The newly established genus Pseudoalteromonas has attracted significant interest of more and more researchers by their unique biological characteristics.In this review ,the classification and ecological distribution of Pseudoalteromonas were briefly discussed,then special emphasis placed on the extracellular bioactive agents and their bioactivity of Pseudoalteromonas.Some ideas for further studies on Pseudoalteromonas were also proposed .

Objective To investigate the effect and mechanism of Src kinase in renal interstitial fibrosis of unilateral ureteral obstruction (UUO) mice.Methods Male C57BL/6J mice were randomly divided into 4 groups,including sham operation group (n=8),sham operation+PP2 group (n=8),UUO operation group (n=8) and UUO operation+PP2 group (n=8).The mice were injected 2 mg/kg PP2 by intraperitoneal everyday after surgery in sham+PP2 group and UUO+PP2 group.PP2 dissolved in 1％ DMSO (formulated with normal saline).Sham and UUO group were given equal 1％ DMSO.The mice were sacrificed at 7th day.Renal collagen was observed with Sirius red stain.The activities of Src,protein kinase B (PKB,AKT),p38 mitogen-activated protein kinase (p38 MAPK),extracellular signal-regulated kinase (ERK) and the protein expressions of α-smooth muscle actin (α-SMA) and fibronectin (FN) were detected by Western blotting.The expression of collagen I (COL [) was detected by immunohistochemistry and the expressions of matrix metalloprotein 9 (MMP-9),tissue inhibitor of metalloproteinase 1 (TIMP-1),transforming growth factor-β31 (TGF-β31),monocyte chemotactic protein-1(MCP-1),interleukin-6 (IL-6) were measured by ELISA.Resuts Compared with sham mice,UUO mice on 7th day displayed obvious renal fibrosis.Meanwhile,UUO mice had increased expressions of COL Ⅰ and FN,and activities of AKT,ERK and p38 MAPK (all P ＜ 0.05).Their renal expressions of α-SMA,TGF-β1,MMP-9,TIMP-1,MCP-1 and IL-6 were also raised (all P ＜ 0.05).Compared with those in UUO group,in UUO + PP2 group the activities of Src,AKT,p38 MAPK and ERK,and expressions of TGF-β1,MCP-1 and IL-6 decreased (all P ＜ 0.05).Additionally,expressions of COL Ⅰ,FN and α-SMA,collagen deposition and renal fibrosis receded in UUO + PP2 group (all P ＜ 0.05).However,the expressions of MMP-9 and TIMP-1 were not influenced by PP2 treatment.Conclusions Src kinase promotes myofibroblasts accumulation and inflammatory reaction through activating its downstream signaling pathway in the progressing of renal interstitial fibrosis.

Objective To investigate the relationship between metabolic syndrome and related factors and recurrence of ischemic stroke.Methods 90 in-and out-patients with ischemic stroke in Jiangbei District Hospital of Traditional Chinese Medicine during May 2013 and May 2014 were enrolled.In 90 cases,4 patients with cerebral hemorrhage were excluded.Basic data of the 86 patients were investigated,which including gender,age,abdominal circumference,smoking,drinking,family history of stroke,diabetes,hypertension,hyperlipidemia,a history of atrial fibrillation,transient ischemic attacks,fasting blood glucose,triglyceride,cholesterol,uric acid,homocysteine,urinary protein-creatine ratio,carotid ultrasound examination.We investigated patients with or without recurrent stroke during one year of telephone visit and follow-up.Patients with recurrent stroke were investigated.Results During the one year follow-up,24 cases in the 86 patients with ischimic stroke were recurrent,the recurrence rate was 27.9 ％.2 cases in the recurrent patients died because of massive cerebral infarction,accounted for 8.3％ of the patients with recurrent stroke.According to Chi-square test,smoking,high fasting plasma blood glucose,hypertension,elevated low density lipoprotein cholesterol,high uric acid,high homocysteine and carotid atherosclerosis all had correlation with recurrent stroke(P＜0.05).In this logistic-regression model,smoking,high fasting plasma blood glucose,hypertension,elevated low density lipoprotein cholesterol,high uric acid,high homocysteine and carotid atherosclerosis were all predictors for recurrence of ischemic stroke.Conclusion Cntrolling the risk factors after the first stroke as a long term strategy has important clinical significance for secondary prevention of ischemic stroke.

Costs and Cost Analysis ; Dentists ; Family Practice ; Humans ; Physician-Patient Relations ; Private Practice ; economics ; standards ; Social Values

Carcinoma ; Cytological Techniques ; Humans ; Nasopharyngeal Neoplasms ; pathology ; Neoplastic Stem Cells ; cytology ; Oncogenes

OBJECTIVE: To synthesize folic acid-modified dextran-5-fluorouracil (FA-DEX-5-Fu) conjugates and investigate its cytotoxicity on cells with folate receptors. METHODS: Dextran (DEX) was selected as the macromolecular carrier and the anti-cancer drug 5-fluorouracil (5-Fu) was selected as a model drug to synthesize 5-Fu-DEX, and then folic acid (FA) was linked into 5-Fu-DEX as the targeting ligand to obtain the conjugate, FA-DEX-5-Fu. UV spectra, IR and ESI-MS were used to identify the structure. UV spectrophotometry was used to determine the contents of FA and 5-Fu in the conjugate. MTT assay was applied to examine the cytotoxicity of free 5-Fu, 5-Fu-DEX and FA-DEX-5-Fu on the Human cervical carcinoma cell line Hela (overexpressing folate receptors) and human lung carcinoma cell line A549 (folate receptor deficient, as negative group). RESULTS: 5-Fu and FA were successfully linked with 5-Fu-DEX as confirmed by UV, IR and ESI-MS spectra.The molar ratio of 5-Fu and FA in the conjutate was 2.7:1 as determined by UV spectrophotometry. MTT assay results showed the inhibition rate of FA-DEX-5-Fu on Hela cells was much higher than that on A549 cells. And the inhibition rate of FA-DEX-5-Fu on Hela cells was much higher than those of 5-Fu-DEX and free 5-Fu at the same concentration. CONCLUSION: FA-DEX-5-Fu is successfully synthesized and the conjugate shows significant targeting effect against tumor cells overexpressing folate receptors.

Child ; DNA, Antisense ; genetics ; DNA, Viral ; genetics ; Genetic Therapy ; methods ; Humans ; RNA, Antisense ; genetics ; Respiratory Syncytial Virus Infections ; therapy ; Respiratory Syncytial Virus, Human ; genetics ; pathogenicity

Sperm DNA fragmentation index (DFI) refers to the percentage of DNA strand breaks in the total sperm. Many studies suggest that elevated DFI can lead to male infertility and early spontaneous abortion. High-DFI patients are more likely to fail in assisted reproduction and preliminary treatment or prevention methods have been developed for this population. This review focuses on the impact of DFI on clinical pregnancy outcomes and progress in the studies of its treatment.
Abortion, Spontaneous ; Chromatin ; DNA Fragmentation ; Female ; Humans ; Infertility, Male ; Male ; Pregnancy ; Pregnancy Outcome ; Sperm Injections, Intracytoplasmic ; Spermatozoa

Cardiovascular Diseases ; diagnosis ; genetics ; Humans ; MicroRNAs ; blood ; genetics

Air Pollutants, Occupational ; analysis ; Carbon Tetrachloride ; analysis ; Chromatography, Gas ; Environmental Monitoring ; methods ; Workplace