2.Research progress of regulation mechanism of MDMX and CK1αin p53 tumor suppressor protein
Xi WEI ; Sheng ZHANG ; Ming GAO
Tianjin Medical Journal 2015;(11):1338-1341
As a tumor suppressor, p53 is activated by numerous cellular and environmental signals, and plays a criticalrole in the cell cycle regulation, cell apoptosis and senenscence. The murine double minute (MDM)2 and double minute mu?rine 4 (MDMX) are two important regulators. MDMX is a p53 binding protein with strong sequence homology to MDM2, but lacks ubiquitin ligase activity, and which is unable to target p53 for proteasomal degradation. MDMX regulates p53 activity through its binding with p53 and its postranscriptional modification. MDMX in the closed and open structure binds to p53 to regulate its activity. As the main partner of MDMX, casein kinase 1 alpha (CK1α) disrupts the intramolecular binding in MD?MX in the cooperation to regulate p53 activity. The process of MDMX and CK1αin the regulation of p53 is multi-step and complicated. In this paper the mechanism of MDMX and CK1αin the regulation of p53 protein was reviewed.
3.Mutation analysis of the STAT3 gene in a patient with hyper-IgE syndrome
Zhongtao LI ; Sheng WANG ; Xi WANG
Chinese Journal of Dermatology 2015;48(10):735-737
Objective To analyze mutations of the STAT3 gene in a patient with hyper-IgE svudrome (HIES) mainly manifesting as multiple cold abscesses.Methods Clinical data were collected and blood samples were obtained from a 17-year-old male patient with HIES and his parents.Genomic DNA was extracted and subjected to PCR for the amplification of the entire coding region and splice sites of the STAT3 gene followed by bidirectional sequencing.Results A heterozygous missense mutation C1427T,which caused a codon change from TCC to TTC and resulted in the substitution of serine by phenylalanine at amino acid residue 476 (p.S476F), was found in exon 16 encoding the DNA-binding domain in the STAT3 gene in the patient, but not in either of his parents.The results of amplified ribosomal DNA restriction analysis were consistent with the findings mentioned above.Conclusion A novel missense mutation S426F was found in the STAT3 gene in the HIES patient with generalized cold abscesses as the prominent clinical manifestation.
4.Treatment of displaced intra-articular calcaneus fractures using the extensile lateral approach and internal fixation
Yunfei LI ; Sheng GAO ; Pingchang XI
Orthopedic Journal of China 2006;0(18):-
[Objective]To evaluate our protocol with the extensile lateral approach to open reduction and internal fixation in the treatment of displaced intra-articular calcaneus fractures.[Method]From 2001 to 2005,35 patients(41 feets)were treated with open reduction and internal fixation using extensile lateral approach,with 25 right feet and 16 left feet and with plate fixation using in 40 cases,single screw fixation using in 1 case.[Result]Evaluated with postoperative plain radiographs,90%(37/41) fracture had anatomic or near anatomic reduction of posterior facet.73%(30/41) Bhler angle above than 20? in 28 cases,32 feets among the patients were followed up at a mean of 14 months(ranged,9 to 24 mouths) and evaluated according to hind foot and ankle score system of AOFAS,average score was 87.The function were satisfactory.It took average 4 month to come back normal action,none of nerves injury,4 patients had wound hematoma.Two cases developed surgical wound edge necrosis and dehiscence among them,who were treated in initial phase in the treat group,the 2 cases were managed with dressing changes and healed in 1 month,whitout skin flaps and grafts.[Conclusion]Open reduction and internal fixation using lateral approach is a better choice to treat intra-articular calcaneal fractures,because it can correct directly the pathology of calcaneal fractures.Anatomical reduction of joint congruity and the shape of the calcaneus are important prognostic factors.
5.Force Control of Elbow and Shoulder during Isometric Contraction in Hemiparetic Stroke Patients
Chenyu ZHAO ; Sheng BI ; Xi LU
Chinese Journal of Rehabilitation Theory and Practice 2017;23(1):81-84
Objective To evaluate the ability of force control of elbow and shoulder during isometric contraction in patients with chronic stroke. Methods From January to December, 2015, 22 chronic stroke patients and 12 healthy people were measured the maximum force dur-ing shoulder abduction/adduction and elbow flexion/extension with instrument for measuring force of upper extremity. The coefficient of variation was calculated. Results The maximum force was less in the patients than in the healthy controls (t>2.349, P<0.05), and the coeffi-cient of variation increased (t>1.974, P<0.05), except those of elbow extension. Conclusion The force measure and the coefficient of varia-tion can reflect the force control in shoulder and elbow motion in stroke patients.
