Objective To investigate apoptosis in acinar cells during posttransplant reperfusion, and the expression of Bcl 2, Bax, Fas Methods Pancreas transplantation was performed between male SD rats after 2 hours of cold ischemia Rats ( n =65) were divided at random into 7 groups: sham group, cold ischemia group (as controls), reperfused 1 h, 3 h, 6 h, 9 h, 12 h group The pathologic pancreatic tissue was observed by electron microscope; Apoptotic cells were stained by TUNEL technique; Expression of Bax、Bcl 2 and Fas protein in pancreatic tissue were detected by SABC immunohistochemical method Results Apoptotic cells were observed in early phase after pancreas transplantation The peak of apoptotic pancreas cells occurred 3 hours after reperfusion [AI ( 9 5? 2 9)%, P

Objective:To establish the model of pancreaticoduodenal transplantation in rats. Methods:The enteric drained whole pancreas duodenal transplantation model was investisated. The donor aortic segment including the celiac, superior mesenteric divergence were anastomosed with recipient abdominal aorta with end to side. The donor portal was cuff-anastomosed with left renal vein. The graft duodenum were anastomosed with the host jejunum with end to side. Results:Eighty-nine operations were performed and 51 rats were successful, of which all graft of successful case had endocrinium function. Conclusion:This model for pancreas transplantation is reliable.

Acupressure ; Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Child ; Combined Modality Therapy ; Female ; Humans ; Male ; Spasm ; therapy ; Stomach Diseases ; therapy ; Young Adult

We report a case of retinal detachment in morning glory syndrome(MGS). We think that identification of the retinal break, removal of the traction force by vitrectomy, the use of long-acting gas as endotamponade, all contributed to the successful treatment of the disease. The pathogenic mechanisms of the disease and the function of B-scan to the diagnosis are included.

Objective To evaluate the early effect of hypoxemia on central executive function of working memory.Methods 24 patients with obstructive sleep apnea syndrome (OSAS) and 20 healthy matching controls were studied in probe retrieval task.Results In control group,both the low and high conflict identification tasks elicited the N270 component with right prefrontal [F4,(2.2?2.7) ?V]and posterior [P3,(7.0?3.0) ?V; P4,(6.9 ? 3.1) ?V] distribution. N430 was elicited in high conflict condition with right hemispheric lateralization. The positive component,P300( 300～400 ms) distributed remarkably at parietal scalp[P3,(7.0?3.0) ?V; P4,(6.9?3.1) ?V]was elicited only in identity identification task. The N270 was significantly decreased in the frontal regions in mild OSAS patients [F4,(5.0 ? 4.5) ?V]; F3,(6.0?4.5) ?V] in comparison with the controls( P

Objective In order to study the relationship between the platelet activation sign:P-selectin(GMP 140 ) expression and human IgA nephropathy.Methods Expression of platelet membrane glucoprotein Ⅱb/Ⅲa(GP Ⅱb ,GP Ⅲa ) in blood for IgA nephropathy patients(n=46) was investigated by flow cytometry methods.Results In IgA nephropathy the up-regulated GMP 140 expression in blood was significantly higher than that in normal humans.Conclusions GMP 140 might play an important role in pathogenesis of IgA nephropahty,and the level of GMP 140 in blood might predict renal outcome in IgA nephropathy.

Asthma ; diagnosis ; therapy ; China ; Humans ; Practice Guidelines as Topic ; standards

Airway Remodeling ; Asthma ; pathology ; physiopathology ; Child ; Humans

Objective To investigate the relationship of vascular endothelial growth factor(VEGF)with the invasion and metastasis of non-Hodgkin lymphoma.Methods The levels of serum VEGF was measured by enzyme linked-immunoadsorbent assay(ELISA).Comparing the parameters between NHL group and the control group.Compared the parameters with different groups divided by clinical stage,the effect of the treatment,the level of plasma LDH and effect of treatment.Analyzed the correlation of the levels of serum VEGF and plasma LDH in patients of NHL.Results The levels of serum VEGF are remarkablely higher in patients than that in the control group(P＜0.05).The levels of serum VEGF in the group of Ⅲ,Ⅳ clinical stage are higher than that in the group of Ⅰ,Ⅱ clinical stage,but no significant difference(P＞0.05)were observed.The level of serum VEGF is higher in the group of none CR than the group of CR(P＜0.05).The level of serum VEGF is higher in the group of non-normal level of plasma LDH than group of normal LDH level(P＜0.05).The levels of VEGF and LDH are significantly correlated each other(P＜0.01).Conclusion The angiogenesis are important for tumor metastasis in patients of NHL.The serum VEGF can be used as prognosis parameters in patients with NHL.

Objective The patients with non-small cell lung cancer ( NSCLC) are still at risk of brain metastasis after surgi-cal treatment.The study was to investigate the clinical characteristics and the risk factors for postoperative brain metastasis in patients with NSCLC in order to provide references for prophylactic cranial irradiation ( PCI) . Methods The clinical and prognostic data of 213 patients with NSCLC in our department of oncology from February 2008 to February 2012 were retrospectively analyzed.The occur-rences of postoperative brain metastasis in NSCLC patients with different characteristics were observed.Cox model was used to investi-gate the risk factors for postoperative brain metastasis in patients with NSCLC. Results The overall cumulative rate of brain metasta-sis was 23.9%(51/213) and the rates of brain metastasis at 1-year, 2-year, 3-year after surgery were 6.1%, 14.1%, 22.5% re-spectively.Univariate analysis showed that the rates of postoperative brain metastasis in NSCLC patients with different ages, histological types, tumor stages and CEA levels were statistically significant (P<0.05).Cox regression showed that adenocarcinoma (HR=2.00, 95%CI:1.11~3.64), lung cancer stageⅢ(HR=3.70, 95%CI:1.67~8.25) and high CEA (≥5.0μg/L)(HR=3.16, 95%CI:1.60~6.25) were risk factors for postoperative brain metastasis in patients with NSCLC.The survival time in NSCLC patients with brain metastasis was remarkably lower than that in patients without brain metastasis (P<0.05). Conclusion The NSCLC pa-tients with adenocarcinoma, III stage disease, or high CEA are more likely to develop brain metastasis after surgery, which should be the focus of PCI.