Rehabilitation medicine is a comprehensive multi-discipline. It is important to use varied Methods , modern and traditional, rationally in teaching rehabilitation medicine to improve the quality and efficiency. Some of these Methods were discussed.

Objective: To construct the prokaryotic expression vector bearing fusion gene NT4-ADNF-9 and lay foundation for further study on genetic therapy of neurosensory deafness. Methods: By means of asymmetrical primer/ template, double stranded cDNA of activity dependent neurotrophic factor-9 (ADNF-9) was obtained, which included restriction enzymes sites on the two extremities. ADNF-9 cDNA was ligated to the signal and leader peptides of neurotrophin 4 (NT4), and the fusion gene was named NT4-ADNF-9. Then it was subcloned into prokaryotic expression vector pBV220, and called pBV220/ NT4-ADNF-9. Results: Evidences of DNA sequence analysis and restriction enzymes digestion showed that we recombined ADNF-9 cDNA to the 3′terminal of the signal and leader peptides of NT4, and the fusion gene was subcloned into pBV220 successfully. Bioactivity of the products was proved that it could support the cell survival and neurite growth in the primary cultures of dorsal root ganglia (DRG) of embryonic day-8 chicken neurons as compared to the control. Conclusion: Prokaryotic expression vector pBV220/NT4-ADNF-9 can be constructed successfully and the bioactivity is satisfactory.

Objective: To construct the prokaryotic expression vector bearing fusion gene NT4-ADNF-9 and lay foundation for further study on genetic therapy of neurosensory deafness. Methods: By means of asymmetrical primer/ template, double stranded cDNA of activity dependent neurotrophic factor-9 (ADNF-9) was obtained, which included restriction enzymes sites on the two extremities. ADNF-9 cDNA was ligated to the signal and leader peptides of neurotrophin 4 (NT4), and the fusion gene was named NT4-ADNF-9. Then it was subcloned into prokaryotic expression vector pBV220, and called pBV220/ NT4-ADNF-9. Results: Evidences of DNA sequence analysis and restriction enzymes digestion showed that we recombined ADNF-9 cDNA to the 3′terminal of the signal and leader peptides of NT4, and the fusion gene was subcloned into pBV220 successfully. Bioactivity of the products was proved that it could support the cell survival and neurite growth in the primary cultures of dorsal root ganglia (DRG) of embryonic day-8 chicken neurons as compared to the control. Conclusion: Prokaryotic expression vector pBV220/NT4-ADNF-9 can be constructed successfully and the bioactivity is satisfactory.

Exposure to traffic-related air pollutants(TRAP)has been implicated in airway allergic diseases.Recent findings include epidemiologic and mechanistic studies that shed new light on the impact of TRAP on allergic rhinitis(AR)and the biology underlying this impact.These studies have found that oxidative stress induced by TRAP could affect the axis of epithelial cell-dendritic cell-T cell towards a T-helper 2 immune response,which is the major mechanism between TRAP and AR.Further,epigenetics and microRNA might be involved in this process.Our review will summarize the most recent findings in each of these areas.
Air Pollutants ; toxicity ; Air Pollution ; Humans ; Oxidative Stress ; Rhinitis, Allergic ; etiology ; Vehicle Emissions ; toxicity

Objective To find the optimal stimulation parameters of electroacupuncture for incision pain and visceral dragging pain after surgery.Method Databases including the China National Knowledge Infrastructure (CNKI), WanFang (WF), VIP and PubMed were retrieved by using computer. The eligible literatures related to the clinical and experimental studies on electroacupuncture for post-operative pain were summarized and the frequencies of the involved electroacupuncture parameters were counted.Result For incision pain, electroacupuncture was used after surgery, with continuous wave, usually a low frequency ranged 2~15 Hz and a current of 2~5 mA; for visceral dragging pain, electroacupuncture was used before surgery, with sparse-intense wave, alternate frequencies majorly at 2/100 Hz and a medium or low current intensity within patient’s endurance. Conclusion In clinical application, the elctroacupuncture parameters should be chosen carefully according to the type of post-operative pain.

