Comparative Genomic Hybridization ; methods ; DNA ; Gene Expression Profiling ; methods ; Genetics, Medical ; methods ; Humans ; In Situ Hybridization, Fluorescence ; Oligonucleotide Array Sequence Analysis ; methods

Objective To investigate radionuclide imaging pattern with ~ 99m Tc-MDP in rat arthritis induced by collagen, and the significance of the imaging in the early diagnosis of arthritis. Methods 70 male Wistar rats were divided into normal (n=28) and model group (n=42), both groups were respectively subdivided into 7 groups according to time of treatment. Collagen induced arthritis (CIA) model was reproduced by subcutaneous injection of collagen and adjuvant. For imaging, rats were injected with ~ 99m Tc-MDP through tail vein. The technique of Radome of Interesting (ROI) was used in knee and whole bone, and R value was calculated. The concentration of radionuclide was observed and compared with arthritis index (AI), paw thickness, pathological changes and ?-ray examination. Results Rats in model group showed higher concentration of radionuclide in the knee joint (P

Objective To evaluate the role of differentially expressed genes in human prostate carcinoma initiation and progression. Methods cDNA microarray chips which consist of a set of 4366 human genes were used to investigate the gene expression pattern of samples from human prostate cancer and normal prostate tissues. Results Of 4366 genes,287 genes differentially expressed in prostate carcinoma and normal prostate tissues were screened out,including 165 unknown genes and 122 known genes. Among the 122 known genes we further identified 20 up-regulated and 36 down-regulated genes. Conclusions Our results suggest that significantly differential expression of genes may be associated with the pathogenesis and progression of the prostate cancer.

Objective To evaluate the effect of smecta on acute gastroenteritis induced by the radiotherapy in human cervical cancer.Methods seventy-eight patients under radiotherapy received smecta combined with general medicine to control gastroenteritis. All patients were divided into 2 groups:group A: 40 patients were treated with smecta and general medicine.group B:38 patients were only routinely treated. The routine medicine included prednisone, vitamin B 2 and ciprofloxacin.Results The effective rate for acute gastroenteritis in A group was significant higher than in B group ( P

Objective To investigate the clinical factors and therapeutic tactic for rebleeding of hypertensive hemorrhage after minimally invasive craniopuncture.Methods Clinical data of 213 cases were reviewed retrospectively.8 possible factors were gathered to select the high-risk ones by multiple-factor Logistic regression analysis.Results 27 cases bled after minimally invasive craniopuncture therapy.By Logistic regression analysis,we found 4 high-risk factors of recurrence hemorrhage,including hematoma shape (β =2.236,P =0.002),systolic blood pressure on admission (β =1.877,P =0.001),operation time (β =-1.589,P =0.004) and hematoma clearance (β =1.280,P =0.010).Conclusion Paying more attention to the 4 factors and treating each patient by individual therapeutic tactic according to the 4 factors will help to decrease the incidence of bleeding after minimally invasive craniopuncture.

Scientific research to a great extent,represents the comprehensive strength of a unit,directly determine the development prospects of this unit.At present,our country many medical institutions of scientific research strength is low,potential is insufficient,can't meet the development of medical institutions.This study from the aspects of management performance,to evaluate scientific research ability from various perspectives,statistical analysis of its influencing factors at the same time,Reflects the technical level of this unit,discipline construction,scientific research and development and performance,produced by scientific management for the unit provides an objective basis for scientific research management departments to formulate relevant policies to explore to improve the new management starting point,thus achieve the goal of science and technology.

Objective To evaluate the relationship between annexin 1 (ANXA1) and the endogenous protective mechanism during intestinal epithelial cell injury induced by endotoxiu.Methods The intestinal epithelial cells at the logarithmic growth phase were seeded in culture palates and randomly divided into 4 groups (n =36 each) using a random number table:control group (group C),cell injury group (group I),ANXA1 overexpression group (group OE),and ANXA1 silencing group (group S).Lentivirus with ANXA1 overexpression and silencing was transfected into intestinal epithelial cells to construct a stable cell line.In I,OE and S groups,endotoxin was added with the final concentration of 100 μg/ml,and the cells were then incubated for 24 h to establish the cell injury model.The culture medium was changed,and the cells were then incubated for 24 h in group C.The cell apoptosis was detected by flow cytometry,the cell permeability was determined by Transwell assay,and the cell viability was evaluated by methyl thiazolyl tetrazolium assay.The apoptosis rate was calculated.Results Compared with group C,the apoptosis rate was significantly increased,and the cell permeability and viability were significantly decreased in I,OE and S groups (P＜0.05).Compared with group Ⅰ,the apoptosis rate was significantly decreased,the cell permeability and viability were significantly increased in group OE,and the apoptosis rate was significantly increased,and the cell permeability and viability were significantly decreased in group S (P＜0.05).Conclusion ANXA1 is involved in the endogenous protective mechanism during intestinal epithelial cell injury induced by endotoxin.

