1.Immunoregulatory effects of mesenchymal stem cell and its application.
Xi-Ying LUAN ; Xue-Guang ZHANG
Acta Academiae Medicinae Sinicae 2006;28(3):448-452
The immunoregulatory effects of mescenchymal stem cell (MSC) and its application have become a hot research topic in recent years. This article reviews the up-to-dated research advances in the features and mechanisms of immune regulation of MSC and its application.
Animals
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Humans
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Lymphocyte Subsets
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immunology
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Mesenchymal Stem Cell Transplantation
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Mesenchymal Stromal Cells
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physiology
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T-Lymphocytes, Regulatory
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immunology
2.Experimental study on Rhizoma sparganii and Radices zedoariae treating hepatic fibrosis.
Zhu-ting XI ; Chang-min SHAN ; Xue-lian JIANG ; Xi-ying LUAN ; Ke-ke LI
China Journal of Chinese Materia Medica 2002;27(12):929-932
OBJECTIVETo study the effects of rhizoma sparganii and radices zedoariae on hepatic fibrosis.
METHODThe rat immunohepatic fibrosis model was made by intraperitoneal injection of porcine serum and treated with rhizoma sparganii and radices zedoariae. The ALT, GGT, TP, ALb, A/G, IVC, LN, HA and the pathological change of the liver were observed.
RESULTRhizoma sparganii and radices zedoariae could increase TP, ALb, A/G, decrease ALT, GGT, IVC, LN, HA and improve the pathological change.
CONCLUSIONRhizoma sparganii and radices zedoariae can protect hepatic cells, alleviate degeneration and necrosis, recover structure and function, and reduce the proliferation of fibrous tissue.
Animals ; Curcuma ; chemistry ; Drugs, Chinese Herbal ; isolation & purification ; therapeutic use ; Liver Cirrhosis ; drug therapy ; pathology ; Magnoliopsida ; chemistry ; Male ; Phytotherapy ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Rats ; Rats, Wistar ; Rhizome ; chemistry
3.The effects of B7H4 on human bone marrow mesenchymal stem cell inhibiting proliferation of PHA activated T cells.
Xi-Ying LUAN ; Wen-Zheng YU ; Qi-Zhi CAO ; Qiang FU ; Hai-Xia ZHANG
Chinese Journal of Hematology 2009;30(10):689-693
OBJECTIVETo investigate the effects of B7H4 on human bone marrow mesenchymal stem cells (HBMSC) mediating immune suppression.
METHODSThe expression of the negative immunoregulatory factor B7H4 on HBMSC were analyzed by RT-PCR and flow cytometry (FCM), respectively. The blocking experiment was used to detect the effects of B7H4 on HBMSC mediating suppression on PHA induced T cell activation, proliferation and cell cycle. HBMSC inhibiting T cell proliferation was examined by transwell cell culture system.
RESULTSB7H4 was highly expressed on HBMSC. Blocking the B7H4 expression by B7H4mAb significantly attenuated the inhibitory effects of HBMSC on T cell proliferation. Compared with that of the unblocking group, T cell stimulator index (SI) of the B7H4 blocked group was significantly increased (53 +/- 5 vs 15 +/- 8, P < 0.01) and the inhibitory effects of HBMSC on T cell cycle were weakened significantly through down-regulating the cell number in G(0)/G(1) phase \[(85.6 +/- 9.9)% vs (95.8 +/- 9.9)%\] and up-regulating those in S phase\[(5.8 +/- 3.2)% vs (2.3 +/- 2.2)%, P < 0.05\]. The suppressive effects of HBMSC on T cell proliferation were significantly weakened after separating HBMSC from T cells by transwell cell culture system. Compared with the cell to cell contact group, T cell SI was significantly increased (27 +/- 17 vs 15 +/- 3, P < 0.01).
CONCLUSIONHBMSC highly express B7H4, which plays an important role in the suppressive effects of HBMSC on T cell proliferation.
B7-1 Antigen ; metabolism ; physiology ; Bone Marrow Cells ; immunology ; metabolism ; Cell Cycle ; immunology ; Cell Proliferation ; Cells, Cultured ; Humans ; Lymphocyte Activation ; drug effects ; immunology ; Mesenchymal Stromal Cells ; immunology ; metabolism ; Phytohemagglutinins ; pharmacology ; T-Lymphocytes ; cytology ; drug effects ; immunology ; V-Set Domain-Containing T-Cell Activation Inhibitor 1
4.Cloning and expression of Streptococcus salivarius urease gene in Escherichia coli.
