AIM:To prepare O/W microemulsion formulation containing sinomenine and investigate the effect of different penetration enhancers on transermal delivery character of microemulsion in vitro. METHODS: The microemulsions were prepared and characterized using Zetasize potential/particle size analysis instrument.The stability tests were performed to evaluate the sinomenine microemulsions.Franz diffusion cell was adopted as the apparatus for in vitro transdermal test.Excised Wistar-rat abdomen skin was pretreated with penetration enhancers,such as menthol,borneol and isopropyl myristate. RESULTS: The average droplet size of the microemulsion was about(31.3 nm.)A high permeation rate of(32.238?1.13)?g/cm~(2)?h was showed in vitro.Ail the enhancers showed significant enhancement effect on the transdermal behavior of sinomenine microemulsion.2% borneol had the strongest effect in all. CONCLUSION: These results indicate that the studied microemulsion system with high permeation rate may be a promising vehicle for the transdermal delivery of sinomenine.Menthol,borneol and isopropyl myristate were found to be the proper penetration enhancers in the transdermal preparation.

Systemic lupus erythematosus (SLE) is a kind of complex autoimmune connective tissue disease involving multi-organ and multiple systems.And the cardiovascular complication is a major cause of mortality in SLE patients.The diagnosis and evaluation depend on imaging.As a non-invasive,simplicity and repeatability method,echocardiography plays a vital role in evaluating heart structure and function.The application of echocardiography in the SLE with cardiovascular complications were reviewed in this article.

Professor LI Guo-heng is one of famous TCM old doctors in our country.He is the main outstanding inheritor of Wei Shi Traumatology.He is skill in internal and external use of Chinese herb,manipulation and body guidance technique.He is good at treating injury congestion syndrome,Jin Chu Cao,Gu Cuo Feng with Wei Shi Traumatology.Combining differentiation syndrome with differentiation diseases,and using Chinese herb and manipulation,which has a good clinical efficacy.

AIM:To set up the quality standard for Fuyang Jiedu Granules(Radix Sutellariae,Herba Scute-(llariae) Barbatae,Herba Epimedii,etc.). METHODS: Radix Scutellariae,Herba Scutellariae Barbatae and Herba Epimedii in preparation were identified by TLC.The content of baicalin was determined by HPLC on Agilent Eclipse XBD-C_(18) column. The mobile phase was methanol-phosphoric-water(47∶53∶0.2).The detection wavelength was at 280 nm. A Shim-pack VP-ODS column was used to determine icariin.The mobile phase consisted of 0.32% phosphoric acid-acetonitrile(75∶25).The detection wavelength was 270 nm. RESULTS: The spot on the thin layer plate was clear.The negative contral was not disturbed.The linear range of baicalin was in the range 0.122 12 ?g to 0.606 ?g.The average recovery was 99.59% with RSD of 2.16%.The linearity range of icariin was in the range 0.201 6 ?g-1.008 ?g.The average recovery was 99.49% with RSD of 1.31%.(CONCLUSION:) The methods are specific,simple,fast and accurate,and can be used for quality control of Fuyang Jiedu Granules.

Objective The regulation on glucose transporters(GLUT1~GLUT5)by sodium butyrate was observed,and the regulation on bax and bcl-x/l by sodium butyrate and glucose was studied.This helped to learn about the possible mechanism of apoptosis of cancer cells induced by sodium butyrate.Methods The expression of GLUT1~GLUT5 mRNA was detected by RT-PCR.The expression of bax and bcl-x/l was detected by immuocytochemistry.Cell apoptosis was detected by TUNEL assay.Results In HT-29 cell line,sodium butyrate downregulated the expression of GLUT1 mRNA and the expression of bcl-x/l and induced cancer cell apoptosis,but did not regulate the expression of GLUT2,GLUT3,GLUT5 and bax.GLUT4 was not detected in HT-29 cell line.And when treated with sodium butyrate,the increase of concentration of glucose could increase the expression of bcl-x/l and reduce the effect of apoptosis induced by sodium butyrate.Without treatment with sodium butyrate,the change of glucose concentration only had a feeble regulation on apoptosis.Conclusion Sodium butyrate can obviously downregulate the expression of GLUT1 and downregulation of the expression of GLUT1 is associated with the apoptosis induced by sodium butyrate in HT-29 cell line.

