Background Auto-fluorescence(AF)imaging is a new non-invasive fundus examining technology.It is now playing an increasingly important role in the diagnosis of retinal diseases.Using a confocal Scanning Laser Ophthalmoscope microscope(cSLO),two types of AF can be obtained:melanin-related near-infrared fundus auto-fluorescence(NIA)and lipofuscin-related fundus auto-fluorescence(FAF).Objective The purpose of this study was to compare the outcome between NIA and FAF in patients with central serous chorioretinopathy (CSC).Methods FAF,NIA imaging and fundus fluorescein angiography(FFA)were performed on 28 eyes of 23 subjects using a cLSO.AF images were evaluated and compared with angiographic findings.Written informed consent was obtained before the initiation of this study.Results In 17 eyes of 15 cases with acute CSC,the leakage points on the FFA image showed three kinds of AF patterns:(1)hyper-AF,including FAF hyper-AF in 2 eyes(11.76％)and NIA hyper-AF in 4 eyes(23.53％).(2)Non-AF,including FAF non-AF in 10 eyes(58.82％)and NIA non-AF in 13 eyes(76.47％).(3)Normal AF,including normal AF of FAF in 5 eyes(29.42％).The AF in the retinal serous detachment area showed two kinds of characteristics:(1)hypo-AF,including 12 eyes(70.59％)with FAF hypo-AF and 10 eyes(58.82％)with NIA hypo-AF.(2)hyper-AF,including 5 eyes(29.41％)with FAF hyper-AF and 7 eyes (41.18％)with NIA hyper-AF.In 11 eyes of 8 cases with chronic CSC,the leakage on the FFA also showed an absence of AF.However,AF-absent areas also partly appeared in the non-leakage area of FFA.In NIA,the non-AF areas were often more visible than that in FAF.Serous detachment areas in chronic CSC usually displayed multiple AF forms,including granular-like non-AF,hyper-AF and hypo-AF,and the abnormal AF areas were often greater than that of the corresponding abnormal fluorescent area in FFA.Conclusions AF can be used to detect the functional status and metabolic activity of RPE in CSC eyes.

Diabetic cardiomyopathy(DCM) is a type of primary cardiomyopathy caused by diabetes, changing in the structure and function of the myocardium, which is distinguished from secondaiy cardiomyopathy resulted from coronary atherosclerosis, hypertension, and viruses. Phosphatidylinositol 3-kinase (PI3K)/Akt is an important signal transduction pathway in human body involved in the growth and reproduction of body. Regulatory factors of the PDK/Akt signaling pathway include silencing information regulator 1, homologous phosphatase-tensin, glycogen synthase kinase 3(3 and the rapamycin target protein. The role of PDK/Akt signaling pathway in the pathogenesis of DCM includes regulating blood sugar level, regulating lipid metabolism, protecting endothelial cells, reducing inflammatory response , improving cardiac function, resisting fibrosis, and regulating myocardial apoptosis. The development of PI3K-Akt signaling pathway inhibitors provides the possibility for PDK-Akt pathway targeted therapy. This article reviews the progression of the PDK/Akt pathway in DCM.

Objective To observe the effect of treadmill training on metabotropic glutamate receptor of a middle cerebral artery occlusion model in rat and its underlying mechanisms. Methods Sprague-Dawley male rats of 2 or 3 months old were randomly divided into 3 groups: a sham operation group, an iscbemia-reperfnsion group and a treadmill training group (subject to 2 weeks of training after the ischemia-reperfusion). All the animals were sacri-ficed after 2 weeks of training and their brains were sampled for measurement of the expression level of the striatum mGluR Ⅰ-mRNA, using RT-PCR technique. Results The striatum mRNA of mGluR1 and mGluR5 was signifi-cantly elevated in the ischemia-reperfusion group(P<0.01), but treadmill training significantly suppressed the ele-vation of the expression of mRNA of mGluR1 and mGluR5 (P<0.01). Conclusions Treadmill training can signif-icantly downregulate mGluR Ⅰ-mRNA expression. This might be one of the important mechanisms for inhibition of the excitatory glutamate production.

