Breast Neoplasms ; pathology ; surgery ; Carcinoma, Ductal, Breast ; pathology ; surgery ; Cryoultramicrotomy ; Cytodiagnosis ; methods ; Female ; Histocytological Preparation Techniques ; methods ; Humans ; Intraoperative Care ; methods ; Precancerous Conditions ; pathology ; surgery

Objective To construct a system for three dimensional face scanning and measurement. Methods The measurement system was based on the principle of triangulation and the combination of gray-code and phase-shift structured light projection. The system software was developed for Windows XP with the aid of tools such as Visual C++ and Hoops. Results A three dimensional measurement system based on structured light projection was developed. The system hardware was composed of fringe projection unit, image gathering unit, system control unit and mechanical appearance, and the system software was composed of point cloud display and editing module. The lamp house of the system was 12V, the working distance was 900 mm, the scanning time was 5.5 s and the scanning field was 500 mm×400 mm. Conclusion The three dimensional measurement system based on structured light projection is a refined machine with safe light to eyes, and the accuracy and scanning speed are suitable to face scanning.

Hernia, Inguinal ; diagnostic imaging ; Humans ; Male ; Middle Aged ; Peritoneal Dialysis, Continuous Ambulatory ; Peritoneum ; diagnostic imaging ; Tomography, X-Ray Computed ; methods

OBJECTIVETo evaluate the application of sentinel lymph node (SLN) biopsy in diagnosis and treatment of gastric cancer.

METHODSThirty- eight patients with gastric cancer were divided into three groups (T(1); T(2); T(3)) according to tumor invasion depth. SLN was localized using methyl blue injection method. Metastatic lymph nodes were detected by immunohistochemical staining of cytokeratin (CK- 19).

RESULTSSLN(S ) were detected in all 38 patients and lymph node metastasis occurred in 18 cases. The sensitivity, false- negative rate, and diagnostic accuracy of SLN for lymph node metastasis were 83.3% 16.7% and 92.1% respectively. The diagnostic accuracy was 100% in T1 cases without false- negative cases, 94.1% in T(2) cases with one false- negative case, 75% in T(3) cases with two false- negative cases.

CONCLUSIONThe method of injecting methylene blue around the primary tumor is a feasible method that can determine SLN localization during operation.

Adult ; Aged ; Female ; Humans ; Male ; Methylene Blue ; Middle Aged ; Neoplasm Staging ; Sentinel Lymph Node Biopsy ; Stomach Neoplasms ; diagnosis ; pathology

Objective To discuss the temperature difference of infant incubator treatment for the baby to provide important guarantee, to ensure its safe operationMethods Using a completely independent of the infant incubator's temperature monitoring and alarm system of infant incubator temperature and monitoring and alarm system, 20 sets of real-time monitoring and automatic logging data, different brand infant incubator temperature changes compared with infant incubator's own body temperature, and analysis.Results20 sets of different brand infant incubator, 10(50%) in the devices panel display data with a monitoring device differences in measured data, clinical pose a safety hazard. Conclusions For clinical use of infant incubator temperature real-time monitor, by the monitoring system for additional auxiliary monitoring and alarm, thereby improve infant incubator clinical application security, reduce the incidence of related medical accidents, improve the quality of medical treatment.

Township and Village Health Services Integration Management (TVHSIM) is an essential form of China's two-tiered health service integration plan at the township and village level.Its main purpose,also one of the target goals in China's new healthcare reform,is to gradually integrate rural health services and appropriately allocate rural health resources.This study aims to assess the village doctors' satisfaction with the TVHSIM and provide scientific base to further improve TVHSIM.A cross-sectional study was carried out in which 162 village doctors from Qinghai,Inner Mongolia and Xinjiang in western China were interviewed.Descriptive analysis,independent t-test,one-way ANOVA,Spearman rank correlation and multiple linear regression were used to analyze the difference and relevance between village doctors' personal characteristics and their satisfaction with TVHSIM and six subscales.Village doctors with different years of practice,social insurance status and essential medical knowledge level showed statistically significant differences in their satisfaction levels (all P＜0.05).Age (P＜0.05) and years of practice (P＜0.01) were negatively correlated with Drug and Medical Device Management and Financing Management.Essential medical knowledge level (P＜0.05) was negatively correlated with Operations Management as well.However,social insurance status (P＜0.05) was positively correlated with Human Resources Management and Drug and Medical Device management.Gender,age and years of practice respectively had significant influence on village doctors' satisfaction with TVHSIM (P＜0.01).In conclusion,in order to further promote TVHSIM policy in rural China,a well-rounded social insurance model for village doctors is urgently needed.In addition,the development of TVHSIM is regionally imbalanced.Efficient and effective measures aiming at rationalizing gender and age structure and enhancing essential medical training should be carefully considered.

