BACKGROUND: Preliminary study has shown that the composite materials composed of magnesium-based materials and mineralized collagen have a good supporting effect on repairing the critical defects, which can improve the mechanical strength of mineralized collagen and premature collapse during bone healing to some extent. However, magnesium-based metals degrade fast in chloride-containing solutions (including human body fluids or plasma), and the effects of releasing magnesium ions on the proliferation and differentiation of osteoblasts are unknown. OBJECTIVVE: To investigate the effects of magnesium ion combined with mineralized collagen on osteogenic differentiation of mouse preosteoblasts in vitro. METHODS: Mineralized collagen extracts were prepared from complete medium with magnesium ion concentration of 0, 5, 10, and 20 mmol/L. Mouse preosteoblasts were cultured with four mineralized collagen extracts, respectively, which were divided into mineralized collagen group, and 5, 10 and 20 mmol/L Mg2++mineralized collagen groups. The mouse preosteoblasts cultured in complete medium were used as control group. The cell morphology, proliferation, apoptosis, intracellular microfilament actin, and the activity of alkaline phosphatase and expression level of the osteogenic gene Runx2 after osteogenic differentiation were detected. RESULTS AND CONCLUSION: (1) After 24 hours of culture, the cells in the mineralized collagen group, and 5 and 10 mmol/L Mg2++ mineralized collagen groups adhered well, which showed no significant difference from the blank control group, and the elongated spindle cells with many synapses linked to the adjacent cells were observed. The cells in the 20 mmol/L Mg2++mineralized collagen group showed obvious pyknosis. (2) After 1, 3 and 5 days of culture, the cell viability in the 10 mmol/L Mg2++mineralized collagen group was significantly higher than that in the other four groups (P < 0.05). There was no significant difference among mineralized collagen, 5 mmol/L Mg2++ mineralized collagen and blank control groups (P > 0.05). The cell viability in the 20 mmol/L Mg2++mineralized collagen group was significantly lower than that in the mineralized collagen group (P < 0.05). (3) After 3 days of culture, DAPI staining showed that 20 mmol/L Mg2++mineralized collagen group had obvious nuclear disintegration, the other four groups had no obvious nuclear disintegration. (4) After 24 hours of culture, phalloidin staining showed that except the blank control and 20 mmol/L Mg2++mineralized collagen groups, the other three groups showed completely extended cell structure, and clear actin microfilaments, especially the 10 mmol/L Mg2++mineralized collagen group. (5) After 7 days of osteogenic differentiation, except for 20 mmol/L Mg2++mineralized collagen group, the activity of alkaline phosphatase and the expression level of Runx2 gene in the other three groups were significantly higher than those in the blank control group (P < 0.05), and those in the 10 mmol/L Mg2++mineralized collagen group was significantly higher than those in the 5 mmol/L Mg2++mineralized collagen and mineralized collagen groups (P < 0.05). (6) These results suggest that the combination of magnesium ion with mineralized collagen should be applied with appropriate concentration range of magnesium ion (≤ 10 mmol/L).

OBJECTIVETo study the BRCA1 mutations in patients with early-onset breast cancer and their affected relatives in Guangdong province and explore the relationship between BRCA1 mutation and the expressions of estrogen receptor(ER), progesterone receptor(PR), HER2 and ALN.

METHODSFrom 58 patients with early-onset breast cancer and their affected relatives, the genomic DNA was extracted from the peripheral blood mononuclear cells and the coding regions of the BRCA1 gene was amplified using polymerase chain reaction. BRCA1 gene mutations were screened by denaturing high performance liquid chromatography (DHPLC) and subsequent direct DNA sequencing. The expression of ER, PR, HER2 and ALN were detected with immunohistochemistry and their relations with the gene mutation were analyzed.

RESULTSDisease-related BRCA1 mutations were detected in 2 of the 58 patients, who were younger than 35 years old, including 1 with a novel splice-site mutation (IVS5-1 G-->A). No association was found between this novel mutation and the expressions of ER, PR, HER2 and ALN.

CONCLUSIONThe incidence of BRCA1 mutation is significantly lower in patients with early-onset breast cancer and their affected relatives in Guangdong province than in the Western populations. The novel mutation identified in BRCA1 gene may represent a mutation characteristic of the patients in Guangdong province. BRCA1 gene mutations may not have any relation with the expression of ER, PR, HER2 and ALN.

Adult ; Age of Onset ; Base Sequence ; Breast Neoplasms ; genetics ; China ; DNA Mutational Analysis ; Female ; Genes, BRCA1 ; Genotype ; Humans ; Molecular Sequence Data ; Mutation ; Receptor, ErbB-2 ; genetics ; Receptors, Estrogen ; genetics ; Receptors, Progesterone ; genetics

Traditional Chinese Medicine (TCM) shows unique advantages in the field of adjuvant treatment of gastric cancer. The main mechanism of TCM in improving gastric cancer includes regulating cell proliferation and apoptosis, reversing cell resistance, reducing the ability of invasion and metastasis and epithelial-mesenchymal transformation, regulating immune function, inhibiting neovascularization, regulating autophagy exosome, and ferroptosis.

AIM: To explore the effect and mechanism of circZNF124 on the proliferation, migration and invasion of colorectal cancer SW620 cells. METHODS: The expression levels of circZNF124 and miR-4262 in colorectal cancer tissues were measured by qRT-PCR method. Human colorectal cancer cells SW620 were cultured in vitro, and were randomly grouped into si-NC group, si-circZNF124 group, miR-NC group, miR-4262 group, si-circZNF124 j anti-miR-NC group, si-circZNF124 j antimiR-4262 groups. CCK-8 method, plate clone formation test, scratch test and Transwell test respectively were used to detect cell proliferation, clone formation, migration and invasion of SW620 cells. The dual luciferase reporter experiment analyzed the targeted binding of circZNF124 to miR-4262. Western blot was used to detect the expression of E-cadherin and N-cadherin protein. RESULTS: The expression of circZNF124 in colorectal cancer tissue was increased by about 3.75 times compared with that in the adjacent tissue (P i 0.05), and the expression of miR-4262 was decreased by about 0.73 times compared with the adjacent tissue (P i 0.05). Compared with the si-NC group, the cell viability, scratch healing rate and the protein level of N-cadherin in the si-circZNF124 group were decreased (P i 0.05), the number of cell clone formation and the number of invasive cells were decreased (P i 0.05), while the protein level of E-cadherin was increased (P i 0.05). circZNF124 could negatively regulate the expression of miR-4262. Compared with the miR-NC group, the cell viability, scratch healing rate and the protein level of N-cadherin in the miR-4262 group were reduced (P i 0.05), the number of cell clones and the number of invasive cells were reduced (P i 0.05), while the protein level of E-cadherin was increased (P i 0.05). Inhibition of miR-4262 expression reversed the effect of interfering circZNF124 expression on the proliferation, migration and invasion of SW620 cells.CONCLUSION: Interference with the expression of circZNF124 can attenuate the proliferation, migration and invasion of colorectal cancer cells by targeting miR-4262.