ObjectiveTo study the missing items in questionnaire cfidentification for TCM constitution.MethodsThe questionnaires were regarded as study objects,which were independently finished by 1322 participants in Changfeng community who were over 45 years and agreed to participate in.The data'was analyzed by SPSS.ResultsMissing items were found in the questionnaires,with a missing rate of 33.74％ on an overall basis.No statistic difference was noted between male and female (x2=0.67,P＞0.05).The rate of missing items increased as participants' age climbing up to the age of 80,and then decreased.Groups characterized with age and gender showed difference in missing items.89.69％ of the incomplete questionnaires had less than 3 missing items,which concentrated in some specific items.ConclusionAttention should be addressed to the fact that missing items were found in questionnaircs of identification for TCM constitution finished independently by residents.

AIM: To examine the changes in immune cells which might be involved in systemic immune dysfunction after severe burns, we detected the sequence of expressed sequence tag in lymphocytes and monocytes of F344 rats post-burn. METHODS: Two pairs of primers were designed depending on the sequence of AI764697.1. SMART RACE technology was employed for amplification of whole sequence. Furthermore, the amplified sequence was testified by Northern blot. RESULTS: A 592bp sequence, which was testified by Northern blot, was suceessfally obtained in our experiments. This product was accepted by GenBank and its accession number is AF244895. CONCLUSION: A cDNA whole sequence in immune cells of F344 rats after severe burns was amplified successfully. More research work should be done to identify the resource, location in genome and function of this product.

Objective To study the optimum process for Suanzaoren granules,and to optimize the ethanol-extracting process for jujuboside A in Semen Ziziphi Spinosae.Methods With the extracting rate of jujuboside A as the index,HPLC was used to determine the content of jujuboside A in extracted solution and the extracting conditions were optimized by orthogonal design.Results The optimum extracting condition was adding 8 times amount of 70% ethanol and refluxing for 60 min,then adding 6 times amount of 70% ethanol,refluxing and extracting for 40 min(two times).Conclusion The optimized process is stable and highly efficient.

Objective To explore drinking water pollution by algae in Wuhan. Methods Sampling was carried out in different kinds of tap waters and tank waters for algae detection and identification. Results The detection rate of algae in tap waters was 89% in tank water it was 86% and at the same spot the genera and number of algae in the tank waters increased by 37% and 60% compared with the tap waters respectively. Conclusion Drinking water has been polluted by algae in different degree in Wuhan.

Objective: To investigate the expression of angiopoietin-related growth factor (AGF) in obese patients and its relationship with body mass index(BMI), waist circumference(WC), waist-hip ratio(WHR), blood glucose(FBG), plasma insulin(FINS) levels, and HOMA-insulin resistance index(HOMA-IR). Methods: Plasma AGF levels were assayed by ELISA in 40 patients with obesity and 40 normal controls. The relationship of AGF level with BMI, WC, WHR, HbA 1c, blood lipids, FBG, FINS, and HOMA-IR was also analyzed. Results: AGF levels in patients with obesity were significantly increased compared with that in the control group ([177.55±74.09] ng/ml vs [122.37±66.91] ng/ml, P<0.05). We also found that AGF level was positively correlated with Triglyceride(TG), FINS and HOMA-IR(r=0.325, P<0.05; r = 0.451, P<0.01; r = 0.483, P<0.01); and was negatively correlated with high-density lipoprotein cholesterol(HDL, r= -0.529, P<0.01). Multiple regression analysis showed that HDL, BMI and FINS were the factors influencing AGF levels. Binomial Logistic regression analysis indicated that AGF level was positively correlated with obesity after adjusting sex, age, total cholesterol (TC), TG, HDL and low-density lipoprotein cholesterol (LDL) (OR>1). Conclusion: The change of AGF level is associated with the metabolism disorder in obese patients, and it may also contribute to the pathogenesis of obesity.

ObjectiveTo determine the primary control factors of nucleotides metabolisms on insulin secretion.MethodsNMRI mice isolated islets were used to investigate the amount of insulin secretion and the changes of ATP,ADP,GTP,GDP and UTP in islets when the activity of K+ATP channel was eliminated by diazoxide.ResultsThe above assays demonstrated that insulin secretion increased with the rise of glucose concentration.In the same time,ratio of ATP/ADP,GTP/GDP and level of UTP increased,too.All three indexes correlated with the insulin secretion very well respectively,but only ratio of ATP/ADP can control insulin secretion directly in common influence.ConclusionOur findings suggest that there is the second control site in islets when glucose induces insulin secretion and ATP/ADP ratio is only key factor in this mechanism.

