Objective To explore the clinical effects of sitagliptin alone or its combination with metformin on the patients with type 2 diabetes registered on their first clinic/hospital visits.Methods Eightytwo patients with type 2 diabetes who diagnosed with oral glucose tolerance test (OGTT),insulin test and glucagons stimulating C-peptide test from May 2011 to February 2012 in our hospital were randomly divided into two groups:the patients in control group took sitagliptin alone (control group,n =41) and the patients in experimental group took sitagliptin combined with metformin (experimental group,n =41).Eighty-two patients,after the mission,were given diabetic diet and moderate exercise therapy.Taking sitagliptin 100 mg,1 times/d;combined with metformin groups given sitagliptin 100 mg/d,metformin 250 mg,3 times/d.Six months later,the major indexes comparing between before and after treatment of weight,body Mass Index (BMI),glycosylated hemoglobin(HbA1c),OGTT,fasting plasma glucose (FPG),2 h plasma glucose (2 hPG),fasting insulin (FINS),2 h postprandial insulin (2 hINS),fasting C-peptide and 2 h postprandial C-peptide levels were measured to research the changes during the treatments.Results After treatments,all the indexes were decreased significantly,and the BMI,HbA1c,FPG,2 hPG,FINS,2 hINS,fasting C-peptide and 2 h postprandial C-peptide in the group with sitagliptin united with metformin were significantly lower than the group with sitagliptin((27.4 ± 1.2) kg/m2 vs.(27.9 ±1.5) kg/m2,t =-2.812;(8.1 ±1.2)％ vs.(8.8 ± 1.3)％,t =-2.953;(8.17 ±1.22) mmol/L vs.(9.22 ± 1.28) mmol/L,t =-4.940;(10.16 ± 1.68) mmol/L vs.(11.01 ± 1.62) mmol/L,t =-3.362; (9.22 ± 1.93) mU/L vs.(9.85 ± 1.59) mU/L,t =-2.212;(74.62±13.73) mU/L vs.(83.75 ± 13.12) mU/L,t =-5.235;(1.43 ±0.34) μg/L vs.(1.77 ±0.27)μg/L,t =-3.194; (6.80 ± 1.40) μg/L vs.(7.61 ± 1.64) μg/L,t =-4.480 ; P ＜ 0.05).Conclusion Sitagliptin is useful to treat type Ⅱ diabetes,plays important roles in improving patients' quality of life and insulin resistance,control of blood glucose,reduce the family and the burden of social health,and the effect will be more better when united with metformin.

Objective To investigate the changes of coagulation and fibrinolysis systems in patients with solid cancer. Determine the mechanism of thrombosis formation in solid cancer and the the metastasis mechanism of solid cancer.Methods To measure plasma concentrations of TF,TFPI,t-PA,u-PA,PAI-1 by ELISA and test protein C activity(PC:A)by chromogenic substrate assay.Results Plasma concentrations of TF,TFPI,u-PA,PAI-1 were all higher in solid cancer patients than normal control and higher in metasta- sized team than non-metastasized team.In the dead team,u-PA and PAI-1 were higher while TFPI was low- er. t-PA was higher in cancer patients combined with venous thromboembolism while protein C activity was lower.Conclusion Disorders of coagulation and fibrinolysis system are related to the thrombosis formation in solid cancer.Coagulation and fibrinolysis factors take part in the metastasis progress of solid cancer. High concentration of u-PA and PAI-1 or low concentration of TFPI are considered to be related with poor outcome.

Objective To evaluate the clinical value of ”ligature anastomosis of the colon and anal canal” for middle-lower rectal cancer (M-LRC). Methods Restrospective analysis of the clinical data of 113 patients with M-L RC treated by ”ligature anastomoseof the colon and anal canal” with sustaining tube made by our self in recent seven years were made. Results Fifteen patients(13.2%) had postoperative complications.There was no death in this series.One hundred and eight patients were followed-up for 3 months to 8 years postoperatively. One hundred and one patients (93.5%) renewed anal function in 8 months to 1 year.Eight patients (7.4%)had local recurrence within 1 year postoperatively;15 patients (13.8%) had local recurrence 5 years after operation. Conclusions ”Ligature anastomosis of the colon and anal canal” after resection of the cancer in the treatment of M-LRC has successful effect in preserving anus. This operation is simple and less complications . It can be used nationwide.

