1.Differences of T helper 17 cells and transforming growth factor-β1 between early and late primary biliary cirrhosis
Ting ZHANG ; Danxu MA ; Xi LI ; Qinghua MENG ; Fengchun ZHANG
Chinese Journal of Rheumatology 2015;19(8):507-511
Objective To explore the differences of Th17 population and serum transforming growth factor (TGF)-β1 levels between early-and late-stage primary biliary cirrhosis (PBC) and their roles in pathogenesis.Methods Peripheral Th17 counts were analyzed by flow cytometry.The expression of IL-17A in peripheral blood mononuclear cells and TGF-β1 were measured by real-time quantitative polymerase chain reaction.Serum concentration of TGF-β1 was measured by enzyme-linked immunosorbent assay.Liver biopsies were stained with hematoxylin-eosin to determine the pathological stage.Results were evaluated using KrustalWallis test followed by Mann-Whitney U tests for comparisons of Th17 population between patients with early and late PBC,patients with chronic hepatitis B (CHB) and health controls (HCs).ANOVA followed by LSD t-tests were used for comparing IL-17 mRNA,TGF-β1 mRNA and TGF-β1 serum concentration between groups.The correlations between Mayo risk score and peripheral Th17 of PBC patients,Mayo risk score and serum concentration of TGF-β1 was analyzed by Pearson correlation analysis separately.Results The peripheral Th17 population increased in patients with early PBC (1.03±0.33)%,compared to those with late PBC [(0.48± 0.13%,U=14.0,P<0.01],CHB [(0.56±0.35)%,U=104.5,P<0.01],and HCs [(0.36±0.17)%,U=8.0,P<0.01],while TGF-β1 changed in the opposite direction.Serum concentration of TGF-β1 elevated in late PBC (43.0± 18.7) ng/ml compared with early PBC (29.5±12.2) ng/ml,t=2.85,P=0.006.Conclusion The opposite changes of Th17 population and TGF-β1 level in early and late PBC indicated their different roles in different stages.Th17 may contribute to the autoimmune response in early PBC,participate in the occurrence of autoimmune inflammation,while TGF-β1 to fibrogenesis in late stage.In addition,the possible regulation mechanisms of differentiation of Th17 by TGF-β1 cannot be ignored.
2.Investigation and Comparison on Traditional Chinese Medicine Terminology Standards
Yuanbai LI ; Meng CUI ; Yang YANG ; Xiaobo ZHU ; Chaojie LIAN ; Meng LI ; Jing ZHANG ; Huaiping XI ; Hongming MA ; Na ZU
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(7):1592-1595
In this article, the Chinese Traditional Medicine and Materia Medica Subject Headings, Standards of the People's Republic of China - Classification and Codes of Diseases and Zheng of Traditional Chinese Medicine and the Chinese Terms in Traditional Chinese Medicine and Pharmacy were compared. Three standards were compared from the terminology quantity, content and classification. Each standard has its special feature. The compatibility and consistency are not strong in these standards. More authoritative traditional Chinese medicine terminology standards need to be established for the application in the clinical practice and scientific research.
3.Association between urinary polycyclic aromatic hydrocarbons metabolites and high-normal blood pressure in coke oven workers
MA Yi fei YAO Xi yuan WANG Hui min FU Meng meng WANG Yi dong NIE Ji sheng
China Occupational Medicine 2022;49(06):621-625
Objective ( )
To investigate the association between urinary polycyclic aromatic hydrocarbons PAHs metabolites
- Methods
and high normal blood pressure in coke oven workers. A total of 433 coke oven workers were selected as the study
-
subjects using convenient sampling method. They were divided into normal blood pressure group and high normal blood pressure
group according to their blood pressure level. The levels of ten kinds of urinary hydroxylated PAHs metabolites were measured by
- Results -
high performance liquid chromatography mass spectrometry. Among the subjects,57.5% had high normal blood
- , - , -
pressure. The levels of 1 hydroxynathalene 2 hydroxyphenanthrene 1 hydroxyphenanthrene and the metabolite of total PAHs
- ( P )
in the high normal blood pressure group were higher than those in the normal blood pressure group all <0.05 . The results of
- , - , - ,
the multivariate logistic regression analysis showed that urinary 1 hydroxynathalene 2 hydroxyfluorene 3 hydroxychrysene
- ( P ),
and metabolite of total PAHs were all risk factors for high normal blood pressure in coke oven workers all <0.05 after
, , , , ,
adjusting for confounding factors such as gender length of service body mass index smoking index alcohol consumption tea
, , , Conclusion
consumption night shift exercise frequency and other PAHs metabolites. Exposure to PAHs in coke oven
plants may increase the risk of elevated blood pressure within the normal range among coke oven workers.
