1.Cysteinyl leukotriene receptor 1 is involved in rotenone-induced injury of PC12 cells.
Shu-ying YU ; Bing ZHAO ; Xia-yan ZHANG ; Xaio-yan ZHANG ; Yan-fang WANG ; Li-hui ZHANG ; Yun-bi LU ; Er-qing WEI
Journal of Zhejiang University. Medical sciences 2012;41(2):139-145
OBJECTIVETo investigate whether cysteinyl leukotriene receptor 1 (CysLT₁ receptor) is involved in rotenone-induced injury of PC12 cells.
METHODSAfter 24 h treatment with rotenone or with rotenone and the CysLT₁ receptor antagonist montelukast, PC12 cell viability was determined by the colorimetric MTT reduction assay. After PC12 cells were treated with various concentrations of rotenone for 24 h or with 3 μmol/L rotenone for various durations, the expression of CysLT(1) receptor was determined by Western blotting, and its intracellular distribution was detected by immunocytochemistry.
RESULTSRotenone (0.3-30 μmol/L) induced PC12 cell injury; this injury was significantly attenuated by montelukast at 1 and 5 μmol/L.The expression of CysLT(1) receptor increased after rotenone treatment at 1-10 μmol/L, or at 3 μmol/L for 3 and 24 h. Rotenone caused concentration-and time-dependent translocation of CysLT₁ receptor from the nucleus to the cytosol.
CONCLUSIONCysteinyl leukotriene receptor 1 is involved in rotenone-induced injury of PC12 cells.
Animals ; PC12 Cells ; Rats ; Receptors, Leukotriene ; metabolism ; physiology ; Rotenone ; toxicity
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