BACKGROUND:Currently, a large number of studies have confirmed that macrophage migration inhibitory factor plays an important role in a variety of biological activities, such as tumor development. In recent years, it also plays an important role in the inflammatory process, and has achieved a lot of results. However, it is unclear whether and how the macrophage migration inhibitory factor plays a role around the oral implant under oral environment.
OBJECTIVE:To investigate the effect of macrophage migration inhibitory factor on the inflammation of bone marrow mesenchymal stem cels growing around the titanium implant.
METHODS:First, bone marrow mesenchymal stem cels were seeded onto titanium cel culture disks to simulate the peri-implant environment in the mouth, and then, the cels were divided into four groups: control group, without any stimulation; lipopolysaccharide group, lipopolysaccharide-induced inflammation of bone marrow mesenchymal stem cels; non-specific smal interfering RNA (siRNA)+lipopolysaccharide group, non-specific siRNA-transfected and lipopolysaccharide-induced cels; macrophage migration inhibitory factor siRNA+lipopolysaccharide group, cels under the stimulation of lipopolysaccharide were transfected with macrophage migration inhibitory factor.
RESULTS AND CONCLUSION: Using flow cytometry, the cels expressing over 95% CD29 and CD90 as wel as less than 5% CD 45 were selected in the experiment. Cel counting kit-8 test showed that macrophage migration inhibitory factor siRNA+lipopolysaccharide had no influence on the proliferation of bone marrow mesenchymal stem cels. Lipopolysaccharide significantly stimulated the inflammatory reactions of bone marrow mesenchymal stem cels, which was 15-20 times of the control group (P < 0.01). However, compared with the lipopolysaccharide group, the levels of interleukin-1β, interluekin-6 and tumor necrosis factor-α were increased significantly after transfection with macrophage migration inhibitory factor siRNA+lipopolysaccharide stimulation (P < 0.01). These findings indicate that lipopolysaccharide can promote inflammation of bone marrow mesenchymal stem cels around the oral implant, but macrophage migration inhibitory factor siRNA can, to some extent, inhibit the occurrence of inflammation.

Objective To evaluate the clinical application of transrecral ultrasound in the treatment of prostate cancer.Methods 23 cases of prostate cancer treated with PSA,CT,MRI and postoperative pathological confirmation were analysed respectively and the results of transrectal ultrasound were compared.Results The diag- nostic match rate of transrectal ultrasound for prostate cancer was 77%.Transrectal ultrasound showed hypoecho nodes in prostate.Conclusion Transrecral ultrasound could detect prostate cancer.

MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression at the post-transcriptional lev-el and then affect important physiological and pathological processes. Many microRNAs are tissue or organ-speciifc which make it possible for them to become potential biomarkers. Similarly, microRNAs are important for physiological functions of kidney and they are involved in various renal disorders. The review summarizes current information about microRNA effect on various kidney diseases, in order to provide references for the diagnosis and treatment of kidney disease in the future.

Lipoxins are metabolic products of arachidonic acid,which possess a wide spectrum of adjustment function for various inflammatory cell function and the expression of inflammatory related genes.It is known asstop signals or braking signals of inflammatory response,which can promote the regression of inflammation through regulating a variety of inflammatory signaling pathway.Now,the progress in the regulation of multiple signal pathways by lipoxins and its anti-inflammatory mechanism are reviewed.

BACKGROUND:With the development of sports medicine and rehabilitation medicine, electromechanical delay has been looked as an important index for evaluating the neuromuscular function at abroad. But the relevant research is little reported in China. OBJECTIVE:To review the literatures related to electromechanical delay published in recent years, and to explore the mechanisms, influential factors and the application status of the electromechanical delay, thereby providing reference for clinical practice and research. METHODS:A computer-based search of CNKI, WanFang and PubMed databases was performed for articles addressing electromechanical delay published from February 1979 to February 2017. The keywords were electromechanical delay, electro-mechanical response time in English and Chinese, respectively.Repeated and old studies were excluded, and finally 44 eligible literatures were included, including 3 Chinese and 41 English articles. RESULTS AND CONCLUSION: The mechanisms of electromechanical delay have been clarified. The type of muscle fiber and the level of muscle fatigue can influence electromechanical delay, but the underlying mechanisms still remain unclear. Whether age and gender make effect on electromechanical delay is controversial. Electromechanical delay is not only used for evaluating the athletes' ability to reaction, but also wildly used to investigate the mechanism of various sports injuries and evaluate the effectiveness of rehabilitation.

Acute kidney injury is a clinical common disease,but it is a dangerous illness with higher o-verall mortality.Even mild renal insufficiency carries complications.Therefore,it is very important for the early diagnosis of acute kidney injury,which is based on high sensitivity,strong specificity of biomarkers.

Animals ; Cells, Cultured ; Interleukin-1 ; pharmacology ; Matrix Metalloproteinase 1 ; secretion ; Muscle, Smooth, Vascular ; cytology ; Myocytes, Smooth Muscle ; cytology ; drug effects ; metabolism ; Platelet-Derived Growth Factor ; pharmacology ; Rabbits

Objective To investigate the clinical significance of three-dim en sional CT in diagnosis and treatment of posterior pelvic ring fractures. Methods A retrospective analysis was done for 19 cases of posterior pelvic ring fractur es who were treated from March 2002 to August 2003 and had plain and three-dime nsional CT films. Their radiological characteristics and results were compared b etween the 2 kinds of film. Results 3-D CT corrected the misdiagnosis or uncert ainty of 9 cases who had plain radio-graphs. The 3-D CT showed obvious advanta ges over plain radio-graphs in detecting sacroiliac diastasis, sacroiliac joint fragments, fractures involving iliac and sacral lips abutting the sacroiliac jo int, sacral fracture and complicated comminuted fracture, because it clearly del ineated fractures of posterior pelvic ring and manifested the severity of commin ution and separation. Conclusion The 3-D CT scan provides valuable information for precise diagnosis and treatment plan for the complex posterior pelvic ring f ractures.

AIM: To explore the effect and the mechanism of sulphated heparin on the proliferation and the apoptosis of human hepatocellular carcinoma cells. METHODS: The human hepatocellular carcinoma cell line (HepG-2) was used to identify the expression of ras gene protein and to study the effect of sulphated heparin on proliferation and the apoptosis in vitro . RESULTS: The sulphated heparin downregulated the ras protein expression and inhibited the cell growth in HepG2 cells. In the presence of sulphated heparin, the apoptosis rate of HepG2 increased. CONCLUSION:The data suggest that the effects of sulphated heparin on the proliferation and the apoptosis of the human hepatocellular carcinoma cell are correlated with the signaling transduction mediated by ras gene protein.

After the problems in digitalizing ancient books on traditional Chinese medicine were analyzed, the principles that should be followed in their digitalization were pointed out according to its theories and practice.The platform for digitalizing them was constructed with《Collective Notes to Canon of Materia Medica》as an example, and its design ideas, framework, development model and functional modules were described.