Objective:To compare the efficacy and adverse reactions of omeprazole and pantoprazole in the treatment of patients with peptic ulcer bleeding. Methods: Totally 362 cases of peptic ulcer bleeding were randomly divided into the control group and the observation group with 181 ones in each. The control group was treated by omeprazole 40mg in 0. 9% sodium chloride injection 100ml, ivd, bid, and the observation group was given pantoprazole 80mg in 0. 9% sodium chloride injection 100ml, ivd, bid. The treatment course was 3-6 days. The times of vomiting and melena, bleeding situation and bleeding stopping time in the two groups were observed and recorded, and the clinical efficacy, adverse drug reactions during the treatment and the expense of the two groups were also compared. Results:The bleeding amount on the first and third day in the observation group was significantly lower than that in the control group (P < 0. 05), the average bleeding stopping time and the average times of black stools and vomiting in the observation group were also significantly lower than those in the control group(P < 0. 05). The effective rate of the observation group was 92. 27%, that of the control group was 90. 61%, and the difference was not statistically significant (P >0. 05). The average daily cost of the observation group was significantly lower than that of the control group (P <0. 05). The difference in the incidence of adverse drug reactions of the two groups was not statistically significant (P > 0. 05). Conclusion:The efficacy of omeprazole and pantoprazole in the treatment of peptic ulcer bleeding was similar with high security. However, pantoprazole may reduce the times of vomiting and melena, shorten the bleeding time and decrease the treatment cost.

Objective To evaluate the therapeutic effects of combining acupuncture and physical therapy with drugs on treating Duchenne muscular dystrophy (DMD).Methods Sixty infants with DMD were randomly divided into a treatment group and a control group (n =30 in each).Both groups were injected with Bozhi glycopeptide and given levocamitine and fructose sodium diphosphate orally.Moreover,the treatment group was additionally given acupuncture and physical therapy.Before and after 90 days of treatment,the time to walk 10 metres and to climb stairs of 2 metres high was measured,as were the serum creatine phosphate kinase (CPK),lactate dehydrogenase (LDH) and aspertate aminotransferase (AST) levels of all participants.Results After treatment,the average walking and stair climbing times of both groups were significantly shorter,and those of the treatment group were significantly shorter than those of the control group.Moreover,the average CPK,LDH and AST levels in the treatment group were significantly lower than before treatment and than those of the control group after treatment.There was a significant difference in the overall response rates,with 93％ observed in the treatment group and 73％ in the control group.Conclusion Combining acupuncture and physical therapy with drugs can significantly increase the mobility and improve the serum CPK,LDH and AST levels of children with DMD.

Effects of salviae miltiorrhizae (SM) and SOD on serum and tissue (liver, intestine) lipid peroxidation were studied on rabbits in reperfusion after hemor rhage shock.The results showed that both SM and SOD could effectively reduce the serum MDA,while the inhibitory effect of SM on tissue (intestine) lipid Peroxidation was more significant than that of SOD.90 min after shock,18 rabbits were equally divided into 3 groups,one third of shed blood was re-infused and simultaneously teperfused with balanced salt solution (BSS) (group A), SOD in BSS (group B) and SM in BSS (group C). After reperfusion, serum MDA increased significantly in group A, while serum in MDA in group B and C decreased gradually and was significantly lower than that of group A after reperfusion of 3 hr (I3). Intestine MDA in group C was evidently lower than that in group A (P

AIM:The limitation of materials' strength results in the external fixation of patients with patellar fracture following absorbable screw fixation.This study was aimed to guide the patients do progressive rehabilitation and observe the recovery of knee joint function.METHODS:Totally 35 patients with patellar fractures were enrolled from the Sixth People's Hospital in Shanghai Jiao Tong University between June 2004 and June 2006,including 27 cases of transverse fractures and 8 cases of comminuted fractures.They were all treated with BIOFIX self-reinforced poly-lactide absorbable screws,which were produced by Finland BIONX Company.Postoperatively isometric contraction training of quadriceps femoris was followed,and then progressive knee joint range of motion was carried out.The curative effect was evaluated by the recovery of knee joint function and radiograph.Normal knee joint function was taken as excellent effect,while nearly normal knee joint function and slightly poor squatting were taken as good effect.RESULTS:All the patients completed the treatment and rehabilitation,followed up for no less than 12 months,with a mean of 22 months.The average healing time was 6 weeks,excellent result cases were seen in 29 patients and good in 6.There was two cases occurred postoperative complications(wound swelling).CONCLUSION:Self-reinforced poly-lactide absorbable screws for treatment of patellar fractures and appropriate postoperative rehabilitation is believed to prevent the second operations.