BACKGROUND:Self-assembling peptide scaffolds have attracted much more attention among three dimensional biomaterials due to its biocompatibility, biodegradation, and tailor-made properties.
OBJECTIVE:To review the structure and design of functionalized self-assembling RADA peptide, and the recent advances in the use of RADA self-assembling peptide for three-dimensional cel culture in cel therapy applications.
METHODS:Total 224 literatures related to self-assembling peptide and tissue engineering from PubMed, Web of Science and CNKI databases were screened out for this review. The keywords were“self-assembly peptide, tissue engineering”in English and Chinese, respectively. Final y 48 of 224 articles about the design, fictionalization, and three-dimensional cel culture of peptide scaffolds were included in this review.
RESULTS AND CONCLUSION:Self-assembling peptide could undergo spontaneous assembly into wel-ordered interwoven nanofibers in water and rapidly form hydrogel, which physical y mimics the architecture of natural extracel ular matrix to ensure a real three-dimensional microenvironment for cel s. In terms of bio-function, this material can be tailor-made with various bioactive short peptide motifs to promote cel adhesion, proliferation, and differentiation.

Adult ; Drug Resistance, Viral ; genetics ; Female ; Genotype ; Guanine ; analogs & derivatives ; pharmacology ; therapeutic use ; Hepatitis B virus ; drug effects ; genetics ; Hepatitis B, Chronic ; drug therapy ; genetics ; Humans

Objective To investigate the condition of the professional identity and learning burnout in the college students majored in re-habilitation therapy, and discuss the correlation between them. Methods From October to November, 2014, students of grades 2 and 3 ma-jored in rehabilitation therapy were investigated with the professional identity questionnaire and the learning burnout questionnaire. Results A total of 127 questionnaires were sent out, and 119 returned. The score of professional identity was (3.36 ± 0.55), with the factors score of the cognition, emotion, behavior, appropriateness as (3.66±0.77), (3.25±0.60), (3.23±0.71), and (3.36±0.61), respectively. The score of learn-ing burnout was (2.90±0.46), the score of factors of low spirits, improper behavior and low achievement as (2.87±0.70), (3.10±0.60), (2.77± 0.52), respectively. There was negative correlation between score of professional identity and learning burnout (r=-0.271, P<0.01), as well as between some factors of the identity and the learning burnout (P<0.05). Conclusion The professional identity and learning burnout are in the middle level in students majored in rehabilitation therapy, which may interact each other.

The antibacterial property of silver nanoparticles has resulted in their widespread application in many fields,so the chance of silver nanoparticles exposure for human increased greatly.Thus,there is urgent need to assess the safety of such particle.So far,most toxicological studies of silver nanoparticles mainly focus on the cytotoxicity using different examination endpoint such as morphology,mitochondrial function,cell proliferation,enzyme activity,and so on.In addition,the in vitro studies on the toxicity of silver nanopoarticles are also reported,few of the study on molecule mechanism of toxicity was reported.This review provided a summary of antibacterial mechanism of silver nanoparticles and the current research situation of the safety.The future research direction of toxicological study of silver nanoparticles is also prospected based on the current knowledge.

Objective To find a sensitive index and to investigate the working memory impairment of patients with amnestic mild cognitive impairment.Methods Fifteen patients with amnestic mild cognitive impairment,15 healthy matching aging controls were performed a matching-to-sample task while event-related potential(ERP),reaction time and correct rate were recorded.Subjects were required to press a button in the match condition and another button in the conflict condition.Results In the matching condition,there was no difference between the two groups in distribution(parietal lobe),peak latency and amplitude of P300(F_ 1,28 =1.0324,P=0.3183;F_ 2,42 =0.543,P=0.585).In the conflict condition,the distribution of N270 was fore head,and its latency of patient group were more delayed than the aging group(F_ 1,28 =25.3264,P=0.000),but its amplitude showed no significant changes(F_ 1,28 =0.507,P=0.482).The result of brain mapping showed same change.Conclusion The N270 component is more sensitive than P300 to reflect the central executive function impairment of working memory in patients with amnestic mild cognitive impairment.

OBJECTIVE:To establish a method for simultaneous determination of camphor and menthol in Compound diphen-hydramine liniment. METHODS:GC was performed on the column of Agilent 19095N-123 INNOWAX capillary column,column temperature was 120 ℃,injection volume temperature was 220 ℃,FID detector was used with the temperature of 260 ℃,carrier gas was nitrogen gas,flow of column was 6.0 ml/min,air was 450 ml/min,hydrogen gas was 40 ml/min,split ratio was 20∶1 and injection volume was 1 μl. RESULTS:The linear range of mass concentration was 0.612-6.12 mg/ml(r=0.999 9)for camphor and 0.593-5.93 mg/ml(r=0.999 9)for menthol;RSDs of precision,stability and reproducibility tests were lower than 2%;the recov-eries were respectively 98.0%-100.7%(RSD=1.0%,n=6)and 98.7%-100.7%(RSD=0.7%,n=6). CONCLUSIONS:The meth-od is simple,accurate and reproducible,and can be used for the contents determination of camphor and menthol in Compound di-phenhydramine liniment.

