Objective: To observe the effect of GETO on learning and memory of experiment AD model rats and search its cholinergic mechanism of action.Methods: ?-amyloid protein(A?_(1-42)) injection into hippocampus in rat was applied to make experimental rat model of AD.Morris water maze test were employed to investigate the function of learning and memory in AD model rats after 4 weeks,and immunohistochemical stain was done with Choline Acetyltransferase(ChAT) and nerve growth factor(NGF) antibodies after Morris water maze test,and system of image pro-plus were used to gain the number and grey degree of positive neurons.Results: In Morris water maze test: The mean escape latent period of the Model rats obviously prolonged than that of the Normal rats,the number of traversing flat roof obviously decreased.The mean escape latent period and the number of traversing flat roof of the GETO rats significantly shorten than the model rats.Immunohistochemical test: The number of positive staining neuron in hippocampus CA1 of ChAT and NGF in the GETO rats was not significantly different from those in the Normal rats and the Donepezil rats,but significantly different from those in Model rats with injection of A?_(1-42)(P

AIM: To determine the contents of baicalin, berberine, rhein in Yiqing Capsule(Radix et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis) by HPLC. METHODS: The HPLC system consisted of Kromasil C 18 column (150 mm?4.6 mm, 5 ?m), 0.02 mol?L -1 phosphoric acid (TEA adjusted to pH=6.82)-acetonitrile(40∶11) mixture as mobile phase, detection wavelength at 254 nm, flow rate of 1.0 mL?min -1. RESULTS: The calibration curves of three ingredients were linear within the range of 3.666-73.32 mg?L -1(r=0.9998, baicalin), 0.3112-15.56 mg?L -1(r=0.9998, rhein), 1.221-50.04 mg?L -1(r=0.9999,berberine), respectively. The average recovery of baicalin is 102.3%(RSD=2.5%), berberine for 101.8%(RSD=1.8%), and rhein for 98.3%(RSD=2.9%). CONCLUSIONS: The method is rapid, sensitive and accurate.

BACKGROUND: Abnormal changes of bone mineral density (BMD) easily occur in patients with non-insulin-dependent diabetes mellitus (NIDDM),however the mechanisms are still unclear.OBJECTIVE: To investigate the pathophysiological features of changes of BMD and metabolism in patients with NIDDM from the angle of traditional Chinese medicine (TCM) syndrome types.DESIGN: A case controlled and comparative observation.SETTING: Department of Endocrinology of Guangzhou General Hospital of Guangzhou Military Area Command of Chinese PLA PARTICIPANTS: Totally 103 cases (55 males and 48 females) of patients with NIDDM admitted in Department of Endocrine of Guangzhou General Hospital of Guangzhou Military Area Command of PLA from April 2003 to April 2004 were enrolled. They were, according to TCM syndrome types, divided into 3 groups: yin deficiency with heat excess (32 cases),deficiency of both qi and yin (36 cases), and deficiency of both yin and yang (35 cases) groups. The healthy persons of 30 staff, 16 males and 14 females, in the hospital were concurrently recruited as control group. Informed consent was obtained from all the subjects.METHODS: ①A dual-energy X-ray absorption meter was used to determine the bone mineral densities of orthophoric 1-4 lumber vertebrae, total hipbone and head of femur in left hip in each group. ② Radioimmunoassay was taken to measure the content of serum osteocacin (OC). ③Colorimetry was used to measure the level of serum tartrate resistant acid phosphatase (TRACP). ④ q-test and x2-test were respectively used for comparison of measurement and enumeration data.MAIN OUTCOME MEASURES: ①The BMD of the 1-4 lumber vertebrae, total hipbone and head of femur in left hip in each group. ②The morbidities of osteoporosis of subjects in each group. ③ The levels of OC and TRACP in each group.RESULTS:All 103 NIDDM patients and 30 healthy subjects entered the final result analysis. ① The BMD of the 1-4 lumber vertebrae, total hipbone and head of femur in left hip of subjects: The BMD in yin deftciency with heat excess, deficiency of both qi and yin and control groups were obviously higher than that in deficiency of both yin and yang group (P ＜ 0.05-0.01 ) ② The morbidity of osteoporosis: The morbidities of osteoporosis in yin deficiency with heat excess, deficiency of both qi and yin and control groups were obviously lower than that in deficiency of both yin and yang group[34%(ll/32),42% (15/36), 33% (10/30), 71% (25/35),P ＜ 0.01]. ③ The level of OC: The levels in yin deficiency with heat excess and deficiency of both qi and yin groups were obviously higher than that in deficiency of both yin and yang group (P ＜ 0.01 ), but lower than that in deficiency of both yin and yang group (P ＜ 0.05). ④ The level of TRACP: There was no significant difference among groups(P ＞ 0.05).CONCLUSION: ① The volume and formation of bone in NIDDM patients of deficiency of both yin and yang syndrome type were obviously lower than those in healthy persons and NIDDM patients of yin deficiency with heat excess and deficiency of both qi and yin syndrome types, and the osteoporosis morbidity in them was obviously higher than those in healthy persons and the other two syndrome-type patients. ② The quantity and formation of bone in NIDDM patients of deficiency of both yin and yang syndrome type were near to those in healthy persons.