Objective To investigate the relationship between carotid plaque and serum C reactive protein(CRP) levels, leukocyte count in patients with acute cerebral infarction(ACI) Methods Carotid duplex examination was performed in 121 patients with ACI by an Advanced Technology Laboratories HDI (high definition imaging) 5000 triplex system Serum CRP was measured by nephelometry Results of carotid ultrasonography were divided into two groups: M1: normal (IMT 0 05) The number of patient with elevated CRP levels was increased in the M2 group( P

Objective To study cell proliferation and apoptosis of renal corpuscle in normal developing mouse.Methods PCNA immunohistochemisty method,terminal deoxynucleotidyl transferase mediated dUTP-biotin nick endlabeling(TUNEL staining method)and light,electron microscopy were used to observe proliferation and apoptosis within corpuscle at different stages of development.Results Nearly all cells in Ⅰ and Ⅱ stages had nuclear PCNA immunolabeling.The number of PCNA immunolabeling cells during Ⅲ and Ⅳ stages was gradually decreased,and to less in Ⅴ stage.TUNEL positive cells could be easily observed before birth and identified in Ⅲ and Ⅳ stages.Electron microscope revealed that the mitotic activity was observed in all cells of Ⅰ and Ⅱ stages renal corpuscles.The mitotic phases of endothelial cells and podocytes could be seen in Ⅲ stage,but only endothelial mitotic activities in Ⅳ stage of renal corpuscle.Apoptotic activities of endothelial cell and Bowman's parietal cell were noted,predominantly in Ⅲ and Ⅳ stages.Conclusion Proliferation and apoptosis exist universally in the course of developing renal corpuscle,and supervise and control the regular development of renal corpuscle cooperatively.

A catheter(0.3 mm O.D.)was introduced into the external carotid artery(ECA) lumen into the lumen of the internal carotid artery(ICA), then it was gently advanced to the intracranial segment of ICA until the intraluminal catheter was 2~3mm from the origin of MCA. At this point thrombin were injected into ICA to induce thrombosis. Behavior disorders were observed at 3, 6, 12h after the operation. Willis circle, infarct volume and histopathologic changes were examined 12 hours after operation. The results showed that the model had stable behavior disorder score and infarct volume. Mixed thrombus could be observed at the origin of the MCA. Infarction volume of this model was similar with that of the model developed by linear occlusion of MCA in our laboratory. The model has the advantages of easy operation, reliable ischemia and reproducible infarct volume. It may be useful in documenting the safety and efficacy of thrombolytic therapies.

0.05). In stratified studies, risk of asthma in individuals with null genotype of GSTM1 is 2.667 times of that with wild genotype after exposure to light air pollution. Risk of asthma in individuals living in heavy air pollution area is 2.125 time of that in light pollution area for all wild genotype of GSTM1 individuals, but without statistical significance. Conclusion It was not found that the relationship between GSTM1, GSTT1 polymorphism and asthma. Synergism of genotype of GSTM1, GSTT1 and air pollution was not also seen in this study.

Aim To explore the effect of matrine( Mat) on the apoptosis of ovarian cancer SKOV3 cells and its related mechanism. Methods SKOV3 cells were treated with different concentrations( 0. 4,0. 8,1. 2,1. 6, 2. 0 g?L -1) of Mat for 24,48,72 h. The inhibitory rate of mat on SKOV3 cell proliferation was determined by MTT assay,and cell apoptosis was evaluated by flow cytometry ( FCM) ; the expression of survivin mRNA was measured by RT-PCR and the expression of survivin and Caspase-3 protein was measured by Western blot. Results Different concentrations of Mat had certain inhibitory effect on SKOV3 cell proliferation,in a dose-and tine-dependent manner. The half maximal inhibitory concentration ( IC50 ) of 24,48,72 h were 3. 38,1. 57,1. 13 g?L -1 respectively; the cell apoptotic rate of treated with 0. 8,1. 6 g?L -1 Mat for 48 h, was( 11. 44 ? 0. 56) % ,( 17. 68 ? 0. 98) % respective-ly,which had statistical significance compared with the control group( 4. 85 ? 0. 31) % ( P