1.Further improve the quality of epidemiological survey of eye diseases
Chinese Journal of Experimental Ophthalmology 2013;31(10):905-907
Public Health Ophthalmology is a population-,region-or country-based as a whole object,and is committed to improving the eye health of the whole population.Epidemiological investigation of the eye was named to the region from a single disease to variation of diseases from cross-sectional survey to a longitudinal cohort study.Epidemiological investigations is the most important way which can reflect the pathogenic factors for public health policy-making,early diagnosis and prevention and treatment of eye disease,and Research to Prevent Blindness.Imaging Diagnosis,quality control,improve the reliability of the epidemiological investigation,and to strengthen the study of disease,race,and molecular levels and create a unique epidemiological study.
2.Medical Ethics Education for Clinical Interns in Minorities Area
Shihua XU ; Leban NONG ; Jie WEI
Chinese Medical Ethics 1995;0(04):-
Clinical practice is an important process for the formation of the clinical skills and decent medical ethics of medical students.This paper clarifies various issues arising in the current medical ethics education,and proposes relevant countermeasures from the perspectives of medical colleges,training hospitals,guidance teachers,and so on.
3.Roles of cell signaling pathways in thyroid carcinoma
Yingkai XU ; Jie HU ; Wei LIU
Journal of International Oncology 2015;42(12):911-915
The intracellular signal transduction pathways play an important role in the occurrence and development of thyroid cancer.Mitogen activated protein kinase (MAPK) signal transduction pathway,phosphatidylinositol 3-kinase (PI3K)-AKT pathway,NF-κB pathway and RASSF1-MST1-FOXO3 pathway affect the occurrence and development of thyroid cancer by modulating the activity and expression of specifical proteins,and determine the growth and apoptosis of thyroid cancer cells.
5.Role of AMP-activated protein kinase in regulating LPS-induced secretion of inflammatory cytokines in mouse alveolar macrophages
Wei WANG ; Xiaoguang LI ; Jie XU
Chinese Journal of Microbiology and Immunology 2015;35(7):502-505
Objective To investigate the mechanism of 5′adenosine monophosphate-activated pro-tein kinase ( AMPK) in the regulation of LPS-induced secretion of inflammatory cytokines in mouse alveolar macrophages.Methods Alveolar macrophages were isolated from wild type and AMPKα1-/-C57BL/6J mice.ELISA was used to measure the concentrations of IL-1βand TNF-αin the culture supernatants of LPS treated alveolar macrophages with or without 5-amino-1-β-D-ribofuranosyl-imidazole-4-carboxamide ( AICAR ) stimulation.Western blot assay was performed to analyze the expression of AMPKα1 and AMPKα2 in alveolar macrophages isolated from wild type and AMPKα1-/-mice as well as the LPS-induced changes of p-AMPKαactivity in wild type mice.Results The LPS-induced secretion of inflammatory cyto-kines in alveolar macrophages isolated from AMPKα1-/-mice were significantly higher than that of wild type mice.The activities of p-AMPKαin wild type mice were suppressed by LPS treatment.Treatment of AICAR inhibited the LPS-induced secretion of TNF-αand IL-1β.Conclusion The LPS-induced secretion of in-flammatory cytokines was enhanced in AMPKα1-deficient mice, but could be suppressed upon the treatment of AICAR, an agonist of AMPK.AMPK was involved in the regulation of LPS-induced secretion of inflamma-tory cytokines by alveolar macrophages in mice.
6.Inhibition of LPS-induced expression of myeloid differentiation factor 88 by fenoterol is associated with its anti-inflammatory effect
The Journal of Practical Medicine 2014;(18):2890-2893
Objective To explore the molecular mechanism of inhibition of LPS-induced inflammation by fenoterol, a β2 adrenoceptor agonist in monocyte. Methods Concentrations of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α) and MCP-1 from cell supernatants from THP-1 cells and wild type or MyD88- / - mice peritoneal macrophages stimulated by LPS in the presence or absence of fenoterol were determined by use of an ELISA system. Expression of MyD88 (myeloid differentiation factor 88) stimulated by LPS in the presence or absence of fenoterol were determined by Western blot. Results Fenoterol inhibited LPS-induced activation of MyD88 and secretion of inflammatory cytokines (TNF-α, MCP-1, and IL-1β). The reaction of MyD88- / - mice peritoneal macrophages to LPS was much lower than that of the wild type mice peritoneal macrophages. Conclusions MyD88 plays an important role in inflammation induced by LPS. The inhibition of LPS-induced expression of MyD88 by fenoterol is associated with its anti-inflammatory effect.
