The complications of extracorporeal membrane oxygenation ( ECMO ) include technical relevance complications and physical relevance complications. The former mainly includes dysfunction of oxygenation,cannula-related problems,technical relevance hemolysis,blood clots of oxygenator or rest of the circuit. The latter includes hemorrhage and embolism,culture-confirmed infection,renal failure and neurologic complications. One of the most reported complications of ECMO is hemorrhage. It′s important for reducing mortality and improving prognosis through stricting with the indications of ECMO,strengthening team cooper-ation,closely clinical monitoring and early identifying complications. In this paper,we introduced complica-tions of ECMO as well as how to prevent and treat it.

Malnutrition occurs as a result of inadequate nutrient intake,malabsorption and altered me-tabolism in pediatric liver failure. Malnutrition may be associated with a poor outcome. The current evidence indicates that the provision of adjunctive nutritional support ( parenteral nutrition or enteral nutrition or nutri-tional supplements) to patients with a variety of liver diseases. It may be reasonable to start enteral nutrition in 5 to 7 days in acute liver failure or hepatic coma. According to individualized appropriate nutritional evalu-ation,the metabolic demand,to provide appropriate nutritional support in pediatric liver failure.

Objective To observe the relationship of the Janus kinase-signal transducers and activator of transcription pathway and neurogenesis in the lateral ventricle subventricular zone(SVZ) and choroid plexus of neonatal rats with periventricular leukomalacia(PVL).Methods PVL models were established by right common carotid artery ligation followed by 4 h 60 mL/L oxygen exposure in 2-day-rat;the neonatal rats performed a sham operation,without hypoxia-ischemia were used as control group.The rats were sacrificed at 0 h,3 h,6 h,12 h,1 d,3 d and 7 d after hypoxic-ischemic(HI),and brain tissues were collected,immunohistochemistry was used to detect the expression of P-STAT3 and Nestin in the subventricular zone and choroid plexus.Results Very low expressions of P-STAT3 and Nestin were observed in lateral ventricle SVZ and choroid plexus in control group.The expression levels of P-STAT3 and Nestin increased significantly after HI,peaked at 1 d and 3 d separately,and remained at a higher level at 7 d after HI,demonstrating significant differences at each time point compared with those in control group(Pa

Adipose Tissue ; Animal Feed ; Animals ; Hypothalamus ; drug effects ; physiology ; Leptin ; pharmacology ; Male ; Mice ; Mice, Inbred Strains ; RNA ; analysis

Objective To study the effect of amelogenin (AML)on the migration,adhesion and proliferation of periodontal ligament stem cells (PDLSCs).Methods STRO-1 +PDLSCs were selected by Flow cytometer.The transwell system was used to detect the effect of AML on migration of PDLSCs.Cells attachment assay was used to detect the effect of AML on adhesion of PDLSCs.MTT was used to detect the effect of AML on the proliferation of PDLSCs.Results The results of transwell showed that AML could induce the migration of PDLSCs with positive correlation.Cells attachment assay showed that the adhesion of PDLSCs was effected by AML as well,and the effect was in-creased with the extension of incubation time.Results of MTT showed that AML could promote the proliferation of PDLSCs in a dose depend-ent manner.Conclusion AML could increase the proliferation,adhesion and migration potential of PDLSCs.

OBJECTIVE:To prepare ambroxol hydrochloride orally disintegrating tablets and to evaluate its quality.METHODS:Mannitol was used as main adjuvant to prepare ambroxol hydrochloride orally disintegrating tablet,the UV spec-trometry was adopted to determine ambroxol content,the disintegration time,taste and dissolution rate were evaluated.RESULTS:The optimal formulation was composed of MCC 50g,mannitol 20g,L-HPC 10g,and NaHCO3 2g;The detected concentration of ambroxol was linear in the range of 0.005～0.030mg/ml(r=0.9 999),the average recovery was 100.50%and RSD=0.71;The prepared tablets disintegrated completely within 30s.CONCLUSION:The orally disintegrating tablets is quick to disintegrate and is as effective as market tablets.

Humans ; Muscular Dystrophy, Duchenne ; Research ; trends

Objective To provide reference for improving the performance of public health by establishing performance evaluation framework of public health in rural areas of China.Methods The performance evaluation framework of public health in rural areas is established by literature research,theoretical derivation and Delphi method.The results of expert consultation are tested by W test of Kendall coordination coefficient and chi-square test.Results The performance evaluation framework of public health in rural areas contains four conceptual modules,ten performance goals and twenty-eight performance evaluation indexes.The positive coefficient of experts is 0.889.The average values of authoritative grade of experts are between 0.681～0.715.The Kendall W uniformity coefficients of the necessity,sensitivity and maneuverability of the indexes are respectively 0.167,0.209 and 0.185.The P values are less than 0.01.Conclusion The performance evaluation framework of public health in rural areas has certain application value.It is necessary to pay attention to scientific nature and applicability in developing performance evaluation of public health in rural areas.

Objective To investigate the accuracy of three dimensional facial measurement system based on structured light projection, and explore the methods to reduce noise of the output images. Methods The known object for calibration was measured by the measurement system to correct the parameters of the system and enhance the measurement accuracy. The mechanism of noise was analysed, and the noise and sundry of the images were eliminated. Results The parameters of each assembly of system were obtained by calculation and calibrated, and the measurement accuracy (0.028 mm) of three dimensional facial measurement system based on structured light projection was increased. Application of image processing technology reduced the noise and sundry of output images. Conclusion The accuracy of three dimensional facial measurement system based on structured light projection is high. The image output is reliable, and can be clinically used in facial scanning and three dimensional reconstruction.

Objective To understand the current situation and requirements of laboratory quality control of blood diseases in Chongqing .Methods A self‐designed questionnaire was designed ,and the investigation was carried out in 60 hospitals of Chongqing district .The main contents of the investigation were related to the instruments of work environment ,the regulations ,the conditions of the personnel ,etc .Results A total of 60 questionnaires were distributed ,57 questionnaires were valid .In the study ,only 14 .04%laboratories were the blood disease specialist laboratory ,most hospitals could complete the basic inspection items .66 .67% hospitals had all the experimental procedures and SOP documents .Most of staffs were with intermediate professional titles and undergraduate education .A total of 35 .08% experimental equipments with the value of over 5 million RMB ,45 .61% equipments had a special in‐spection records .About 45 .61% test reports would refer to the clinical performance ,and had a strict signing guideline .Most of the research objects reflected the problems ,including the backward equipments and the lack of personnel ,and hoped to set up telemedi‐cine indemnity system and carry out further education .Conclusion The overall quality of the laboratory for blood diseases in Chongqing is better ,it is recommended to strengthen the full quality control ,to establish emergency system ,and to improve the quality and level of laboratory .