5.Complementary treatment of 141 HIV/AIDS patients with pulmonary infection by qingfei peiyuan micro-pill: a clinical observation.
Xiu-Xia MA ; Li-Ran XU ; Zhi-Pan ZHENG ; Peng-Fei MENG ; Dong-Xu WANG ; Xi-Yuan SONG
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(8):960-963
OBJECTIVETo observe the effect of Qingfei Peiyuan Micro-pill (QPM) on HIV/AIDS patients with pulmonary infection of phlegm heat obstructing lung syndrome (PHOLS).
METHODSTotally 141 HIV/AIDS patients with pulmonary infection of PHOLS were randomly assigned to the treatment group (94 cases) and the control group (47cases). On the basis of Western medicine, patients in the treatment group took QPM. The therapeutic course for all was 28 days. The improvement of symptoms and signs was observed. The body temperature (BT), chest X ray, and white blood cells (WBCs) were detected.
RESULTSThe Chinese medical syndrome score was lower in the treatment group than in the control group at the 7th, 21st, and 28th day of treatment, showing statistical difference (P < 0.05). The efficacy was better in the treatment group than in the control group at the 7th, 21st, and 28th day of treatment, showing statistical difference (P < 0.05). The BT was lower in the treatment group than in the control group on the 7th day. There was no statistical difference in the patient number with normal WBCs on the 7th day (P > 0.05). But there was statistical difference in the patient number with normal WBCs on the 14th, 21st, and 28th day of treatment (P < 0.05). There was no statistical difference in the patient number with normal chest X ray on the 7th and 28th day of treatment (P > 0.05). But there was statistical difference in the patient number with normal chest X ray on the 14th and 21 st day of treatment (P < 0.05).
CONCLUSIONQPM had certain complementary effect on HIV/AIDS patients with pulmonary infection of PHOLS.
Acquired Immunodeficiency Syndrome ; complications ; Adult ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Respiratory Tract Infections ; complications ; drug therapy ; Treatment Outcome
6.The effect of 18β-sodium glycyrrhetinic acid on the nasal mucosa epithelial cilia in rat models of allergic rhinitis.
Jing YANG ; Kehu XI ; Yan GUI ; Youhu WANG ; Fuhong ZHANG ; Chunxia MA ; Hao HONG ; Xiangyi LIU ; Nannan MENG ; Xiaobing ZHANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(23):2060-2064
OBJECTIVE:
To investigate 18β-sodium glycyrrhetinic acid impact on nasal mucosa epithelial cilia in rat models of allergic rhinitis (AR).
METHOD:
AR models were established by ovalbumin-induction. Wister rats were randomly divided into groups as normal group, model group, budesonide (0.2 mg/kg) group and sodium glycyrrhetinic acid (20 mg/kg and 40 mg/kg) group after the success of AR models. At 2 weeks and 4 weeks after treatment, the behavioral changes of rats were observed and recorded, and nasal septum mucosae were collected after 2 week and 4 week intervention, and the morphological changes of nasal mucosae were observed by electron microscope.
