Objective To study the application of contact oxidization and hydrolysis acidifying technology in hospital wastewater treatment. Methods Hydrolysis combined with chlorine dioxide disinfection was used in the engineering of treatment. Results Even if CODCr was 300 mg/L, BOD5 was 150 mg/L, SS was 300 mg/L, pH was 6-9 and large intestine bacteria number was 50 000/L, the wastewater quality met the requirement of related standard after treated with the present method. Conclusion The present technology was efficient, practical and economical.

Objective To investigate the bacterial distribution and resistance of clinical isolates ,so as to provide basis for the clinical medication .Methods The drug susceptibility testing was performed by Kirby‐Bauer method or automated system .The re‐sults were analyzed by WHONET 5 .6 referring to the breakpoints of CLSI 2013 .Results Of 1 240 clinical iso1ates ,the gram‐posi‐tive cocci and gram‐negative bacteria accounted for 32 .7% and 67 .3% respectively .The top five of isolated strains were Escherichia coli ,Klebsiella spp ,coagulase negative staphylococci ,Staphylococcus aureus ,Streptococcus pneumonia;the first five of isolated spec‐imens constitute followed by sputum ,urine ,blood ,secretions and stool .The average prevalence of methicillin‐resistant strains in S . aureus (MRSA) and coagulase‐negative Staphylococcus (MRCNS)was 17 .1% and 74 .7% ,respectively ;no vancomycin ,teicoplanin or linezolid‐resistant strains were found;no vancomycin‐resistant strains were found in Enterococcus spp;Enterobacteriaceae was still highly susceptible to imipenem and meropenem (> 85% );extended spectrum beta‐lactamases(ESBLs) producing strains ac‐counted for 56 .7% of Escherichia coli ,and accounted for 18 .6% of Klebsiella pneumonia;the drug resistance rates of Pseudomonas aeruginosa to imipenem was 11 .9% .The drug resistance rates of Acinetobacter spp to imipenem was 19 .0% .Conclusion The main infection site of patients in this hospital is respiratory tract and attention should be paid to docimastic sample .The top five clinical i‐solates and drug resistance are roughly the same with the results of Chongqing drug resistance monitoring net .

Objective:To analyze data of 5604 cases who received health examination in our hospital ,and understand morbidities of overweight and obesity in these people and their relationship with blood glucose level . Methods :Morning venous blood was taken from the 5604 subjects undergoing health examination to measure blood glucose lev‐el ,and their body weight and height were measured at the same time ,then body mass index (BMI) was calculated and analyzed .Results:Among the 5604 subjects , prevalence rates of overweight and obesity were 42.1% and 13. 3% respectively ,and they were 50% and 16. 4% in men ,and 25. 4% and 6. 8% in women respectively .Among different age groups (except ≥71 years group) , prevalence rate of overweight in men was significantly higher than that of women , P<0.01 all;except ≥61 years subjects , prevalence rate of obesity in men was significantly higher than that of women ,P<0.01 .In overweight group ,risk relative (RR) of impaired fasting blood glucose and dia‐betes mellitus to those of subjects with normal body weight was 2.32 (95% CI 1.91~2.82) and 2.92 (95% CI 2.31~3.70) respectively ;in obesity group , RR of impaired fasting blood glucose and diabetes mellitus to those of subjects with normal body weight was 3.2 (95% CI 2.57~3.99) and 5.17 (95% CI 4.53~5.90) respectively , P=0.001 all . Conclusion:Overweight and obesity have become a common problem among our customer groups .Compared with those with normal body weight ,overweight and obesity greatly increase risk ratio of impaired fasting blood glucose .

