1.Effects of oxymatrine on the expressions of pro-collagen and fibronectin of fibroblasts derived from human hyperplastic scars.
Xi BIAN ; Jiang-qun WU ; Xing-ju NIE
Chinese Journal of Integrated Traditional and Western Medicine 2012;32(10):1390-1393
OBJECTIVETo study the effects of oxymatrine (OM) on the expressions of pro-collagen I (PC I), pro-collagen II (PC III), fibronectin (FN), matrix metalloproteinase-1 (MMP-1) mRNA of fibroblasts from keloid (KFb), hyperplastic scar (HFb), and normal skin (NFb), and to compare with hydrocortisone (HC).
METHODSThe primary KFb, HFb and NFb were derived from patients and cultured in vitro using tissue block culture method. The fibroblasts were treated with 500 microg/mL OM, 2 microg/mL HC, or without any medicine (as the control). The mRNA expressions of PC I, PC III, FN, MMP-1 of the fibroblasts were detected using RT-PCR.
RESULTSUnder the normal condition, when compared with NFb, the mRNA expressions of PC I of KFb and HFb increased by 31.7% and 34.2% (both P < 0.05). Besides, the mRNA expression of PC III of KFb increased by 44.9% (P < 0.01). OM down-regulated the mRNA expressions of FN and PC I of HFb by 18.8% and 23.6% respectively (both P < 0.05). HC decreased the mRNA expressions of FN and PC I of HFb by 26.8% and 43.6% respectively (P < 0.05, P < 0.01). Meantime, OM up-regulated the mRNA expression of MMP-1 of KFb by 21.8% (P < 0.05).
CONCLUSIONSOM suppressed the synthesis of extracellular matrix (ECM) possibly through down-regulating the mRNA expressions of PC I and FN. Compared with HC, OM could promote the degradation of ECM through inducing the MMP-1 mRNA expressions of KFb. Therefore, OM could be potentially used in treatment of hypertrophic scar and keloid.
Alkaloids ; pharmacology ; Cells, Cultured ; Cicatrix ; metabolism ; pathology ; Extracellular Matrix ; metabolism ; pathology ; Fibroblasts ; drug effects ; metabolism ; pathology ; Fibronectins ; metabolism ; Humans ; Keloid ; metabolism ; pathology ; Matrix Metalloproteinase 1 ; metabolism ; Procollagen ; metabolism ; Quinolizines ; pharmacology
2.Lead acetate induced DNA damage in blood lymphocytes of rats.
Jian-hua ZHOU ; Lian XUE ; Xi-jin SHI ; Liu-ming PENG ; Chen BIAN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(5):290-292
Animals
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Comet Assay
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DNA Damage
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drug effects
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Lymphocytes
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drug effects
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Male
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Organometallic Compounds
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toxicity
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Rats
3.Effects of Ginsenoside Re on MDA Content and SOD Activity in Rats with Exercise-induced Fatigue
Yichong FENG ; Ziming ZHAO ; Yuan CHEN ; Huashan PAN ; Jiahong LI ; Bogao BIAN ; Xi WEN
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(06):-
Objective This report describes the mechanism of Ginsenoside Re to resist Exercise-induced fatigue based on the observation of effects on MDA content and SOD activity.Methods Thirty male SD rats were divided into 3 groups randomly:Ginsenoside Re group,model group and control group,10 rats in each group.Rats in Ginsenoside Re group were given gastric gavage of Ginsenoside Re once a day,while rats in model and control groups were given the same volume of normal saline.One hour after administration,rats of Ginsenoside Re groups and Model groups received medium-intensity treadmill exercise for 20 minutes at the speed of 15m/min,with a slope of 0 degree,and after 40-mimute break repeated it for another 20minutes.Fourteen days later,MDA content and SOD activity have been tested.Results MDA content in the serum,liver tissue and muscle tissue of Ginsenoside Re group was obviously lower than that in the model group (P
4.Total flavonoid from Glycyrrhizae Radix et Rhizoma and its ingredient isoliquiritigenin regulation M2 phenotype polarization of macrophages.
