2.Analysis of the lymphocyte subsets in cerebrospinal fluid in patients with HIV infection
Xi QIAO ; Jinlan SHEN ; Ping ZENG ; Tong LIU ; Yanyan WANG
International Journal of Laboratory Medicine 2015;(8):1052-1054
Objective To explore the lymphcyte subsets distribution in cerebrospinal fluid in patients with HIV infection ,and to investigate the clinical significance of the lymphocyte subsets in cerebrospinal fluid in HIV central nervous system complication . Methods 34 patients with HIV infection ,including 20 patients without nervous system symptoms (simple HIV group) and 14 pa‐tients with nervous system symptoms (neurological HIV group) ,and 15 cases of healthy people (control group) were selected . Flow cytometry was used to detect lymphocyte subsets ,and immunoturbidimetry was used to detect the level of IgG in cerebrospinal fluid .Results The percentage of CD8+ T cells was higher and percentage of CD4+ T cells was lower in the simple HIV group and neurological HIV group than those in the healthy control ,with statistically significant differences (P<0 .01) .The level of IgG in pa‐tients with HIV infection was higher than that in the healthy control group (P<0 .01) .While no significant difference were found in the percentage of B cells and NK cells among the there group (P>0 .05) .There were also no significant differece between the sim‐ple HIV group and neurological HIV group in the ratio of each lymphcyte subset in cerebrospinal fluid (P>0 .05) .Conclusion The immune disorder in cerebrospinal fluid in patients with HIV infection may appear in the early time before the nervous system com‐plication .The changing trends of lymphocyte subsets are consistent with the peripheral blood ,which demonstrate that the T lym‐phocyte subsets may be correlated with the nervous system symptoms of HIV .
3.Influence of the excision of cardiac sympathetic nerves on QT dispersion
Huiyun ZHOU ; Shengxi ZHEN ; Xi LAN ; Yu LIU ; Ping SHU
Chinese Journal of Pathophysiology 2000;0(07):-
AIM: To observe and study the influence of cardiac sympathetic nerve on QT dispersion (QTd) and the circadian variations of QTd in experimental rabbits. METHODS: The rabbits were divided into experimental group (without cardiac sympathetic control by operation) and control group (with retained cardiac sympathetic control by operation, sham operation). QTd of both groups were measured and compared before and after the operation. The circadian variations of QTd were also observed in both groups. RESULTS: QTd in experimental group decreased significantly after the cardiac sympathetic nerves were excised (P
4.Multi-slice spiral CT angiography of the posterior nutcracker syndrome
Long ZHAO ; Heshui SHI ; Xi LIU ; Ping HAN ; Jie YU
Chinese Journal of Medical Imaging Technology 2009;25(10):1817-1820
Objective To assess the value of multi-slice spiral CT angiography (MSCTA) in diagnosis of posterior nutcracker syndrome (NCS). Methods MSCTA of 15 patients clinically diagnosed as posterior NCS (patient group) and 50 subjects with normal renal vessels (control group) were retrospectively analyzed. The anatomy, course and relationship to the adjacent structure of left renal vein (LRV) and its branches were observed. The anteroposterior diameter (d1) and cross areas (s1) of the angle of control group and posterior abdominal artery (AA), the anteroposterior diameter (d2) and cross areas (s2) at the largest lumen near the renal hilar were measured and the ratio of s2/s1 and d2/ d1 were calculated.Results All LRVs posterior AA of patient group were compressed and narrowing with dilating apparently near the renal hilar. The difference of d2 was not significant (P>0.05), but of other values were significant (P<0.05).Conclusion MSCTA can show anatomy and three-dimentional trend clearly of LRV for posterior NCS, and evaluate the extent of narrow and dilating lumen accurately with cross areas measuring.
6.Clinical observation on treatment of heart failure with mixed diastolic and systolic dysfunction by shenqi fuzheng injection.
Xi-ping LIU ; Xing HAO ; Guan-xue XU
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(8):739-740
Adult
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Aged
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Cardiovascular Agents
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therapeutic use
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Diastole
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drug effects
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Drug Therapy, Combination
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Drugs, Chinese Herbal
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therapeutic use
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Female
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Heart Failure
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drug therapy
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physiopathology
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Humans
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Injections
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Male
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Middle Aged
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Phytotherapy
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Systole
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drug effects
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Ventricular Dysfunction, Left
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complications
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drug therapy
7.Application of rapid immunohistochemical staining technique in intraoperative frozen section diagnosis of thyroid neoplasm
Xi-lin LIU ; Ping-ling YANG ; Yong-sheng ZHANG ; Jie HAN ; Xiao-ling ZHU ; Ping GAO
Cancer Research and Clinic 2011;23(12):810-813
ObjectiveTo investigate the application of rapid immunohistochemical staining technique in intraoperative frozen section diagnosis of thyroid neoplasm.Methods MaxVision one-step rapid immunohistochemical staining technique was used to detect the expression of CK19,HBME-1,and Gal-3 in frozen section of papillary thyroid carcinoma(PTC)andthyroid benign lesions.MaxVision conventional immunohistochemistry of frozen remaining tissue was served as control.ResultsMaxVision one-step rapid immunohistochemical staining technique could be completed in 20 minutes.The positive localizations of three markers detected by rapid immunohistochemistry were similar to conventional immunohistochemistry, in general.The expression of CK19 was located in cytoplasm and cellular membrane.Gal-3 and HBME-1 were mainly detected in follicular luminal border and/or surface of papilla. The staining intensity in rapid immunohistochemistry was stronger than that in conventional immunohistochemistry. The positive rates of CK19,HBME-1,and Gal-3 by rapid immunohistochemistry in frozen sections were: 0 (0/28),10.7 % (3/28),0 (0/28),respectively,for benign lesions (nodular goiter,Hashimoto thyroiditis,thyroid adenoma); and 94.9 %(37/39),92.3 % (36/39),92.3 % (36/39),respectively,for PTC.The expression of three markers between thyroid benign lesions and PTC had a significant difference (x2 =59.326,55.861,44.605,all P < 0.001).In benign lesions,the rate of same case with two and more positive markers was 0,while in PTC it was 100 % and significantly different (x2 =67.000,P < 0.05).ConclusionMaxVision one-step rapid immunohistochemical staining technique could be applied in intraoperative frozen section diagnosis.Detecting CK19,HBME-1,and Gal-3 expression in intraoperative frozen section has an auxiliary value for diagnosis of PTC.
