Objective To investigate the status of clinical using of blood products in Shanghai area,clinicians' understanding on transfusion knowledge and consciousness of prevention of transfusion risk.Methods Two hundreds of clinicians who were randomly selected from level two and above hospitals of Shanghai were inquired by questionnaire.Data including understanding of transfusion techniques,prevention of transfusion risk,application of blood components and grasp of new techniques were collected and analyzed.Results Among all interviewed clinicians,those who realized the importance of transfusion on clinical work,who understood the basic contents of clinical guideline for transfusion issued by Ministry of Health People's Republic of China,and who had the basic knowledge of blood component products and transfusion applications account for 62%,60% and 81% respectively.But only 28% clinicians knew all the side effects,27.3% learned the new techniques of transfusion,including leukocyte reduced,virus elimination,clinical application of irradiation.This investigation also showed 97% clinicians would like to accept training of transfusion,68% thought they need more relevant information about prevention of transfusion risk.Conclusions Clinicians in Shanghai area had the basic knowledge of clinical guideline for transfusion,but some knowledge of transfusion were insufficient including blood components and their applications,side effects and new techniques of transfusion.Clinicians in Shanghai had active response for being trained on knowledge of transfusion.

Based on a study of children’s behavior and psychological characteristics,the article introduced Maslow’s hierarchy of needs to discuss children’s special needs for medical environment.Hence the authors proposed to meet children’s physiological needs,security needs,social needs,esteem needs,and self-realization needs.Shanghai Children’s Hospital was cited as an example,to present the humanistic expression practices in designing children’s medical environment.

Objective To observe the survival of EAE rats after recombinant adenovirus NgR specific RNA interference(Ad-shRNA-NgR) transfected the brain tissue of the experimental autoimmune encephalomyelitis(EAE),and provide the basis for EAE intervention.Methods EAE rats were randomly divided into high,medium,low and control groups(20 rats in each group).The lateral ventricle of EAE rats were injected with a titer of 1×1011 pfu/mL,1×1010 pfu/mL and 1×109 pfu/mL Ad-shRNA-NgR.The survival of EAE rats at third and seventh days after injection was observed.Results The survival rate of EAE rats of the high titer group was significantly lower than those of the middle titer group and low titer group at third and seventh days after Ad-shRNA-NgR transfected into EAE brain tissue.There was no significant difference in survival rate in middle titer group,low titer group and control group.Conclusion The titer of Ad-shRNA-NgR is safe in the experiment of EAE rats from 1×1010 pfu/mL to the range of 1×109 pfu/mL.

Objective: To establish the quality standard for Tiaolitongbao I capsules.Methods: The components including Astragalus , Atractylodes, Finger citron and Radix aucklandiae were identified by TLC.Emodin, the effective component of Polygonum cuspidatum , was determined by HPLC.Results: The characteristic spots in TLC were clear without any interference.The linear range of emodin was 4.25-68.00 μg·ml-1 (r =0.999 9).The average recovery was 95.22% ,and RSD was 1.47% (n =6).Conclusion: The methods used for the identification and quantification are sensitive, simple and accurate, which can be used for the quality control of Tiaolitongbao I capsules.

Adult ; Female ; Humans ; Myocardial Infarction ; complications ; Pregnancy ; Pregnancy Complications, Cardiovascular

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Female ; Heavy Metal Poisoning ; Humans ; Male ; Middle Aged ; Poisoning ; diagnosis ; therapy ; Prognosis ; Thallium ; poisoning ; Treatment Outcome ; Young Adult

Objective To estimate the 10-year risk of coronary heart disease in patients with psoriasis,and to determine whether the risk profile is affected by disease duration,family history and clinical types of psoriasis.Methods Data were pooled from 322 inpatients with psoriasis and 297 inpatients with other skin diseases (151 cases of acute urticaria,33 drug eruption,113 herpes zoster) from December 2008 to December 2010 in the Department of Dermatology,West China Hospital of Sichuan University.Risks of coronary heart disease were estimated by using the Framingham risk score algorithm.Wilcoxon rank test,Kruskal-Wallis test and chi-square tests were used to evaluate the differences in risks and risk factors between the patients with psoriasis and controls.Results As far as the Framingham 10-year risk score was concerned,the difference was statistically significant between the patients with psoriasis and the control population (6％ vs.4％,Z =4.342,P =0.000),as well as between patients with different clinical types of psoriasis (6％ for psoriasis vulgaris vs.4％ for pustular psoriasis vs.7％ for psoriasis arthropathica vs.7％ for erythroderma psoriaticum,H =8.484,P ＜ 0.05),but was insignificant between patients with familial and sporadic psoriasis (6％ vs.6％,Z =0.406,P ＞ 0.05 ).No correlation was observed between the Framingham 10-year risk and psoriasis duration (P ＞ 0.05 ).Conclusions Increased risks of coronary heart disease exist in patients with psoriasis compared with those with acute urticaria,drug eruption or hepers zoster,and in patients with psoriasis arthropathica or erythroderma psoriaticum compared with those with pustular psoriasis.The Framingham 10-year risk of coronary heart disease is uncorrelated with disease duration and unaffected by family history of psoriasis.