Yan WANG ; Xi-ping FENG ; You-hua XIE ; Dan-ying TAO ; Xiao-ling LUAN
Chinese Journal of Stomatology 2010;45(8):498-501
OBJECTIVETo clone Streptococcus salivarius (Ss) 57. I urease gene, which can express ureolytic activity in Escherichia coli (Ec) without adding extra nickel ions.
METHODSUrease gene was cloned by polymerase chain reaction in three separate parts. The three separate plasmids were digested by specific restriction enzymes and ligated together. The expression of the complete urease gene in Ec was detected by phenol red assay and pH analysis.
RESULTSUrease gene of Ss 57.I was eventually cloned and proved correct. Urease activity of the obtained clone was positive in Ec. Without adding extra NiCl(2), the recombinant Ec could hydrolyze urea to produce ammonia, resulting in the increase of pH value.
CONCLUSIONSThe clone of Ss urease gene obtained in this study could express ureolytic activity in Ec without adding extra nickel ions. The current clone can be used to construct ureolytic effector strain used in replacement therapy in caries prevention.
Cloning, Organism ; Dental Caries ; microbiology ; prevention & control ; Escherichia coli ; genetics ; Hydrogen-Ion Concentration ; Nickel ; chemistry ; Streptococcus ; genetics ; Urease ; genetics
5.Inhibition of TAK1 aggravates airway inflammation by increasing RIPK1 activity and promoting macrophage death in a mouse model of toluene diisocyanate-induced asthma.
Shu Luan YANG ; Wen Qu ZHAO ; Xian Ru PENG ; Zi Han LAN ; Jun Wen HUANG ; Hui Shan HAN ; Ying CHEN ; Shao Xi CAI ; Hai Jin ZHAO
Journal of Southern Medical University 2022;42(2):181-189
OBJECTIVE:
To explore the effect of transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1) on toluene diisocyanate (TDI)-induced allergic airway inflammation in mice.
METHODS:
Thirty-two mice were randomly divided into AOO group, AOO+5Z-7-Oxozeaenol group, TDI group, and TDI+5Z-7-Oxozeaenol group. Another 32 mice were randomly divided into AOO group, TDI group, TDI +5Z-7-Oxozeaenol group, and TDI +5Z-7-Oxozeaenol + Necrostatin-1 group. TAK1 inhibitor (5Z-7-Oxozeaenol, 5 mg/kg) and/or RIPK1 inhibitor (Necrostatin-1, 5 mg/kg) were used before each challenge. Airway responsiveness, airway inflammation and airway remodeling were assessed after the treatments. We also examined the effect of TDI-human serum albumin (TDI-HSA) conjugate combined with TAK1 inhibitor on the viability of mouse mononuclear macrophages (RAW264.7) using CCK8 assay. The expressions of TAK1, mitogen-activated protein kinase (MAPK) and receptor interacting serine/threonine protease 1 (RIPK1) signal pathway in the treated cells were detected with Western blotting. The effects of RIPK1 inhibitor on the viability of RAW264.7 cells and airway inflammation of the mouse models of TDI-induced asthma were evaluated.
RESULTS:
TAK1 inhibitor aggravated TDI-induced airway inflammation, airway hyper responsiveness and airway remodeling in the mouse models (P < 0.05). Treatment with TAK1 inhibitor significantly decreased the viability of RAW264.7 cells, which was further decreased by co-treatment with TDI-HSA (P < 0.05). TAK1 inhibitor significantly decreased the level of TAK1 phosphorylation and activation of MAPK signal pathway induced by TDI-HSA (P < 0.05). Co-treatment with TAK1 inhibitor and TDI-HSA obviously increased the level of RIPK1 phosphorylation and caused persistent activation of caspase 8 (P < 0.05). RIPK1 inhibitor significantly inhibited the reduction of cell viability caused by TAK1 inhibitor and TDI-HSA (P < 0.05) and alleviated the aggravation of airway inflammation induced by TAK1 inhibitors in TDI-induced mouse models (P < 0.05).
CONCLUSION
Inhibition of TAK1 aggravates TDI-induced airway inflammation and hyperresponsiveness and may increase the death of macrophages by enhancing the activity of RIPK1 and causing persistent activation of caspase 8.
Animals
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Asthma/chemically induced*
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Inflammation
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Macrophages
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Mice
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Receptor-Interacting Protein Serine-Threonine Kinases
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Respiratory System
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Toluene 2,4-Diisocyanate/adverse effects*