1.Expression of BSAP/CD30 in classic Hodgkin lymphoma using double-staining technique.
Yan-Feng XI ; Wen-Qi BAI ; Jin-Fen WANG ; Quan-Hong WANG ; Shi-Lan JIAO
Chinese Journal of Pathology 2007;36(2):136-137
Adolescent
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Adult
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Aged
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Biomarkers, Tumor
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metabolism
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Child
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Female
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Gene Expression Regulation, Neoplastic
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Hodgkin Disease
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genetics
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metabolism
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Humans
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Ki-1 Antigen
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metabolism
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Male
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Middle Aged
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PAX5 Transcription Factor
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metabolism
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Staining and Labeling
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methods
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Young Adult
2.Clinical analysis of morphine hydrochloride sustained-release tablets combined with celecoxib in the treatment of advanced lung cancer with moderate to severe cancer-induced pain
Xueqin LI ; Xi CHEN ; Quan GONG ; Wei SHI ; Lijuan ZHANG ; Hui DONG ; Cunde WANG
Journal of International Oncology 2017;44(3):165-168
Objective To explore the efficacy,safety and life quality of patients of morphine hydrochloride sustained-release tablets combined with celecoxib in the treatment of advanced lung cancer with moderate to severe cancer-induced pain.Methods A total of 247 patients of advanced lung cancer with moderate to severe cancer-induced pain were randomly divided into combination therapy group (n =127) and morphine monotherapy group (n =120) using simple random sampling digital table method.The differences of dose,efficacy,adverse drug reactions and life quality between the two groups were analyzed.Results In achieving similar analgesic effect,the average maintenance dose of morphine in combination therapy group was (52.51 ±19.92)mg/d,lower than that in monotherapy group [(58.75 ±20.64)mg/d,t =-2.414,P =0.017].The incidence of constipation in combination therapy group was 34.6%,lower than that in monotherapy treatment group (47.5 %,x2 =4.218,P =0.040).The life quality of the two groups were improved,and the life quality improvement rate in combination therapy group was 59.8%,higher than that in monotherapy treatment group (43.3%,x2 =6.736,P =0.009).Conclusion Morphine hydrochloride sustained-release tablets combined with celecoxib is effective in the treatment of moderate to severe cancer pain,which can reduce the dosage of morphine and reduce adverse reaction,so as to improve the life quality of the patients with advanced lung cancer.
3.Repair of large area of tracheal wall defects with silastic framework:an experimental study
Si-Quan TANG ; Dai-Cheng LIN ; Shi-Xi LIU ; Long-Yue LIU ; Tian-Ming ZHOU ;
Chinese Journal of Trauma 2003;0(07):-
Objective To explore the feasibility of artificial silastic framework(SF)in repair of large area of tracheal wall defects.Methods Twenty healthy adult dogs with tracheal defects for 2.5 cm?6.0 cm-3.0 cm?6.0cm were randomly and equally divided into experimental group(repaired with SF combined with sternohyoid fasciae)and control group(repaired with T-silastie tubule combined with sternohyoid fascial flap).After the operation,the animals were sacrificed at the 4th,8th,16th,24th, and 48th weeks respectively for harvesting the tracheae that were used for tracbeoscopically observing in- flammatory reaction of the repaired defect area and light microscopically observing epithelium healing on the repaired defect area.Results In the experiment group,the repaired trachea was smooth,without proliferation of granulation;and at the 8th week,the repaired defect area was covered with epithelial cells,with good functional recovery of respiration,phonation and deglutition.In the control group,there was obvious proliferation of granulation on the tracheal surface near anterior and posterior ends of T-silas tic tubule.The animals were under asphyxia to die with extraction of T-silastic tubule.Conclusions SF has excellent tracheal skeletal function.In the meantime,SF combined with sternohyoid fasciae is a simple but effective method for repair of large area of tracheal wall defects.
4.Wild-tending techniques study on Glycyrrhiza uralensis--effect of irrigation and rhizome length on survival ratio, yield and quality.