Yuan-lai WANG ; Xi TAN ; Xiao-lu YANG ; Xiao-yun LI ; Ka BIAN ; Dan-dan ZHANG
China Journal of Chinese Materia Medica 2015;40(22):4475-4481
To study the mechanisms of total flavonoid from Glycyrrhizae Radix et Rhizoma (TFGR) and its ingredient isoliquiritigenin (ISL) on their regulation of M2 phenotype polarization of macrophages. IL-4 (60 μg x L(-1)) induced RAW264.7 cells for 6 h to establish the M2 macrophage model. TFGR and ISL restrained breast cancer cells migration with the aid of M2 macrophages in vitro. TFGR and ISL inhibited gene and protein expression of Arg-1, up-regulated gene of HO-1 and protein expression of iNOS, enhanced the expression of microRNA 155 and its target gene SHIP1, meanwhile down-regulated.the phosphorylation of STAT3 and STAT6. So TFGR and ISL were the bioactive fraction and ingredient in Glycyrrhizae Radix et Rhizoma to reverse M2 phenotype macrophages polarization. TFGR and ISL inhibited the promotion of M2 macrophages to breast cancer cells migration in vitro, STAT signal pathways and miR155 were partly involved.
Animals
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Cell Line, Tumor
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Cell Movement
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drug effects
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Cell Polarity
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drug effects
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Chalcones
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pharmacology
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Flavonoids
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pharmacology
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Glycyrrhiza
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chemistry
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Interleukin-4
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genetics
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metabolism
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Macrophages
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cytology
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drug effects
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metabolism
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Mice
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RAW 264.7 Cells
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Rhizome
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chemistry
5.Metabolomic study on preventive effect of Aqueous extract and Volatile oil of Dalbergia Odorifera on myocardial ischemia/reperfusion injury in rats
Fei MU ; Jialin DUAN ; Haixu BIAN ; Rui LIN ; Peijin SHANG ; Zhihui ZHU ; Aidong WEN ; Miaomiao XI
Chinese Pharmacological Bulletin 2016;32(10):1377-1382
Aim To investigate the changes of serum metabolism after the treatment of DOA and DOO on myocardial ischemia reperfusion ( MI/R ) injury in rats, and to explore the pathogenesis of MI/R injury and drug action mechanism. Method The serum samples of Sham group, Model group, DOA group and DOO group of rats were acquired, gas phase time of flight mass spectrometry ( GC-TOF-MS) was applied to analyze the metabolic profiles of the samples. After da-ta preprocessing, they were processed into SIMCA 14. 1 software for multivariate statistical analysis. Results By principal components analysis ( PCA ) , partial least squares analysis ( PLS-DA) and orthogonal partial least squares analysis ( OPLS-DA ) , the Model group and Sham group were obviously separated, the drug in-tervention group and Model group were separated and close to Sham group. The therapeutic effect of DOO and DOA on MI/R injury in rats was proved. The ex-perimental results identified 13 endogenous biomark-ers, which were related to the glucose metabolism,lipid metabolism and amino acid metabolism pathway. Con-clusion DOA and DOO may protect the MI/R injured rats by regulating the glucose metabolism, lipid metab-olism and amino acid metabolism pathway.
6. Epidemiological investigation of hospitalized children with burn injury in a hospital of Qingdao
Xi BIAN ; Zhijun ZHU ; Ye WANG
Chinese Journal of Burns 2017;33(7):415-418
Objective:
To investigate epidemiological characteristics of hospitalized children with burn injury in the author′s affiliation, so as to provide theoretical basis for developing prevention strategies of children with burn injury.
Methods:
Medical records of 384 and 596 hospitalized children with burn injury, aged 0 to 12-year-old, were collected respectively from January 2001 to December 2005 and January 2011 to December 2015. Percentage of children with burn injury to total hospitalized patients with burn injury in the same period of time, age, causes of injury, gender, injury month, residence, condition of first aid measures conforming to medical standard, time of admission post injury, burn degree, and operation condition of children with burn injury were analyzed. Data were processed with Mann-Whitney
7.Calcium/calmodulin-dependent protein kinase II is involved in the transmission and regulation of nociception in naïve and morphine-tolerant rat nucleus accumbens
Kai Wen XI ; De Duo CHEN ; Xin GENG ; Yan BIAN ; Min Xin WANG ; Hui BIAN
The Korean Journal of Pain 2023;36(2):163-172
Background:
Synaptic plasticity contributes to nociceptive signal transmission and modulation, with calcium/ calmodulin-dependent protein kinase II (CaMK II) playing a fundamental role in neural plasticity. This research was conducted to investigate the role of CaMK II in the transmission and regulation of nociceptive information within the nucleus accumbens (NAc) of naïve and morphine-tolerant rats.
Methods:
Randall Selitto and hot-plate tests were utilized to measure the hindpaw withdrawal latencies (HWLs) in response to noxious mechanical and thermal stimuli. To induce chronic morphine tolerance, rats received intraperitoneal morphine injection twice per day for seven days. CaMK II expression and activity were assessed using western blotting.