Adult ; Female ; Hexanes ; poisoning ; Humans ; Immunity, Humoral ; Male ; Myelin Proteins ; blood ; immunology ; Peripheral Nervous System Diseases ; chemically induced ; immunology ; Young Adult

[Objective]To investigate the treatment and result of the meniscal cyst treatment by arthroscopic partial meniscectomy and decompression of the cyst.[Method]Nine patients who had suffered from meniscal cysts were treated by arthroscopic partial meniscectomy and decompression of the cyst in General Hospital of Yanzhou Coal Mine Group during the period from January 2002 to December 2005.There were 3 males and 6 females.The age ranged from 16 years to 54 years,with an average of(32.5?14.1) years.The duration was between 2 months and 2 years with an average of(13.4?6.8) months.One patient had medial meniscal cyst and eight patients had lateral meniscal cysts.There were 5 cysts in meniscal anterior horn,3 cysts in meniscal body and 1 cyst in meniscal posterior horn.Meniscal lesions adhering to cysts included 6 horizontal cleavages and 3 complex cleavages.Arthroscopy was performed to identify the hole through the meniscus to cyst,then the meniscal tear was partially resected taking great care to preserve the meniscal rim.The planer was used to decompress the cyst and excise the wall of the cyst and broaden the passage between the cyst and the main knee compartment.After operation,all patients were instructed to perform quadriceps exercises.The patients were followed up from 6 to 38 months with an average of(17.2?9.8) months.A conclusion was deduced from the pre-and post-operative Lysholm score of the patients.[Result]None of the 9 patients reoccored during follow-up.After operation,the symptoms of the knee pain and swelling disappeared,the range of the knee motion restored,poping and locking didn't reoccur,the fullness at the site of the cyst died away.The complication of the operation didn't occur such as infection,damage of the vascular and nerve and restriction of the knee motion.Lysholm score of the 9 patients showed a significant improvement from(70.2?7.8) pre-operation to(96.7?3.3) post-operation(t=9.3868,P=0.000).[Conclusion]Arthroscopic partial meniscectomy and cyst decompression has obvious advantages of preserving the function of the meniscus and decreasing the incidence of the knee osteoarthritis.It should be the primary treatment of the meniscal cyst owing to its low morbidity and satisfying results.

To analyze the change of physiological indices in male and female pilot students during lower body negative pressure (LBNP) exposure, one female group and one male group participated in the experiment. They were subjected to LBNP up to 50 mmHg for 8 min. Electrocardiogram (ECG), heart rate (HR), blood pressure (BP), cerebral blood flow (CBF) and time of exposure were monitored during LBNP. The results showed that during LBNP, heart rate (HR) increased while systolic blood pressure (SBP) and cerebral blood flow (CBF) decreased with the prolongation of exposure. It is concluded that LBNP exposure could produce similar physiological changes in both male and femal pilot students.

Objective To investigate the expressions of glucagon-like peptide-1 receptors (GLP-1R) and cytokeratin17 (CK17) in papillary thyroid cancer tissue and clinicopathological significance.Methods The immunohistochemistry and immunoblotting method were employed to detect the expressions of GLP-1R and CK17 in normal thyroid tissues (control group,50 cases) and papillary thyroid cancer tissue (observation group,80 cases).Then the correlation between the expressions of GLP-1R and CK17 with pathological factors of thyroid cancer was analyzed.Results Low expression of GLP-1R was found in 22 cases (44.00 ％) of the control group,while GLP-1R was expressed in 77 cases(96.25 ％) of the observation group,and the difference between both groups was statistically significant(P＜0.01).The expression of CK17 was positive in 8 cases(16.00％) of the control group,while 77 cases(96.25 ％) in the observation group were CK17 expression positive,and the difference between both groups was statistically significant(P＜0.01).The difference between GLP-1 positive expression and clinicopathologic factors had no statistical significance(P＞0.05).The difference between CK17 expression with sex and age had no statistical significance (P＞0.05),and was obviously related with the tumor diameter,clinical stages and cervical node metastasis of thyroid neoplasms(P＜0.05).Conclusion GLP-1R and CK17 all are highly expressed in papillary thyroid cancer tissue and CK17 expression is obviously related with the tumor diameter,clinical stages and cervical node metastasis of papillary thyroid cancer.