Objective To compare the clinical outcomes between oblique and non-oblique fixation with cannulated screws for femoral neck fractures in the elderly patients.Methods Totally 189 patients of over 65 years old were treated for femoral neck fracture from June 2007 to October 2013 at our department.Oblique fixation with cannulated screws was used in 130 cases.They were 43 males aud 87 females with an average age of 77.8 ± 7.5 years.According to Garden classification,40 cases were of Garden types Ⅰ and Ⅱ (undisplaced fractures),and 90 of Garden types Ⅲ and Ⅳ (displaced fractures).Of them 26 were Pauwells type Ⅲ.Non-oblique fixation with cannulated screws was conducted in 59 cases.They were 24 males and 35 females with an average age of 75.5 ± 8.2 years.According to Garden classification,12 cases were of Garden types Ⅰ and Ⅱ (undisplaced fractures),and 47 of Garden types Ⅲ and Ⅳ (displaced fractures).Of them,15 were Pauwells type Ⅲ.The fracture union,postoperative complications,rate of reoperation and Harris scores at the last follow-up were compared between the 2 groups.Results All the 189 patients were followed up for 10 to 38 months (average,17.4 months).The oblique fixation led to a union rate of 99.2％ (129/130),a fixation failure rate of 3.1％ (4/130) and a femoral head necrosis rate of 2.3％ (3/130),which were not significantly different from those resulted from the non-oblique fixation [98.3％ (58/59),10.2％ (6/59) and 5.1％ (3/59) respectively] (P ＞ 0.05).However,the rate of femoral neck shortening [35.4％ (46/130)] and the reoperation rate [3.1％ (4/130)] in the oblique fixation group were significantly lower than those in the non-oblique fixation group [50.8％ (30/59) and 15.3％ (9/59) respectively] (P ＜ 0.05).The mean Harris hip score at the last follow-up for the oblique fixation(75.7 ± 14.3) was significantly higher than that for the non-oblique fixation (70.8 ± 16.7) (P ＜ 0.05).Conclusion As oblique cannulated screwing can reduce the rates of femoral neck shortening and reoperation to some extent,it may be a better fixation choice than the non-oblique cannulated screwing for femoral neck fracture in elderly patients.

The treatment of multiple myeloma has become possible in the era of novel agents. Novel agents have dramatically improved the response rates of multiple myeloma (MM) and have extended the survival of patients even if MM remains an incurable disease. De-termining the treatment goals of MM has become a major challenge for clinicians in the era of novel agents. Many results show the correlation between response depth and patients' survival. Complete remission (CR), stringent CR, phenotypic CR, and CRs at the molecular and PET-CT levels are being explored and becoming the goals of MM treatment. Doctors should balance efficacy with adverse effects in daily clinical practice to avoid the blind pursuit of a high and deep response rate.

BACKGROUND:Three-dimensional (3D) nanofiber scaffolds are aimed to mimic the physical and chemical signals of stem celsin vivo. 3D nanofiber scaffolds, which are capable to maintain the activity of stem cels, are promising in tissue regeneration and stem celltherapy. OBJECTIVE:To review the synthesis methods for 3D nanofiber scaffolds, and the interactions of stem cels and 3D scaffolds as wel as the current progress of 3D nanofiber scaffolds in tissue engineering. METHODS:Web of Science was searched with key words of “tissue engineering, nanofiber scaffold, stem cellfate” in English for articles relevant to stem celltissue engineering and 3D nanofiber scaffolds. RESULTS AND CONCLUSION:3D nanofiber scaffolds can mimic the physical architecture ofin vivo microenvironment due to its nano-scale topology. Chemical modification of scaffolds endows chemical cues to stem cels. Therefore, 3D nanofiber scaffold can be a promising delivery vehicle of stem cels in tissue engineering. Nanofiber scaffolds can be synthesized through self-assembly, sol-gel phase separation, and electric spinning. 3D nanofiber scaffolds have been shown to improve thein vitro proliferation of hematopoietic stem cels, embryonic stem cels, mesenchymal stem cels, and neural stem cels. 3D nanofiber scaffolds with specific topology/chemical properties can induce the differentiation of stem cels into bone, cartilage, nerve, or muscle. 3D nanofiber scaffold which provides a satisfactory microenvironment for stem cellenhance the performance of stem celltherapy.

BACKGROUND:Self-assembling peptide scaffolds have attracted much more attention among three dimensional biomaterials due to its biocompatibility, biodegradation, and tailor-made properties.
OBJECTIVE:To review the structure and design of functionalized self-assembling RADA peptide, and the recent advances in the use of RADA self-assembling peptide for three-dimensional cel culture in cel therapy applications.
METHODS:Total 224 literatures related to self-assembling peptide and tissue engineering from PubMed, Web of Science and CNKI databases were screened out for this review. The keywords were“self-assembly peptide, tissue engineering”in English and Chinese, respectively. Final y 48 of 224 articles about the design, fictionalization, and three-dimensional cel culture of peptide scaffolds were included in this review.
RESULTS AND CONCLUSION:Self-assembling peptide could undergo spontaneous assembly into wel-ordered interwoven nanofibers in water and rapidly form hydrogel, which physical y mimics the architecture of natural extracel ular matrix to ensure a real three-dimensional microenvironment for cel s. In terms of bio-function, this material can be tailor-made with various bioactive short peptide motifs to promote cel adhesion, proliferation, and differentiation.

Bronchoscopes ; Bronchoscopy ; instrumentation ; methods ; Child ; China ; Humans

gress. A growing body of research has committed to exploring more thrombolytic regimens. This article reviews the main methods, drugs and recent progress in thrombolytic therapy.