6.On-column Refolding of EC-SOD Inclusion Bodies, Purification and Stability Study
Xi-Qiang ZHU ; Qin-Sheng YUAN ;
Microbiology 1992;0(04):-
EC-SOD inclusion bodies was refolded on column using metal (Ni) affinity chromatography, based on the metal-binding property of His-tag. The effect of protein amount, urea removal speed and temperature on refolding was observed. We compared the different efficiency purified with Ni-sepharose and Heprain-sepharose affinity chromatography, and studied the stability of the refolded proteins. The results indicate that the inclusion bodies can be renatured with Ni-sepharose affinity chromatography. The increase of the protein amount and urea removal rate , the lower of the renaturation efficiency. Higher temperature was benefit to protein renaturation. Both the Ni-sepharose and Heprain-sepharose affinity column can be used to purified the refolded proteins, but purified by Heprain-sepharose affinity column the protein had higher activity. The activity of renatured protein was stable in 10 ℃~50℃,when pH10 its stability was lower significantly. In denaturating solution the stability of renatured protein was low.
7.Application of Mitomycin C during Operation of Pterygium for Decreasing Recurrence
yu, CHENG ; yi-sheng, ZHONG ; xi, SHEN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(11):-
0.05).The recurrence of pterygium was related to the age.If the age increased five years,the risk of recurrence decreased 18.1%. Conclusion The application of MMC(during) the operation could decrease the recurrence rate of pterygium.The recurrence rate of pterygium was not related to the time of application of 0.02% MMC,and detainment for 3 min was enough during the operation.
8.Clinical analysis on 37 cases of chronic lymphocytic thyoiditis
Wei ZHONG ; Xi SHENG ; Junyi SHI
Chinese Journal of Primary Medicine and Pharmacy 2006;0(12):-
Objective To explore the charcter and thesapeutic principlc of chronic lymphocytic thyoiditis.Methods 37 patients with chronical lymphocytic thyoiditis undergoing thyroidectomy were retrospectively analyzed.Results Compared with routine frozen section(FS) in operation FNA(fine needle aspiration) and the detecting of autoantibody had coincidence of 38%.Conclusion Exept preferred choosing of FNA and the detecting of autoantibody in early time,proper operation and the choose of indication is also important.
9. Antitumor effect of volatile oil from Sinapis Albae Semen on H22-bearing mice and its mechanism
Chinese Traditional and Herbal Drugs 2013;44(21):3024-3029
Objective: To investigate the antitumor effect of volatile oil from Sinapis Albae Semen (VOSAS) on H22-bearing mice and to determine the mechanism. Methods: To establish the H22 implanted hepatocellular carcinoma animal model which was used to analyze the effect of VOSAS on the growth of transplanted tumor. Mice were divided into five groups 24 h after modeling: model, cytoxan (CTX, 25 mg/kg) positive control, low-, mid-, and high-dose (20, 40, and 80 mg/kg) VOSAS groups. The mice were ip administered once daily for 10 d. Morphological changes in H22 solid tumor cells were observed by both Hematoxylin-eosin (HE) and acridine orange (AO) staining. The expression of Bax and Bcl-2 in the tumor tissue was determined using immunohistochemistry. Results: VOSAS could inhibit the tumor growth and extend the life span of H22-bearing mice (P < 0.01); and it could also raise the expression of Bax while suppress the expression of Bcl-2; the antitumor effect of VOSAS on H22-bearing mice demonstrated a good dose-effect relationship, but the high-dose group of the volatile oil has obvious toxicity and side effects on the mice. Conclusion: VOSAS could inhibit the growth of H22 tumor cells and the mechanism may be related to up-regulating the expression of Bax and down-regulating the expression of Bcl-2, and the induction of apoptosis.
10.Sesquiterpenoids from Solanum lyratum.
Xi-Dian YUE ; Xi-Dian YUE ; Fang YAO ; Lei ZHANG ; Gui-Sheng LI ; Sheng-Jun DAI
China Journal of Chinese Materia Medica 2014;39(3):453-456
Ten compounds were isolated and purified by column chromatography over silica gel, preparative TLC, and Sephadex LH-20 from the whole plant of Solanum lyratum. The structures were elucidated on the basis of physico-chemical properties and spectral data as 1beta-hydroxy-1 ,2-dihydro-alpha-santonin (1) , boscialin (2) , blumenol C (3), 3beta-hydroxy-5alpha, 6alpha-epoxy-7-megastigmen-9-one(4), dehydrovomifoliol(5) , blumenol A(6), (1'S,2R,5S, 10R) -2-(1', 2'-dihydroxy-l1'-methylethyl) -6,10-dimethylspiro[4,5] dec-6-en-8-one(7) , (1'R,2R,5S,10R)-2-( 1',2'-dihydroxy-l '-methylethyl) -6,1 l0-dimethylspiro[4,5]dec-6-en-8-one( 8) , 2-(1',2'-dihydroxy-1 '-methylethyl) -6,1 0-dimethyl-9-hydroxyspiro [4,5] dec-6-en-8-one (9) , and grasshopper ketone (10). Compounds 1-10 were isolated from this plant for the first time.
Drugs, Chinese Herbal
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chemistry
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Solanum
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chemistry
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Terpenes
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analysis
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isolation & purification