Background The early diagnosis and treatment is vital for the age-related macular degeneration (AMD).Previous examining methods were fundus fluorescein angiography(FFA)and optical coherence tomography (OCT).However,a new diagnostic technique,fundus autofluorescence(FAF),is applying in ophthalmology.objective This study was to observe the FAF characteristics of atrophic AMD. Methods Thirty-nine eyes of 28 patients with atrophic AMD were diagnosed and collected in our hospital and classified based on the criteria of Chen Song.FAF was performed by a confocal scanning laser ophthalmoscope(Heidelberg Retina Angiograph 2,HRA2).Characteristics of FAF images were compared with FFA images or color fundus photographs.Written informed consent was obtained from each patient prior to the examination. Results Areas with abnormal increased or decreased FAF signals were consisted or unconsisted with funduscopically visible alterations.Seven manifestations of FAF were found in the eyes with preatrophic stage of atrophic AMD,including slight change,focal increase pattern,patchy pattern,linear pattern,lacelike pattern,reticular pattern,and speckled pattern.In atrophic stage of atrophic AMD,geographic atrophy showed a well-defined hypoautofluorescence area;while non-geographic atrophy showed a ill-defined hypoautofluorescence area.There were three abnormal FAF patterns(focal,band and diffuse)in the junctional zone of geographic atrophy and diffuse pattern in the junctional zone of non-geographic atrophy. Conclusion FAF imaging is helpful for the diagnosis of atrophic AMD as a new non-invasive technique.

AIM: To evaluate the use of 23G minimally invasive vitreous cutting system in the first stage of anterior segment reconstruction on severe ocular injury.
METHODS: Fifteen patients with ocular injuries including the corneal and scleral rupture associated hyphema, vitreous hernia in anterior chamber, traumatic lens rupture, lens subluxation, applied 23G minimally invasive vitreous cutting system in the first stage of anterior segment reconstruction.
RESULTS: The anterior ocular media of early postoperative became transparent quickly. No exudative lemma and no complications were found relate to puncture incisions after operations.
CONCLUSION:23G minimally invasive vitreous cutting system can be used in the first stage of anterior segment reconstruction on sever ocular injury. It can reduce iatrogenic injury, operative complications and inflammatory response, also can shorten therapeutic time and create favorable conditions.

ObjectiveTo investigate the expression and the clinical significance of diacylglycerol kinase α (DGKα) and protein kinase C (PKC) in human hepatocellular carcinoma (HCC).MethodsDGKα and PKC expressions in the samples from 60 pathologically confirmed HCC patients were analyzed by immunohistochemistry. The relationship between DGKo expression and clinical pathology factors was analyzed.ResultsThe expression positive rates of DGKo and PKC were highest in normal liver tissues [90.0％ (9/10) and 100.0％ (10/10)].The positive rates were 81.7 ％ (49/60) and 71.7 ％ (43/60) in HCC tissues,respectively,and were 58.3 ％ (35/60) and 61.7 ％ (37/60) in carcinoma adjacent tissues,respectively.In three liver tissues,the positive rates of DGKα and PKC were significantly different (P ＜0.05).The location of both kinases in hepatocytes translocated from cytoplasm/nucleus to membrane.The expressions of DGKα and PKC were positively correlation(r =0.495, P ＜ 0.05), The DGKα expression was correlated to differentiation type,portal venous tumor thrombus and TNM staging(all P ＜ 0.05).ConclusionDGKa is expressed with high activity in advanced stage and poorly differentiated HCC. It may be promote the pathological process of HCC.