Objective To explore college students'achievement motivation level at basic medical education stage and its influencing factors.Method Achievement motivation scale and open questionnaire were applied in 223 college students at basic medical education stage.The effect of multiple influential factors on achievement motivation was analyzed from the perspectives of pursing for success and avoiding errors by multiple linear regression.Results The results showed that 70.4％ students had low achievement motivation level.Multiple factors had linear regression relation with Ms and Mf(RMs=0.644,RMf=0.898).Academic self assessment and learning attitude can increase the motivation for success significantly(P＜0.001).Performance evaluation and learning objective can significantly increase the motivation for avoiding errors(P＜0.001).Conclusion The study and application of achievement motivation can improve education quality at basic medical education stage.

AIM:To set up the quality standard for Fuyang Jiedu Granules(Radix Sutellariae,Herba Scute-(llariae) Barbatae,Herba Epimedii,etc.). METHODS: Radix Scutellariae,Herba Scutellariae Barbatae and Herba Epimedii in preparation were identified by TLC.The content of baicalin was determined by HPLC on Agilent Eclipse XBD-C_(18) column. The mobile phase was methanol-phosphoric-water(47∶53∶0.2).The detection wavelength was at 280 nm. A Shim-pack VP-ODS column was used to determine icariin.The mobile phase consisted of 0.32% phosphoric acid-acetonitrile(75∶25).The detection wavelength was 270 nm. RESULTS: The spot on the thin layer plate was clear.The negative contral was not disturbed.The linear range of baicalin was in the range 0.122 12 ?g to 0.606 ?g.The average recovery was 99.59% with RSD of 2.16%.The linearity range of icariin was in the range 0.201 6 ?g-1.008 ?g.The average recovery was 99.49% with RSD of 1.31%.(CONCLUSION:) The methods are specific,simple,fast and accurate,and can be used for quality control of Fuyang Jiedu Granules.

Alzheimer's disease( AD) , a neurodegenerative disease, can result in memory loss, cognitive and behavioral deficits. The pathological hallmarkes are β amyloid plaques and neurofibrillary tangles which lead loss of neurons in brain. As the extension of the central nervous system, retina has a similar tissue anatomy with central nervous system. The β amyloid plaques have also been detected in retina of AD. Furthermore, according to eye examinations of AD patients, we have found the loss of retinal ganglion cells, the attenuation of retinal nerve fiber layer thickness, the smaller changes of macula lutea, the decline of vascular density and so on. And then, there occurs the visual field loss and the decline of contrast sensitivity and so on in AD patients. Thus, the retina has occurred nerve degenerative changes in AD. Meanwhile, there has been proved that the retina nerve degeneration is even earlier than senile plaques formation in brain. In addition, curcumin, a natural and safe fluorescent dye, can be used to label β amyloid plaques in retina. The above suggests that retina can be a window for the early diagnosis of AD.

Objective: To investigate the probable mechanism of FSEER (ethanol extract of Forsythia suspensa root)-induced apoptosis of esophageal cancer TE-13 cells in vitro . Methods: Change of mitochondrial membrane potential in TE-13 celsl after treatment with different concentrations of FSEER (0.4, 0.5 and 0.6 mg/mL) for 24 h was examined by FCM (flow cytometry). The expressions of apoptosisassociated proteins [cleaved caspase-3, caspase-8, cleaved caspase-9 and Cyt-C (cytochrome-C)] in esophageal cancer cells after FSEER treatment were detected by Western blotting. The expression levels of Bax, Bad, Noxa, Bcl-2, Bcl-xl and Mcl-1 mRNAs in TE-13 cells after treatment with different concentrations of FSEER for 24 h were detected by RT (reverse transcription)-PCR. Results: FSEER (0.4, 0.5 and 0.6 mg/mL for 24 h) can significantly reduce the mitochondrial membrane potential of TE-13 cells in a dose-dependent manner, as compared with the TE-13 cells without any treatment (P < 0.05). The result of Western blotting showed that the expression levels of cleaved caspase-3, cleaved-caspase-9 and Cyt-C in TE-13 cells after FSEER treatment were significantly increased but the expression level of caspase-8 had no change as compared with those of the TE-13 cells without any treatment (P < 0.05). RT-PCR showed that FSEER could up-regulate the expression levels of Bax, Bad and Noxa mRNAs and down-regulate the expression levels of Bcl-2, Bcl-xl and Mcl-1 mRNAs. Conclusion: The underlying mechanism of FSEER-induced apoptosis of TE-13 cells may be related to the mitochondria-dependent pathway. Copyright © 2013 by TUMOR.