In order to understand the alcohol's toxicity to the quantitative alternations of synapses in mouse visual cortex, the expression of synaptophysin after prenatal alcohol exposure was investigated. In present study, the experimental mice at P0, P7, P14 and P30 were grouped, as control, 2 g x kg(-1) alcohol treatment and 4 g x kg(-1) alcohol treatment. The pre-synaptic elements which were used to represent synapses were marked with synaptophysin (a synaptic vesicle associated protein) by immunocytochemistry technique. The synaptophysin positive boutons in layer VI of visual cortex were imaged under laser confocal microscope. With stereological methods, the number cal density of synapse in visual cortex was calculated in different groups at various ages. Moreover, Western blotting was carried out to detect the expression of synaptophysin in visual cortex. The results showed that prenatal alcohol exposure could cause synaptic loss with long-term effect and in a dose dependent manner. For instance, there were significant difference among the different treatment groups of P0, P14 and P30 as well (P < 0.05). Western blotting supported the results of immunofluorescent labeling. In conclusion, prenatal alcohol exposure can induce the synaptic loss dose dependently and with long-term effect. Our findings implicate that the synaptic loss with long-term effect in CNS probably contributes to the lifelong mental retardation and memorial lowliness associated with childhood FAS.

Objective To evaluate the clinical application of susceptibility-weighted imaging(SWI) in the early diagnosis of massive cerebral infarction with hemorrhage and analysis of collateral circulation.Methods Fifty patients with massive cerebral infarction underwent MRI (T1WI,T2WI,DWI,MRA and SWI) scan in acute stage and decubation respectively in Hanrison International Peace Hospital from January 2012 to January 2014.Analysis T1WI,T2WI,DWI,MRA and SWI differences in the delection of cerebral infarction with hemorrhage and analysis of Collateral circulation.Record the magnetic resonance angiography hemorrhagic transformation and display the ischemic area of collateral circulation in the detection of cerebral infarction remodeling differences.Results (1)SWI detected out 18 cases of cerebral infarction with hemorrhage (36％),including 14 cases of hemorrhagic infarction(HI) type(28％),4 cases of parenchymal hemorrhage(PH) type 8％).MRI detected 11 cases of cerebral infarction with hemorrhage (22％),including 7 cases of HI type 14％),4 cases of PH type (8％).SWI detected 106 focuses,the detection rate was 100％,MRI detected only 26,and the detection rate was 32.51％,and the missing mainly for micro hemorrhage.There was statistical significance between the groups(x2 =21.045,P＜0.05).(2) SWI can display the condition of micrangium in the area of cerebral infarction clearly.The number of micrangium decreased or disappeared in acute stage,but the micrangium increased in decubation.The ratio of capillary vessels increased to 66％.MRA detected 82％ of criminal vessel in patients with cerebral infarction.Conclusion SWI is more sensitive than conventional MR in the early diagnosis of massive cerebral infarction with hemorrhage.MRA combined with SWI could not only detect the criminal vessel of cerebral infarction area,but also monitor the reconstruction of collateral circulation in the infarction region,and there will be extremely valuable to the treatment massive cerebral infarction and evaluation of prognosis.

Objective To investigate the effects of c-Jun N-terminal kinase (JNK) 2 on NKG2D-mediated natural killer ( NK) cell cytotoxicity .Me thods NK cells were activated by polyinosinic-polycyti-dylic acid ( Poly I∶C ) .The activation status of JNK signaling pathway was detected .The cytotoxicity of ac-tivated NK cells and the level of IFN-γproduced were measured to determine the function of NK cells in the absence of JNK2.Tumor growth in wild type and JNK2-knockout (JNK2-/-) mice with lymphoma xenograft were measured to evaluate immune surveillance of NK cells .Results The phosphorylation of JNK and up-stream kinases were observed in the early stage of cell activation after treatment of Poly I ∶C.The expressed ligands of the activating receptors NKG2 D significantly increased NK cell cytotoxicity to lymphoma cells . JNK2 deficiency impaired the antitumor effects of NK cells , and then resulted in enhanced tumor growth in JNK2-/-mice.Conclusion JNK2 signaling pathway is involved in the NKG2 D-mediated activation of NK cells and regulates immune surveillance of NK cells against tumor .