Results:
Intra-NAc microinjection of autocamtide-2-related inhibitory peptide (AIP) induced an increase in HWLs in naïve rats in response to noxious thermal and mechanical stimuli. Moreover, the expression of the phosphorylated CaMK II (p-CaMK II) was significantly decreased as determined by western blotting. Chronic intraperitoneal injection of morphine resulted in significant morphine tolerance in rats on Day 7, and an increase of p-CaMK II expression in NAc in morphine-tolerant rats was observed. Furthermore, intra-NAc administration of AIP elicited significant antinociceptive responses in morphine-tolerant rats. In addition, compared with naïve rats, AIP induced stronger thermal antinociceptive effects of the same dose in rats exhibiting morphine tolerance.
Conclusions
This study shows that CaMK II in the NAc is involved in the transmission and regulation of nociception in naïve and morphine-tolerant rats.
8.Follow-up of combined intervention for patients with both renal and cerebral artery stenosis.
Chinese Medical Journal 2006;119(9):749-752
Aged
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Angioplasty, Balloon
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Arterial Occlusive Diseases
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diagnostic imaging
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physiopathology
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therapy
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Cerebral Arterial Diseases
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diagnostic imaging
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physiopathology
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therapy
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Cerebral Arteries
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diagnostic imaging
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Female
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Follow-Up Studies
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Humans
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Magnetic Resonance Imaging
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Male
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Middle Aged
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Renal Artery
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diagnostic imaging
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Renal Artery Obstruction
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diagnostic imaging
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physiopathology
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therapy
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Risk Factors
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Stents
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Tomography, X-Ray Computed
9.The study on the expression of caspase-3 in experimental brain contusion in rats.
Lu-yang TAO ; Xi-ping CHEN ; Shi-zhong BIAN
Journal of Forensic Medicine 2004;20(1):9-12
OBJECTIVE:
To investigate the expression of caspase-3 in different posttraumatic intervals and severity of brain injury.
METHODS:
The study examined brain tissue samples of slight (n = 24), severe (n = 24) brain injury and control (n = 6) of rat, using immunohistochemical staining, western-blot and RT-PCR method.
RESULTS:
Up-regulating of caspase-3 expression was found in tissue from traumatic brain injury compared with controls in early 1 hour after TBI, and lasted for 14 days. The gray degree and threshold area of caspase-3 positive cells is different in different severity of brain injury.
CONCLUSION
The increasing of caspase-3 expression indicates that TBI exists. The gray degree, threshold area of caspase-3 positive cells and the cleavage degree of pro-caspase-3 have association with the severity of brain injury.
Animals
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Apoptosis
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Blotting, Western
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Brain Injuries/pathology*
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Caspase 3
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Caspases/genetics*
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Immunohistochemistry
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RNA, Messenger/biosynthesis*
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Rats
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Reverse Transcriptase Polymerase Chain Reaction
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Time Factors
10.Clinical observation on total glucosides of paeony combined with sulfasalazine in treatment of ankylosing spondylitis.
Suo-liang WANG ; Jun-peng WANG ; Hong BIAN
Chinese Journal of Integrated Traditional and Western Medicine 2007;27(3):217-219
OBJECTIVETo evaluate the efficacy and adverse reaction of total glucosides of paeony (TGP) combined with sulfasalazine (SSZ) in the treatment of ankylosing spondylitis (AS).
METHODSSixty-seven AS patients were randomly assigned to 2 groups: the treatment group (34 cases) treated with TGP and SSZ, the control group (33 cases) with methotrexate (MTX) and SSZ. Changes of clinical efficacy related indexes including lumber pain index, morning stiffness time, peripheral joint pain index, thoracic expansion, Schober test, Bath AS disease active index (BASDAI), Bath AS functional index (BASFI), the levels of erythrocyte sedimentation (ESR) and C-reactive protein (CRP), and X-ray of sacroiliac joint were observed.
RESULTSThe clinical efficacy indexes were significantly improved after treatment in the two groups (P < 0.05). Except that the improvement of lumber pain index and peripheral joint pain index was better in the treatment group than that in the control group (P < 0.05), no significant difference was found in the other indexes between the two groups. The occurrence of adverse reation was less in the treatment group than in the control group (P < 0.05).
CONCLUSIONTGP treatment combined with SSZ shows favorable effect on AS with less and milder adverse reaction.
Adolescent ; Adult ; Antirheumatic Agents ; therapeutic use ; Drug Therapy, Combination ; Female ; Glucosides ; therapeutic use ; Humans ; Male ; Paeonia ; chemistry ; Spondylitis, Ankylosing ; drug therapy ; Sulfasalazine ; therapeutic use