Female ; Humans ; Hyperkeratosis, Epidermolytic ; pathology ; Infant, Newborn

Breast Neoplasms ; pathology ; surgery ; Carcinoma, Ductal, Breast ; pathology ; surgery ; Cryoultramicrotomy ; Cytodiagnosis ; methods ; Female ; Histocytological Preparation Techniques ; methods ; Humans ; Intraoperative Care ; methods ; Precancerous Conditions ; pathology ; surgery

In this article some ideas on the discipline construction and development in the school of medicine of Shahghai Jiao Tong University were put forward based on the results analysis of state key discipline application in 2007 and comparison between approval and failure disciplines from team,research,graduates education and lab construction.It will be meaningful for the sustainable development of the school of medicine of Shahghai Jiao Tong University in future.

Objective To investigate the protective effect of ginsenoside Rb1 and Re on injury of the neonate rat cardiomyocyte induced by aconitine alkaloids.Methods The influence of aconitine and ginsenoside Rb1,Re together in neonate rat cardiomyocyte was observed respectively.The influence of these components in neonate rat cardiomyocyte was examined by myocard zymogram.The expression of Ca2+ channel gene Cav1.2 mRNA of neonate rat cardiomyocyte after treatment was observed by RT-PCR.Results The ginsenoside Rb1 and Re were able to decrease the release of AST and LDH,reverse Cav1.2 mRNA abundance induced by aconitine.Conclusion The ginsenoside Rb1 and Re which together with aconitine can alleviate the side effects and boost up the therapeutic effects of aconitine.The action mechanism may be relation with that the ginsenoside Rb1 and Re can decrease injuries of the neonate rat cardiomyocytes and abundant expression of Cav1.2 mRNA induced by aconitine.

Objective:The purpose of this study is to investigate the feasibility of labeling skin of inner mongolia cashmere goats with BrdU,and to optimize its immunohistochemical conditions.Methods:We injected different dose of BrdU into the neck vein of cashmere goats,then we took samples from the back skin of the goats,fixed in 4% paraformaldehyde.Next the samples were embedded with paraffin and sectioned.We examined the samples with immunohistochemical technology to test the effect of the influencing factors to labeling results as the BrdU injection,such as dosage,the concentration of second antibody and streptavidin-peroxidase,the way of antigen pretreatment,and DNA denature temperature.Results:We observed red BrdU positive cells in labeled goats whereas not in the control.Meanwhile,in the samples of the unlabeled goats or those incubated with PBS,no nuclear staining is observed.Conclusion:BrdU can be used for labeling of inner mongolia cashmere goats in vivo,which have no obvious dosage effect.The best condition of labeling is that puttig the section in 2 mol/L HCl 30 min at 37℃,in trypsin 30 min at 37℃.The most appropriate concentration of biotin-conjugated second antibody and streptavidin-peroxidase is 1∶100,under which the results of immunohistochemical are the best.

Objective To investigate the status of clinical using of blood products in Shanghai area,clinicians' understanding on transfusion knowledge and consciousness of prevention of transfusion risk.Methods Two hundreds of clinicians who were randomly selected from level two and above hospitals of Shanghai were inquired by questionnaire.Data including understanding of transfusion techniques,prevention of transfusion risk,application of blood components and grasp of new techniques were collected and analyzed.Results Among all interviewed clinicians,those who realized the importance of transfusion on clinical work,who understood the basic contents of clinical guideline for transfusion issued by Ministry of Health People's Republic of China,and who had the basic knowledge of blood component products and transfusion applications account for 62%,60% and 81% respectively.But only 28% clinicians knew all the side effects,27.3% learned the new techniques of transfusion,including leukocyte reduced,virus elimination,clinical application of irradiation.This investigation also showed 97% clinicians would like to accept training of transfusion,68% thought they need more relevant information about prevention of transfusion risk.Conclusions Clinicians in Shanghai area had the basic knowledge of clinical guideline for transfusion,but some knowledge of transfusion were insufficient including blood components and their applications,side effects and new techniques of transfusion.Clinicians in Shanghai had active response for being trained on knowledge of transfusion.