Li LI ; Sheng-Li WEI ; Wen-Quan WANG ; Hua SHI ; Sogumeyi TODA ; Zheng-Zheng GUO ; Guang-Xi REN
China Journal of Chinese Materia Medica 2014;39(15):2863-2867
This research aimed at studying the effects of irrigation and rhizome length on the survival of ratio, yield and quality of Glycyrrhiza uralensis in wild tending condition. Employed the split-block design to carry out the field experiment, sampled with the quadrat method to measured the relative growth indexes and to estimate the yield, used the HPLC (high performance liquid chromatog- raphy ) method to measure the glycyrrhizin in the rhizome and adventitious root of the G. uralensis in this study. The quantity of the adventitious roots and the survival ratio were increased significantly as the length of the rhizome increased (P < 0.01), but the length of the rhizome had no remarkable effect on the content of glycyrrhizin. The average content of the glycyrrhizin in the adventitious root and rhizome could reach 3.03% and 2.12% after 3-year wild tending, respectively, and this results indicated that the quality of the glycyrrhiza using this method was much better than that from cultured glycyrrhiza with the reproducing method of seeding. so using the rhizome as reproductive material to produce the glycyrrhiza under the wild tending condition could get the high quality glycyrrhiza quick- ly and steadily, this phenomenon could be explained by the Hypothesis of synthetic inertia of the medicinal components from the wild material of G. uralensis. But the maximum yield with this method was just more than 945 kg x hm(-2) in this study. So the further work of how to increase the yield in the practical application with the method found in this study need to be done in the next research.
Agricultural Irrigation
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Culture Techniques
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methods
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Glycyrrhiza uralensis
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growth & development
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metabolism
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Glycyrrhizic Acid
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metabolism
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Rhizome
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growth & development
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Survival Analysis
5.Inflammatory reaction changes with aging in kidneys of human TIMP-1 transgenic mice
Xue-Guang ZHANG ; Xiang-Mei CHEN ; Quan HONG ; Xi-Yao SHANG ; Suo-Zhu SHI ; Zhong YIN ; Guang-Yan CAI
Chinese Journal of Geriatrics 2003;0(12):-
Objective To explore the role of tissue inhibitor of metalloproteinase-1(TIMP-1) during renal senescence by using human TIMP-1 transgenic mice.Methods Renal histological changes of wild type mice and transgenic mice at the age of 3,12,24 months were observed by periodic acid-schiff(PAS)staining of paraffin sections.The numbers of F4/80 positive cells were detected by immunofluoreseence.The protein expressions of TIMP-1,TIMP-2,matrix metalloproteinase(MMP)-9,MMP-2,intercellular adhesion molecule-1(ICAM-1),transforming growth factor?1(TGF-?1),collagenⅢand collagenⅣwere detected by Western blot.The activities of gelatinases and TIMP-1 were examined by gelatin zymography and reverse zymography respectively.Results Focal renal fibrosis was found in two genotypes with aging.At the age of 24 months,compared with wild type,in kidneys of transgenic type,the expressions and activities of gelatinases were dowregulated (MMP-2:2.08?0.20 vs.3.39?0.43;MMP-9:4.02?0.82 vs.6.72?1.40,all P<0.05);the expressions of collagenⅢ,collagenⅣ,ICAM-1,and TGF-?1 were upragulated(0.72+0.11 vs.0.57?0.09;0.84?0.13 vs.0.6?0.11,0.72?0.12 vs.0.53?0.07; 0.69?0.12 vs.0.45?0.09,all P<0.05),and the numbers of F4/80 positive cells were increased (18.8?4.4 vs.12.7?3.6,P<0.05)with the upregulated expression and activity of TIMP-1(1.10?0.18 vs.0.62?0.09;50.75?7.25 vs.20.64?3.50,P<0.05).Conclusions TIMP-1 could promote age-related renal fibrosis through enhancing inflammation reaction by ICAM-1 upregulation.
6.Specific expression of beta-actin during spermatogenesis in rats.
Yi-ming CHENG ; Xi-quan SHI ; He-ming YU ; Yan-wan WU ; Meng-chun JIA
National Journal of Andrology 2005;11(10):755-760
OBJECTIVETo screen the stage-specific expression proteins during rats spermatogenesis, and to investigate the beta-actin expression and localization in the tissues of rat testicular.
