Children with primary vesicoureteral reflux(VUR) are often complicated with urinary tract infection and voiding dysfunction.It often leads to kidney scarring,renal atrophy,hypertension,kidney dysfunction and a series performance of reflux nephropathy.In serious cases it can even progressed to end-stage renal disease.Now,with the accumulated experience of VUR treatment,the diagnosis strategy and the treatment remains certain amount of controversy.Whether the surgery or non-surgical treatment,the results are still not satisfactory,the pathogenesis of VUR is still unclear,the further study and exploration for the pathogenesis of VUR is required.

Objective To investigate the effect of hypoxia/reoxygenation on apoptosis of rat hypertrophic cardiomyocytes and the metabolic pathway changes of hypertrophic cardiomyocytes by dichloroacetate.Methods The isolated ventricular cardiomyocytes from neonatal Wistar rat were cultured and purified with differential attachment,then BrdU was added to reduce the rate of non-myocytes.The cultured cells were identified by morphology,spontaneous contraction and specific immunocytochemical stain.Hypertrophic cardiomyocyte model induced by angiotensin Ⅱ was set up and assessed by -Leu incorporation.The cultured media were replaced by low glucose DMEM before hypoxia.The cardiomyocytes were incubated at 37 ℃ in an air-tight incubator containing 92% N_(2),5% CO_(2),3% O_(2) for 24 h to simulate hypoxia,then under the condition of 23% O_(2),5% CO_(2) for 4 h to simulate reoxygenation.Apoptotic cells was evaluated by a modified TUNEL assay(DeadEnd~(TM) Colorimetric TUNEL).Results The apoptotic rate of cardiomyocytes increased with time prolongation of hypoxia/reoxygenation(H/R).The apoptotic cell model induced by H/R in rat hypertropic cardiomyocytes was established successfully.When the hypertropic cardiomyocytes were pretreated with Dichloroacetate,the apoptotic rate decreased in a dose-dependent manner.The metabolic pathway changes had effect on the apoptosis of rat hypertrophic cardiomyocytes.Conclusion By collagense digestion,it is convenient to obtain primary neonatal Wistar rat ventricular cardiomyocytes. This method is one of vital research tools in the fields of cardiac hypertrophy.Angiotensin Ⅱ could accelerate cellular proliferation,structural protein biosynthesis and cardiac hypertrophy.Dichloroacetate has the effect of antiapoptosis.

Objective:To explore the clinical curative effect of Gankening in treating chronic nonproductive cough based on syndrome differentiation.Method:90 cases were allocated to two groups randomly,the treatment group(60 cases) treated with Gankening based on syndrome differentiation and the control group(30 cases) treated with Zhisou Powder and Beilai.The composite effect of the two groups,the curative effects of every syndrome pattern in the treatment group before and after treatment and the relationship between the symptom index and the curative effect were observed.Result:The clinical effects of the treatment group were markedly exceeded the control group.In the treatment group,the clinical control rate was 53.33%(32 cases),markedly effective rate was 30%(18 cases),the(effective) rate was 13.33%(8 cases) and the total effective rate was 96.67%.There was distinct difference in the treatment group before and after treatment(P

Nearly half a century,an increasing number of studies have found that the puberty of human being is at early trends.Puberty is the process of physical changes involving reproductive system maturation and the acquisition of fertility,by the combination effects of nervous system,endocrine system and the environment.Pubertal timing is a relative concept and the process of pubertal development can be shown as early,timely or relatively late as compared with a reference group.This article mainly focuses on related influencing factors of pubertal timing including genetic factor,childhood obesity,growth pattern (intrauterine growth retardation,catchup growth,adiposity rebound),psychosocial stress (poor family emotional environment,father absence,international adoption),environmental endocrine disrupting chemicals with their mechanism and significance.The exploration of relevant risk factors of early puberty can provide scientific evidence for formulating relevant policies and targeted prevention.

The fractional exhaled nitric oxide(FeNO)has been applied more and more widely in disorders of respiratory system,and is considered to be one of the most promising markers of expiration in monitoring the development and outcome of respiratory inflammatory airway disease.This paper is to review the application and prospect of FeNO in bronchiolitis in children.