RESULT:
Model group developed typical AR symptoms, the total score in all animals was > 5. With budesonide and sodium glycyrrhetinic acid treatment, the AR symptoms were relieved, and the total scores were reduced significantly (P < 0.01). Compared with the model group: after 2 weeks' intervention, thick mucous secretions on the top of columnar epithelium cilia in rat nasal mucosa was significantly reduced, and cilia adhesion, lodging, shedding were relieved in budesonide group and sodium glycyrrhetinic acid group, the relieve in budesonide group was slightly better than that in sodium glycyrrhetinic acid group; after 4 week intervention, Cilia adhesion, lodging, shedding were completely vanished, and the cilia were ranged in regular direction in budesonide group and sodium glycyrrhetinic acid group. Cilia in sodium glycyrrhetinic acid (20 mg/kg) group was more orderly, smooth than that in budesonide group and sodium glycyrrhetinic acid group (40 mg/kg), and the condition of cilia in sodium glycyrrhetinic acid group (20 mg/kg) was similar to the normal group.
CONCLUSION
18β-sodium glycyrrhetinic acid is effective to restrain the pathological changes of nasal mucosa cilia in rat models of AR.
Animals
;
Budesonide
;
pharmacology
;
Cilia
;
drug effects
;
Disease Models, Animal
;
Glycyrrhetinic Acid
;
analogs & derivatives
;
pharmacology
;
Nasal Mucosa
;
drug effects
;
Ovalbumin
;
Random Allocation
;
Rats
;
Rhinitis, Allergic
;
drug therapy
7.Analysis and countermeasures of adverse drug reactions of traditional Chinese medicine injections in children.
Xi-Lian ZHANG ; Meng LI ; Ping RONG ; Rong MA
China Journal of Chinese Materia Medica 2012;37(18):2807-2809
OBJECTIVEWe aimed to analyse the adverse drug reactions (ADR) in children due to traditional Chinese medicine (TCM) injections and the related factors, and explored the rational use of medicines and safty countermeasures in clinical.
METHODWe preformed statistical analyses on data from the CNKI and researched on literatures, from April 1987 to May 2012, relevant to the TCM injections which lead to reactions of clinical adverse, to conduct a analysis of the species, cases, clinical manifestations and related factors of these injections.
RESULTThe incidence of ADR in children leaded by TCM injections is high and the manifestations were chiefly characterized by the luscious of skin and appendages. In addition to the correlation with the physiological and pathologic characteristic in childhood, the ADR is also closely related with preparing technology, irrational use and imperfect supervisory system.
CONCLUSIONBecause of the high incidence, we should taking appropriate measures, such as constructing strict supervision system and strengthening rational drug use, to reduce the occurrence of ADRs to the greatest extent.
Child ; Child, Preschool ; Clinical Trials as Topic ; Drug-Related Side Effects and Adverse Reactions ; epidemiology ; Drugs, Chinese Herbal ; administration & dosage ; adverse effects ; Female ; Humans ; Male
8.Endovascular therapy for complex subclavian artery occlusive diseases
Zhu TONG ; Lian-rui GUO ; Jian-ming GUO ; xi-xiang GAO ; Tian-yu MA ; Meng-xia LIU ; Jian-xin LI ; Zhong-gao WANG ; Jian NG ZHA
Journal of Interventional Radiology 2015;(3):188-192
Objective To discuss the key points of endovascular therapy for complex subclavian artery occlusive diseases. Methods During the period from January 2012 to December 2013, a total of 92 patients with complex subclavian artery occlusive disease were admitted to Xuanwu Hospital of Capital Medical University, Beijing, China. The clinical data were retrospectively analyzed. The features of the lesions, the success rate of endovascular therapy, the use of combined approaches, the relief of symptoms after treatment, etc. were evaluated. Results The complex subclavian artery occlusive diseases could be divided into three types. Type Ⅰ: long segment of the left subclavian artery was occluded; type Ⅱ: ostial stenosis or occlusion of the right subclavian artery; and type Ⅲ: subclavian artery stenosis or occlusion was associated with the ostial disorder of the vertebral artery, or the opening of vertebral artery was affected by the subclavian artery stenosis or occlusion. The technical success rate was 82.6%. Combination use of femoral artery and brachial artery approach was employed in 27.2% of patients, which had improved the technical success rate. After the treatment the symptom improvement rate was 81.6%. Conclusion Upper limb artery approach can improve the re-canalization rate of left subclavian artery with long segment occlusion, and can ensure the accurate positioning of stent at the site of right subclavian artery opening. During the procedure of endovascular intervention for subclavian artery occlusion disease, attention should be paid to the protection of the vertebral artery.
