Objective To investigate the effects of self-made Yishen Antai Decoction combined with progesterone in the treatment of recurrent spontaneous abortion (RSA) and the effect on pregnancy outcomes and blocking antibodies (BA). Methods 100 cases of patients with RSA were randomly divided into the observation group and the control group, 50 cases in each group. The control group was treated with progesterone and on the basis, the observation group was additionally treated with self-made Yishen Antai Decoction. Both groups were intervened till 12 weeks of gestation, and the clinical curative effect was evaluated. Results The total effective rate of treatment, the success rate of pregnancy and the positive rate of blocking antibody (BA) showed significant differences between the observation group and the control group (P< 0.05). After treatment, the total TCM symptom score and serum levels of interleukin-2 (IL-2) and interferon-γ (IFN-γ) were significantly lower while interleukin-10 (IL-10) level was significantly higher in the observation group than the control group (P< 0.05). There were no serious adverse reactions in the two groups. Conclusion Self-made Yishen Antai Decoction is effective and safe in the treatment of RSA. It can significantly improve the success rate of pregnancy and the positive rate of BA, which may be associated with the regulation of IL-2, IL-10 and IFN-γ levels.

Objective Anovel index based on frequency-domain analysis of heart rate variability (HRV) was tested on patients with reduced left ventricular systolic function.This index,namely VHFI,was defined as the very high frequency (VHF) component of the power spectrum normalized to represent its relative value in proportion to the total power minus the very low frequency component.Methods Patients (n=130) were divided into a study group,consisting 66 patients with decreased left ventricular systolic function,and a control group,consisting 64 patients with normal heart structure and function and without severe coronary artery stenosis (＜50%).Results VHFI in the study group was significantly higher than that in the control group (19.17±13.35 vs 11.37±10.77,P＜0.001).Cardiac events occurred in 18 patients during follow-up (33.34±3.26 months).Defining the positive test as VHFI =15 and negative test as VHFI ＜15,achieved a sensitivity of 57.58% and a specificity of 78.13% for predicting decreased left ventricular systolic function,and achieved a sensitivity of 66.67% and a specificity of 64.29% for predicting cardiac events.Univariate Cox regression analysis showed that positive VHFI test was an independent variable in predictive cardiac events.Conclusions The results suggest that VHFI is a useful tool for quick evaluation of left ventricular systolic function and prediction of prognosis.

Objective To make an evidence-based rehabilitation plan for a patient with osteogenesis imperfecta after external fixation. Methods Based on a comprehensive assessment for the patient's condition, the clinical problems were listed. Related articles were searched from National Guideline Clearinghouse (NGC), The Cochrane Library, PubMed, CBM, CNKI and Wanfang databases. And the reference list of each eligible article was also included. Finally the optimum rehabilitation program was designed with optimal clinical evidence, patient's conditions and relatives' willingness. Results 1 randomized controlled trial and 4 case reports were included. A progressive exercise program was developed and implemented. No new fractures occurred during the intervention. Muscle strength, gait, activities of daily living had been greatly improved. 20 weeks later, the Berg score was 43 and the Barthel index was 80. Conclusion The progressive rehabilitation exercise program developed from evidence-based practice is safe, effective and feasible.

Objective To investigate the expression of microRNA-451 (miR-451) in female invasive ductal carcinoma (IDC) and its roles in tumor cell invasion.Methods Forty five IDC tissues and matched tumor adjacent tissues were collected between January and December in 2014.The expressions of miR-451 in tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR).The relationship between long noncoding RNA taurine regulated genes 1 (lncRNA-TUG1) and clinical features was analyzed by student-t test.miR-451 mimics was transfected into MDA-MB-231 cells.Transwell assay was used to measure cell invasion ability.The expression of c-myc,a potential target of miR-451,and its downstream genes,matrix metalloproteinase (MMP)-2 and MMP-9 were detected by qRT-PCR and Western-blot.Resuts The expression of miR-451 was significantly lower in IDC tissues than in matched tumor adjacent tissues (P ＜ 0.05).Low expression of miR-451 was positively associated with lymphatic metastasis (P ＜ 0.05) and advanced tumor node metastasis (TNM) stage (P ＜ 0.05).Up-regulation of miR-451 in MDA-MB-231 cells could significantly suppress cell invasion ability (P ＜0.05).c-myc expression was down-regulatedwhen miR-451 was transfected (P ＜0.05).As downstream genes of c-myc,the expressions of MMP-2 and MMP-9 were suppressed.Conclusions miR-451 is down-regulated in IDC tissues and associated with cell invasion.miR-451 might inhibit IDC progression by inhibiting c-myc/MMP axis.

Objective To study the process of the root development of Codonopsis pilosula as well as theaccumulatinglocationsofthemainmedicinalcompositionandstoragenutritionintheroot. MethodsThe development process of the roots was studied by paraffin section methods. Storage nutrition was loca-lized by PAS reaction and Sudan Ⅲ reaction. The medicinal composition of C. pilosula, terpene and steroid were stained with mix reagent of 5% vanillin-acetic acid and perchloric acid. Results The development process of the root is shown as four stages: promeristem, primary meristem, primary structure, and secondary growth. Abundant laticifers exist in mature root and the prime laticifers could be found in phloem as early as the first vessel of primary xylem appears. Then, the laticifers exist in phloem at all time. Starch granule accumulated in mature root as reserved substance. Terpene and steroid accumulated in parenchyma and laticifer. Conclusion Parenchyma and laticifer in the roots of C. pilosula are the main parts of the roots to store medicinal composition and storage nutrition.