OBJECTIVE:To evaluate the cost-effectiveness of 3 therapeutic schemes in the treatment of Parkinson's disease (PD). METHODS:A total of 94 PD cases were randomly assigned into 3 groups:Group A (Benserazide tablets +carbidopa and Levodopa controlled release tablets),Group B (Benserazide tablets+Carbidopa and Levodopa controlled release tablets+Selegiline tablets) and Group C (Benserazide tablets+Carbidopa and Levodopa controlled release tablets+Entacapone tablets). After treatment for 8 weeks,the cost-effectiveness analysis were conducted for all the 3 groups. RESULTS:For Group A,B and C,the costs were 448.78,803.89,1 559.89 yuan,respectively; the total effective rates were 56.67%,83.33% and 85.71%,respectively; the cost-effectiveness ratios were 791.92,964.71,1 819.96,respectively. The incremental cost-effectiveness ratios of Group B and C were 1 332.00 and 3 826.14,respectively as against Group A. CONCLUSION:Group B is preferable for PD among the 3 groups.

Objective:To establish the model of pancreaticoduodenal transplantation in rats. Methods:The enteric drained whole pancreas duodenal transplantation model was investisated. The donor aortic segment including the celiac, superior mesenteric divergence were anastomosed with recipient abdominal aorta with end to side. The donor portal was cuff-anastomosed with left renal vein. The graft duodenum were anastomosed with the host jejunum with end to side. Results:Eighty-nine operations were performed and 51 rats were successful, of which all graft of successful case had endocrinium function. Conclusion:This model for pancreas transplantation is reliable.

This article studies the application value of disposable umbilical cord clamp and amputator during gravida childbirth.The umbilicalis is clamped and sheared with disposable umbilical cord clamp and amputator in the area of 3~5mm far from the neonatal abdomen.The operation is simple and consumedly shortens the time and reduces the procedure compared to the traditional method.It is safe and convenient,which is worth spreading.

Epigenetics is defined as heritable changes in genc expression that are,unlike mutations,not attributable to alterations in the sequence of DNA.The predominant epigenetic mechanisms are DNA methylation,histone modification and microRNA.Exploring the precise roles of epigenetic factors on autoimmunity has become an active research area.Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disorder with a prevalence of approximately 0.5％ to 1.0％ worldwide.The mechanisms underlying the initiation and progression of RA are still not completely understood.In recent years,the epigenetics of RA have been widely investigated.Alterations in the modification of histones and DNA methylation are the two major epigenetic mechanisms that may potentially cause a breakdown of immune tolerance and the perpetuation of RA.These epigenetic changes may be ideal targets for new personalized treatments.

ObjectiveTo investigate osteopontin (OPN) level in pulmonary tuberculosis and its clinical significance.MethodsThe level of serum OPN was determined in 80 pulmonary tuberculosis patients before anti-tuberculosis treatment[patients group,simple pulmonary tuberculosis with 60 cases (simple pulmonary tuberculosis group),pulmonary tuberculosis combined with tuberculous pleurisy with 20cases (pulmonary tuberculosis combined with tuberculous pleurisy group)],and compared with that of 30healthy persons (healthy control group) and 20 patients having received effective anti-tuberculosis treatment and followed-up for 6 months (followed-up group).At the same time,the clinical significance of OPN was studied by other commonly used clinical indicators of tuberculosis.ResultsThe level of serum OPN in patients group [(843.49 ±569.23) ng/L] was significantly higher than that in healthy control group [(352.50 ± 185.02) ng/L,P ＜0.05].The level of serum OPN after treatment in followed-up group were significantly decreased compared with those before treatment [(494.11 ±352.40) ng/L vs.(1106.60 ± 628.39) ng/L,P＜ 0.05 ] and there was no significant difference compared with those in healthy control group (P ＞ 0.05 ).The level of serum OPN in pulmonary tuberculosis combined with tuberculous pleurisy group [(1179.80 ±606.33) ng/L] was significantly higher than those in simple pulmonary tuberculosis group [(727.53 ±511.66) ng/L,P ＜0.05],and they were both higher than those in healthy control group [ ( 352.50 ± 185.02 ) ng/L,P ＜ 0.05 ].Therefore,for further additional subgroup analysis in simple pulmonary tuberculosis group,the level of serum OPN in patients with cavity[ ( 836.51 ± 549.80) ng/L ] was significantly higher than that without cavity [ (535.52 ± 375.95 ) ng/L,P ＜ 0.05 ],while the levels of serum OPN had no statistical difference between pulmonary lesion involved ＜ 3 leaf patients and ≥ 3 leaf patients,sputum mycobacterium tuberculosis positive patients and negative patients,mycoDot test results positive patients and negative patients,erythrocyte sedimentation rate elevated patients and normal patients,PPD strongly positive patients and non-strongly positive patients (P＞0.05).ConclusionsOPN activity is closely related with pulmonary tuberculosis.OPN can serve as a judge of pulmonary tuberculosis and the clinical indicators of disease outcome.The level of serum OPN and the severity of pulmonary tuberculosis have some correlations.