Objective To establish a reversed HPLC method to determine the contents of gatifloxacin and tinidazole simultaneously. Methods ODS C_ 18 column (4.6mm?250mm,5?m) was used as an analytical column. The mobile phase consisted of 15% acetonitrile, 25% of methanol and 60% of 0.05mol/L KH_2PO_4 buffer,which contained 0.5% triethylamine and was adjusted to pH 3.5 by phosphoric acid. The flow rate was 1ml/min. The detection wavelength was 318nm. The column temperature was 30℃. 20?l of diluted sample solution was injected to the sampler. Results The calibration curve of gatifloxacin was linear in the range of 2~20?g /ml (r=0.999 6) ,and that of tinidazole was 2~20 ?g /ml (r=0.999 9). The recovery rates of them were 100.1% and 100.4%,respectively. RSDs of both drugs were less than 1.1 %. Conclusions The method is simple,accurate and sensitive and can be used to determine the two components simultaneously.

Objective: To study the optimum particle size of Cordyceps sinensis for liver fibrosis in combination with in vitro dissolution experiment from serum pharmacology. Methods: To prepare the powder samples with different grinding degrees, Cordyceps sinensis was crushed through 100-, 150-, 200-, and 300-mesh sieves. The in vitro dissolution of adenosine was measured at different time points. Meanwhile, the powder sample was ig administered to rats, and pharmacodynamic approach was adopted to study the inhibition of medicated serum on HSC-T6 proliferation. Results: The accumulative in vitro dissolution of C. sinensis by 200-300 meshes was higher than that of other meshes. Medicated serum could significantly inhibit HSC-T6 cell proliferation. The AUC of HSC-T6 inhibition kinetics of medicated serum crushed to 200-300 meshes was significantly higher than that in other groups. Conclusion: The in vitro dissolution and pharmacodynamic method could be used for the study on different particle sizes of C. sinensis for anti-hepatic fibrosis, and 200-300 meshes are the optimal particle size.

Objective To discuss the resection technique for pancreatic carcinoma in patients with tumour(invasion) to the portal and/or superior mesentery vein,and the clinical significance of palliative resection of pancreatic carcinoma.Methods The clinical data of 71 cases of pancreatic carcinoma operated on within a period of 3 years were retrospectively reviewed.In patients with tumour invasion to the portal vein,a method of direc transection of pancreas was used in resection of the tumor,and was combined with local radiotherapy and chemotherapy.Results The resection rate was 57.75%.The complication rate was 22.54%.One patient died of multiple organ failure resulting from pneumonia.The survival rate at 6m,1-and 2-years was 100%,81.69%,and 40.85% respectively.Conclusions The direct transection method of pancreas(resection) is a simple and safe method to increase resection rate and prolong survival time for patients with(advanced) pancreatic carcinoma.

Objective To study the expression and significance of indoleamine-2,3-dioxygenase(IDO)in non-small-cell lung cancer(NSCLC).Methods Thirty-eight cases of lung cancer tissues and 10 cases of paracancerous normal lung tissue were collected.The semiquantitative immunohistochemistry was used to detect the positive rate of IDO and fluorescent quantitative PCR to detect the level of IDO mRNA in the lung tissues respectively.Results The positive rate of IDO and the level of IDO mRNA in NSCLC group were significantly higher than in paracancerous normal lung tissues(P＜0.05).There was no significant difference in the positive rate of IDO and the level of IDO mRNA between the adenocarcinoma group and the squamous cell carcinoma group(P＞0.05).Statistically,there was significant difference in positive rate of IDO and the level of IDO mRNA among low,middle and high differentiation groups(P＜0.05).There was significant difference in positive rate of IDO and the level of IDO mRNA between the lymph node metastasis and non-metastasis groups(P＜0.05).Conclusion The high expression of IDO in NSCLC is correlated with the degree of differentiation and the metastasis of lymph nodes,but not with the pathological types.

Objective To evaluate the value of spatio-temporal, image correlation (STIC) in prenatal diagnosis of complex heart malformations.Methods STIC was used to examine 25 fetuses with 20-30 weeks of gestation who were doubted with complex heart malformations prospectively by two-dimensional (2D) echocardiography.Dynamic three-dimensional imaging was achieved by postprocessing technique with STIC across the entire fetal heart and thorax.An offline analysis of the acquired volume was carried out to examine the fetal cardiac anatomy with multiplanar mode, surface rendering and minimum mode.The results of prenatal diagnosiswere compared with postnatal color Doppler and surgical findings.Results Successful STIC volume datasets were obtained in all 25 fetuses.The findings of all 25 fetus with complex heart malformations included double outlet of right ventricle in 1 case, truncus arteriosus in 1 case, complete transposition of the great arteries in 3 cases,corrected transposition of the great arteries in 1 case, complete endocardial cushion defect in 4 cases, hypoplastic right ventricle syndrome in 1 case, hypoplastic left heart syndrome in 2 cases, tricuspid atresia in 1 case,pulmonary atresia in 1 case, tetralogy of Fallot in 3 cases, single atrium in 1 case, single atrium and single ventrium in 4 cases,in 1 case, atrial septal defect and ventricular septal defect with coronary sinus dilatation in 1 case.The major diagnoses derived from STIC in 23 of 25 cases were consistent with the results of postnatal color Doppler and surgical findings.Conclusions STIC can provide more accurate information of structures of complex fetal heart malformations and has great value in pregnant prognosis.