Objective:To study confirm whether curcumin could inhibit the proliferation of CD44+colon cancer cells. In this study. Methods: MTT method was used to test the proliferation inhibition effects of curcumin against colon cancer cells. And Real-time PCR and Fluorescence-activated cell sorting (FACS) were used to determine CD44 expression level in colon cancer cells after treated with curcumin. Then we used Magnetic-activated cell sorting ( MACS) method to separate CD44 positive and negative cells. And we compared the proliferation inhibition effects of curcumin against these two kinds of cells. Results: Curcumin could inhibit the proliferation of HCT116 and HT-29 colon cancer cell lines. The expression level of CD44 could also be down-regulated after the colon cancer cells were treated with curcumin. Conclusion: Curcumin could inhibit the proliferation of CD44+colon cancer cells. Thus it was suggested that crcumin might have the effect to inhibit the proliferation of colon cancer stem cells and might be used as a promising agents to prevent the relapse and metastasis of colon cancer.

OBJECTIVETo analyze the clinical character, diagnosis and treatment of badly necrotic pyogenic infection in cervical part with pneumatosis of vomica.

METHODSThe clinical character, diagnosis and treatment of badly necrotic pyogenic infection in cervical part with pneumatosis of vomica of 46 cases were investigated retrospectively.

RESULTSLateral and bilateral neck infection cases were 38 and 8 respectively. 30 cases formed primary pyogenic infection in cervical part with pneumatosis of vomica, and 16 did from adjacent sites. Besides the characters of the acute infection, gas storage in deep cervical part abscess was notable. CT and B Ultrasonic examination provided useful informations such as sizes, shapes, capacity, extents of abscess and the relationship between the abscess and vessel or vital organ. Diagnosis puncture and germiculture were performed before and after operation. The results showed that 25 of 46 cases were infected by staphylococcus or streptococcus, and 21 cases did by other bacterium. Exploration and drainage treatments were performed. All cases were cured except 2 died.

CONCLUSIONDiagnostic puncture, CT and/or B Ultrasonic examination are essential for diagnosis and presurgical planning. Germiculture provides reliable evidence for finding pathogeny and therapy. The most possibilities of pyogenic infection with pneumatosis of vomica in cervical part are the action of aerogenic bacterium, infection both in cervical part and chest or swallowing movement of pharynx.

Abscess ; diagnosis ; microbiology ; therapy ; Cysts ; diagnosis ; microbiology ; therapy ; Humans ; Neck ; pathology ; Necrosis ; diagnosis ; microbiology ; therapy

OBJECTIVETo investigate the effect of tricostantin A (TSA) on self-renewal of breast cancer stem cells and explore the mechanisms.

METHODSBreast cancer cell lines MDA-MB-468, MDA-MB-231, MCF-7 and SKBR3 were cultured in suspension and treated with different concentrations of TSA for 7 days, using 0.1% DMSO as the control. Secondary mammosphere formation efficiency and percentage of CD44(+)/CD24(-) sub-population in the primary mammospheres were used to evaluate the effects of TSA on self-renewal of breast cancer stem cells. The breast cancer stem cell surface marker CD44(+)/CD24(-) and the percentage of apoptosis in the primary mammospheres were assayed using flow cytometry. The mRNA expressions of Nanog, Sox2 and Oct4 in the primary mammospheres were assayed with quantitative PCR.

RESULTSTSA at both 100 and 500 nmol/L, but not at 10 nmol/L, partially inhibited the self-renewal of breast cancer stem cells from the 4 cell lines. TSA at 500 nmol/L induced cell apoptosis in the primary mammospheres. TSA down-regulated the mRNA expression of Nanog and Sox2 in the primary mammospheres.

CONCLUSIONTSA can partially inhibit the self-renewal of breast cancer stem cells through a mechanism involving the down-regulation of Nanog and Sox2 expression, indicating the value of combined treatments with low-dose TSA and other anticancer drugs to achieve maximum inhibition of breast cancer stem cell self-renewal. The core transcriptional factor of embryonic stem cells Nanog and Sox2 can be potential targets of anticancer therapy.

Antineoplastic Agents ; administration & dosage ; pharmacology ; Apoptosis ; drug effects ; Breast Neoplasms ; metabolism ; pathology ; CD24 Antigen ; metabolism ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Dose-Response Relationship, Drug ; Down-Regulation ; Female ; Histone Deacetylase Inhibitors ; administration & dosage ; pharmacology ; Homeodomain Proteins ; genetics ; metabolism ; Humans ; Hyaluronan Receptors ; metabolism ; Hydroxamic Acids ; administration & dosage ; pharmacology ; Nanog Homeobox Protein ; Neoplastic Stem Cells ; metabolism ; pathology ; RNA, Messenger ; metabolism ; SOXB1 Transcription Factors ; genetics ; metabolism

The atom absorption spectrometer is introduced to determine the cation concentrations such as potassium, sodium, calcium and magnesium in the dialysis fluid. They are accurately determined by proper preparation and correct dilution ratio under the optimized measuring conditions. Compared with the current arbitrate methods (EDTA titration determination of calcium and magnesium), it supplements the methods of international and industrial standards for determination of cations.
Calcium ; analysis ; Cations ; analysis ; Dialysis Solutions ; analysis ; chemistry ; Magnesium ; analysis ; Potassium ; analysis ; Sodium ; analysis ; Spectrophotometry, Atomic ; methods