Lung cancer is one of the most common malignant tumors in the world.Non-small lung canc-er( NSCLC) accounts for approximately 85%,but its pathogenesis has not been elucidated.As a family of soluble enzymes,matrix metalloproteinases(MMPs)can degrade the constituents of the extracellular matrix(ECM)and the basement-membrane,members share common structural features and are responsible for the relative specific sub-strates.Some researches show that the matrix metalloproteinases 7(MMP-7)involve in the invasion and metasta-sis of NSCLC,the expression of MMP-7 in the tissue of NSCLC is significcantly higher than that in adjacent tis-sues,and the MMP-7 plays important roles in prognosis evaluation of NSCLC patients.Therefore this paper sum-marize the domain structure,function,regulation and research in NSCLC about MMP-7.

A novel exonuclease protection mediated PCR assay (EPM-PCR) to detect the interaction of protein and DNA at a dioxin-responsive enhancer (DRE) upstream of the CYP1A1 gene in rat hepatic cytosol was established. A double-stranded DNA fragment containing two binding sites was designed and incubated with the aryl hydrocarbon receptor (AhR) transformed by 2,3,7,8-tetrachlorodibenzo-p dioxin (TCDD) to generate TCDD: AhR: DNA complex which could protect receptor-binding DNA against exonuclease II (Exo III) digestion. With Exo III treatment, free DNAs were digested and receptor-bound DNAs remained that could be amplified by PCR. By agarose gel electrophoreses a clear band (285bp) was detected using TCDD-treated sample, while nothing with control samples. To detect transformed AhR-DRE complex, 2 fmol DNAs and 3 ug cytosol proteins were found to be sufficient in the experiment. Compared with gel retardation assay, this new method is more sensitive for monitoring the Ah receptor-enhancer interaction without radioactive pollution.
Binding Sites ; Cytochrome P-450 CYP1A1/*genetics ; Cytosol/metabolism ; DNA-Binding Proteins/chemistry ; Exodeoxyribonucleases/chemistry ; Liver/*metabolism ; Polymerase Chain Reaction ; Rats, Sprague-Dawley ; Receptors, Aryl Hydrocarbon/*chemistry ; Tetrachlorodibenzodioxin/*analogs & derivatives ; Tetrachlorodibenzodioxin/chemistry

Intestinal trefoil factor(IFF) is a low molecular weight polypeptide expressed in intestine.With the research on its structure and physiological function,ITF is beginning to attract attention.This review is mainly about the structure,localization,function,mechanism of action,expression regulation,signal transduction pathway,recombinant expression and so on.

Objective: To observe the changes of serum adiponectin level in patients with non-alcoholic fatty liver disease (NAFLD),and to study the correlation between the serum level of adiponectin and NAFLD. Methods: A total of 110 patients with NAFLD who were hospitalized from Feb. 2006 to Mar. 2008 were included in this study. The patients were divided into non-alcoholic steatohepatitis (NASH) group (n=52,group 1) and simple fatty liver group (n=58,group 2). Fifty-four healthy volunteers were taken as normal control (group 3). The height, weight, and body mass index (BMI) were measured in all groups. The fasting blood samples were obtained to examine serum adiponectin, TC, TG, HDL-C and LDL-C. Stepwise regression method was used to analyze relationship between the serum level of adiponectin and NAFLD. Results: The serum adiponectin and HDL-C levels in group 1 and group 2 were significantly lower than those in group 3 (P<0.01); the levels of TC,TG,and LDL-C were significantly higher than those of group 3 (P < 0.01). The serum adiponectin in group 2 was significantly lower than that in group 1 (P<0.05). Lower serum adiponectin and HDL-C levels (P<0.05) and higher AST level (P<0.01) were found in group 2 than those in simple fatty liver patients. In non-alcoholic steatohepatitis patients,serum adiponectin level was negatively correlated with the levels of TC, TG (r = - 0.436, P < 0.05; r = - 0.567, P < 0.01, respectively) and positively correlated with the level of HDL-C (r=0.524,P<0.01). Conclusion: The serum adiponectin level is correlated with the severity of NAFLD patients, and examination of serum adiponectin may be helpful in understanding the status of NAFLD patients.

Objective: To investigate the chemical constituents from Peucedanum decursivum. Methods: P. decursivum was extracted with 95% ethanol, the compounds were isolated via various chromatographic methods, and their structures were identified by detailed spectroscopic analyses. Results: Twelve compounds were isolated from the acetic ether fraction in the 95% extract of P. decursivum and were identified as isobergapten (1), bergapten (2), pimpinellin (3), isopimpinellin (4), columbianetin acetate (5), sphondin (6), qianhucoumarin E (7), xanthotoxin (8), osthole (9), ferulic acid (10), β-sitosterol (11), and psoralen (12). Conclusion: Compounds 1, 4, and 5 are isolated from the plants in Peucedanum L. for the first time, and compounds 2, 3, 6-10, and 12 are firstly isolated from P. decursivum.