Objective To present a solution of the hospital information management access control for more secure information sharing. Methods Several commonly used access control models were compared. Some problems of the RBAC model were analyzed in the hospital information circumstances. Results A solution of space-based data access control was presented on the base of the role-based access control (RBAC) module. Conclusion The space-based data access control method can reduce space consumption and maintain data consistency, which is more suitable for the use of the hospital management information system.

Objective:To explore therapeutic effect of pharyngeal treatment machine (PTM) combined resuscitating acupuncture (RA) on patients with dysphagia after stroke.Methods: A total of 98 stroke patients with dysphagia were selected.According to random number table, they were randomly and equally divided into pure pharyngeal treatment group (received pure PTM therapy based on routine treatment) and combined treatment group (received RA based on pure pharyngeal treatment group).Video fluorography (VFG) score and swallow-quality of life (SWAL-QOL) score before and after treatment, and total effective rate were evaluated and compared between two groups.Results: Compared with before treatment, there was significant rise in VFG score, and significant reduction in SWAL-QOL score in both groups after treatment, P<0.01 all;compared with pure pharyngeal treatment group after treatment, there was significant rise in VFG score [(5.46±2.04) scores vs.(6.54±2.75) scores], and significant reduction in SWAL-QOL score [(741.33±25.92) scores vs.(632.54±26.77) scores] in combined treatment group, P=0.032, 0.001 respectively.Total effective rate of combined treatment group was significantly higher than that of pure pharyngeal treatment group (93.88% vs.75.51%), P=0.014.Conclusion: Compared with pure pharyngeal treatment machine, pharyngeal treatment machine combined resuscitating acupuncture possesses better therapeutic effect for recovery of swallowing function, which is worth extending.

Objective To evaluate the clinical significance of laparoscopic operations in the treatment of ovarian endometriosis cysts. Methods We compared clinical data between 52 cases of laparoscopic operation for ovarian endometriosis cysts (Laparoscopy Group) and 75 cases of open operation for ovarian endometriosis cysts (Open Group) in the same period. Results The operation time was significantly shorter in the Laparoscopy Group (61 4?9 3) than in the Open Group (72 6?7 1) ( t =7 692, P =0 000). The time to first flatus was significantly earlier in the Laparoscopy Group (25 3?4 1 h) than in the Open Group (49 8?6 9 h) ( t =22 935, P =0 000). The postoperative pyrexia rate in the Laparoscopy Group (9/52) was significantly lower than that in the Open Group (31/75) ( ? 2 =8 216, P =0 004). There were no significant differences on the remission rate of dysmenorrhea between the Laparoscopy Group (26/37) and the Open Group (34/51) ( ? 2=0 128,P =0 818) and on the postoperative pregnancy rate between the Laparoscopy Group (5/22) and the Open Group (7/25) ( ? 2 =0 171, P =0 747). Conclusions Laparoscopic operation for ovarian endometriosis cysts is as effective as open operation, being an ideal alternative.

OBJECTIVE To provide evidences for working out methods of nosocomial infection surveillance on the basis of investigation of nosocomial infection in our hospital.METHODS By efforts of the professional staff of hospital,with cooperation of the clinic personnel infection management group,all patients were investigated by bed-visiting and patients medical records checking.The data were analyzed.RESULTS Of the 1261 patients investigated,the prevalence rate of NI was 5.15%.The common sites of nosocomial infections were lower respiratory tract(32.31%),upper respiratory tract(16.92%),surgical wound(9.23%)and peritoneal cavity(6.15%).CONCLUSIONS The prevalence survey is a rapid and efficient method,which could offer evidence of target surveillance of NI.

Objective To evaluate the value of laparoscopy in the treatment of intramural myoma.Methods Totally 120 cases of intramural myoma were randomly divided into laparoscopy(n=52)and open groups(n=68).Clinical data,including operation time,intraoperative blood loss,rates of postoperative pyrexia and postoperative complications,length of hospital stay and follow-up,were compared between the two groups.Results Compared to the open group,the laparoscopy group had significantly longer operation time (106.3?54.9)min vs(66.5?7.3)min,t=5.918,P=0.000],less blood loss 10-300 ml(median,50 ml)vs 20-200 ml(median,50 ml),Z=-1.998,P=0.046],lower rate of postoperative pyrexia 7.7%(4/52)vs 30.9%(21/68),?2=9.608,P=0.002],similar rate of postoperative complications 3.8%(2/52)vs 8.3%(3/36),?2=0.000,P=1.000],shorter hospital stay (7.5?1.4)d vs(10.4?1.2)d,t=-12.201,P=0.000],and similar length of follow-up (9.7?2.3)months vs(9.6?1.8)months,t=0.267,P=0.790] and rate of normal menses 94.4%(17/18)vs 90.5%(19/21),?2=0.000,P=1.000].Conclusions Laparoscopy is as effective as open surgery for intramural myoma.While the laparoscopy leads to better postoperative recovery than the open surgery.