METHODSHighly enriched type A spermatogonia, pachytene spermatocytes and round spermatids were isolated by STAPUT method (sedimentation velocity at unit gravity, with 2% - 4% BSA gradient in DMEM/F12 medium) respectively to get the total proteins. The difference of protein expression between the three kinds of cells was analyzed by two-dimensional electrophoresis. Then the distribution of beta-actin in rat testicular tissues was investigated using specific anti-beta-actin antibodies by immunohistochemical method.
RESULTSbeta-actin was identified as a stage-specific expression protein by two-dimensional electrophoresis. beta-actin protein was more strongly expressed in type A spermatogonia and pachytene spermatocytes, but not in round spermatids. The immunohistochemical results showed that beta-actin was mainly located in the cytoplasm of type A spermatogonia and pachytene spermatocytes and in the nuclei of nearly mature spermatids.
CONCLUSIONbeta-actin protein is a stage-specific expressed protein and may play an important role in spermatogenesis.
Actins ; biosynthesis ; Animals ; Electrophoresis, Gel, Two-Dimensional ; Male ; Mass Spectrometry ; Rats ; Rats, Sprague-Dawley ; Spermatogenesis ; physiology ; Testis ; cytology ; metabolism
7.Expression of brain-derived neurotrophic factor at acute inflammatory injury of the brain.
Ling LI ; Quan-xiang SHUI ; Xi-lin YU ; Shi-qiang SHANG ; Wei-zhong GU ; Hong-feng TANG
Journal of Zhejiang University. Medical sciences 2003;32(5):433-436
OBJECTIVETo investigate the expression of brain-derived neurotrophic factor (BDNF) mRNA and immunoreactivity in experimental acute inflammatory brain injury.
METHODSTen rats were inoculated with pneumococcus to establish the model of bacterial inflammatory brain injury and other 6 rats were used as normal controls. At 24 h after inoculating, the expression of BDNF mRNA and BDNF protein in brain tissue was detected by in situ hybridization and immunohistochemical methods, respectively.
RESULTThe necrosis of neuron in cerebral cortex and hippocampus was observed after infection. The increase of BDNF mRNA expression in the cerebral cortex and hippocampus of experimental animals was demonstrated at 24 h after inoculation: (0.1194 +/- 0.02941 compared with 0.0662 +/- 0.01176)A and (0.1608 +/-0.01854 compared with 0.0680 +/- 0.00946)A (P<0.01), respectively. Compared with controls the expression of BDNF protein in the cerebral cortex and hippocampus was enhanced at 24 h of inoculation:(177.04+/-43.66 compared with 79.79+/-7.23)mm(2) (P<0.01) and (81.78 +/-37.47 compared with 42.98 +/-20.44)mm(2) (P<0.01), respectively. Strong positive hybridization and immunoreactivity were observed in the infiltrated inflammatory cell in leptomeninges, subarachnoid cavity, ventricles and brain parenchyma in the brain from the experimental rats.
CONCLUSIONThe expression of BDNF mRNA and BDNF protein increases following brain inflammatory injury, which supports the hypothesis that BDNF may constitute intrinsic neuroprotective mechanism as a part of the inflammatory response.
Acute Disease ; Animals ; Brain-Derived Neurotrophic Factor ; analysis ; genetics ; Calcium ; metabolism ; Female ; Immunohistochemistry ; Male ; Meningitis, Pneumococcal ; metabolism ; RNA, Messenger ; analysis ; Rats ; Rats, Sprague-Dawley
8.Isolation, culture and purification of olfactory ensheathing cells from human fetal olfactory mucosa.
Qiang LI ; Xi-Jing HE ; Jian-Feng SHI ; Bin WANG ; Zhen-Zhong ZHU ; Quan-Jin ZANG ; Pei FAN
Journal of Southern Medical University 2008;28(11):1974-1976
OBJECTIVETo explore the method for obtaining olfactory ensheathing cells from human fetal olfactory mucosa by cell culture for selective adhesion in the presence of neurotrophin-3 (NT3) and low-concentration serum.