Humans ; Hypertension ; Sympathetic Nervous System ; metabolism ; pathology

Differential diagnosis of benign and malignant ascites is of a great value in determining etiology, evaluating prognosis, and determining therapy regimens. There are many diagnostic methods for ascites, but up to now, no method can achieve high sensitivity and specificity and meet the requirements of clinical economics and feasibility at the same time. Therefore, searching for an ideal method for differential diagnosis of ascites has become an important topic of current research. This article describes the clinical value of diagnostic methods, such as molecular biological detection, cytological examination, combined determination of tumor markers, and routine tests, in the differential diagnosis of benign and malignant ascites, analyzes the diagnostic efficiency of related biomarkers, and points out that combined determination of various parameters has an increased value in the differential diagnosis of benign and malignant ascites.

Objective To observe effects of rhubarb extract on Ca dissolved quantity in demineralized enmnel. Methods Demineralization was performed after treating tooth facing with rhubarb extract of different concentrations and deionized water. The concentration of Ca was detected by ASCA biochemical analyzer after demineralization. Results Dissolved quantity of demineralized Ca in the rhubarb extract group (2 mg/ml, 4mg/ml) was significantly lower than that of the deionized water group (P<0.01) and the rhubarb extract group (1 mg/ml) (P<0.05), but higher than that of 2% sodium fluoride group (P>0.05). Dissolved quantity of demineralized Ca in the rhubarb extract (1 mg/ml) group was significantly higher thanthat of 2% sodium fluoride group (P<0.01), but lower than that of the deionized water group(P>0.05).Conclusion The rhubarb exwact (2mg/ml, 4mg/ml) can inhibit Ca dissolution in demineralized enamel.The inidal effective concentration of rhubarb extract is 2 mg/ml.

Objective To study the heart rate variability(HRV) and blood pressure variability(BPV) in patients with different grades of hypertension.Methods 120 cases of patients with hypertension,according to 1999 WHO/ISH hypertension diagnostic criteria are:1 hypertension in 40 cases,2 of hypertension in 40 cases,3 of hypertension in 40 cases and 40 cases of normal control group were detected heart rate variability and blood pressure variability.Results HRV was significantly different between the two groups(P <0.05 or p <0.01).The blood pressure increased and HRV tended to decrease.The BPV was significantly different(P < 0.01) between the two groups;the blood pressure increased and the BPV tended to increase.Conclusion The grade of hypertension is related to HRV and BPV.

Objective To investigate the satisfaction status of patients in the Community Health Service Center,and to provide the basis data for reform and development of the Community Health Service Center in future. Methods 481 patients and 9 Community Health Service Center in Shenyang were randomly recruited in this study by the cluster sampling survey.Several trained interviewers used questionnaires to collect requisite information.Results In the all community health professionals,including technical level,therapeutic efficacy,preventive health service, service attitude and requirement satiation,the trust status of patients was the highest,the rates of satisfaction and very satisfaction got to 92.8%.The satisfaction status for preventive health service was the lowest,only 67.7%.In analysis on influencing factors,medical insurance of patients was the important factor for their satisfaction status.There were significant differences in technical level (χ2 =22.61 ,P <0.01 ),therapeutic efficacy (χ2 =21 .52,P <0.01 ), preventive health service (χ2 =1 4.35,P <0.05),service attitude (χ2 =22.43,P <0.01 ),requirement satiation (χ2 =22.30,P <0.01 ),the trust status of patients (χ2 =1 7.81 ,P <0.01 ).In addition,age and education degree also were the factors on the satisfaction status of patients.There were significant differences among different age group in therapeutic efficacy (χ2 =33.38,P <0.01 ),preventive health service (χ2 =24.43,P <0.05 ),requirement satiation (χ2 =26.55,P <0.01 ),the trust status of patients (χ2 =22.39,P <0.05 ).There were significant differences among different education degree in therapeutic efficacy (χ2 =1 5.79,P <0.05),service attitude (χ2 =1 4.63,P <0.05)and the trust status of patients (χ2 =1 3.50,P <0.05).Conclusion The satisfaction status of patients for service attitude is the highest and age,education degree and medical insurance of patients are the important factors influencing satisfaction status of patients.