8.Synergetic use of tripterygium wilfordii and aescine for treatment of posttraumatic acute lung injury
Chaopu LIU ; Jinmou GAO ; Ping HU ; Changhua LI ; Ping HE ; Gongbin WEI ; Xi LIN
Chinese Journal of Trauma 2013;29(12):1180-1183
Objective To investigate the effect and mechanism of tripterygium wilfordii combined with aescine in treatment of acute lung injury (ALI) following trauma.Methods A total of 120 patients with posttraumatic ALI were divided into four groups:tripterygium wilfordii + aescine group (combined group),tripterygium wilfordii group,aescine group,and control group according to random number table,with 30 patients per group.Regardless of the same conventional therapy,the patients in combined group were treated by orally or gastrically administered tripterygium wilfordii multiglycoside as well as aescine intravenously,once a day for 12 days; on the contrary,the same method but only with tripterygium wilfordii or aescine was respectively performed in tripterygium wilfordii and aescine groups.Blood gas analysis before and after treatment,length of ICU stay,fatality rate and incidence of ARDS in each group were observed.Changes in levels of inflammatory mediators (TNF-oα,IL-1,IL-6 IL-8,etc)were detected at days 4,8,and 12 after medication.Results Shorter length of ICU stay and lower ARDS incidence as well as 12-day fatality rate were detected in each treatment group as compared with control group,especially in combined group (P < 0.05).Respiratory rate,PaO2 and oxygenation index (PaO2/FiO2) in each treatment group presented better amelioration than those in control group and especially better results were observed in combined group (P < 0.05).Plasma levels of TNF-α,IL-1,IL-6,and IL-8 in each treatment group were lower than those in control group (P < 0.05).Conclusions Tripterygium wilfordii and aescine havetherapeutic effect on posttraumatic ALI.Moreover,synergetic use of the two drugs achieves synergistic effect and better outcome.
9.Arthroscopically minimal invasion asisted percutaneous cannulated screw fixation of longitudinal fracture of the patella
Xi-Zeng NIE ; Xiang-Yi MA ; Shu-Min LIU ; Feng LI ; Jin-Hui LIU ; Ping LIU ;
Chinese Journal of Primary Medicine and Pharmacy 2005;0(11):-
Objective To evaluate the effectiveness of arthroscopically assisted percutaneous reduction and internal fixation with eannulated screws.Methods The fracture was reduced by closed manipulation or percutaneous leverage force by using the Kirschner wire.Then the patella was temporarily fixed by using a large size towel clamp or Kirschner wires.Under the guidance of knee arthroscopy,a micro-incision was made at the size of cannulated screw placement,the pilot holes were drilled at a proper depth,and the thread was configured.Two titanium screws were inserted parallelly.Results Following-up chekups for 4~24 months in 18 cases showed a satisfactory recovery of knee functions.According to the Bostman' standard,excellent effects were obtained in 16 cases and good effects in 2 cases.Conclusion Treatment of patellar fractures by percutaneous cannulated screw fixation under arrhroscope of- fered advantages of minimal invasion,accurate reduction,reliable fixation,and excellent recovery of joint functions.
10.Analysis of active surveillance on the etiology of foodborne diseases in Xi'an in 2017-2019
Ping LIU ; Feng ZHANG ; Dong LIU
Journal of Public Health and Preventive Medicine 2021;32(6):36-39
Objective To analyze the epidemiological characteristics and the detection of pathogenic microorganisms in the cases of foodborne diseases under active surveillance in Xi'an, and to provide references for prevention and control of foodborne diseases. Methods Information on surveillance cases of foodborne diseases reported by two sentinel hospitals in Xi'an from 2017 to 2019 was collected. Data was statistically analyzed. Results From 2017 to 2019, a total of 904 cases of active surveillance of foodborne diseases were reported by two active surveillance sentinel hospitals in Xi'an. There were more cases in the age groups of 1-5 and 5-8, accounting for 35.18% and 20.02%, respectively. There was a statistically significant difference in the number of cases reported in four quarters(χ2=545.38,P<0.05). The majority of cases were reported in the third quarter, accounting for 53.76% of the total. The majority of suspicious foods were mainly mixed foods and the most suspicious dining places were families. Of the 901 samples, 251 were positive with a positive rate of 27.86%. There was a statistically significant difference in the detection ratio of different foodborne pathogens(χ2=321.92,P<0.05). The detection rates of Norovirus and salmonella were higher, accounting for 14.98% and 11.43%, respectively. There was a statistically significant difference in the detection rate of Salmonella in different seasons(χ2= 26.49,P<0.05), and the detection rates were higher in the second and third quarter. There was also a statistically significant difference in the detection rate of Norovirus(χ2=92.17,P<0.05) in different seasons, and the detection rate was higher in the first and fourth quarters. Conclusion The main pathogens of food-borne diseases are Norovirus and Salmonella in Xi'an. It is suggested that active surveillance should be strengthened. The prevention and control knowledge of foodborne diseases should be carried out according to the epidemiological characteristics.