Objective To summarize the experience of diagnosis and treatment of iatrogenic injury by ureteroscopic surgery.Methods Retrospective analysis of 13 cases with iatrogenic injury by ureteroscopy from December 2008 to December 2011,including 8 men and 3 women,aged 15 to 75 years.Among the 13 cases (Holmium laser lithotripsy under ureteroscope),there were 5 cases of ureterostoma severe disruption,4 cases of submucosa injury,2 cases of perforation,1 case of disruption,and 1 case of sleeve exfoliation of mucosa.Results Among these 13 cases with iatrogenic injury by ureteroscopy,10 cases underwent double J drainage (drainage duration:60 days),and 3 cases underwent open surgery immediately.There was no hydronephrosis when examined by IVU after six months to two years follow-up.Conclusions The skills and techniques of surgical operation should be improved when performing ureteroscopic operation,and it is essential to be familiar with ureteric dissection and alignment,which can avoid ureteric injury.Indwelling D-J tube is very important in dealing with mild ureteral injury secondary to ureteroscopes.Surgical intervention should be given to severe cases of ureteric injury in time.

Objective:To study the effects of the surgical treatment of nerve avulsion inside and outside the mandibular cannel.Methods:34 patients with trigeminal neuralgia of the third division,received neurotomy and avulsion of inferior alveolar nerve in the mandibular cannel above the lingual or through the mental foramen,concurrently with the avulsion of buccal nerve.Patients were fol-lowed up for 2-4 years.Results:All the operations were successfully completed,The wounds were well-healed.All patients felt that pain disappeared in the area innervated by inferior alveolar nerve.During the 2-4 years of follow-up,no recurrence was found.Conclusion:Inferior alveolar nerve neurotomy and avulsion in the mandibular cannel is simple,safe and effective in the treatment of trigeminal neuralgia of the third division.

By exploring the different components of the lysis buffer and optimize the 2-DE conditions,established the best proteomics technical system for Populus euphratica's heteromorphic leaves,while take the heteromorphic leaves in the same blanche as the test materials to find differences between the protein expressions of the leaves.It showed that the lysis solution which containing 2mmol/L thiourea,7mmol/L urea,2% CHAPS,60mmol/L DTT and 0.2% IPG buffer could dissolve the protein better.Through tandem mass spectrum,the results show that heteromorphic leaves are different in photosynthesis and respiration.This research offered valuable informations for understanding the molecular mechanism during leaves development and elucidating the mechanism of the eco-adaptability of Populus euphratica.

AIM: To investigate effect of Wujibaifeng Pills (WJBFP) on osteoporosis of ovariectonmized (OVX) rat. METHODS: Ovariectonmized (OVX) rat model was established to evaluate osteoporosis of which parameters investigated included bone gla protein (BGP), alkaline phosphatase (ALP), bone minera density (BMD), Serum phosphorus and serum total calcium. RESULTS: WJBFP(1.0g/kg,2.0g/kg,4.0g/kg) could enhance the contents of serum estradiol and calcitonin, decrease serum BGP level in OVX rats; It had no effect on serum total calcium and ALP activities but increase level of serum phosphorus; It could enhance BMD, prevent OVX rat from decreasing bone loss without raising body weight; furthermore, it could inhibit both the uterus and adrenal gland atrophy. CONCLUSION: WJBFP might have better prevention on osteoporosis of ovariectionmized rats.

Objective To evaluate the mental health of medical workers, and develop an EAP suitable for heahhcare sector. Methods A 1-year EAP was implemented in a hospital in Beijing, in which SF-36 and JSS scales were used to study the health-related quality of life and job satisfaction of these workers. Results Work pressure is found to be the main source of pressure for medical workers, accounting for 63.7%. Scores of the group receiving EAP service are found to be significantly higher (P<0.01) in all dimensions (excepting work nature and supervisor dimension) of both SF-36 and JSS. Conclusions Medical workers are exposed to high pressure of work, having strong need for EAP service as result. EAP is an effective means to maintain mental health and improve quality of life and job performance, if applied widely.