Objective To observe the effect of ejiao huangqi koufuye and iron sucrose injection in the treat-ment of postpartum anemia,to provide reference for clinical treatment.Methods 100 postpartum anemia women met the inclusion were randomly divided into 50 cases of observation group and 50 cases of control group.The control group was given iron sucrose injection 200mg for 3d,the observation group was given ejiao huangqi koufuye for 30d, twice daily and 10ml once.The blood was exsanguinated for detecting RBC,Hb,HCT,SF,TIBC.The improvement of symptoms and signs were observed and the effect was assessed.Results The RBC,Hb,HCT,SF of the observation group and control group after treatment were (2.14 ±0.74)×10 12 /L and (3.32 ±1.15 )×10 12 /L,(97.85 ± 22.44)g/L and (109.72 ±18.54)g/L,(36.42 ±8.39)% and (41.10 ±7.38)%,(22.26 ±1.76)mg/L and (32.2 ±2.1)mg/L,which were significantly higher than before treatment (t =3.065 and 5.614,3.411 and 3.961, 3.317 and 5.062,6.448 and 9.332,both P <0.05 ),TIBC were (70.82 ±13.23 )μmol/L and (62.41 ± 13.37)μmol/L,which were significantly lower than before treatment(t =4.607 and 7.302 respectively,all P <0.01).After treatment,the RBC,Hb,HCT,SF of the observation group were significantly higher than the control group,the TIBC was significantly lower than the control group,the differences were statistically significant(t =3.826, 3.733,3.395,4.776,3.524,all P <0.05).The total effective rate of the observation group was 94%,which was sig-nificantly higher than 82% of the control group,the difference was statistically significant(χ2 =8.401,P <0.05). The incidence rate of adverse reactions of the observation group was 4% (2 /50),which of the control group was 6%(3 /50),the difference was not statistically significant(χ2 =0.211,P >0.05).Conclusion The ejiao huangqi kou-fuye and iron sucrose injection has exact clinical efficacy in the treatment of postpartum iron deficiency anemia,can effectively promote bone marrow erythropoiesis,anemia symptoms,it is worthy of clinical use.

Objective:To compare the efficacy and safety of Novoiaped 30 and NovoMix 30 Flexpen in the therapy of type 2 diabetes mellitus(DM). Methods:A multicenter,randomized,and controlled clinical trial of Novorapid 30R and Novomix 30 Flexpen was performed in XinQiao Hospital in Chongqing China. One hundred and fifty patients with type 2 DM received Novorapid 30R therapy and sixty patients received NovoMix 30 Flexpen for 12 weeks respectively. Results:(1)At the end of 12-week treatment,significant improvements in HbA1C and fasting plasma glucose(FPG) levels were shown in both groups, HbA1C decreased 1.08% and 1.01%,and FPG decreased 2.0mmol/L and 2.2mmol/L,in Novorapid 30R and NovoMix 30 Flexpen group respectively(all P0.05). (2)2hpG decreased 3.79mmol/L and 3.05mmol/L,Novorapid 30R was better than Novomix 30 Flexpen,The difference was statistically significant between two groups(P

Objective To observe the change of serum anti-brain antibodies after cardiopulmonary resuscitation,and investigate the clinical significance.Methods A total of 27 cases of cardiopulmonary resuscitation after cardiac arrest patients with success and survival more than 12 weeks were divided into two groups according to Glasgow Coma Scale (GCS) score:mild and moderate group with GCS score ≥ 8 scores (12 cases) and severe group with GCS score≤7 scores (15 cases).The serum anti-brain antibody levels at 3,7 days and 2,3,4,12 weeks after recovered in the spontaneous circulation (ROSC) was compared between two groups,and compared with control group (15 cases of healthy persons).Results The serum anti-brain antibody levels at 3,7 days and 2,3,4,12 weeks after ROSC were significantly higher than those in control group [(1.34 ± 0.23),(1.30 ± 0.27) kU/L vs.(0.28 ± 0.05) kU/L,(1.38 ± 0.33),(1.44 ± 0.30) kU/L vs.(0.28 ±0.05) kU/L,(1.44 ±0.31),(1.51 ±0.33) kU/L vs.(0.28 ±0.05) kU/L,(1.53 ±0.27),(1.67 ±0.36) kU/L vs.(0.28 ±0.05) kU/L,(1.72 ±0.25),(1.93 ±0.44) kU/L vs.(0.28 ±0.05) kU/L,(1.98 ±0.45),(2.15 ±0.52) kU/L vs.(0.28 ±0.05) kU/L],and there were significant differences (P＜ 0.01).The serum anti-brain antibody levels in severe group were significantly higher than those in mild and moderate group,and there were significnat differences (P ＜ 0.05).Conclusions The serum anti-brain antibody levels after cardiopulmonary resuscitation rise significantly.Anti-brain antibody may be used as a biochemistry marker to judge degree and prognosis of brain injury with patients after cardiopulmonary resuscitation.

Liver cirrhosis is the advanced stage of various chronic liver diseases,and its complications include portal hypertension,esophageal and gastric varices,hepatocellular carcinoma,etc. Transient elastography( TE),a noninvasive method for liver stiffness measurement( LSM),has been used widely to detect liver fibrosis qualitatively and quantitatively. Recently,correlations were observed for LSM with liver cirrhosis and its complications. This article reviewed the studies focused on these correlations,as well as the clinical application of TE.

With the development of mass spectrometry technology and its wide range of applications in the biological field,Veristrat classifier has been applied in non-small cell lung cancer (NSCLC) individualized therapy as well as in other tumors.Oncologists select individualized treatment plan based on peripheral blood veristrat test results.Many trials have verified its guiding role in NSCLC and other tumors' s first line and multi-line individualized therapy.Veristrat classifier provides a new idea and method for individualized treatment of NSCLC and other tumors.