Setting Chinese as preparatory courses is an effective way to harmonize Chinese language teaching and medical teaching.By this way,some low-level students can be disqualified from further study,thus both the level of Chinese teaching and medical professional teaching can be improved.In the process of the eductional system reform from 5 years to 6 years,building Chinese training based preparatory course,will consummate the oversea medical undergrade education system.

After an analysis of the urgent need of systematic medical information service in village and township hospitals and doctors and the advantageous conditions for medical academic libraries to provide information service for them, its imple-mentation strategies were proposed and how to promote the construction of libraries and the development of medical cause in villages and townships by providing information service in countryside were pointed out.

Genotype ; Hepatitis B, Chronic ; complications ; Hepatitis C, Chronic ; complications ; Humans ; Parvoviridae Infections ; complications ; virology ; Parvovirus ; classification ; genetics ; isolation & purification ; Parvovirus B19, Human ; isolation & purification

Objective To observe the clinical effects of surgical treatment of retinal detachment(RD) caused by macular hole(MH) in high myopia. Methods The clinical materials of 149 eyes of 149 high myopia patients with RD caused by MH were reviewed. The cases were divided into complete posterior vitreous detachment (PVD) group and incomplete PVD group. The anatomic successful rate of operative treatment was evaluated according to the applications of vitrectomy surgery and non vitrectomy surgery respectively in each group. The visual acuity changes after the operations were also observed. Results The anatomic successful rates were as follow: 77.9% in total cases with vitrectomy surgery and 25.9% with non vitrectomy surgery ( P 0.05). Conclusions The scleral buckling combined with vitrectomy, gas intraocular tamponade and postoperative photocoagulation is an effective and optimal procedure for RD caused by MH in high myopia.

Objective To investigate apoptosis in acinar cells during posttransplant reperfusion, and the expression of Bcl 2, Bax, Fas Methods Pancreas transplantation was performed between male SD rats after 2 hours of cold ischemia Rats ( n =65) were divided at random into 7 groups: sham group, cold ischemia group (as controls), reperfused 1 h, 3 h, 6 h, 9 h, 12 h group The pathologic pancreatic tissue was observed by electron microscope; Apoptotic cells were stained by TUNEL technique; Expression of Bax、Bcl 2 and Fas protein in pancreatic tissue were detected by SABC immunohistochemical method Results Apoptotic cells were observed in early phase after pancreas transplantation The peak of apoptotic pancreas cells occurred 3 hours after reperfusion [AI ( 9 5? 2 9)%, P

OBJECTIVE: To investigate the effect of genistein on cell proliferation and apoptosis in human laryngeal carcinoma cell line Hep-2. METHOD: Cell Counting Kit-8 (CCK-8) assay was used to measure the 50% inhibiting concentration (IC50) value of genistein; cell apoptosis rate and the distribution changes of cell cycle were determined with flow cytometry assay after treatment by gensitein. The morphological changes of tumor cells were evaluated by inverted phase contrast mircroscopy. RESULT: The IC50 of geniste responses to Hep-2 cells for 24 h was 23.64 µg/ml. The apoptotic rates of Hep-2 cells treated by genistein for 24 h were 22.40% ± 1. 65% (at 12 µg/ml genistein) and 30.64% ± 2.94% (at 24 µg/ml genistein) respectively, significantly statistical differences were foundbetween above threated groups and the control group (P < 0.05); the apoptotic rates of Hep-2 cells treated by genistein for 48 h were 30.55% ± 0.72%(at 12 µg/ml genistein) and 48.69% ± 1.06% (at 24 µg/ml genistein) respectively, significantly statistical differences were found between above threated groups and the control group (P < 0.05). When Hep-2 cells exposed to the same concentration of genistein for 24 h, 48 h respectively, the difference in apoptotic rate was statistically significant. CONCLUSION Genistein inhibited Hep-2 cells growth obviously, meanwhile it could induced apoptosis of Hep-2 cells, the apoptotic rate was increasing with the increase of the time and dose of genistein.
Apoptosis ; Cell Cycle ; Cell Line, Tumor ; drug effects ; Cell Proliferation ; Flow Cytometry ; Genistein ; pharmacology ; Humans ; Laryngeal Neoplasms ; pathology

Objective　To study the ultrastructure of macular pucker (MP) from the patients with rhegmatogenous retinal detachment (RRD) and the mechanism associated with its development．　Methods　Twenty specimens of MP surgically removed by vitrectomy from 13 patients were dissected into two layers in each of them．The ultrastructure of two layers,i,e,near the vitreous and near the retina,was studied with electron microscopy．　Results　Seven sections of the near vitreous ones appeared prodominant collagen deposits and a few of epithelial-like cells,and pigment particles might be present in the cytoplasm．While cells with foot processes were found in 13 membrane sections near the retina and increasing number of various types of cells rich in collagen around were observed including fibroblast-like cells and glial cells．　Conclusion　The findings suggest that the MP after surgery of retinal detachment may possess a characteristic lamination,and posterior hyaloid cortex was involved in the developmetn of MP．The adhesion between posterior hyaloid cortex and macular area might be a key factor for forming MP．

This paper summarizes the observation and nursing of patients with postoperative complications following percuta-neous transhepatic cholangogrphic drainage(PTCD) and biliary tract stent implantation for malignant obstructive jaundice. A-mong thirty-two patients,eight had postoperative complications,including two with biliary tract hemorrhage,four with biliary tract infection,one with pneumothorax and one with bile leakage. All the thirty-two patients recovered well in clinical symptoms and quality of life. To attach importance to psychological nursing and postoperative health education are the key points in nursing care of patients with PTCD,which play important roles in alleviating clinical symptoms,improving treatment outcome and pro-moting the patients' quality of life.

Objective:To establish the model of pancreaticoduodenal transplantation in rats. Methods:The enteric drained whole pancreas duodenal transplantation model was investisated. The donor aortic segment including the celiac, superior mesenteric divergence were anastomosed with recipient abdominal aorta with end to side. The donor portal was cuff-anastomosed with left renal vein. The graft duodenum were anastomosed with the host jejunum with end to side. Results:Eighty-nine operations were performed and 51 rats were successful, of which all graft of successful case had endocrinium function. Conclusion:This model for pancreas transplantation is reliable.

Acute Disease ; Humans ; Pneumonia ; prevention & control ; Pulmonary Disease, Chronic Obstructive ; complications ; Respiration, Artificial ; Respiratory Insufficiency ; therapy ; Ventilators, Mechanical ; adverse effects