Objective To study the clinical significance of the expression of matrix metalloproteinase-9(MMP-9) and E-cadherin(E-cad) in patients with non-small cell lung cancer (NSCLC).Methods Immunohistochemical method (SP) was used to detect the expression of the MMP-9 and E-cad in 62 NSCLC tissues and 18 normal lung tissues.Results The positive expression rate of MMP-9 and the abnormal expression rate of E-cad in NSCLC were closely related to the TNM stages,pathological grades and lymph node involvement (P

Objective:To evaluate the relationship and prognostic significance of matrix metalloproteinase-9(MMP-9)、epidermal growth factor receptor(EGFR) in patients with non- small cell lung cancer(NSCLC). Methods:Immunohistochemical method (S-P) was used to detect the expression of the MMP-9、EGFR in 62 NSCLC tissues and 18 normal lung tissues. Results:The positive expression rates of MMP-9 and EGFR were 67.7%,58.1% respectively in the 62 NSCLC.Their expression levels were significantly higher than those in 18 para-cancerous normal lung tissues(P

Osteosarcoma is the most common primary malignant tumor that develops in bone. Since chemotherapy was introduced into the multi-modality treatment regimen for high-grade osteosarcoma, its prognosis has been impressively improved; about two-thirds of all patients could achieve long-term survival. This review summarized the history of chemotherapy for osteosarcoma and current chemotherapeutic treatment strategies for classical osteosarcoma. The review also emphasized the necessity that the patients should be treated in specialized centers and prospective, multi-institutional trials are needed.

Objective:To investigate 7-11-years-old children's emotional responses, emotional attributions, coping styles to success and failure situation in learning. Methods: With clinical interview method ,90 pupils from seven-years-old to elevelen-years-old were asked to inference to the dramatis personae's affect reaction, emotional attribution in the given situation.Results:Children's emotional response and emotional attribution in learning context is self-regarded, coping style also decline to self effort .There were no age and sex differences in emotional responses to successful situation,there were age differences to failure situation,and there were no sex difference in emotional attribution.Conclusion:Children's emotional responses and emotional attribution to success and failure in learning have different features.

The hardcore of clinical medicine program education is to insist on improving the students'practical capability.The efficient methodology is the key to it.In this paper,the authors investigate the practice of Suzhou University and try to find out the ways to assess the graduates in this program.Also,the authors point out the deficiency in the assessment and put forward mending measures in order to improve the quality of clinical medicine graduate program education.

BACKGROUND:The long-term contraction of skeletal muscle can lead to the decrease of resting potential, affect its contraction, and result in the occurrence of fatigue. Whether acupuncture and injury-free magnetic lifting needle are effective on the recovery of resting potential? OBJECTIVE: To observe and compare the influence on the recovery of resting potential of skeletal muscle by taking acupuncture and magnetic needle as the recovery means.DESIGN: A controlled study.SETTINGS: College of Physical Education, Shanxi Normal University;Binzhou College of Shandong Province.MATERIALS: Thirty experimental toads, which were provided by Beijing University of Physical Education, were randomly divided into acupuncture group (n=10), magnetic needle group (n=10), magnetic needle+pressing group (n=10). Left and right semitendinosus muscles were taken as controls.METHODS: The in vitro toad's semitendinosus muscles (experimental side and control side) were treated with electrostimulation for 40 minutes,and then the resting potential of skeletal muscular cells were measured with the intracellular glass microelectrode technique. After the electrostimulation, filiform needle acupuncture, magnetic needle, magnetic needle plus pressing were also given.MAIN OUTCOME MEASURES: The resting potentials were observed before exercise, after electrostimulation and other treatments in the groups.RESULTS: ① The resting potentials of skeletal muscular cells after electrostimulation were decreased as compared with those before electrostimulation [experimental side: (59.48±7.37), (84.54±5.72) mV; control side:(59.43±7.14), (83.62±5.86) mV]. ② After the treatments of acupuncture,magnetic lifting needle, magnetic lifting needle plus pressing, the decreased resting potentials after electrostimulation were obviously recovered (P ＜ 0.01). ③ The recovered percentage of resting potential in the magnetic needle group was obviously smaller than those in the acupuncture group and magnetic needle+pressing group [(42.50±12.41)%, (53.71±10.93)%,(49.10±8.39)%, P ＜ 0. 01].CONCLUSION: Acupuncture, magnetic lifting needle, magnetic lifting needle plus pressing plays a role in promoting the recovery of resting potential of skeletal muscular cells after long-term electrostimulation, and the effect of simple magnetic needle is smaller than that of acupuncture and magnetic needle plus pressing.