A sensitive ABC or PAP immunohistochemical technique visualized with glucose oxidase-DAB-nickel method was used to examine the distribution and characteristics of the supraependymal serotoninergic nerve fibers (5-HT-SEP) in the adult rat forebrain. Three types of fibers could be distinguished: a few thick fibers (about 0.9?m in diameter), large amount of intermediate fibers (about 0.4?m in diameter), and numerous thin fibers (about 0.1?m in diameter). Although the 5-HT-SEP were found in all areas of the forebrain ependyma, the density and distribution patterns of different fiber types varied. In addition, the correspondence between the densities of ependymal cilia and 5-HT-SEP indicates the modulation of the cilia activity by 5-HT-SEP.

Objective To investigate the clinical characteristics, treatment and prevention methods of severe complications during hysteroscopic procedures Methods From January 1995 to January 2001, seventeen cases with uterine perforation, heavy bleeding and fluid overload respectively were encountered during hysteroscopic procedures This analysis focuses on their diagnosis and clinical management as well as prevention methods Results Three cases with uterine perforation and seven with uncomplete perforation followed transcerivcal resection of myoma (TCRM), transcerivcal resection of adhension, transcerivcal resection of septum and transcerivcal removal of foreign body respectively, which were diagnosied by both B ultrasonography and laparoscopy and treated laparoscopically Four cases with heavey bleeding were encountered during TCRM Foley catheter was inserted into uterine cavity to stop the bleeding successfully Three cases with fluid overload were cured by diuretic agent and saline infusion There were no serious consequence happened Conclusions It is a potential high risk factor of resulting in complications that performing complicated procedures inside uterine cavity during hysteroscopic operations Pre treatment with gonadotropin releasing a and guiding with B ultrasound/ laparoscopy as well as controlling irrigation pessure are necessary for proventing above complications

Objective To develop a method for the separation and preparation panaxynol. Methods Panax Notoginseng was extracted by SEF-CO_2, and absorbed by silica gel to remove most of the polar impurities. Panaxynol was preparated with RP-HPLC. Result Panaxynol was identified by UV、IR、MS、NMR,and the purity of panaxynol reached 98%. Conclusion The developed method is simple, reproducible and easy to operate. The solvent is cheap,with low boiling point and easy to recovery property.

Objective To analyze the effect of serum creatinine, blood urea and renal function with angiography low permeability of non-ionic contrast agent in elderly patients with ischemic cerebrovascular disease,and provide reference for clinical treatment.Methods 100 elderly patients with ischemic cerebrovascular disease in hospital from April 2011 to May 2016 were selected,patient were given cerebrovascular, renal arteriography.The changes of serum creatinine and urea were detected at different time before and after 3 days, and the changes of renal function were detected.Results In 100 cases, 12 cases of contrast induced nephropathy, the incidence rate was 12%.Contrast nephropathy in patients with diabetes mellitus group proportion, renal insufficiency, the proportion of severe renal artery stenosis was significantly higher than the proportion of non contrast nephropathy group (P<0.05).Contrast nephropathy group contrast 1 days and contrast 2 days blood urea, serum creatinine and creatinine clearance rate were significantly increased (P<0.05), contrast to 6 days gradually returned to normal, no obvious change of non contrast nephropathy in patients with renal function index.Diabetes mellitus (OR=6.973), severe renal artery stenosis (OR=4.286) and renal insufficiency (OR=7.680) are the risk factors of contrast induced nephropathy (P<0.05).Conclusion Vascular elderly patients with ischemic cerebrovascular disease using non-ionic contrast medium hypotonic angiography safer, diabetes, renal patients need to continue to pay attention to changes in renal function.

