Objective To present study was to investigate the effects of insulin-like growth factor binding protein 7 (IGFBP7)on the proliferation of human hepatocellular carcinoma HepG2 cells.Methods Human hepatocellular carcinoma HepG2 cells was cultured,and plasmid pIRES2-ZsGreen1-IGFBP7 or empty plasmid was transfected into HepG2 cells and the cell transfection efficiency was examined by fluores-cence microscopy;MTT was performed to evaluate the effect of IGFBP7 on proliferation and apoptosis of HepG2 cells in 48 hours after transfection.Results IGFBP7 transfected group decreased cell proliferation noticeably.Conclusions Overexpression of IGFBP7 can down-regulte the proliferation of human hepatocel-lular carcinoma HepG2 cells.

Background and objective Radiotherapy is an important treatment for lung cancer. The poor prognosis of lung cancer is largely caused by the high recurrence rate and metastasis of the tumor. Autophagy, which can be induced by radiotherapy, might be associated with DNA repair. The aim of this study is to investigate whether activating autophagy us-ing rapamycin can enhance the radiosensitivity of lung cancer cells and clarify the association of autophagy with DNA repair. Methods The human adenocarcinoma A549 cell line was selected as the experimental subject. The specimens were divided into three groups: control (N), radiation (R), and Rapamycin and radiation (R+ARPA). hTe protein levels of γ-H2AX, Rad51, Ku70/Ku80, p62, and LC3 were determined by Western blot. Autophagosome was observed under a transmission electron microscope, and SF was determined by colony formation assay.Results Compared with group R, the activity of autophagy and the protein expression levels of Rad51 and Ku70/80 were remarkably increased in group R+ARPA.ConclusionhTe radiosensitivity of lung cancer can be promoted by activating autophagy via treatment with Rapamycin, and the process may be associated with DNA repair.

This article explains the concept of completion ability, summarizes the measurement tools of nursing students' completion ability and the status of completion ability, and analyzes the influencing factors of nursing students' completion ability from the levels of personal, school, and clinical practice, aiming to explore scientific and effective training models and intervention measures to provide reference for future research.

Objective:To explore the mediating effect of adversity quotient between transition shock and work readiness among newly graduated nurses.Methods:This is a cross-sectional study. From May to June 2021, a total of 242 newly graduated nurses from three ClassⅢ Grade A hospitals in Shandong Province were selected as the research objects by the convenient sampling method. The general situation questionnaire, Transition Shock Scale of Newly Graduated Nurses (TSS-NGN) , The Adversity Response Profile (ARP) and Work Readiness Scale for Graduate Nurses (WRS-GN) were used to survey. Pearson correlation analysis was used to test the correlation between transition shock, adversity quotient and work readiness AMOS 23.0 was used to establish the structural equation model. A total of 242 nurses were investigated in this study. 228 valid questionnaires were collected, and the effective recovery rate was 94.21%.Results:Among 228 newly graduated nurses, the total scores of work readiness, transition shock and adversity quotient were respectively (279.04±47.73) , (81.43±22.22) and (132.39±15.00) . Transition shock was negatively correlated with adversity quotient and work readiness ( r=-0.307, -0.291; P<0.01) , and adversity quotient was positively correlated with work readiness ( r=0.339, P<0.01) . The results of structural equation modeling showed that adversity quotient partially mediated between transition shock and work readiness (β=-0.243, P<0.01) , and the mediating effect accounted for 38.76% of the total effect. Conclusions:The work readiness of newly graduated nurses is at the upper middle level. Clinical nursing managers should pay attention to cultivating the adversity quotient of newly graduated nurses, so as to reduce the negative effect of transition shock on their work readiness, in order to realize a good transition from school to clinical nursing work.