Objective:To analyze the relationship between corrected Tp-e interval(Tp-ec), Tp-e/QT ratio and the degree of vein bridge vessel stenosis after coronary artery bypass grafting(CABG) in patients with left main coronary artery stenosis, and its predictive value for malignant electrophysiological events.Methods:Patients admitted to Beijing Anzhen Hospital affiliated to Capital Medical University from January 2018 to August 2019 were selected, coronary angiography was performed 5-7 years after coronary artery bypass grafting(CABG) due to recurrence of symptoms. 169 patients with >50% restenosis in SVG were selected. According to the degree of bridge angiography stenosis, the patients were divided into group A(50%-70% SVG restenosis, 105 cases) and group B(SVG restenosis >70%, 64 cases), routine electrocardiogram examination, measurement and correction of QT interval(QTc), Tp-ec and Tp-e/QT ratio were made. Patients were followed up 6 months, the occurrence of malignant ECG events is observed, and the area under the receiver operating characteristic( ROC) curve( AUC) was used to quantitatively evaluate the predictive power of Tp-ec and Tp-e/QT ratios for malignant ECG events. Results:The QTc, Tp-ec and Tp-e/QT ratios in group B were significantly greater than those in group A, and the differences were statistically significant( P<0.05). After multivariate logistic regression analysis, both the Tp-ec and Tp-e/QT ratios were independent influencing factors for the degree of vein bridge vessel stenosis>70%( P<0.05). After Pearson correlation analysis, both the Tp-ec and Tp-e/QT ratios were positively correlated with the degree of vein bridge vessel stenosis( r= 0.384 and 0.457, both P=0.000). A total of 162 cases(95.86%) were followed up, 25 cases of malignant electrophysiological events occurred. After ROC curve analysis, Tp-ec combined with Tp-e/QT ratio predicted coronary malignant electrophysiological events in patients with left main artery stenosis after GABG with AUC=0.924, which was significantly greater than 0.698 of the single index Tp-ec and 0.712 of the Tp-e/QT ratio, the differences were both statistically significant( P<0.05). Conclusion:Tp-ec and Tp-e/QT ratios are both positively correlated with the degree of vein bridge vessel stenosis after CABG in patients with left main coronary artery stenosis, the combined effect of them in predicting the occurrence of malignant electrocardiological events is good, and it is worth further research and application.

Objective To investigate the establishment of rabbit model of obstructive sleep apnea syndrome(OSAS) through hypobaric hypoxia and their changes in pathophysiology.Methods Twelve adult rabbits were randomly assigned to 2 groups,control group and experimental group.The rabbits of experimental group were placed in hypobaric hypoxia cabin,in which the altitude was 5 000 m,10 h daily,for 4 weeks.The rabbits of control group were raised normally.The airway were scanned by CT,arterial blood gas was analyzed and respiratory airflow was monitored.The rabbits were sacrificed after the experiment,and pharyngeal tissue and genioglossus were harvested for pathological examination.Results In hyoid bone plane of upper airway,the pharyngeal of experimental group showed significant thickened(P

Objective To analyze clinicopathologic characteristics and prognostic factors of the patients with invasive lobular carcinoma of breast. Methods Clinical data of 125 patients with invasive lobular carcinoma of breast treated at Cancer Center of Sun Yat-sen University from Jan. 1990 to Dec. 2008, were analyzed. The clinical characteristics, recurrence and survival of the patients were summarized. Results Median age of 125 patients was 45 years old (range, 27 to 76 years old). The patients with large tumor mass (≥ 3cm), positive local lymph node, more than Ⅱ stage and positive hormone receptor at diagnosis were 77 cases(61.6 %), 64 cases(51.2 %), 101 cases(80.8 %) and 112 cases(89.6 %), respectively. The median time of follow-up was 58 months (range, 11-222 months). Of the 125 patients, 32 had local recurrence and metastasis, and 18 died. The 5-year disease-free and overall survival rates were 82.2 % and 87.3 %, respectively. Multivariate COX regression analysis showed that whether endocrine therapy or not was only a prognostic factor of patients with invasive lobular carcinoma of breast. Conclusion There is no difference in media age of patients with invasive lobular carcinoma of breast at diagnosis from other pathologic type of breast cancer. These patients are usually with larger tumor masses, more lymph node metastasis and a higher proportion of positive hormone receptor. The prognosis of patients is not affected by clinicopathologic parameters.

Objective To investigate the relationship between diabetes and bone metabolism and the potential epigenetic mechanisms. Methods BMSCs were cultured for 7 and 15 days in cell culture medium with different concentrations of glucose. The mRNA and protein expression of HDACs and osteogenesis-related genes were detected by RT-PCR and Western blot assay ,respectively. Moreover ,the combination of HDAC to the promoter region of Runx2 was tested by the chromatin immunoprecipitation (ChIP) assay. Results ThemRNA expression of osteogenesis-related genes ,incuding OCN(P < 0.05)and Col1(P < 0.05),in the bone marrow of diabetic mice was significantly reduced compared with the control mice. The mRNA and protein expression of ALP ,OCN ,Runx2 and OSX was gradually reduced with the increasing concentration of glucose ,while HDAC2 mRNA and protein expression was increased. The binding activity of HDAC2 to the upstream and downstream of Runx2 promoter region in 25mM glucose-treated BMSCs was higher than the control group(P < 0.05). Conclusoins Diabetes might repress osteogenesis of BMSCs via inhibiting the activity of Runx2 through upregu-lating the expression of HDAC2.