Aim To investigate the effect of ASX (trans-astaxanthin)on the expressions of NF-κB p65 , iNOS and TNF-αin the hippocampus and the prefron-tal cortex of chronic alcohol mice.Methods 40 mice were randomly divided into control group,7 d,14 d, 21 d,28 d alcohol-treated group,the mice were given alcohol preference testing on day of 6,13,20,27. Mice were subjected to alcohol withdrawal for one day after testing.In order to determine the exact time point of cognitive memory impairment in mice after alcohol consumption,they were given morris water maze test after alcohol preference testing. The other 40 mice were randomly divided into control group, alcohol group and ASX group (20,40,80 mg·kg-1 ).After chronic ASX administration, mice were given one probe trial of 60 s in which the platform was removed from the pool to evaluate escape latency,the number of times the animal crossed the previous location of the platform,time spent in the target quadrant,and swim-ming speed.The expressions of NF-κB p65 ,iNOS and TNF-αwere detected by western blotting after behav-ioral testing.Results The mice showed an obvious al-cohol-related phenomenon on 2 1 and 28 days after al-cohol treatment,and escape latency significantly in-creased,entries in target quadrant and duration in tar-get quadrant significantly decreased with increasing drinking days and withdrawal times.The results also suggested that 2 1 days chronic ASX treatment reversed this learning deficit.Moreover,the expression of NF-κB p65 ,iNOS and TNF-αin the hippocampus were significantly increased after 2 1 d alcohol treatment (P<0.001),and pretreatment with ASX (40,80 mg· kg-1 ) could obviously inhibit these changes (P <0.001);Parallel to these changes in the hippocam-pus,the level of NF-κB p65 ,iNOS and TNF-αwere also increased in the prefrontal cortex (P<0.001 ), however,only ASX (80 mg · kg-1 ) administration could inhibit the increase (P<0.05 ).Conclusion These results indicate that ASX pretreatment can pro-tect against alcohol-induced memory impairment via the inhibition of NF- κB p65 ,iNOS and TNF-αexpres-sions in hippocampus and prefrontal cortex.

AIM: To determine whether interleukin 4 and interleukin 4 receptor ? chain are associated with allergic asthma in children and to study the impact of such polymorphism upon plasma IgE.METHODS: Two polymorphism sites of IL-4 and IL-4R were studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).RESULTS: (1)The results showed that the IL-4 promoter-589 was not associated with children allergic asthma, however, the IL-4R ? chain 576RR genotype and R allele were significantly increased in the subjects with asthma in children compared with age-matched control subjects (?2=11.84, P

Objective To observe the effects of Huoxue Chubi Decoction on contents of PDGF-A and TGF-βin skin lesion of scleroderma mimicking mice models;To discuss its possible mechanism. Methods Thirty mice were randomly assigned into blank group, model group, TCM high-, medium-, low-dose group, with six mice in each group. Other than blank group, the rest of the 4 groups were given bleomycin-hydrocholoride 0.1 mL intradermal injection daily for 3 weeks to establish scleroderma mice models. From week 4 to 7, blank group and model group received gavage with equal amount of distilled water daily;TCM high-, medium-, low dosage group received gavage with Huoxue Chubi Decoction with different concentrations of 44.8, 22.4, 11.2 g/kg daily. The pathological changes in the skin tissue samples taken from each group were observed under microscope;the thickness of skin from each group was measured and the expression of PDGF-A, TGF-β, type-Ⅰ collagen (COL-Ⅰ) and type-Ⅲ collagen (COL-Ⅲ) were tested through immunohistochemical staining. Results Compare with blank group, the dermis tissue of model group was thicker, with the presence of thicker and greater number of collagen fibers, as well as infiltration of inflammatory cells. Compare with model group, thickness of skin and dermis collagen growth of TCM high-, medium-, low-dose group were milder varied by the concentration of the decoction. The result of immunohistochemical staining showed that the expressions of PDGF-A, TGF-β, COL-Ⅰ and COL-Ⅲ of modelgroup significantly increased than blank group (P<0.01); the expression of the mentioned indicators were statistically significant lower in the TCM high-, medium-, low-dose group than model group; The expressions of PDGF-A and TGF-β showed a positive correlation with the amount of COL-Ⅰ and COL-Ⅲpresented in each group. Conclusion Huoxue Chubi Decoction can suppress collagen expression and fibroblast formation in skin lesion of scleroderma mice models by down regulating the expressions of TGF-β and PDGF-A, thus demonstrate its potential in scleroderma treatment.