Objective:To investigate the effect of telmisartan and fosinopril on the expression of angiotensin-convertion enzyms 2 (ACE2) in cardiomyocyte after myocardial infarction.Methods:Animal model of acute myocardial infarction were established by ligating the left anterior descending coronary artery of rats. Vehicle, telmisartan,fosinopril,or both drugs combined were giv- en to rats by intubation feeding,from 2 weeks before coronary artery ligation to 2 weeks there after. ACE2 protein expression of myocardium were semi-quantified by SP immunohistochemistry. ACE2mRNA were measured by Reverse Transcriptase Poly- merase Chain Reaction (RT-RCR). Results:Compare with sham operated group,ACE2 protein increased significantly in infarc- tion group,but not the ACE2 mRNA. telmisartan and fosinopril therapy caused a significant increase in ACE2 protein compare to infarction group,but only telmisartan caused a significant increase in ACE2 mRNA though all therapied groups showed higher ACE2 mRNA expression. Conclusion:Both telmisartan and fosinopril induced increases in cardiac ACE2 exprssion, whereas the combination of these two drugs do not associated with higher ACE2 expression.

Objective To develop a secure network system to monitor the temperatures in baby incubators.Methods By utilizing patented TAV temperature monitoring system,the running status of temperature-controlled equipments was recorded in digital way,and then it was analyzed and managed dynamically;and real time alarms could be given through networks.Results This system is a kind of security devices independent of baby incubators and can monitor temperatures in baby incubators well.Conclusion This system will not affect original functions of baby incubators,instead it can well monitor the temperatures in both imported and domestic incubators,so that it can improve the safety of facilities being used while decrease medical accidents.

Antibody affinity influences the ant igen-antibody reaction in vivo and in vitro. It is important to routinely determine the antibody affinity in order to elucidate the impacts of this antibody characteristics on the immunoassay and the pathogenetic mechanisms of human diseases. In this paper, a simple method (modified Friguet' s) was reported to directly measure the experimental affinity data of monoclonal antibody in ascites by means of ELISA and to accurately determine the corresponding affinity constants of monoclonal antibody by amendment of underestimation of affinity constants due to the intrinsic properties of Friguet' s, and shows convenience and reliability.

The PTC hot air drying device controlled by a single-chip computer has such advantages as rapid heating, automatic isothermal, weak leakage current and being time-proof, which applies to all calibers of pipeline.

Objective To balance and optimize four key control parameters, acid-base pH, viral spiking(MOI), TPCK trypsin spiking and cell density in MDCK cell suspension culture of influenza virus H3N2, in order to improve the productivity and virus titer of influenza virus H3N2 in MDCK cell suspension culture.Methods A multi-factorial interaction experimental design was created using MODDE~?software of Design of Experiment(DoE) from Sartorius to determine and optimize the infectivity titer of H3N2 virus, considering the interactive effects of four factors: pH, MOI, TPCK trypsin concentration and cell density, aiming to achieve an optimal infectivity titer within a certain range of these four factors.Results The optimal ranges of MDCK cell suspension culture conditions for H3N2 virus were as follows: pH range of 7. 88-8. 00, MOI of 0. 001,TPCK trypsin concentration range of 1. 70 to 6. 0 μg/mL, and cell density range of(55. 70-77. 28 × 10~5) cells/mL. The virus was maintained in Xene-S001 medium at a cultivation temperature of 34. 5 ℃ for 72 h, with a shaking speed of 100 r/min and a CO_2 concentration of 3%. Under these conditions, the TCID_(50) of H3N2 reached 6. 5, and the CV of repeated experiments for TCID_(50) was 1. 03%, with good reproducibility and stability.Conclusion H3N2 virus in MDCK cell suspension culture can achieve higher virus titer.

Several researches have suggested that estrogen contributes to the initiation and development of thyroid cancer by binding to estrogen receptors (estrogen receptor α,estrogen receptor β,and G-protein-coupled receptor 30),activating gene or non-genomic pathways and regulating proliferation of thyroid cells.Studies on antiestrogen drugs based on inhibiting thyroid cell proliferation may provide a new target in treating thyroid cancer.