Objective: To investigate mandibular movement characteristics in patients with skeletal class Ⅲ malocclusion during chewing different foods. Methods: 14 skeletal class Ⅲ adult patients with mandibular asymmetry, 10 patients without mandibular asymmetry and 10 normal control cases were chosen to find out the different condylar and incisor movement area ratio based on the aid of ARCUSdigma mandibular moving track analysis system. Results: First,during chewing soft food, the condylar tracing length of skeletal class Ⅲ adult patients with mandibular asymmetry were significant different in sagittal and horizontal plane compared with the other two groups(P<0.05), while the incisor point trajectory difference was not statistically significant. Second, the condylar and incisor movement characteristic showed much more different among three samples during chewing brittle food(P<0.05). Third, in sagittal plane, the deflective condylar movement area ratio was significantly higher than that of the other two groups during chewing hand food(P<0.01). Conclusion: The mandibular movement in skeletal class Ⅲ adult patients are affected obviously by the shape and texture of food. Asymmetric lower mandible patients have a greater preponderance of this phenomenon.

Objective To explore the expression of Aurora-A in breast carcinoma and the relationship with breast carcinoma treatment efficacy of paclitaxel. Methods The expression of Aurora-A were tested by immunohistochemical SP method in 67 cases of breast carcinoma. The relationship of Aurora-A with treatment efficacy of paclitaxel was analysed. Results The expression rate of Aurora-A was 73.13%(49/67)in breast carcinoma. The treatment effective rate of breast carcinoma was 77.78%(14/18) and the rate of nonresponder was 11.11%(2/18) in negative expression of Aurora-A;but the treatment effective rate was 34.69%(17/49) and the rate of nonresponder was 34.69%(17/49) in positive expression of Aurora-A. There were obvious correlations between expression of Aurora-A and treatment efficacy of paclitaxel. Statistical significance could be found between them (P = 0.013 ). Conclusion There is manifest correlation between the high expression of Aurora-A and resistance of paclitaxel in breast carcinoma.

Objective Protein chip for mucin detection was prepared to detect the serum levels of mucin1(MUC1),mucin2(MUC2)and micin5ac(MUC5AC)of colorectal cancer patients,and to discuss the relationship between colorectal cancer and the expression of MUC1,MUC2 and MUC5AC in serum.Methods The protein chip was prepared with monoclone and polyclone antibodies for the measuement of the serum levels of MUC1,MUC2 and MUC5AC in 30 patients with colorectal cancer and 30 healthy adults as control,using the method of immuno-fluorescence.Results The serum levels of mucin and CEA in colorectal cancer group were higher than that in control group.The differences were significant between the two groups concerning MUC1(5.25?2.37 vs 3.65?1.82U/ml,P0.05).The positive expression of mucin was related with the cancer differentiation.The positive expression of MUC1 and MUC5AC was higher when cancer differentiation was low as compared with high and median differentiation,but the positive expression of MUC2 was higher in high and middle cancer differentiation.When the serum level of mucin was used as an indicator for the diagnosis of colorectal cancer,the sensibility and specificity were both higher.The sensibility and specificity of MUC1 were 82.1% and 78.1%,respectively,while of combined mucins(at least 2 in 3 mucins were positive)were 96.2% and 85.3%,respectively.Conclusion The application of protein chip for mucin detection on the measurement of serum levels of MUC1,MUC2 and MUC5AC is of higher sensibility and specificity in the diagnosis of colorectal cancer.The establishment of protein chip has provided a new approach to early diagnosis of colorectal cancer,and help estimate the prognosis.

Objective To establish a method for quantitative detection of hepatitis B virus covalently closed circular DNA(HBV cccDNA ) in infected cells. Methods The transfected cell line HepG2.2.15 which can consistently produce Dane particles was maintained in DMEM containing 380 ?g/ml G418 and 10% fetal bovine serum. Cells in the exponential period were harvested from flasks, then intracellular HBV cccDNA was extracted from pellet containing 1?10~6 cells with mini plasmid extraction kit (QIAGEN).The extraction product was further purified by mung bean nuclease to remove HBV relaxed circular DNA possibly remained. HBV cccDNA was quantitatively detected by fluorescent PCR with selective primer set and Taqman MGB probe. Culture medium before exponential period, HBV DNA positive and negative serum samples from patients with chronic hepatitis B (mild) were amplified simultaneously to test the specificity of the fluorescent PCR method. Plasmids containing whole HBV genome were amplified with the same primer set and fluorescent probe to determine the sensitivity of the method. Results HBV cccDNA was detectable in HepG2.2.15, and the average quantity was 18 copies per cell approximately. No detectable fluorescent signal was observed when culture and serum samples were amplified. The detectable HBV cccDNA was as low as 10~3 copies per ml at least by this method. Conclusions This method is convenient, highly specific and highly sensitive. It can be utilized in the quantitative detection of intracellular HBV cccDNA as well as in the screening and evaluation of antiviral agents.