METHODSThe olfactory ensheathing cells were cultured alternatively in DMEM/F12 culture medium containing 10% fetal bovine serum (FBS) and the medium containing NT3 and 2.5% FBS every 72 h. The cells were observed for morphological changes and identified using immunocytochemistry with P75NTR and GFAP, and the cell purity was estimated.
RESULTSThe olfactory ensheathing cells from human fetal olfactory mucosa were positive for P75(NTR) and GFAP, and in in vitro culture, the cells exhibited dipolar or tripolar appearance with long thin neurites. On the 9th day of cell culture, the purity of the olfactory ensheathing cells reached about 83%.
CONCLUSIONThe olfactory ensheathing cells can be obtained by in vitro culture for selective adhesion in the presence of NT3 and low-concentration serum.
Cell Culture Techniques ; methods ; Cell Separation ; methods ; Cells, Cultured ; Culture Media ; Fetus ; Humans ; Neurotrophin 3 ; pharmacology ; Olfactory Bulb ; cytology ; Olfactory Mucosa ; cytology
9.Management of difficult, severe and recurrent Budd-Chiari syndrome.
Zhong-gao WANG ; Chun-min LI ; Yong-quan GU ; Heng-xi YU ; Bing CHEN ; Lian-rui GUO ; Xue-feng LI ; Shi-jun CUI ; Zhen LI
Chinese Journal of Surgery 2008;46(15):1149-1152
OBJECTIVETo investigate the management of complicated, severe or recurrent Budd-Chiari syndrome.
METHODSFrom February 2004 to August 2007, 28 patients with complicated, severe or recurrent Budd-Chiari syndrome were treated. In this series, 16 patients relapsed after treated with percutaneous transluminal angioplasty or stent deployment, 2 cases relapsed after surgery; and the other 10 were under severe conditions and hard to treat, including malignancy of the inferior vena cava and right atrium. Meso-cavo-atrial shunt was carried out in 10 cases, meso-cavo-jugular shunt in 6 (capitis medusa was used in one case), cavoatrial shunt in 2 and cavo-jugular shunt in 1, mesocaval shunt in 2, and radical or extended radical correction in 7.
RESULTSOne patient (3.6%) died in 24 hours after operation. Graft infection occurred in 1 case. Excellent, good, fair, poor and death rate were 22.2%, 55.5%, 14.8%, 3.7% and 3.7%, respectively, the overall effective rate was 92.5%.
CONCLUSIONTo select personalized treatment according to the disease status brings hopes to difficult, severe, recurrent Budd-Chiari syndrome.
Adolescent ; Adult ; Blood Vessel Prosthesis Implantation ; Budd-Chiari Syndrome ; surgery ; Child ; Child, Preschool ; Critical Illness ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Portacaval Shunt, Surgical ; Recurrence ; Retrospective Studies ; Treatment Outcome
10.Risk factors of delayed cerebral vasospnsm following subarachnoid hemorrhage: a clinical analysis
Jin-Fu FENG ; Chuan-Zhi DUAN ; Xi-Feng LI ; Zhi-Qiang FA ; Yi-Quan KE ; Shi-Zhong ZHANG ; Xiao-Dan JIANG
Chinese Journal of Neuromedicine 2009;8(5):509-512
Objective To identify the risk factors of delayed cerebral vasospasm following aneurysmal subarachnoid hemorrhage (SAH). Methods The clinical data of 74 patients with SAH were retrospectively analyzed. The severity of delayed cerebral vasospasm in these patients was assessed according to the findings by digital subtraction angiography, and the risk factors for delayed cerebral vasospasm following SAH were analyzed. Results The patients' age, number of SAH episode, histor yof smoking, Hunt-Hess grade, Fisher grade and peak white blood cell count were found to significantl ycorrelated to the occurrence of delayed cerebral vasospasm. Among these factors, the patients' age, number of SAH, Hunt-Hess grade, and Fisher grade were identified as the independent risk factors of delayed cerebral vasospasm. Conclusions Patients with younger age, SAH more than twice, and Hunt-Hess or Fisher grades over Ⅲ are at higher risk of delayed cerebral vasospasm. Close monitoring fo rearly detection of delayed cerebral vasospasm and timely management are crucial in these patients.