Child ; Child, Preschool ; Epilepsy, Generalized ; complications ; epidemiology ; genetics ; Female ; Humans ; Infant ; Male ; Pedigree ; Phenotype ; Seizures, Febrile ; complications ; epidemiology ; genetics

OBJECTIVE: To introduce the type and biological properties of medical polymer vehicle materials, and to evaluate its application in anticancer drugs. METHODS: A computer-based on-line research was performed in China Journal Full-text Database and Pubmed database published from 1990 to 2009. The key words were "polymer, anticancer drug, carrier". Articles concerning biological properties of medical polymer vehicle materials and its application in anticancer drugs were included. Meta analysis and repetitive studies were excluded. RESULTS: Quality of articles was assessed, and a total of 24 articles were included. Biological properties of medical polymer vehicle materials and its application in anticancer drugs were summarized. Medical polymer vehicle materials are novel technique with the development of pharmacological study, biomaterial study and clinical medicine. Good biocompatibility, biodegradability, regulation of degradation rate and good workability of polymer materials provided convenience and possibility for innovation of pharmaceutical preparation. Structure of drug carrier material, elevation of drug-loading efficiency, in vivo distribution, biodegradation function and effects of degradation product on bodies deserved further investigations. The study focus of anticancer drug high polymer carrier lies in the search of carrier materials with strong choice and good outcomes. CONCLUSION: Medical polymer carrier materials can control drug release speed by dosage form changes, which induced a stable drug concentration in vivo. The medical polymer carrier materials also can send drugs to a certain part of the body by release system, which cannot affect other regions in the body.

BACKGROUND: The posterior circulation artery is characterized by small caliber, long traveling, many curves and branches, so a stronger resistance will be encountered when a stent is delivered.OBJECTIVE: To investigate the effects of coaxial double catheter technique on intracranial posterior circulation stenting.DESIGN, TIME AND SETTING: A retrospective case analysis was performed at the Department of Neurology, Affiliated People's Hospital of Yunyang Medical College between December 2004 and February 2008.PARTICIPANTS: A total of 21 patients, 11 males and 10 females, aged 50-76 years old, were se/ected from the Department of Neurology, Affiliated People's Hospital of Yunyang Medical College.METHODS: All patients were confirmed as posterior circulation intracranial stenosis (stenosis > 70%) by digital subtraction angiography (DSA), magnetic resonance angiography (MRA), or CT angiography (CTA). They underwent cerebrovascular stent implantation, during which, single catheter technique was first applied, if failed, a coaxial double catheter technique was followed. A conventional group (single catheter technique successfully applied) and a double catheter group (coaxial double catheter technique successfully employed) were set. A written informed consent was obtained from each patient.MAIN OUTCOME MEASURES: Therapeutic results and biocompatibility of implanted stents.RESULTS: The single catheter technique was applied in 21 patients and 6 of them (28.57%) had successful stent deployment, The remaining 15 patients were managed by a coaxial double catheter technique, and the stent was successfully released in these patients. There was only 1 patient presenting with subarachnoid hemorrhage, and the success rate of stent deployment was 93.33%. Chi-square tests results showed that the coaxial double catheter technique produced significantly higher success rate of stent implantation than the single catheter technique (P < 0.05). Except this, no other adverse reactions were found. All stents covered the stenosis lesions completely, and fitted well with the blood vessel wall without rejection, showing the good biocompatibility of selected stents with the host.CONCLUSION: The coaxial double catheter technique can increase the longitudinal supporting strength and enhance the success rate of the intracranial posterior circulation stenting. Meanwhile, the risks of this procedure are low. Therefore, the technique deserves to be popularized.

Objective To discuss the manifestations, diagnosis and management of acute superior mesenteric artery occlusion (ASMAO). Methods The clinical data of 15 cases of ASMAO admitted in our hospital between 2000 and 2007 were analyzed retrospectively. Results The causes of ASMAO were mesenteric artery embolism in 11 patients and mesenteric artery thrombosis in 4 patients. All cases underwent emergency laparotomy, in which 2 cases underwent removal of the embolus, 11 cases were subjected to resection of necrotic bowel after removal of the embolus, and 2 cases gave up definite surgery. Postoperative heparin anticoagutherapy was used in all patients. The overall mortality rate was 27% (4 cases). Conclusions Acute superior mesenterie artery occlusion should be suspected in a patient with sudden abdominal pain, especially in those who have organic cardiac disease. Early diagnosis and prompt embolectomy are the key to save the patients.