7.Expression of Twist in Middle and Advanced Stages Cervical Cancer and Its Clinical Significance
Jie WEI ; Qin XU ; Lihua YANG
Journal of Kunming Medical University 2014;(1):25-27
Objective The purpose of this study was to research the expression of Twist in cervical cancer tissues and the relationship between its expression and clinicopathologic characteristic, and to investigate the role of twist in cervical cancer invasion, metastasis and progress. Methods Immunohistochemistry was used to detect the expression of the Twist in 78 cases of middle and advanced stages cervical cancer tissue microarray chip. The relationship between its expression and clinicopathological characteristic was analyzed. Results (1) The positive expression of twist in cervical cancer was 56/78 (71.8%);(2) The expression of Twist in cervical cancer was significantly related to tumor histology type, FIGO stage, lymph node metastasis and differentiation degree. With the increase of FIGO stage, the emergence of lymph node metastasis and the decrease of differentiation degree, the expression of Twist increased gradually ( <0.05) . Conclusion The expression of Twist may be closely related to the invasion, metastasis and progress of cervial cancer.
8.Au@PVP Core-Shell Nanoparticles Used as Surface-Enhanced Raman Spectroscopic Substrate to Detect Malachite Green
Ningning XU ; Qin ZHANG ; Wei GUO ; Qintao LI ; Jie XU
Chinese Journal of Analytical Chemistry 2016;44(9):1378-1384
The core-shell nanopaticles of Au@polyvinyl-pyrrolidone ( PVP) with uniform size and controllabe shell-thickness were prepared by hydrothermal method. The core-shell nanoparticles could be assembled to be the monolayer array on Si substrate relying on the dispersion of core-shell nanoparticles arising from PVP shell. The malachite green ( MG ) absorbed by H-bond could be detected on the array under the electromagnetic enhancement of inner-core Au nanoparticles. Under the conditions of the optimum shell-thickness of Au@PVP and the appropriate absorbed time of MG, the detection of MG could be realized in the linear range from 1 × 10-10 mol/L to 1 × 10-5 mol/L with the correlation coefficient ( R2 ) of 0. 98. The detection limit was 10-12 mol/L. This method was applied to the determination of MG in tilapia fish fillets of Xiagang market. No MG was found in this real sample. The spiked recoveries of the sample ranged from 70. 8% to 126. 0%. This method is simple and accurate, and can be used for detection of MG in the fish.
10.A Study on Genetic Factors in Patients with Vitiligo
Xiukun SUN ; Aie XU ; Jie OUYANG ; Xiaodong WEI
Chinese Journal of Dermatology 2003;0(09):-
Objective To investigate family history, sex, age of onset, disease severity and environmental predisposing factors in vitiligo patients. Methods Eight hundred and fifteen vitiligo patients were investigated by questionnaires. Patients with family history were compared with those without such history. SPSS 10.0 software package was applied for data analysis. Results Of 815 vitiligo probands, 128 had family history and 687 did not. The heritability rate was 15.7%. Compared with general population, vitiligo probands with affected fathers or mothers had a relative risk (RR) of 132 or 72, respectively. The RRs of those with affected first-degree relatives varied from 12 to 28. There was no significant difference of mean age of onset and disease severity between patients of paternal inheritance and maternal inheritance. No significant difference was found regarding sex and mean age of onset between the groups with and without family history. However, the patients with family history were more likely to have scattered, bilateral distributed and progressive vitiligo. Of the environmental predisposing factors, the mean daily sun-exposure time was closely related to vitiligo in patients with family history. Conclusion Genetic factors may play an important role in the pathogenesis and disease severity of vitiligo.