Purpose To study immunohistochemical quality of prostatic basal cell(PBC). MethodsTherty-seven of basal cell of benign prostatic hypertrophy (BPH) were studied using routine HE stavningand En Vision immunohistochemical methods. Results The positive rate of 34βE12 in PBC were 100 %;but all of the PBC were negative with Vim and SMA; the positive expression of PsAP PSA in PBC were35% ;expression of ck in the PBC was 71% and the positive rate of S-100 in PBC was onty 13.5%.Conclusions The resuts suggest that high molecular weight keratin 34βE12 is a specific marker to PBC;PBC has ability to adenoid epithelia differentiation of prostate and hyperplasia itself. PBC play an importantrole in growth, hyperplasia and disease of prostate. The PBC unlike myoepithelia, the ability to myoepitheliadifferentiation is to go a step further controversial opinion.

Our study showed that the concentration of plasma ammonia in patients with hepatic encephalopathy was increased significantly than that in patients with decompensated liver cirrhosis, primary hepatocellular carcinoma and fulminant hepatitis. The concentration of serum free tryptophan and "free/total" tryptophan ratio in patients with hepatic encepha-lopathy were significantly increased as compared with patients of other three groups. The concentration of serum serotonin was not significantly increased and that of serum 5-hy- droxyindolacetic acid was significantly increased in comparison with normal controls. In addition, the concentrations of serum free tryptophan and serum "free/total" ratio were closely positive correlated with the degree of hepatic encephalopathy, the concentrations of. plasma ammonia and serum free tryptophan and serum "free/total" ratio were higher in patients with hepatic coma than that of patients regainning consciousness, but the difference was not statistically significant. The role of plasma ammonia and serum tryptophan in pathogenesis of hepatic encephalopathy was discussed

Bone marrow mesenchymal stem cells (BMSCs) are a group of pluripotential non-hematopoietic somatic stem cells niched in bone marrow.With the characteristics of stable genetic traits,pluripotential in differentiation,easy to isolate from source tissue,and fast to proliferate when cultured in vitro,BMSCs are currently attracting extensive research interests,and considered to be one of the most promising candidates in corneal tissue engineering.At present,many research groups,domestic and abroad,have reported that BMSCs can not only differentiate into corneal limbal stem cells,corneal epithelial cells,and corneal endothelial cells,but also play an important role in ocular surface repair.However,the successful application of BMSCs in cornea usually depends on the correct selection of supporting materials or scaffold,such as xenogeneic corneal stroma and amniotic membrane.Other unsolved problems in BMSCs-related corneal tissue engineering include the molecular biologic mechanism underlying the directional differentiation from BMSCs to corneal cells,the standards to identify BMSCs from differentiated corneal cells,the optimal scaffold materials and the potential tumorigenicity with grafting of transformed or undifferentiated BMSCs.This paper reviewed the progresses and issues of corneal tissue engineering with BMSCs.

The paper overviewed the research progress of the upper limb rehabilitation training system based on virtual reality technology,introduced some kinds of rehabilitation system, especially the software design, and the application of virtual scene, virtual object and network remote in the upper limb rehabilitation, and summarized the problem of applications of virtual reality in the rehabilitation and put forward the prospects about it.

ObjectiveTo observe the effect of Muscle Stimulating Instrument in enforcing the strength of weaken muscles after selective posterior rhizotomy (SPR). MethodsA group of 27 patients with spastic cerebral palsy aging from 3 to 10 years at the average of 5.7 years were treated with the instrument after SPR. A control group of 36 cases was adopted. Muscle strength, gait and motor function amelioration were compared between two groups. ResultsResults of treatment group in both muscle strength and motor function are better than those of the control group (P<0.05).ConclusionsMuscle stimulating instrument is effective to tone up the muscles which patients felt weak after SPR, and to accelerate the course of rehabilitation.

The article describes the development of new magnetic bead separation and purification instrument. The main application of the instrument is to capture tubercle bacillus from sputum. It is a pretreatment instrument and provides a new platform to help doctors to diagnose bacillary phthisis. Not only could it be used for tubercle bacillus capturing, but also for gene, protein and cell separating and purification. Because the controller of the instrument is 16-bit single chip microcomputer, the cost could be greatly reduced and it will be widely used in China.
Cell Separation ; instrumentation ; Equipment Design ; Magnetics

Tsutsugamushi disease is an acute infectious disease caused by Rickettsia tsutsugamushi.The clinical symptoms of tsutsugamushi disease are varied and non-specific,such as sudden onset fever with chills,rash,skin eschar of ulcer,lymphadenectasis,et al.Patients could combine with multi-organ dysfunction,include respiratory failure,heart failure,mild renal or hepatic dysfunction,circulatory shock or hematological abnormalities.Since the symptoms and signs are non-specific and resemble other tropical infections like malaria,enteric fever,dengue or leptospirosis,appropriate laboratory tests are necessary to confirm diagnosis,and reduce misdiagnosis and missed diagnosis rate.The mainstay of treatment is the tetracycline,chloramphenicol,macrolide and quinolone group of antibiotics.In mild cases,recovery is complete.In severe cases with multi-organ failure,mortality may be as high as 24％.Improve the early diagnosis and timely treatment of the disease,for improving the prognosis of patients,reduce the disease burden is of great significance.