= -0.529, P<0.01). Multiple regression analysis showed that HDL, BMI and FINS were the factors influencing AGF levels. Binomial Logistic regression analysis indicated that AGF level was positively correlated with obesity after adjusting sex, age, total cholesterol (TC), TG, HDL and low-density lipoprotein cholesterol (LDL) (OR>1). Conclusion: The change of AGF level is associated with the metabolism disorder in obese patients, and it may also contribute to the pathogenesis of obesity.

Objective To explore drinking water pollution by algae in Wuhan. Methods Sampling was carried out in different kinds of tap waters and tank waters for algae detection and identification. Results The detection rate of algae in tap waters was 89% in tank water it was 86% and at the same spot the genera and number of algae in the tank waters increased by 37% and 60% compared with the tap waters respectively. Conclusion Drinking water has been polluted by algae in different degree in Wuhan.

Objective:To compare the efficacy and adverse reactions of omeprazole and pantoprazole in the treatment of patients with peptic ulcer bleeding. Methods: Totally 362 cases of peptic ulcer bleeding were randomly divided into the control group and the observation group with 181 ones in each. The control group was treated by omeprazole 40mg in 0. 9% sodium chloride injection 100ml, ivd, bid, and the observation group was given pantoprazole 80mg in 0. 9% sodium chloride injection 100ml, ivd, bid. The treatment course was 3-6 days. The times of vomiting and melena, bleeding situation and bleeding stopping time in the two groups were observed and recorded, and the clinical efficacy, adverse drug reactions during the treatment and the expense of the two groups were also compared. Results:The bleeding amount on the first and third day in the observation group was significantly lower than that in the control group (P < 0. 05), the average bleeding stopping time and the average times of black stools and vomiting in the observation group were also significantly lower than those in the control group(P < 0. 05). The effective rate of the observation group was 92. 27%, that of the control group was 90. 61%, and the difference was not statistically significant (P >0. 05). The average daily cost of the observation group was significantly lower than that of the control group (P <0. 05). The difference in the incidence of adverse drug reactions of the two groups was not statistically significant (P > 0. 05). Conclusion:The efficacy of omeprazole and pantoprazole in the treatment of peptic ulcer bleeding was similar with high security. However, pantoprazole may reduce the times of vomiting and melena, shorten the bleeding time and decrease the treatment cost.

Objective To evaluate the therapeutic effects of combining acupuncture and physical therapy with drugs on treating Duchenne muscular dystrophy (DMD).Methods Sixty infants with DMD were randomly divided into a treatment group and a control group (n =30 in each).Both groups were injected with Bozhi glycopeptide and given levocamitine and fructose sodium diphosphate orally.Moreover,the treatment group was additionally given acupuncture and physical therapy.Before and after 90 days of treatment,the time to walk 10 metres and to climb stairs of 2 metres high was measured,as were the serum creatine phosphate kinase (CPK),lactate dehydrogenase (LDH) and aspertate aminotransferase (AST) levels of all participants.Results After treatment,the average walking and stair climbing times of both groups were significantly shorter,and those of the treatment group were significantly shorter than those of the control group.Moreover,the average CPK,LDH and AST levels in the treatment group were significantly lower than before treatment and than those of the control group after treatment.There was a significant difference in the overall response rates,with 93％ observed in the treatment group and 73％ in the control group.Conclusion Combining acupuncture and physical therapy with drugs can significantly increase the mobility and improve the serum CPK,LDH and AST levels of children with DMD.

Objective To explore the nutrition support route of enteral nutrition (EN) and parenteral nutrition(PN) on the cellular immune function of patients after esophagectomy.Methods Sixty-two patients with esophageal surgery were randomly divided into EN group and the PN group,and each was 31 cases.Cellular immune indexes(including T lymphocyte subsets,natural killer cells,B lymphocytes) were measured at the preoperation,the postoperative 1st day and 8th day.The t test was preformed to compare the measurement data of two groups,and repeated measures analysis of variance was used to compare multiple time points of measurement data of two groups.Results The levels of CD3,CD4,CD8,CD4/CDS,NK cells and B cells in EN group at before operation were (60.46 ± 6.41) ％,(39.03 ± 5.64) ％,(25.06 ± 5.39) ％,(1.60 ± 0.24),(13.86 ±4.91)％,(13.14 ± 3.81)％ respectively,and those indices at 8th day after operation were (65.18 ±7.81)％,(40.45 ±6.82)％,(22.81 ±4.47)％,(1.78 ±0.16),(16.97 ±4.85)％,(15.69 ± 3.88)％ respectively,and the differences were statistically significant(P ＜ 0.05).The levels of CD4,CD4/CD8,NK cells and B cells in PN group at before operation were (59.78 ± 5.17) ％,(39.10 ± 5.49) ％,(24.94 ± 3.97) ％,1.57 ±0.20,(14.02 ± 4.47) ％,(12.96 ± 3.39) ％ respectively,significantly different from that of 8th days ((59.78 ±5.17)％,(39.71 ±5.63)％,(24.74 ±3.80),(1.62 ±0.15),(15.35 ±4.31)％,(14.33 ±3.43) ％ respectively),and there were significant difference compared to before operation in terms of CD4,CD4/CD8,NK cells and B cells.At the 8th day after operation,there was significant difference between in EN group and PN group in terms of CD3,CD8,CD4/CD8,NK cells and B cells(P ＜0.05).Conclusion Early EN for patients after esophageal carcinoma operation can significantly improve the cellular immune function of patients.

Objective To observe the reactivity of spinal cord transection (SCT) rat abdominal aorta to ?-AR agonists and the infuence of propofol on vascular reactivity, so as to explore the mechanism of autunomic dysreflxia. Methods The rats were divided into sham-operated group and SCT group. 4 weeks after transection of the fourth thoracic spinal cord, the rats were killed, then abdominal aorta rings were adopted to assay their sensitivity to noradrenaline, phenylephrine, clonidine and propofol in isolated organ perfusion system. Results Compared with the rats in sham-operated group, the abdominal aorta reactivity of SCT rats to noradrenaline and clonidine was significantly higher (P

Objective To observe the effect of Tongbi Prescription on pathomorphological changes of ankles in adjuvant arthritis rats. Methods Rats were randomly divided into six groups: normal control group,model control group,three Tongbi Prescription treated group (respectively with high-,mid-and low-dosages),Tripterygium glycosides treated group (each n=10). Except for the rats of normal control group,the others were injected with Freund’s complete adjuvant. The pathomorphological changes of ankles of each group were measured. Results The pathomorphological changes of high-and mid-dosages Tongbi Prescription treated group and Tripterygium glycosides treated group were lightened than those of model control group obviously (P