Lung cancer is one of the most common malignant tumors in the world.Non-small lung canc-er( NSCLC) accounts for approximately 85%,but its pathogenesis has not been elucidated.As a family of soluble enzymes,matrix metalloproteinases(MMPs)can degrade the constituents of the extracellular matrix(ECM)and the basement-membrane,members share common structural features and are responsible for the relative specific sub-strates.Some researches show that the matrix metalloproteinases 7(MMP-7)involve in the invasion and metasta-sis of NSCLC,the expression of MMP-7 in the tissue of NSCLC is significcantly higher than that in adjacent tis-sues,and the MMP-7 plays important roles in prognosis evaluation of NSCLC patients.Therefore this paper sum-marize the domain structure,function,regulation and research in NSCLC about MMP-7.

A novel exonuclease protection mediated PCR assay (EPM-PCR) to detect the interaction of protein and DNA at a dioxin-responsive enhancer (DRE) upstream of the CYP1A1 gene in rat hepatic cytosol was established. A double-stranded DNA fragment containing two binding sites was designed and incubated with the aryl hydrocarbon receptor (AhR) transformed by 2,3,7,8-tetrachlorodibenzo-p dioxin (TCDD) to generate TCDD: AhR: DNA complex which could protect receptor-binding DNA against exonuclease II (Exo III) digestion. With Exo III treatment, free DNAs were digested and receptor-bound DNAs remained that could be amplified by PCR. By agarose gel electrophoreses a clear band (285bp) was detected using TCDD-treated sample, while nothing with control samples. To detect transformed AhR-DRE complex, 2 fmol DNAs and 3 ug cytosol proteins were found to be sufficient in the experiment. Compared with gel retardation assay, this new method is more sensitive for monitoring the Ah receptor-enhancer interaction without radioactive pollution.
Binding Sites ; Cytochrome P-450 CYP1A1/*genetics ; Cytosol/metabolism ; DNA-Binding Proteins/chemistry ; Exodeoxyribonucleases/chemistry ; Liver/*metabolism ; Polymerase Chain Reaction ; Rats, Sprague-Dawley ; Receptors, Aryl Hydrocarbon/*chemistry ; Tetrachlorodibenzodioxin/*analogs & derivatives ; Tetrachlorodibenzodioxin/chemistry

Intestinal trefoil factor(IFF) is a low molecular weight polypeptide expressed in intestine.With the research on its structure and physiological function,ITF is beginning to attract attention.This review is mainly about the structure,localization,function,mechanism of action,expression regulation,signal transduction pathway,recombinant expression and so on.

Humans ; Hypertension ; Sympathetic Nervous System ; metabolism ; pathology

Objective: To observe the changes of serum adiponectin level in patients with non-alcoholic fatty liver disease (NAFLD),and to study the correlation between the serum level of adiponectin and NAFLD. Methods: A total of 110 patients with NAFLD who were hospitalized from Feb. 2006 to Mar. 2008 were included in this study. The patients were divided into non-alcoholic steatohepatitis (NASH) group (n=52,group 1) and simple fatty liver group (n=58,group 2). Fifty-four healthy volunteers were taken as normal control (group 3). The height, weight, and body mass index (BMI) were measured in all groups. The fasting blood samples were obtained to examine serum adiponectin, TC, TG, HDL-C and LDL-C. Stepwise regression method was used to analyze relationship between the serum level of adiponectin and NAFLD. Results: The serum adiponectin and HDL-C levels in group 1 and group 2 were significantly lower than those in group 3 (P<0.01); the levels of TC,TG,and LDL-C were significantly higher than those of group 3 (P < 0.01). The serum adiponectin in group 2 was significantly lower than that in group 1 (P<0.05). Lower serum adiponectin and HDL-C levels (P<0.05) and higher AST level (P<0.01) were found in group 2 than those in simple fatty liver patients. In non-alcoholic steatohepatitis patients,serum adiponectin level was negatively correlated with the levels of TC, TG (r = - 0.436, P < 0.05; r = - 0.567, P < 0.01, respectively) and positively correlated with the level of HDL-C (r=0.524,P<0.01). Conclusion: The serum adiponectin level is correlated with the severity of NAFLD patients, and examination of serum adiponectin may be helpful in understanding the status of NAFLD patients.

Objective: To investigate the chemical constituents from Peucedanum decursivum. Methods: P. decursivum was extracted with 95% ethanol, the compounds were isolated via various chromatographic methods, and their structures were identified by detailed spectroscopic analyses. Results: Twelve compounds were isolated from the acetic ether fraction in the 95% extract of P. decursivum and were identified as isobergapten (1), bergapten (2), pimpinellin (3), isopimpinellin (4), columbianetin acetate (5), sphondin (6), qianhucoumarin E (7), xanthotoxin (8), osthole (9), ferulic acid (10), β-sitosterol (11), and psoralen (12). Conclusion: Compounds 1, 4, and 5 are isolated from the plants in Peucedanum L. for the first time, and compounds 2, 3, 6-10, and 12 are firstly isolated from P. decursivum.

Differential diagnosis of benign and malignant ascites is of a great value in determining etiology, evaluating prognosis, and determining therapy regimens. There are many diagnostic methods for ascites, but up to now, no method can achieve high sensitivity and specificity and meet the requirements of clinical economics and feasibility at the same time. Therefore, searching for an ideal method for differential diagnosis of ascites has become an important topic of current research. This article describes the clinical value of diagnostic methods, such as molecular biological detection, cytological examination, combined determination of tumor markers, and routine tests, in the differential diagnosis of benign and malignant ascites, analyzes the diagnostic efficiency of related biomarkers, and points out that combined determination of various parameters has an increased value in the differential diagnosis of benign and malignant ascites.

Acupuncture Points ; Acupuncture Therapy ; Humans ; Male ; Middle Aged ; Raynaud Disease ; therapy

Humans ; Infant, Newborn ; Solanaceous Alkaloids ; poisoning

Objective To investigate the ultrastructure changes of pancreatic islets alpha cells in rats with impaired glucose tolerance.Methods 16 Wistar rats were randomly assigned into normal glucose tolerance group(NGT group,n =8)and impaired glucose tolerance group(IGT group,n =8).NGT group were fed with routine diet and IGT-group were fed with high-sugar high-fat diet.At the 12th week,IGT models were considered successful if the level of 2-hour peripheral blood glucose(2 h PG)was between 7.8 and 11.1 mmol/L in oral glucose tolerance test and continued for more than a week.The pancreas tail of animals in the NGT group and IGT group rats was removed for ultrastructure observation.Alpha cells were observed under transmission electron microscope.Results The big-plump secretory granule with aureole appeared in pancreatic islets alpha cells of NGT group,and it contained a round dense core.The nuclear chromatin and contents were evenly distributed.Mitochondria and en-doplasmic reticulum arranged tightly and orderly.Compared with NGT group,the quantities of secretory granule increased significantly in IGT group.The gap between dense core and membrane was narrower.The quantities of mitochondria and rough endoplasmic reticulum increased significantly.There was no significant change for the structure of the other cell organelle.Conclusion The pathogenesis of IGT is associated with ultrastructural changes and dysfunction of pancreas islets alpha cells.