BACKGROUND: Studies have indicated that the abnormal expression of TACC3 is closely related to the occurrence and development of many kinds of tumors, and the expression of TACC3 is up-regulated in these tumors. Therefore, in vitro specific inhibition of TACC3 expression may become an important target for the treatment or intervention of tumor growth.OBJECTIVE: To investigate the mechanism by which TACC3 gene expression regulates cell proliferation and apoptosis in oral squamous cell carcinoma.METHODS: CD133+CD44+ oral squamous cell carcinoma cells were sorted from human oral squamous cell carcinoma cell line Cal-27 by immunomagnetic beads. In experimental group, the shRNA sequence of TACC3 was designed and synthesized, which was then trasnfected into CD133+CD44+ oral cancer stem cells by LipofectamineTM 2000. Empty vector-trasnfected (negative control) and untransfected cells were used as callsed. Forty-eight hours after the transfection, effects of TACC3 gene silencing on proliferation and apoptosis in vitro in CD133+CD44+ oral squamous cell carcinoma were detected by MTT, clone formation test, and TUNEL assay. Western blot assay was used to detect the effect of TACC3 gene silencing on Ki67, Bax and Bcl-2 protein expression in CD133+CD44+ oral squamous cell carcinoma.RESULTS AND CONCLUSION: (1) Cell proliferation. The proliferation rate and expression level of Ki67 were significantly lower in the experimental group than the negative control and untransfected groups (P < 0.05). (2) Clone formation. The clone formation ability in the experimental group was significantly lower than that in the negative control and untransfected groups (P < 0.05). (3) Cell apoptosis. TACC3 gene silencing caused an obvious decrease in Bcl-2 protein expression and a significant increase in Bax protein expression. These findings further confirmed that specific interference of TACC3 gene expression could inhibit the proliferation of CD133+CD44+ cells and promote the apoptosis.

Objective To study the effects of the pediatric sinusitis patients using furoic acid momethasone nasal spray after the treatment.Methods 60 pediatric sinusitis patients were divided into the two groups by the random number table method,gien the control group conventional treatment,and the observational group furoic acid momethasone,pediatric sinusitis nasal spray treatment,then the clinical effect and safety of the two groups of children were observe dand compared.Results After the treatment of the two groups,the observational group total effective rate(96.67%)was significantly higher than that of the control group (70.00%)(χ2 =10.23,P <0.05 ),the incidence of adverse reactions(3.33%)in children with significantly less than the control group(33.33%),which was statistically significant (χ2 =12.48,P <0.05 ).The observational group with bacterial clearance rate was significantly higher than that of the control group(90.91%)in children with bacterial clearance rate(61.90%), which was statistically significant(χ2 =13.26,P<0.05).Conclusion Using mometasone furoate nasal spray in the treatment of sinusitis in children can improve the total efficiency,and with low incidence of adverse reactions,which can effectively improve the clearance rate of bacteria,beneficial to restore the health of children,safe and effective and should be widely used in clinical practice.

Objective To compare the advantages and disadvantages of two methods for rat overactive bladder(OAB)model construction in?duced by intraperitoneal injection and intravesical instillation cyclophosphamide. Methods A total of 30 female SD rats weighting 200?250 g were randomly categorized into three groups:intraperitoneal injection?induced OAB(Ip?OAB),intravesical instillation?induced group(Iv?OAB)and con?trol group. Ip?OAB rats was i.p. administrated cycbophosphe mide three times in dose of 75 mg/kg body weight,while Iv?OAB rats received intravesi?cal instillation three times in drug dose of 75 mg/kg body weight. Control group rats received no treatment. Maximum bladder capacity(MBC),maxi?mum voiding pressure(MVP),frequency of spontaneous contraction of each group were recorded. The incidence,the mortality and the pathology of the three groups were compared. Results MBC,MVP and frequency of spontaneous contraction between Ip?OAB group and Iv?OAB group had no statistically significant difference(P>0.05). Compared with the control group,MBC significantly increased(P<0.05),MVP significantly decreased (P<0.05),and frequency of spontaneous contraction significantly increased(P<0.05)in Ip?OAB and Iv?OAB rats. The modeling success rate and mortality were 100%and 80%in Ip?OAB group,and were 50%and 0%in Iv?OAB group,and pathological changes were found in the two groups. Conclusion The construction of experimental animal model of OAB in rat induced by intraperitoneal injection and intravesical instillation cyclo?phosphamide are both reliable methods. Ip?OAB rats exhibit high incidence and mortality rate,while Iv?OAB rats show low incidence and mortality rate.