Objective To investigate the effect of amyloid on the expression of kinesin superfamily(KIFs) genes in cortex of Wistar rats. Methods The animal model of Alzheimer's disease was prepared by injection of amyloid into brain ventricle of rats.The learning impairments were verified by water maze test.The levels of mRNA for six kinesin superfamily genes,kinesin,KIFlb,KIFlc,KIF3a,KIF3c,and KIF4,in cortex of rats,were estimated by real-time RT-PCR. Results Two weeks after amyloid injection,the rats showed significant learning impairments.In cortex,the expression of KIF genes was increased after amyloid injection.Conclusion\ The increase of KIF gene may be an evidence for the excitotoxity of amyloid to neurons.

Objective To investigate the moderator of children' s perceptions quality of parental involvement on parenting style and children' s sport expectancy-value beliefs.Methods 595 students of grade 2 to 6 from five elementary schools were chosen and analyzed.Data were analyzed by SPSS16.0 software packet using independent sample t test,One-Way ANOVA,correlation analysis and hierarchical regression statistical analysis of regulation.Results Children' s expectancy beliefs was predicted by authoritarian and behavior encouragement(β=0.076,t =2.35,P=0.019 ;β =0.111,t =3.748,P =0.000).Children' s value beliefs was predicted by affect -warmed and behavior encouragement (β =0.070,t =2.883,P =0.004 ;β =0.070,t =2.745,P =0.006).Children' s sport behavior was predicted by trust-encouragement(β=0.294,t=3.193,P=0.001).The prediction of expectancy beliefs was significant positive by the interaction between indulgence and time involvement (β=0.078,t =2.065,P =0.039).The prediction of expectancy beliefs was significant negative by the interaction between authoritarian and time involvement(β=-0.084,t =-2.454,P=0.014).The prediction of expectancy beliefs was borderline significant positive by the interaction between neglecting and equipment provision(β=0.073,t =1.94,P =0.053).Conclusion Different parenting styles are significant predicators of children sport involvement.Children' s perceptions quality of parental involvement play an important role on parenting style and children' s sport expectancy-value beliefs.

The human sphincter of Oddi has been known for more than a century.The sphincter regulates periodic discharge of bile and pancreatic juice and their directional flow.Research on the sphincter is still inadequate,especially on how to accurately evaluate its function,dysfunction and effective treatment.In recent years,advances in anatomy,regulation and improvements in investigation have deepened our understanding of diagnosis and treatment of sphincter of Oddi dysfunction. This paper summarizes the new developments in this field.

The development of clinical microbiology laboratory is an essential part in promoting the development of clinical microbiology and the basis rational use of antimicrobial agents.Under the circumstances of national specific rectification of antimicrobials,clinical microbiology laboratory should focus on self-buildup and improvement.The development of clinical microbiology laboratory should focus on development of both software and hardware capabilities for lab examination and diagnosis in an all-round way,enhance the antimicrobial susceptibility surveillance,and emphasize and communication and cooperation between laboratory and other departments e.g.clinical departments,pharmacy department and nosocomial surveillance and control office,making concept translation from past ‘paramedical department' to a ‘platform department',contribute its due share to the rational uses of antimicrobial agents.

Objective To explore the clinical effect of hemodialysis combined with hemoperfusion in treatment of secondary hyperparathyroidism. Methods 32 patients with secondary hyperparathyroidism were selected and randomly divided into two groups:the observation group(16 cases) and the control group(16 cases). The patients in the observation group were treated with hemodialysis combined with hemoperfusion, and in the control group, the patients were treated with conventional hemodialysis. Then the levels of serum phosphorus, serum calcium, serum intact parathyroid hormone(iPTH) and skin itching,and weakness and so on were compared. Results After treatment,the level of iPTH in the observation group decreased significantly(P ＜ 0.05) ,but in control group it decreased not obviously, and compared with it before treatment there was no significant difference. After treatment, in the two groups the level of serum phosphorus was significantly lower(P ＜0.05) ,and the level of serum calcium was significantly higher (P ＜ 0.05), and the level of serum phosphorus and iPTH had significant differences between the two groups (P ＜0. 05) ,while no significant difference in serum calcium. In the observation group,skin itching,muscle weakness and other symptoms were improved significantly compared with those in the control group (P ＜ 0.05). Conclusion Hemodialysis combined with hemoperfusion in treatment of secondary hyperparathyroidism was very effective.