9.Dysregulation of the TGF-beta postreceptor signaling pathway in cell lines derived from primary or metastatic ovarian cancer.
Ling XI ; Wei HU ; Li MENG ; Jianfeng ZHOU ; Yunping LU ; Changyu WANG ; Ding MA
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(1):62-65
Transforming growth factor-beta (TGF-beta) may cause cell cycle arrest, terminal differentiation, or apoptosis in most normal epithelial cells, whereas most malignant cell lines are resistant to TGF-beta. Mechanisms of resistance to TGF-beta caused by modulation of cell cycle regulators and/or inactivation of components of the TGF-beta signaling transduction pathway such as C-myc and Smad4 are not well understood. To investigate the potential association between loss of sensitivity to TGF-beta and expression status of transforming growth factor receptor II (TbetaR II) , Smad4, CDC25A and C-myc in 14 cell lines derived from ovarian cancer, the expression levels of these genes were detected by semi-quantitative RT-PCR. Normal ovarian surface tissues were used as controls. The expression of TbetaR II was detectable in all of 14 cell lines. The expression of Smad4 was decreased in 10 cell lines and 9 cell lines overexpressed CDC25A, as compared to normal controls. CDC25A gene was overexpressed with 88% (8/9) in tumorigenic cell lines as determined by xenografts in nude mice, and only in 20% (1/5) of non-tumorigenic cell lines (P<0.05). C-myc was not overexpressed in any of these cell lines. The loss of sensitivity to TGF-beta of cell lines derived from ovarian cancers may be related to a decreased expression of Smad4, which mediates TGF-beta induced growth inhibition, and/or an overexpression of CDC25A. This overexpression of CDC25A correlates with increased tumorigenicity of ovarian cancer cell lines. The loss of sensitivity to TGF-beta is not associated with a lack of TbetaR II.
Animals
;
Cell Line, Tumor
;
DNA-Binding Proteins
;
metabolism
;
Female
;
Humans
;
Mice
;
Mice, Nude
;
Ovarian Neoplasms
;
metabolism
;
pathology
;
Proto-Oncogene Proteins c-myc
;
metabolism
;
Receptors, Transforming Growth Factor beta
;
metabolism
;
Signal Transduction
;
Smad4 Protein
;
Trans-Activators
;
metabolism
;
Transforming Growth Factor beta
;
pharmacology
;
cdc25 Phosphatases
;
metabolism
10.Correlation Between Sleep Status and TCM Constitution Types in Escort Troops
Fengzhi WU ; Feng LI ; Jie MA ; Xicheng YU ; Ruqing XIE ; Yuehan SONG ; Weifeng WANG ; Meng MAO ; Chenxia HAN ; Siyuan GUO ; Xi YANG
Chinese Journal of Information on Traditional Chinese Medicine 2014;(6):24-26
Objective To explore the correlation between TCM constitution and sleep status in escort troops. Methods Standardized TCM Constitution Questionnaire and Pittsburgh Sleep Quality Index (PSQI) were used to investigate escort troops. Factors of PSQI and constitution types were analyzed. Results The results of PSQI showed the number of escort troops who had bad sleeping (PSQI>7) was 64, which accounted for 46%of the escort troops, among which, 45 people (70.3%) were justified as biased constitution, while 19 were normal constitution (29.7%). The top three biased constitution in bad sleeping were:qi deficiency, phlegm-dampness and dampness-heat. PSQI factors such as sleep disorder, daytime dysfunction and total score had positive correlation with qi deficiency, yang deficiency, yin deficiency, dampness-heat and blood stasis, and negative correlation with normal constitution. Conclusion Sleep condition of escort troops has correlation with TCM constitution, which indicates that sleep status could be improved by regulating TCM constitution.