Objectives To provide outline for the Research & Development department of hospitals making research strategy by analyzing the quantity of publications and their cited status for the top 20 hospitals in SCI and MEDLINE, and the top 100 hospitals in domestic publications in 2008. Methods The number of publications and their cited times for the first 20 hospitals in SCI and MEDLINE, and the top 100hospitals in domestic publications in 2008 were analyzed with bibliometric analysis. Results Compared with 2007, the increased number and ratio of publications in SCI and MEDLINE for the top 20 hospitals were 1050, 51.19% and 658, 15.04%, respectively; for top 100 hospitals, the average decreased number of domestic publications was 2.08, and the number of cited publications was increased 202.52 and the increased ratio was 13.51%. Conclusions The SCI publications from top 20 hospitals increase sharply, the increase of the MEDLINE articles from the top 20 hospitals is slowly. The negative growth is showed in the number of domestic articles for the top 100 hospitals. In addition, the increase of cited articles is slowly and disproportion.

Objective To explore the expression of cell apoptosis regulatory elements in esophageal canceration course. Methods 60 specimens of esophageal carcinoma and corresponding adjacent no cancerous tissue were detected quantitatively by propidium iodide(PI) stain and indirect immunofluorescence of fluorescein isothiocyanate(FITC) using flow cytometry(FCM) equipment. Results Expression of bcl-2 and c-FLIP in esophageal carcinoma was significantly higher, and Fadd, Caspase-8 and Caspase-3 were significantly lower than that in adjacent tissues (P <0.01). There was positive correlation between Fadd and Caspase-8 gene (P <0.01 or P <0.05). The expression of Fadd was positively correlated to Caspase-8. Comparing with normal epithelium and dysplasia tissue, DNA content and heteroploid cells were increased significantly in esophageal carcinoma. Conclusion During the esophageal canceration course, expressions of relevant cell apoptosis regulatory elements were abnormal. DNA content and heteroploid cells were increased significantly in esophageal carcinoma.

Lipoprotein lipase (LPL) is the rate-limiting enzyme for the hydrolysis of the triglyceride core of circulating triglyceride-rich lipoproteins.LPL can not only affect the lipoprotein level,but also play an important role in the development of atherosclerosis.LPL gene polymorphisms may affect LPL activity,and therefore have both pro-and anti-atherogenic effects.This article reviews the relationship between LPL gene polymorphism and atherosclerosis.

BACKGROUND: The denervated skeletal muscle is chiefly characteristic as the loss of neurotrophic factor, which may cause the muscle atrophy degeneration and fibrosis. OBJECTIVE: To investigate the effect on the muscle satellite cells and muscle atrophy by the implantation of basic fibroblast growth factor (bFGF) released through silicon tube in the gastrocnemius. DESIGN, TIME AND SETTING: Randomly control animal experiment was finished at the Animal Experiment Laboratory of Shenyang Medical College Fengtian Hospital in November 2006. MATERIALS: Twenty-eight Wistar healthy male rats (weighing 250-300 g) were adopted. Silicon tube was prepared by the encapsulation with bFGF or physiological saline, and sealed with 502 glue. METHODS: The sciatic nerve of left lower limb was cut off, and then gastrocnemius was taken out. All of 28 Wistar rats were divided into experiment group and control group randomly, each group involved 14 rats. The experimental gastrocnemius was encapsulated with silicon tube containing bFGF, while the control group with physiological saline. MAIN OUTCOME MEASURES: At day 30 postoperatively, the following indicators were evaluated: wet weight of gastrocnemius and wave amplitude of muscle fibrillation potential; proliferating cell nuclear antigen positive nuclei on surface of muscle fibers, diameter and section area of gastrocnemius fiber under light microscope; ultrastructure of gastrocnemius under electron microscope. RESULTS: The number of muscle satellite cell nuclei in the experimental group was more than that in the control group (P

On the basis of the theories of air sterilization, the Virobuster steritubes are very effective in the removal and inactivation of airborne bacteria species, viral particles, fungi and spore by using the high-intensity UV lamp and close-in UVC treatment room with high reflecting sputtering aluminum, the sterilization efficiency were above 99.999%～99.999 9% when the atmosphere passed through the germicidal tubes. As a technical breakthrough, the Virobuster steritubes could overcome the problems of traditional air sterilization and decontamination technology, the air sterilization could reach LOG-6 sterilizing effect. By analyzing of the workings principles and structure characteristics of Virobuster steritube, the optimization design of air sterilization and decontamination is set up for our public buildings air and hospital sterile room.